• Clinical and Experimental Pediatrics
• /
• 제50권9호
• /
• pp.905-911
• /
• 2007

목 적 : Ghrelin은 위에서 주로 분비되는 펩타이드로, 성장호르몬의 분비를 촉진시키고, 식욕을 증가시켜 에너지 균형을 조절한다고 알려져 있으며, leptin은 지방세포에서 분비되어 식욕을 억제시키는 작용을 통해 체중 및 에너지 대사에 관여한다. 저자들은 소아 종양으로 진단받고 항암 요법중인 환아의 ghrelin과 leptin 농도를 정상아와 비교해 보았다. 방 법 : 2003년 12월부터 2004년 12월까지 강남 성모병원과 성모병원에서 소아 종양을 진단 받고 방사선 조사나 항암 화학요법을 받은 환아들 중 43명(남아 31명, 여아 12명)을 대상으로 하였으며, 환아군과 성별, 나이, 체중 및 신장이 비슷한 건강한 소아 45명(남아 26명, 여아 19명)을 대조군으로 하였다. 대상아들의 혈중 leptin과 ghrelin 농도를 방사면역측정법으로 측정하였다. 결 과 : 환아군과 대조군에서 BMI와 leptin 농도는 환아군에서 더 높았으며, ghrelin은 대조군에서 더 높았다. 또한 leptin 농도는 환아군(r=0.61, P<0.05)과 대조군(r=0.40, P<0.05) 모두에서 BMI와 양의 상관관계가 있었다. ghrelin 농도는 정상군에서는 연령, 신장, 체중 및 BMI와 음의 상관관계를 보였으나 환아군에서는 의미있는 상관관계는 없었다. 한편, 방사선요법을 받은 군과 화학요법만을 받은 군으로 나누어 비교했을 때에는 두 군간의 의미있는 차이는 없었으며, 급성 골수성 백혈병군과 급성 림프구성 백혈병군으로 나누었을 때 ghrelin의 농도가 골수성 백혈병군에서 훨씬 높았다. 결 론 : 소아 종양으로 항암 요법을 받고 있는 환아는 정상아에 비해 BMI가 더 높고, leptin 농도도 더 높았으나, ghrelin 농도는 더 낮았다. 그러나 본 연구가 한 시점에서의 단기적이고 단편적인 연구이므로 추후 오랜 기간에 걸친 전향적인 연구가 더 필요하다고 생각된다.

• Genomics & Informatics
• /
• 제12권1호
• /
• pp.21-34
• /
• 2014

A visual analysis approach and the developed supporting technology provide a comprehensive solution for analyzing large and complex integrated genomic and biomedical data. This paper presents a methodology that is implemented as an interactive visual analysis technology for extracting knowledge from complex genetic and clinical data and then visualizing it in a meaningful and interpretable way. By synergizing the domain knowledge into development and analysis processes, we have developed a comprehensive tool that supports a seamless patient-to-patient analysis, from an overview of the patient population in the similarity space to the detailed views of genes. The system consists of multiple components enabling the complete analysis process, including data mining, interactive visualization, analytical views, and gene comparison. We demonstrate our approach with medical scientists on a case study of childhood cancer patients on how they use the tool to confirm existing hypotheses and to discover new scientific insights.

• Pediatric Infection and Vaccine
• /
• 제8권2호
• /
• pp.241-246
• /
• 2001

저자들은 급성 림프구성 백혈병으로 진단받고 항암 약물 치료 중인 10세 남아와 선천성 수신증으로 진단받고 도뇨관 유치 후 배뇨성 방광 요도 조영술을 촬영한 신생아에서 발생된 Burkholderia cepacia 패혈증을 혈액 배양 검사로 진단하고 이에 감수성이 있는 항생제 투여 후 임상적으로 호전을 보였기에 문헌고찰과 함께 보고하는 바이다.

• 대한한의학방제학회지
• /
• 제25권3호
• /
• pp.349-362
• /
• 2017

Objectives : We investigated whether the components of Ailanthus altissima induced apoptotic cell death in Jurkat acute lymphoblastic leukemia (ALL) cells. Methods : Regulation of cell proliferation is a complex process involving the regulated expression and/or modification of discrete gene products, which control transition between different stages of the cell cycle. Results : Upon treatments with Ailanthus altissima, the concentration-dependent inhibitions of cell viability were observed as compared to untreated control group. The capability of Ailanthus altissima to induce apoptosis was associated with proteolytic cleavage of specific target proteins such as poly(ADP-ribose)polymerase (PARP) and beta-catenin proteins suggesting the possible involvement of caspases. Ailanthus altissima also caused apoptosis as measured by cell morphology and DNA fragmentation. Conclusions : These results indicate that the increase of apoptotic cell death by Ailanthus altissima may be due to the inhibition of cell cycle in human Jurkat lymphocytes. Conclusively, these current and further findings will provide novel approaches to understanding and treating major diseases.

• Bulletin of the Korean Chemical Society
• /
• 제35권12호
• /
• pp.3571-3575
• /
• 2014

Proteasome, a multicatalytic protease complex, has been validated as a promising therapeutic target in oncology. Carfilzomib (Kyprolis$^{(R)}$), a tetrapeptide epoxyketone, irreversibly inhibits the chymotrypsin-like (CT-L) activity of the proteasome and has been recently approved for multiple myeloma treatment by FDA. A chemistry effort was initiated to discover the compounds that are reversibly inhibit the proteasome by replacing the epoxyketone moiety of carfilzomib with a variety of ketones as reversible and covalent warheads at the C-terminus. The newly synthesized compounds exhibited significant inhibitory activity against CT-L activity of the human 20S proteasome. When the compounds were tested for cancer cell viability, 14-8 was found to be most potent in inhibiting Molt-4 acute lymphoblastic leukemia cell line with a $GI_{50}$ of $4.4{\mu}M$. Cytotoxic effects of 14-8 were further evaluated by cell cycle analysis and Western blotting, demonstrating activation of apoptotic pathways.

• Investigative Magnetic Resonance Imaging
• /
• 제23권4호
• /
• pp.390-394
• /
• 2019

Hemosiderosis is characterized by the deposition of excess iron in body tissues. The choroid plexus is an important part of the central nervous system that can be the primary site of iron overload. T2*-weighted gradient echo (GRE) sequence provides high sensitivity for demonstrating cerebral microhemorrhagic foci and iron deposition. In the present study, we describe the case of a 15-year-old boy with acute lymphoblastic leukemia, in whom repeated transfusion led to iron accumulation in the brain. GRE sequence effectively demonstrated hemosiderin deposition in the choroid plexus.

• 약학회지
• /
• 제50권4호
• /
• pp.278-286
• /
• 2006

Classical dihydropyrrole[3,4-f]quinazoline antifolates 7,8 and 9, in which the tricyclic ring is structurally similar to the pteridine ring of $CH_2-THF(1)$, the cofactor of thymidylate synthase (TS), were synthesized, and their in vitro antitumor activity was evaluated by measuring the cell growth inhibitory activity against cancer cell lines. The target compounds were cytotoxic against CCRF-CEM, human T-cell acute lymphoblastic leukemia, with the cell growth inhibitory activity $(IC_{50})$ of $0.8{\sim}8.3\;{\mu}M$. Among the three compounds, 3-amino analog 7 was 10- and 3.5-fold more cytotoxic compared to the 3-methyl analogs 8 and 9, and its cytotoxicity was similar to that of the reference compound with the $IC_{50}$ value of $0.83\;{\mu}M$. This result was supposed as the consequence of the fact that dihydropyrroloquinazolinone ring with amino group was able to bind well in the active site of TS. In the case of 3-methyl analogs, analog 9, which has two-carbon bridge between the dihydropyrroloquinazolinone ring and benzoyl-L-glutamic acid, was 3-times more potent in cytotoxicity than analog 8 which has one-carbon bridge, and this result indicates that the distance and conformational orientation of the benzoyl-L-glutamic acid moiety with respect to the tricyclic ring may also be a crucial determinant of cell growth inhibitory activity.

• 한국해양생명과학회지
• /
• 제7권2호
• /
• pp.121-128
• /
• 2022

L-asparaginase (ASNase) is a therapeutic enzyme used to treat acute lymphoblastic leukemia. Currently, the most widely used ASNases are originated from bacteria. However, owing to the adverse effects of bacterial ASNases, new resources for ASNase production should be explored. Fungal enzymes are considered efficient and compatible resources of natural products for diverse applications. In particular, fungal species belonging to the genus Trichoderma are well-known producers of several commercial enzymes including cellulase, chitinase, and xylanase. However, enzyme production by marine-derived Trichoderma spp. remains to be elucidated. While screening for extracellular ASNase-producing fungi from marine environments, we found four strains showing extracellular ASNase activity. Based on the morphological and phylogenetic analyses using sequences of translation elongation factor 1-alpha (tef1α), the Trichoderma isolates were identified as T. afroharzianum, T. asperellem, T. citrinoviride, and Trichoderma sp. 1. All four strains showed different ASNase activities depending on the carbon sources. T. asperellem MABIK FU00000795 showed the highest ASNase value with lactose as a carbon source. Based on our findings, we propose that marine-derived Trichoderma spp. are potential candidates for novel ASNase production.

• Tuberculosis and Respiratory Diseases
• /
• 제72권3호
• /
• pp.284-292
• /
• 2012

Background: The aim of this study was to investigate therapeutic outcomes and assess factors associated with therapeutic outcomes in hematologic patients with invasive pulmonary aspergillosis (IPA). Methods: We analyzed all consecutive cases of IPA in adults with hematologic diseases from January 2008 to January 2009 at a Catholic Hematopoietic Stem Cell Transplantation (HSCT) Center in Seoul, Korea. Results: A total of 54 patients were identified. Underlying diseases were acute myelogenous leukemia (n=25), acute lymphoblastic leukemia (n=10), myelodysplastic syndrome (n=7), chronic myelogenous leukemia (n=3), multiple myeloma (n=3), severe aplastic anemia (n=2) and other hematologic diseases (n=4). Twenty six patients (48.2%) were assessed as having a favorable response, of which 16 patients (29.6%) showed complete response. Overall 12-week mortality and IPA attributable mortality were 38.9% (n=21) and 33.3% (n=18), respectively. In multivariate analysis, uncontrolled underlying disease (odds ratio [OR], 7.31; 95% confidence interval [CI], 1.49~35.94; p=0.014) was associated with an unfavorable response, and for 12-week mortality, uncontrolled underlying disease (OR, 11.79; 95% CI, 1.49~93.46; p=0.020) and hypoalbuminemia (OR, 9.89; 95% CI, 1.42~68.99; p=0.021) were significantly poor prognostic factors. Conclusion: IPA still remains as a poor therapeutic outcome, especially in patients with refractory hematologic diseases.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권15호
• /
• pp.6463-6468
• /
• 2015

Background: Chemotherapy is one of the common approaches in treatment of cancers, especially leukemia. However, drug resistance phenomena reduce the likelihood of treatment success. Resveratrol is a herbal compound which through complicated processes makes some selected cells sensitive to treatment and induction of apoptosis. In the present study, the effects of resveratrol on the expression of miR 15a and miR16-1 and apoptosis in the CCRF-CEM cell line were investigated. Materials and Methods: The CCRF-CEM cell line was cultured under standard conditions and changes in miR 15a and miR 16-1 expression were analyzed by real time-PCR technique, with attention to reveratrol dose and time dependence. Also, apoptosis is evaluated by flow cytometry using annexin V and PI. Results: CCRF-CEM cells underwent dose-dependent apoptotic cell death in response to resveratrol. MiR 15a and miR 16-1 expression was up-regulated after 24 and 48 hours resveratrol treatment (p<0.05). Conclusions: The results of our study indicate that resveratrol induces apoptosis in a time and dose-dependent manner in CCRF-CEM cells. Also, increased expression level of miR 16-1 and miR 15a by means of resveratrol in CCRF-CEM cells might have a role in apoptosis induction and predisposition. According to our results resveratrol can be regarded as a dietary supplement to improve efficacy of anti-leukemia therapies.