• 제목/요약/키워드: low-oxygen

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Determination of Oxygen Transfer Coefficient in Fed-Batch Culture of Streptomyces avermitilis with Concentrated Medium Control (농축 배지 조절 유가식 배양에 의한 Streptomyces avermitilis의 산소전달계수 측정)

  • 오종현;전계택;정요섭
    • KSBB Journal
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    • v.16 no.5
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    • pp.516-522
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    • 2001
  • The large-scale production of antibiotics by filamentous mycelial organism requires and adequate supply of dissolved oxygen. In terms of productivity, it means that oxygen transfer is the rate-limiting step. Therefore, the oxygen transfer coefficients(K$\_$L/A) were determined in a broth involving a filamentous mycelial organism such as Streptomyces avermitilis for use in fermentations. To determine (K$\_$L/A) inn a stirred vessel, a great deal of effort is required to provide all the cells with a sufficient oxygen supply. To overcome the oxygen limitation in a batch culture, a fed-batch culture was applied to control the growth rate by an intermittent supply of nutrients. Thus, it was possible to maintain a suitable dissolved oxygen concentration at a low agitation rate. The optimal agitation speed was 350 rpm at low cell concentrations (below 7 g/L) by considering the efficiency of agitation and shear stress. The (K$\_$L/A) was found to decrease from 64.26 to 29.21h.$\^$-1/ when the biomass concentration was increased from 9.82 to 12.06 g/L. In addition, and increase in viscosity was also observed during the growth phase. By comparing the (K$\_$L/A) values for the various agitation and aeration rates, it was found that the effect of an increase in (K$\_$L/A) by aeration was reduced dramatically at high biomass concentrations. However, this effect was not observed when altering the agitation rate. This suggests that controlling the dissolved oxygen concentration by altering the agitation rate was more efficient than increase the aeration rate.

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Dynamic Boric Acid Corrosion of Low Alloy Steel for Reactor Pressure Vessel of PWR using Mockup Test (가압형 경수로 압력용기 재료인 저합금강의 동적 붕산 부식 실증 연구)

  • Kim, Sung-Woo;Kim, Hong-Pyo;Hwang, Seong-Sik
    • Corrosion Science and Technology
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    • v.12 no.2
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    • pp.85-92
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    • 2013
  • This work is concerned with an evaluation of dynamic boric acid corrosion (BAC) of low alloy steel for reactor pressure vessel of a pressurized water reactor (PWR). Mockup test method was newly established to investigate dynamic BAC of the low alloy steel under various conditions simulating a primary water leakage incident. The average corrosion rate was measured from the weight loss of the low alloy steel specimen, and the maximum corrosion rate was obtained by the surface profilometry after the mockup test. The corrosion rates increased with the rise of the leakage rate of the primary water containing boric acid, and the presence of oxygen dissolved in the primary water also accelerated the corrosion. From the specimen surface analysis, it was found that typical flow-accelerated corrosion and jet-impingement occurred under two-phase fluid of water droplet and steam environment. The maximum corrosion rate was determined as 5.97 mm/year at the leakage rate of 20 cc/min of the primary water with a saturated content of oxygen within the range of experimental condition of this work.

Study on Halogen Free Low Smoke Polyolefin (할로겐이 없는 저연성 폴리올레핀에 관한 연구)

  • Kim, Young-Doo;Chung, Kwang-Soo;Kim, Tae-Ho
    • Elastomers and Composites
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    • v.37 no.3
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    • pp.177-182
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    • 2002
  • For low-smoke-type polyolefin compound, LDPE, EVA, and EEA as base resin, magnesium trihydrate, alumina trihydrate, and red phosphorous as flame retardant and MAH type compatibilizer were applied. The amount of each component was changed to find out optimum composition. Mechanical properties were obtained by tensile test and residual stress after aging and flame retardancy was evaluated by smoke density, LOI(Limit Oxygen Index), and UL-94 test. SEM was used for the investigation or morphology and halogen contents were obtained by measuring the amount of HCI. Two kinds of halogen free compositions for flame retardant and low smote resin were found and it is expected to be applied for various purposes.

Study of the temperature container system for a live fish transportation (활어수송용 저온 컨테이너 시스템 연구)

  • 윤석만;김종보;조영제;허병기
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.10 no.3
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    • pp.343-347
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    • 1998
  • The objective of this study is to manufacture the low temperature waterless container that is compact and low cost for a live fish transportation. Using the low temperature water container, it makes observations on the optimal conditions such as the amount of dissolved oxygen, total ammonia and nitrite in seawater for determining the survival rate of live fish in short and long-term transportation. Using a sole as a live fish, the temperatures of $0^{\circ}C$, 3$^{\circ}C$, 5$^{\circ}C$, 7$^{\circ}C$, 15$^{\circ}C$ were controled for there effects. The results of this investigation show that as the seawater temperature increased, the amount of oxygen decreased and there was a low temperature shock below 3$^{\circ}C$. It was observed that the fish was died with 30$m\ell/\ell$of ammonia. The optimal temperature is about 5$^{\circ}C$ for live fish transportation to maintain best survival rate.

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Low Temperature Growth of Silicon Oxide Thin Film by In-direct Contacting Process with Photocatalytic TiO2 Layer on Fused Silica (광촉매 TiO2 층의 비접촉식 공정을 통한 저온 실리콘 산화박막 성장)

  • Ko, Cheon Kwang;Lee, Won Gyu
    • Applied Chemistry for Engineering
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    • v.19 no.2
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    • pp.236-241
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    • 2008
  • The possibility of silicon oxidation through the aerial-diffusion of active oxygen species has been evaluated. The species originate from the surface of $TiO_2$ exposed by UV. Among process parameters such as UV intensity, substrate temperature and chamber pressure with oxygen, UV intensity was a major parameter to the influence on the oxide growth rate. When 1 kW high pressure Hg lamp was used as a UV source, the growth rate of silicon oxide was 8 times as faster as that of a 60 W BLB lamp. However, as the chamber pressure increased, the growth rate was declined due to the suppression of aerial diffusion of active oxygen species. According to the results, it could be confirmed that the aerial-diffusion of active oxygen species from UV-irradiated photocatalytic surface can be applied to a new method for preparing an ultra-thin silicon oxide at the range of relatively low temperature.

State of the Art in the Development of Methane/Oxygen Liquid-bipropellant Rocket Engine (메탄/산소 이원액체추진제 로켓엔진 기술개발 동향)

  • Kim, Jeong Soo;Jung, Hun;Kim, Jong Hyun
    • Journal of the Korean Society of Propulsion Engineers
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    • v.17 no.6
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    • pp.120-130
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    • 2013
  • A study was conducted for the performance characteristics of methane taking recently the limelight in the world as a next-generation propellant, with the survey for state of the art in the development of methane/oxygen rocket engine being accompanied. Liquid methane as a rocket fuel has the favorable characteristics such as non-toxic, low cost, regenerative cooling capability, and potential for in-situ resource utilization (ISRU). The combination of liquid methane and liquid oxygen also provides the excellent performance including high specific impulse and low system mass. For these reasons, many researches have been actively carried out on the methane/oxygen engine, nevertheless, its technology readiness level is not that high enough just yet. Therefore, it is judged that it is the time to mitigate the technical gap with the space technology of advanced countries through a swift onset of the development of methane rocket engine.

Estimation of Dissolved Oxygen in Streams using Reaeration, 1st Production and Respiration Rates (재포기 계수, 1차 생산율 및 호흡률을 이용한 하천의 용존산소 추정)

  • Kim, Kyung-Sub;Hwang, Sung-Gyu
    • Journal of Korean Society of Environmental Engineers
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    • v.31 no.6
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    • pp.428-433
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    • 2009
  • Dissolved oxygen is considered as one of the important water-quality constituents in streams from one century ago and fishes perish in low dissolved oxygen concentration. Environmental scientists and engineers have introduced the deterministic model to estimate dissolved oxygen concentration of streams and recommended the use of the Delta Method (DM), Approximate Delta Method (ADM), Extreme Value Method (EVM) and Optimization Method (OPT) which can be applied in no spatial variation of dissolved oxygen. The diurnal or annual variation of dissolved oxygen is mainly determined from the parameters such as reaeration rate, 1st production rate and respiration rate which are related to dissolved oxygen. Each method was briefly introduced and applied to two sampling sites of Anseong Stream watershed in this paper. The limitation, advantages and disadvantages of each method were reviewed and analyzed after running the each method. From these analyses, the benefit-cost approach to estimate dissolved oxygen effectively in streams was recommended.

Correlation between Oxygen Related Bonds and Defects Formation in ZnO Thin Films by Using X-ray Diffraction and X-ray Photoelectron Spectroscopy (XRD와 XPS를 사용한 산화아연 박막의 결함형성과 산소연관 결합사이의 상관성)

  • Oh, Teresa
    • Korean Journal of Materials Research
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    • v.23 no.10
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    • pp.580-585
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    • 2013
  • To observe the formation of defects at the interface between an oxide semiconductor and $SiO_2$, ZnO was prepared on $SiO_2$ with various oxygen gas flow rates by RF magnetron sputtering deposition. The crystallinity of ZnO depends on the characteristic of the surface of the substrate. The crystallinity of ZnO on a Si wafer increased due to the activation of ionic interactions after an annealing process, whereas that of ZnO on $SiO_2$ changed due to the various types of defects which had formed as a result of the deposition conditions and the annealing process. To observe the chemical shift to understand of defect deformations at the interface between the ZnO and $SiO_2$, the O 1s electron spectra were convoluted into three sub-peaks by a Gaussian fitting. The O 1s electron spectra consisted of three peaks as metal oxygen (at 530.5 eV), $O^{2-}$ ions in an oxygen-deficient region (at 531.66 eV) and OH bonding (at 532.5 eV). In view of the crystallinity from the peak (103) in the XRD pattern, the metal oxygen increased with a decrease in the crystallinity. However, the low FWHM (full width at half maximum) at the (103) plane caused by the high crystallinity depended on the increment of the oxygen vacancies at 531.66 eV due to the generation of $O^{2-}$ ions in the oxygen-deficient region formed by thermal activation energy.