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Phylogenetic and expression analysis of the angiopoietin-like gene family and their role in lipid metabolism in pigs

  • Zibin Zheng;Wentao Lyu;Qihua Hong;Hua Yang;Ying Li;Shengjun Zhao;Ying Ren;Yingping Xiao
    • Animal Bioscience
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    • v.36 no.10
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    • pp.1517-1529
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    • 2023
  • Objective: The objective of this study was to investigate the phylogenetic and expression analysis of the angiopoietin-like (ANGPTL) gene family and their role in lipid metabolism in pigs. Methods: In this study, the amino acid sequence analysis, phylogenetic analysis, and chromosome adjacent gene analysis were performed to identify the ANGPTL gene family in pigs. According to the body weight data from 60 Jinhua pigs, different tissues of 6 pigs with average body weight were used to determine the expression profile of ANGPTL1-8. The ileum, subcutaneous fat, and liver of 8 pigs with distinct fatness were selected to analyze the gene expression of ANGPTL3, ANGPTL4, and ANGPTL8. Results: The sequence length of ANGPTLs in pigs was between 1,186 and 1,991 bp, and the pig ANGPTL family members shared common features with human homologous genes, including the high similarity of the amino acid sequence and chromosome flanking genes. Amino acid sequence analysis showed that ANGPTL1-7 had a highly conserved domain except for ANGPTL8. Phylogenetic analysis showed that each ANGPTL homologous gene shared a common origin. Quantitative reverse-transcription polymerase chain reaction analysis showed that ANGPTL family members had different expression patterns in different tissues. ANGPTL3 and ANGPTL8 were mainly expressed in the liver, while ANGPTL4 was expressed in many other tissues, such as the intestine and subcutaneous fat. The expression levels of ANGPTL3 in the liver and ANGPTL4 in the liver, intestine and subcutaneous fat of Jinhua pigs with low propensity for adipogenesis were significantly higher than those of high propensity for adipogenesis. Conclusion: These results increase our knowledge about the biological role of the ANGPTL family in this important economic species, it will also help to better understand the role of ANGPTL3, ANGPTL4, and ANGPTL8 in lipid metabolism of pigs, and provide innovative ideas for developing strategies to improve meat quality of pigs.

Identifying subgroups of ageism among young adults, and its relationship to perceptions of generational conflict and elderly welfare policy (청년세대의 연령주의 유형화 및 연령주의 유형과 세대갈등·노인복지정책 인식의 관계)

  • Lee, Sunhee;Kim, Miri;Chung, Soondool
    • 한국노년학
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    • v.39 no.4
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    • pp.825-846
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    • 2019
  • This study aims to identify subgroups of ageism among young adults of age 20-39, and to examine its relationship to perceptions of generational conflict and elderly welfare policy. Latent Profile Analysis(LPA) was applied on the data 'Survey on Age Integration and Generational Integration', which was executed by institute for Age Integration Researchof Ewha Womans university, and descriptive statistics and cross-tabulation analysis were performed to examine the socio-demographic characteristics of each subgroup. Then, regression analysis was performed to observe the effect of the subgroups on the perceptions of generational conflict and elderly welfare policy. The results are as follows. The resulting subgroups of ageism among young adults were 'compound perception on aging and active age discrimination', 'medium-level aging anxiety and passive age discrimination', and 'low-level aging anxiety and beyond age discrimination'. Subgroups of ageism affected both perceptions of generational conflict and elderly welfare policy, whereas social support only affected perception of elderly welfare policy. Based on the results, political implications, such as activation of education on perception on aging, expansion of generational exchange for age integration, and renewal of social atmosphere for intergenerational coexistence, which will promote social integration

Derivation of Representative Emotions Through Analysis of Perceived Frequency Profiles of Various Emotions According to Levels of Cognitive Well-Being (인지적 안녕감 수준에 따른 다양한 감정의 지각된 빈도 프로파일 분석을 통한 대표 감정 도출)

  • Dahye Han;Guk-Hee Lee
    • Science of Emotion and Sensibility
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    • v.26 no.3
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    • pp.83-100
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    • 2023
  • This study determines whether the perception of the frequency of experiencing positive, negative, and surprise emotions changes according to the level of cognitive well-being. Furthermore, we determined practical means to analyze which emotions can be managed in daily life as an effective means of improving overall life satisfaction by identifying representative specific emotions that strongly predict the level of cognitive well-being. To this end, the between-subjects factorial design is adopted to measure the frequency of emotional experiences according to the level of cognitive well-being in 438 university undergraduate students. For cognitive well-being, the life satisfaction scale (SWLS) was used, and the PANAS-X scale was used to measure emotional frequency. As a result, first, the group with high cognitive well-being displays a higher frequency of positive and surprise emotional experiences and a lower frequency of negative emotional experiences than the group with low cognitive well-being. Second, the results confirm that representative emotions affecting cognitive well-being included 8 positive emotions, 7 negative emotions, and 1 surprise emotion. Among them, positive emotions include "happy" and "confident," negative emotions include "dissatisfied with self" and "disgusted with self," and surprise emotions include words such as "amazed." Therefore, we can conclude that the representative emotions are those with the greatest influence on cognitive well-being. Therefore, increasing the frequency of specific emotions (e.g., happy, confident, and surprise) and decreasing the frequency of others (e.g., dissatisfied with self and disgusted with self) could be effective in improving cognitive well-being than unconditionally examining emotions experienced in daily life.

Large eddy simulation of a steady hydraulic jump at Fr = 7.3 (Fr = 7.3의 정상도수 큰와모의)

  • Paik, Joongcheol;Kim, Byungjoo
    • Journal of Korea Water Resources Association
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    • v.56 no.spc1
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    • pp.1049-1058
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    • 2023
  • The flow passing through river-crossing structures such as weirs and low-fall dams is dominated by rapidly varied flow including hydraulic jump. The intense unsteadiness of flow velocity and free surface profile affects the stability of such hydraulic structures. In particular, the steady hydraulic jump generated at high Froude number conditions includes remarkably air entrainment, making the flow characteristics more complicated. In this study, a large-eddy simulation was performed for turbulence effect and the hybrid VoF technique to simulate the steady hydraulic jump at the Froude number of 7.3 and the Reynolds number of 15,700. The results of the numerical simulation showed that the instantaneous maximum pressure and time-average pressure distribution calculated on the bottom surface downstream of the structure could be reasonably well reproduced being in good agreement with the experimental values. However, the instantaneous minimum pressure distribution in the direct downstream of the structure shows the opposite pattern to the target experimental measurement value. However, the numerical simulation performed in this study is considered to reasonably predict the minimum pressure distributions observed in various experiments conducted at similar conditions. The vertical distributions of flow velocity and air concentration computed in the center of the hydraulic jump were found to be in good agreement with the experimental results measured under similar conditions, showing self-similarity. These results show that the large eddy simulation and hybrid VoF techniques applied in this study can reproduce the hydraulic jump with strong air entrainment and the resulting intense free surface and pressure fluctuations at high Froude number conditions.

MT Response of a Small Island Model with Deep Sea and Topography (깊은 바다와 지형을 고려한 소규모 섬 모델의 MT 반응 연구)

  • Kiyeon Kim;Seong Kon Lee;Seokhoon Oh;Chang Woo Kwon
    • Geophysics and Geophysical Exploration
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    • v.27 no.1
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    • pp.37-50
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    • 2024
  • The magnetotelluric (MT) survey can be affected by external environmental factors. In particular, when acquiring MT data in islands, it is essential to consider the combined effect of topography and sea to understand the results and make accurate interpretations. To analyze the MT response (apparent resistivity, phase) with consideration of the effect of topography and sea, a small cone-shaped island model surrounded by deep sea was created. Two-dimensional (2-D) and three-dimensional (3-D) forward modeling were performed on the terrain model considering topography and the island model considering both topography and sea. The 2-D MT response did not reflect the topographic and sea effect of the direction orthogonal to the 2-D profile. The 3-D MT response included topographic and sea effects in all directions. The XY and YX components of the apparent resistivity were separated on undulating topography, such as a hill. A conductor at 1 km below sea level could be distinguished from topographic and sea effects in the MT response, and low resistivity anomaly was attenuated at greater depths. This study will facilitate understanding of field data measured on small islands.

A Study on the Structure Characteristics of Planting Ground in Incheon International Airport, Korea (인천국제공항 식재기반 구조 및 토양특성 연구)

  • Lee, Seung-Won;Han, Bong-Ho;Lee, Kyong-Jae;Kwak, Jeong-In;Yeum, Jung-Hun
    • Journal of the Korean Institute of Landscape Architecture
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    • v.43 no.3
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    • pp.77-91
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    • 2015
  • This study aims to suggest adequate soil management through the analysis of physicochemical properties of soil in the planting grounds of Incheon International Airport, which was constructed on a massive land reclamation site. Study areas were 5 sites at the international business complex, the passenger terminal, the airport support complex, the free trade zone, and the access road. Soil profile analysis showed that 9 plots out of the 27 plots were hardpan and heterospere within 80cm from the soil surface. The earth laid on the ground was categorized as gravel based soil(4 plots), dredged soil from the sea bottom and mixed reclamation materials(2 plots), clay with poor permeability(3 plots) and waste construction material(1 plot). Average soil hardness was $11.5kg/cm^2$ and soil textures were sandy soil, sandy loam and loamy sand. Average soil pH was 6.7 and average organic matter content was 0.7%. Electrical conductivity was 0.0dS/m and exchangeable cation concentrations were $Ca^{2+}$ 3.4cmol/kg, $Mg^{2+}$ 1.5cmol/kg, $K^+$ 0.3cmol/kg and $Na^+$ 1.0cmol/kg. Average cation exchange capacity was 11.0cmol/kg. Although average figures in Solum mostly meet the landscape design criteria, properties of each soil layer showed various values sometimes over the limit. Base saturations were $Ca^{2+}$ 29.9%, $Mg^{2+}$ 13.3% and $K^+$ 3.7% for lower soil, $Ca^{2+}$ 33.3%, $Mg^{2+}$ 17.0% and $K^+$ 2.7% for mid-soil and $Ca^{2+}$ 32.6%, $Mg^{2+}$ 12.2% and $K^+$ 1.9% for upper soil. Exchangeable sodium percentages were 16.4% for lower soil, 7.5% for mid-soil and 4.7% upper soil. Sodium adsorption rates were 0.8 for lower soil, 0.3 for mid-soil and 0.2 for upper soil. Factors affecting to the vegetation growth were heterogeneity and poorness of solum, disturbance of dredged soils, high soil hardness including hardpan in the subsurface soil layer and shallow effective soil depth, high soil acidity, imbalance of base contents, low organic matter content and low available phosphate levels in the soil.

Effects of NaCl Concentration on Physicochemical Properties of Pork Emulsion (NaCl 첨가량에 따른 돈육 유화물의 이화학적 특성)

  • Park, Sin-Young;Kim, Hack-Youn
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.4
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    • pp.551-556
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    • 2016
  • This study was conducted to investigate the effects of NaCl concentration on the physicochemical properties of pork emulsion. Pork emulsion was produced containing 0% (control), 0.3%, 0.6%, 0.9%, 1.2%, and 1.5% NaCl. Proximate composition of pork emulsion containing 1.5% NaCl showed the highest moisture content (P<0.05). The ash contents of pork emulsion increased with an increase in NaCl, and protein contents decreased with increasing NaCl concentration. The pH levels of uncooked pork emulsion containing 0.9%, 1.2%, and 1.5% NaCl were lower than those of other treatments (P<0.05), and the pH level of cooked pork emulsion containing NaCl was lower than that of the control (P<0.05). The CIE $L^*$ value of the uncooked pork emulsion samples containing NaCl was higher than that of the control (P<0.05), whereas CIE $a^*$ and CIE $b^*$ values of samples with NaCl were lower than the control (P<0.05). CIE $L^*$ and CIE $b^*$ values of cooked pork emulsion decreased with an increase in NaCl level, and CIE $a^*$ value increased with increasing NaCl concentration (P<0.05). Viscosity of the pork emulsion increased with an increase in NaCl. Texture profile analysis of pork emulsion containing NaCl showed no significant difference in springiness or cohesiveness (P>0.05). Pork emulsion containing 1.5% NaCl showed the highest hardness, gumminess, and chewiness (P<0.05). These results suggest that pork emulsion containing 0.9% and 1.2% NaCl can be used as a low-salt meat product.

A Study on Development of Prototype Test Train Design in G7 Project for High Speed Railway Technology (G7 고속전철기술개발사업에서의 시제차량 통합 디자인 개발)

  • 정경렬;이병종;윤세균
    • Archives of design research
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    • v.16 no.4
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    • pp.185-196
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    • 2003
  • The demand for an environment-friendly transportation system, equipped with low energy consumption, and low-or zero-pollution has been on the increase since the beginning of the World Trade Organization era. Simultaneously, the consistent growth of high-speed tram technology, combined with market share, has sparked a fierce competition among technologically-advanced countries like France, Germany, and Japan in an effort to keep the lead in high-speed train technology via extensive Research and development(R&D) expenses. These countries are leaders in the race to implement the next-generation transportation system, build intercontinental rail way networks and export the high-speed train as a major industry commodity. The need to develop our own(Korean) 'high-speed train' technology and its core system technology layouts including original technology serves a few objectives: They boost the national competitive edge; they develop an environmental friendly rail road system that can cope with globalization and minimize the social and economic losses created by the growing traffic-congested delivery costs, environment pollution, and public discomforts. In turn, the 'G7 Project-Development of High Speed Railway Technology' held between 1996 and 2002 for a six-year period was focused on designing a domestic train capable of traveling at a speed of 350km/h combined and led to the actual implementation of engineering and producing the '2000 high-speed train:' This paper summarizes and introduces one of the G7 Projects-specifically, the design segment achievement within the development of train system engineering technology. It is true that the design aspect of the Korean domestic railway system program as a whole was lacking when compared with the advanced railroad countries whose early phase of train design emphasized the design aspect. However, having allowed the active participation of expert designers in the early phase of train design in the current project has led to a new era of domestic train development and the implementation of a way to meet demand flexibly with newly designed trains. The idea of a high-speed train in Korea and its design concept is well-conceived: a faster, more pleasant, and silent based Korean high-speed train that facilitates a new travel culture. A Korean-type of high-speed train is acknowledged by passengers who travel in such trains. The Korean high-speed prototype train has been born, combining aerodynamic air-cushioned design, which is the embodiment of Korean original design of forehead of power car minimized aerodynamic resistance using a curved car body profile, and the improvement of the interior design with ergonomics and the accommodation of the vestibule area through the study of passenger behavior and social culture that is based on the general passenger car.

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A Study on the Characteristics of a Reclaimed Marine Soils (Gupo Series) distributed on the Southern Coastal Area of Korea (우리나라 남해안(南海岸)에 분포(分布)된 간척지(干拓地) 토양(土壤)의 특성(特性)에 관(關)한 조사연구(調査硏究)(구포통(鳩浦統)에 관(關)하여))

  • Juug, Yeon-Tae;Um, Ki-Tae;Shin, Yong-Hwa
    • Korean Journal of Soil Science and Fertilizer
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    • v.9 no.2
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    • pp.99-105
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    • 1976
  • After investigation of soil characteristics the properties of a reclaimed marine soil (Gupo series) distributed along the southern coastal areas of Korea are summarized as follows: 1. Gupo soils distributed in the southern Ria coastal area are derived from rolling to hilly materials and are poorly sorted with less influences of river fluvial action. These soils have high content of sand compared with the broad fluvio-marine soils in the western coastal areas. 2. The morphological features of the poorly drained Gupo soils are greyish brown sandy loam with a few yellowish mottles in the surface horizon and are grey sandy loam with a few gravel in the sub-strata. The ground water table remains around 10-30cm below the surface. These soils, recently reclaimed younger deposits, do not show any evidence of illuviation. 3. The "n" value (about 0.8) of the Gupo soils indicates physically unripened soils. 4. pH value of these soils shows more than 8.0 throughout the profile. Organic matter contents are extremely low (around 0.5%) except 1.2 percent in the surface horizon. C.E.C. ranges from 7 to 9m.e/100g which is lower than average in the country. The ratios of extractable cations such as Ca, Mg, Na and K of the surface horizon are 20:7:4:1. Base saturation is more than 60%. Available phosphate content is very low that is less than 25 ppm. Electric conductivity of the soils at $25^{\circ}C$ ranges 7 to 12 mmhos/cm and increased with depth. 5. According to classification of soil based on physical ripening, the Gupo soils can be classified into "Unripe soils with half-ripe sub-soils". The soils could be classified into "Hydric Haplaquents" in the original of the 7th Approximation (1960), but into "Typic Haplaqents in the supplement of 7th approximation which the physical ripening condition is not clearly expressed. Soil Taxonomy, apparently the final version of the 7th approximation, defines the soils as "Haplic Hydraquents" that clearly show the condition of physical ripening as well as other properties. Other several classification systems applied do not describe physical ripening condition of the soils.

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Studies on the Absorption Capacity of Phosphorus of Korean Top-soils (우리나라 표층토(表層土)의 인산흡수력(燐酸吸收力)에 관(關)한 연구(硏究))

  • Park, C.S.;Han, K.H.;Lim, S.K.;Lee, J.H.
    • Korean Journal of Soil Science and Fertilizer
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    • v.2 no.1
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    • pp.1-13
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    • 1969
  • A total of 129 surface(upto 30 cm depth) soil samples were selected from the profile samples collected during reconnaissance soil survey in 1967, for the determination of phosphorus absorption co-efficient. The distribution range for each soil association has been established. The physicochemical factors affecting the phosphorus absorption coefficient have also been examined. The following general conclusions can be drown: 1. In general, the phosphorus absorption coefficient of the soil association of presently arable land are lower than the soils which are not in cultivation. 2. The higher the cation exchange capacity of soils, the higher is the phosphorus absorption coefficient. The factors governing phosphorus absorption coefficient in various soil associations are as follows: Parent Material Soil Association Governing Factor Fluvio marine Low Humic Gley Fluvio marine Alluvial Complex Narrow valley Siliceo mafic materials Red-yellow podzolic Redish Siliceo mafic materials Brown Lateritic Clay content Siliceous crystalline materials Lithosols C.E.C. & Clay content Alluvium Low Humic Alluvium Gley Alluvial Organic matter Siliceous crystalline materials Red-Yellow Podzolic Organic matter and clay content 4. The relation between phosphorus absorption coefficient determined by $(NH_4)_2HPO_4(y)$ and by the P 700 ppm $NaH_2PO_4(x)$ is $Y=2.716X+37(r=0.96^{**})$ which shows highly significant positive correlation and linear regression.

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