• Title/Summary/Keyword: low-order control

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Protective Effect of Flavonoids on Lymphocyte DNA Damage Using Comet Assay (Comet Assay를 이용한 Flavonoids와 항산화 비타민의 인체임파구 세포 DNA 손상 보호 효과)

  • 박유경;전은재;강명희
    • Journal of Nutrition and Health
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    • v.36 no.2
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    • pp.125-132
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    • 2003
  • The present study was attempted to investigate and compare the antioxidant potency of several well-know flavonoids, antioxidant vitamin and commercially available popular beverages. The antioxidant potency was assessed by the effect on reducing oxidative DNA damage of human lymphocytes. Cellular oxidative DNA damage was measured by SCGE (single-cell gel electrophoresis), also known as comet assay. Lymphocytes were pre-treated for 30 minutes with wide ranges of doses of apigenin, kaempferol, luteolin, myricetin, rutin, quercetin, $\alpha$-tocopherol (10,25,50,100,200,500,1000 $\mu$M) ,green tea extract or grape juice (10,50,100,250,500,1000 $\mu$g/mL) followed by a $H_2O$$_2$(100 $\mu$M) treatment for 5 min as an oxidative stimulus. The physiological function of each antioxidant substance on oxidative DNA damage was analyzed as tail moment (tail length $\times$ percentage migrated DNA in tail) and expressed as relative DNA damage score after adjusting by the level of control treatment. Cells treated with $H_2O$$_2$alone (positive control) had an extensive DNA damage compared with cells treated with phosphate buffered saline (PBS, negative control) or pre-treated with all the tested samples. Of all the six flavonoids, quercetin was the most potent antioxidant showing the lowest $ED_{50}$/ of 8.5 $\mu$g/mL (concentration to produce 50% protection of relative DNA damage). The antoxidant potency of individual flavonoids were ranked as follows in a decreasing order; luteolin (18.4 $\mu$g/mL), myricetin (19.0 $\mu$g/mL) , rutin (22.2 $\mu$g/mL) , apigenin (24,3 $\mu$g/mL) , kaempferol (25.5 $\mu$g/mL). The protective effect of $\alpha$-tocopherol was substantially lower (highest $ED_{50}$value of 55.0 $\mu$g/mL) than all the other flavonoids, while the protective effect was highest in green tea and grape juice with low ED5O value of 7.6 and 5.3, respectively. These results suggest that flavonoids, especially quercetin, and natural compounds from food product, green tea and grape juice, produced powerful anti-oxidative activities, even stronger than $\alpha$-tocopherol. Taken together, supplementation of antioxidants to lymphocytes followed by oxidative stimulus inhibited damage to cellular DNA, supporting a protective effect against oxidative damage induced by reactive oxygen species.

Study on the energy-saving constant temperature and humidity machine operating characteristics (에너지 절감형 항온항습기 운전 특성에 관한 연구)

  • Cha, Insu;Ha, Minho;Jung, Gyeonghwan
    • Journal of Energy Engineering
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    • v.25 no.3
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    • pp.27-33
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    • 2016
  • The heat recovery system that was applied in this study, is the energy-saving type that can produce the maximum cooling capacity less power in use. In order to have a more precise control function the temperature and humidity of the constant temperature and humidity machine, control algorithm is applied to designed a fuzzy PID controller, and the outside air compensation device (air-cooled) demonstrated excellent ability to dehumidify the moisture, $-20^{\circ}C$ in winter. High efficiency and the low-noise type sirocco fan operate quitely and designed to fit the bottom-up and top-down in accordance with the characteristics of equipment. as a result of experiment data, the conversion efficiency is 95% or more, power recovery time is within 5sec, stop delay time is within 30sec, pump down time is 10sec, pump delay time is 5sec, heating delay time is 5sec, temperature deviation is ${\pm}2^{\circ}C$ (cooling deviation: $2^{\circ}C$, Heating deviation : $2^{\circ}C$), humidity deviation is a ${\pm}5%$ (humidification deviation 3.0%, dehumidification deviation 3.0%). Recently, ubiquitous technology is important. so, the constant temperature and humidity machine designed to be able to remotely control to via the mobile phone, and more scalable to support MMI software and automatic interface. Further, the life of the parts and equipment is extended by the failure.

Improving the Protection and Security System Outside the National Assembly Building (국회 외곽 경호·경비시스템 발전방향에 관한 연구)

  • Choi, O-Ho
    • Korean Security Journal
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    • no.60
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    • pp.113-135
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    • 2019
  • Despite being one of the most important national facilities, the National Assembly building of the Republic of Korea has become increasingly vulnerable to potential terrorist attacks, and the instances of demonstrations occurring around and banned items taken into the building are continuing to rise. In addition, promoting the idea of "open assembly" has led to increased visitors and weakened access control. Furthermore, while there is a significant symbolic value attached to attacking the National Assembly building, the level of security management is relatively very low, making it a suitable target for terrorism. In order to address such vulnerability, an appropriate access control system should be in place from the areas surrounding the building. However, the National Assembly Security Service which oversees security around the building is scheduled to disband in June 2020 following the abolition of the conscripted police force in 2023. Therefore, there needs to be an alternative option to bolster the security system outside the facility. In this research, the perceptions of 114 government officials in charge of security at the National Assembly Secretariat toward the protection and security system of the areas surrounding the National Assembly building were examined. Results showed that the respondents believed it was highly likely that risky situations could occur outside the building, and the use of advanced technologies such as intelligent video surveillance, intrusion detection system, and drones was viewed favorably. Moreover, a mid- to long-term plan of establishing a unified three-layer protection system and designating a department in charge of the security outside the building were perceived positively. Lastly, the participants supported the idea of employing private police to replace the National Assembly Security Service for the short term and introducing parliamentary police for the mid- to long-term.

Selection of Appropriate Herbicides for Establishment of Weed Control System in Adzukibean and Mungbean (팥과 녹두 잡초방제체계(雜草防除體系) 수립(樹立)을 위한 제초제(除草劑) 병발(迸拔)에 관한 연구)

  • Hong, E.H.;Lee, Y.H.;Kim, S.D.;Hwang, Y.H.;Moon, Y.H.
    • Korean Journal of Weed Science
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    • v.3 no.2
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    • pp.199-207
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    • 1983
  • To select appropriate herbicides for adzukibean and mungbean, a series of experiments was carried out in both field and pot from 1980 to 1983. Tolerance to the herbicides tested was highest in soybeans and followed by mungbean and adzukibean in the order. Pre-emergence herbicides showed relatively low phytotoxicity were chlorambem, linuron, and metribuzin for adzukibean and alachlor and butachlor for mungbean. Post-emergence herbicides showed no external phytotoxicity for adzukibean and mungbean were alloxydim, Dowco 453, fluazifop, etc.. For mungbean, terbutryn (1.5 kg, a.i./ha) which is pre-emergence herbicide showed the best weeding control efficacy but some possible phytotoxicity. Among post-emergence herbicides, acifluorfen (300 g, a.i./ha) showed the best weeding efficacy with no yield reduction though some phytotoxicity which recovered within 20 days. Compared to single herbicide application, the mixture or systemic treatments of herbicides showed much higher weeding control efficacy and seed yields: the systemic treatments of linuron (500 g, a.i./ha) / Dowco 453 (180 g, a.i./ha) or linuron (500 g, a.i./ha) / fluazufop (260 g, a.i./ha) for adzukibean and the mixture treatment of alachlor (1.31 kg, a.i./ha) / acifluorfen (150 g, a.i./ha) for mungbean, respectively.

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Physical and Mechanical Properties of Heat-treated Domestic Yellow Poplar (백합나무 열처리재의 물리 및 역학적 특성)

  • Kim, Kwang-Mo;Park, Jung-Hwan;Park, Byoung-Soo;Son, Dong-Won;Park, Joo-Saeng;Kim, Wun-Sub;Kim, Byoung-Nam;Shim, Sang-Ro
    • Journal of the Korean Wood Science and Technology
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    • v.38 no.1
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    • pp.17-26
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    • 2010
  • Recently, yellow poplar (Liriodendron tulipifera L.) is getting attention in Korea due to the fast growing and high yield and quality of lumber. But, it is thought that the color difference between heartwood and sapwood may restrict the practical use of it. This study was aimed to enhance the value of yellow poplar lumber by the color control using high temperature heat-treatment, which had been tried for domestic cedar (Kim et al., 2009). The material properties including surface color of yellow poplar lumber were evaluated according to heat treatment conditions. The difference of color between sapwood and heartwood could be reduced by heat treatment at a temperature about $200^{\circ}C$. Long heating time was more effective in reducing the difference. The Equilibrium Moisture Content (EMC) of heat-treated wood was as low as 50 percent of the control. The result obviously indicates that heat-treated wood is more dimensionally stable in the change of moisture condition. The durability against wood rotting fungi also increased by the heat-treated, but it was not so effective as the case of cedar. The changes of mechanical properties of heat-treated yellow poplar were very similar to that of heat-treated cedar. In order to develop new use of heat-treated yellow poplar, the changes of mechanical properties should be considered. There were no significant changes in microscopic structure which may cause changes in mechanical properties. Further study of heat-treated wood is needed to scrutinize the causes of changes of material properties.

The Evaluation of Metabolizable Energy of Angelica Keiskei (Angelica utilis Makino) Products (명일엽 가공산물의 대사 에너지 함량 평가)

  • Kim, Eun-Mi;Choi, Jin-Ho;Choi, Kum-Boo;Yeo, Ik-Hyun
    • Journal of Nutrition and Health
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    • v.43 no.1
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    • pp.5-11
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    • 2010
  • We conducted comparative study on metabolizable energy content of extracts of angelica keiskei and its byproduct. Total six different groups consisting of five test groups treated with angelica keiskei and one control group were compared. Each of the five test groups were given 30% of one of whole plant, extracts, fermented of extracts, byproduct and extracts plus byproduct, respectively, mixed with AIN93M. After 3 days of adjustment period, all groups were subjected to 4 days of test period during which the amounts of feed intake and excretion were measured everyday. All feces were treated for the prevention of decomposition and changes before its energy content were measured using a bomb calorimeter. The amount of excretion was $4.8\;{\pm}\;0.3\;g$/rat/3 days in control group and 9.9-15.0 g/rat/3 days in the groups were added with extracts of angelica keiskei indicating that the angelica keiskei-treated groups produce 2-3 times more excretion. Metabolic energy of control diet was 4,133.3 kcal. This was found to be 15 to 20% higher compared with the metabolic energy content ranging from 3,117.0 kcal/kg (extracts of angelica keiskei) to 3,259.8 kcal/kg (extracts plus byproducts) angelica keiskei-treated groups. This is interpreted as the result of the decreased metabolic energy in the test diets were substituted with 30% of ngelica keiskei-treated ingredient which has low metabolic content itself. One notable finding is that the metabolic content of the group mixed with byproducts and extracts (1,763.0 kcal/kg) is 27% higher than that of extracts of angelica keiskei (1,286.8 kcal/kg) indicating that mechanical grinding increases the rate of digestion and absorption increasing, in turn, the energy content used in the body. The results of analysis of overall caloric absorption showed absorption rate in order of Whole plant < extracts < byproduct < extract plus byproduct < fermented of extract.

A Priority and Impact Factor Analysis of Construction Management Tasks for Decreasing Change Orders and Defect Repairs in Cancer Treatment Center Projects (암센터 의료시설의 설계변경과 하자보수의 감소를 위한 중점 관리공사 분석)

  • Lee, Chijoo;Lee, Ghang;Sim, Jaekyang
    • Korean Journal of Construction Engineering and Management
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    • v.14 no.5
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    • pp.55-64
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    • 2013
  • The complexity of constructing medical institutions is higher than that of general buildings, and many change orders in the design and defect repairs in the construction phase are required due to strict government regulations. The priority control of constructions and impact factors of medical institutions were analyzed in this study, and difficulties in the control in the design and construction phase were identified. First, the priority management factors that were identified were as follows: architecture, facilities, and electricity. Second, 1) priority management in constructions and factors resulting in change orders and 2) priority management in constructions involving defect repair were analyzed. Third, the importance recognized by the construction managers were analyzed. The priority management in constructions and factors that were recognized by the construction manager were deducted as having low importance, although there were many change orders and defects. The work of finishing, wall building, joining, office automation and communication function, and lighting were analyzed in the design phase, and waste, the office automation and communication function, ceilings, contamination control, and plumbing were analyzed in the construction phase. The results showed that there will be a decrease in change orders and defects if the concentration of the manager was elevated and priorities were managed.

A SCANNING ELECTRON MICROSCOPIC STUDY ON THE MARGINAL ADAPTIBILITY IN APPLYING THE CAVITY VARNISH AND DENTIN BONDING AGENT IN AMALGAM RESTORATIONS (아말감 수복시(修復時) Cavity varnish와 Bonding agent 도포(塗布)에 따른 접합성(接合性)에 관(關)한 주사전자현미경적(走査電子顯微鏡的) 연구(硏究))

  • Kim, Seok-Hoon;Cho, Young-Gon
    • Restorative Dentistry and Endodontics
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    • v.15 no.1
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    • pp.107-119
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    • 1990
  • The purpose of this study was to evaluate the marginal adaptability of the amalgam restorations in applying the cavity varnish (Copalite$^{(R)}$) and dentin bonding agent (Scotchbond 2$^{(R)}$) under the scanning electron microscope. For this study, eighteen sound extracted human molars were selected. Class I cavities in 12 teeth and class V cavities in 6 teeth were prepared using an air turbine with No. 701 tungsten carbide bur and finished using a low speed handpiece with No. 557 fissure bur. The prepared specimens were then divided into three groups including 4 class I cavities and 2 class V cavities in each group and restored as follows ; Group I. All the prepared cavities were restored with amalgam only (Control). Group II. Two layers of Copalite$^{(R)}$ cavity varnish were applied to the cavities with a gentle stream of air after each application and cavities were restored with amalgam. Group III. The enamel cavity margins were etched with 37% phosphoric acid gel for 60 sec., rinsed for 30 sec. and dried. One layer of visible lightcured Scotchbond Dental Adhesive$^{(R)}$ was applied and immediately cured for 20 seconds with visible light-cure unit and cavities were restored with amalgam. All the specimens were cut at the neck of the teeth and the occlusal halves of specimens were sectioned buccolingually in the longitudinal axis centering the amalgam restorations, using the disk. The cut specimens were ground with sandpapers (400, 600, 800, 1000 grit), and cleaned for 5 minutes in the ultrasonic cleaner (Brason Co. U.S.A.). In the cut surfaces, the amalgam - tooth interfaces were examined under the scanning electron microscope (JSM, 35C type, JEOL). The obtained results were as follows ; 1. The amalgam-tooth interfaces were reduced more significantly in the Copalite$^{(R)}$ and Scotchbond 2$^{(R)}$ application group than in the control group. 2. In the class I cavities, the Scotchbond 2$^{(R)}$ application group showed the findings similar to the Copalite$^{(R)}$ application group in the cavity floor, and the marginal adaptability was better in the side wall than in the cavity floor. 3. In the class I cavities, the Scotchbond 2$^{(R)}$ application group showed better marginal adaptability in the occlusal margin than in the gingival margin. 4. The marginal adaptability was in the order of the Scothbond 2$^{(R)}$ application group, the Copalite$^{(R)}$ application group and the control group.

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Effect of Bone Marrow Aspirate with Autogenous Bone graft for Alveolar Cleft in a new Rabbit Model (가토의 치조열 모델에서 골수 흡인물이 자가뼈 이식술에 미치는 효과)

  • Bae, Sung Gun;Chung, Ho Yun;Lee, Sang Yun;Cho, Byoung Chae;Yang, Jung Dug;Park, Mee Young
    • Archives of Plastic Surgery
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    • v.36 no.5
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    • pp.531-537
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    • 2009
  • Purpose: Alveolar bone grafting has become an essential process in the treatmemt of alveolar cleft patient for stabilization of the maxillary arch, elimination of oronasal fistula, the reconstruction of the soft tissue nasal base support, and creation of bony support for tooth eruption for implant. The use of Autologous iliac cancellous bone is preferable because of the adequate quantity and high osteoinductive potential. However, even with iliac bone, insufficient osteoregeneration and absorption occur due to several factors such as the patient's age, cleft width, functional stress, and others. In order to increase osteoregeneration where the iliac bone is placed, the present study is associated with bone marrow aspirate (BMA). The experimental study evaluated the efficacy of osteoregeneration in normal cleft rabbits when alveolar bone grafting was performed with autologous iliac corticocancellous bone. Methods: Twenty - four New Zealand White rabbits were divided randomly into 2 groups (BMA, control). All animals underwent harvesting of corticocancellous bone graft from the right posterior iliac crest via standard surgical technique. $1m{\ell}$ of BMA were obtained by scraping the needle and aspirate with $10m{\ell}$ syringe from the contralateral iliac bone wall. The muco - periosteal flap on the palate was elevated. A mixture of Equal bone's volumes with BMA and saline as its control was inserted into the cleft. Animals were sacrificed at 2, 4, and 8 weeks and maxilla was harvested for dental peri - apical X-ray, bone matrix density (BMD),and histologic analysis. Result: BMD of regenerated bone to the cleft in the rabbits was higher than that of the control rabbits. X-ray, histologic analysis showed that increased osteoregeneration and low absorption rate were observed in the BMA group. Conclusion: Our experimental study showed BMA enhanced the osteoregeneration and survival rate of alveolar bone grafting. BMA is easy to extract & cost - time effective. So it can be an effective enhancers for bone grafting mixtures.

Growth Characteristics of Purple Nutsedge(Cyperus rotundus L.) and Establishment of Its Effective Control Method (향부자(Cyperus rotundus L.)의 생육특성 및 방제법에 관한 연구)

  • Kim, Kyoung-Im;Kim, Kil-Ung;Shin, Dong-Hyun;Lee, In-Jung
    • Korean Journal of Weed Science
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    • v.18 no.2
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    • pp.136-145
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    • 1998
  • This study was conducted to determine the growth characteristics, and the effect of plant growth regulators on the sprouting and growth of purple nutsedge(Cyperus rotundus L.) in order to establish effective control system in lawn ground. The flowering of purple nutsedge was initiated 30 days after transplanting regardless of the transplanting time. Low temperature less than $10^{\circ}C$ after flowering was required for tuber formation, showing that the tuberization was related to air temperature. Shoot number and dry weight of underground portion of purple nutsedge was slightly affected by plant growth regulators such as benzylamino purine, abscisic acid, brassinosteroid and jasmonate. Imazaquin applied at 1, 2 and 3 weeks after transplanting induced multi-shooting and inhibited shoot growth indicating that the herbicide played a role as plant growth regulator at a concentration of 30 and 60g ai/10a. The greatest inhibition of purple nutsedge was obtained by pyrazosulfuron-ethyl as applied 1 weeks after transplanting, showing almost 100% control of purple nutsedge. Tuber of purple nutsedge composed of 61.83% of moisture, 31.60% of carbohydrates, 4.03% of crude protein, 1.57% of crude fat and 0.97% of crude ash.

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