• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2019.05a
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• pp.278-280
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• 2019

Flow cytometer is one of the instrument that can measure various optical properties of a single cell or microparticle. These parameters including size, granularity, and fluorescence intensity are determined by the physical and optical interaction of the cells with excitation light source. However, users have some difficulties such as high cost, size of instrument, and limited fluorescence selectivity. In addition, abundant data is also unintentionally acquired even though user wants to have a single optical parameter. For these reasons, the use of flow cytometer is more challenging for researchers to apply their study. Therefore, the proposed study aims to develop a low-cost portable fluorescence acquisition system using a commercially available light-emitting diode and photodiode. It is designed by a 3D printer, and fluorescence selectivities are increased by changing of the light source / optical filter / detection sensor. Various number sets of fluorescently labeled cells were measured, and its feasibility was evaluated through the proposed system. As a result, acquried fluorescence intensities were proportional to the concentration of the cells and showed high linearity.

• Korean Journal of Plant Resources
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• v.29 no.4
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• pp.363-375
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• 2016

Potentilla kleiniana is a perennial herb beloning to Rosaceae family. Herein we investigated the effect of light intensity, light quality and chemical elicitor on plant growth and the accumulation of bioactive compounds in P. kleiniana. After 60 days of cultivation under different shading level [0% (200 μmol·m⁻²·s⁻¹), 35% (95 μmol·m⁻²·s⁻¹), 55% (65 μmol·m⁻²·s⁻¹), 75% (40 μmol·m⁻²·s⁻¹)] in the greenhouse, chlorophyll and carotenoid content were the highest under 35% treatment, however, plant height, leaf number and biomass were the highest under non-shading. As a result of cultivation among strong light condition as a control, florescence and three mixture light sources [red:white:blue (RWB) = 8:1:1, red:blue (RB) = 8:2, red:green:blue (RGB) = 8:1:1] as treatments in plant growth chamber (25 ± 2℃, 185 ± 3 μmol·m⁻²·s⁻¹), growth, biomass, chlorophyll content low difference between total phenolic compouds and flavonoid content were higher under RWB treatment. DPPH radical elimination ability was the highest under all treatments especially florescence and RGB treatment except control. As a result of treating chemical elicitor [salicylic acid (SA), methyl jasmonate (MeJA)] concentration (0, 50, 100, 200 μM) respectively, plant height, petiole diameter and biomass were higher under non-treatment, MeJA 50 μM. It was investigated that fresh weight and dry weight under MeJA 50 μM treatment were especially a little high. Total phenolic compounds and flavonoid content of SA 50 μM treatment was the highest but DPPH radical elimination ability was significantly the highest under MeJA 200 μM (88.65%) and MeJA 50 μM (87.84%) treatment. Thus, this study suggested that we determined optimal shading and light quality in the greenhouse and plant growth chamber also confirmed bioactive compound content, antioxidant ratio increase according to different chemical elicitation concentration.

• Journal of Forest and Environmental Science
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• v.17 no.1
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• pp.1-17
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• 2001

Forming a part of "Cooperative Practical Study for the Modernization of the Management of National Forest", this study was conducted to provide overall ecological information for the natural regeneration of major tree species on the basis of community structural attributes in the deciduous forest ecosystem. Followings are summarized characteristics of the natural regeneration for the selected tree species. Betula costata : Although large number of seeds are dispersed by wind, they require mineral soils to germinate. Thick litter layer could be an obstacle to germinate. After germination, the seedling requires large amount of light for successful establishment. Acer mono : Characterized by high shade tolerance and weak drought resistance, the seedling should be overcasted with more than 50% of canopy coverage. High stand density should be maintained to produce good quality of timber. The potential of coppice may be high. Ulmus laciniata : Since this species needs high rate of troll moisture and light, around 60% of canopy coverage should be maintained to retain moisture and incoming light. The competition with other vegetation should be removed for the favor of successful seedling establishment. Fraxinus mandshurica : This species requires moist mineral soils to germinate. After germination, the seedling needs large amount of light and moisture for successful establishment. Site preparation should be applied to reduce competition with weedy vegetation. Fraxinus rhynchophylla : Interval of large seed crops may be highly varied. Thick litter layer could be an obstacle to germinate. Site preparation should be applied to reduce competition with weedy vegetation so as to achieve successful seedling establishment. Quercus mongolica : Including the difficulty of seed supply by the consumption, thick litter layer and mountain bamboo cover could be the obstacle to germinate. More than 50% of relative light intensity is necessary to achieve successful seedling establishment. Kalopanax pictus : Thick litter layer could be an obstacle to germinate. The seedling needs large amount of light and moisture for successful establishment. Abies holophylla : In spite of high shade tolerance, the growth rate in sapling stage may be extremely slow. Cornus controversa : Seeds (drups) are consumed and dispersed by animals, tending to be not sufficient in seed supply. This species requires large amount of light for successful germination and seedling establishment. Tilia amurensis : The difficulty of seed supply might be expected with low seed purity and double dormancy. Since thick litter layer could be an obstacle to germinate, the species requires moist mineral soils for successful germination. The potential of coppice may be extremely high.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.4
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• pp.477-484
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• 2002

Ninety pre-puberal (6-7 months) female and 15 pre-puberal male Black Bengal goats were collected on the basis of their phenotypic characteristics from different parts of Bangladesh. Goats were reared under semi-intensive management, in permanent house. The animals were vaccinated against Peste Des Petits Ruminants (PPR), drenched with anthelmentics and deeped in 0.5% Melathion solution. They were allowed to graze 6-7 h along with supplemental concentrate and green forages. Concentrates were supplied either 200-300 g/d (low level feeding) or quantity that supply NRC (1981) recommended nutrient (high level of feeding). Different physiological, productive and reproductive characteristics of the breed were recorded. At noon (temperature=$95^{\circ}F$ and light intensity=60480 LUX) rectal temperature and respiration rate of adult male and female increased from 100.8 to $104.8^{\circ}F$ and 35 to 115 breath/min, indicated a heat stress situation. Young female attain puberty at an average age and weight of 7.2$\pm$0.18 months and 8.89$\pm$0.33 kg respectively. Mean age and weight at 1st kidding were 13.5$\pm$0.49 months and 15.3$\pm$0.44 kg respectively. It required 1.24-1.68 services per conception with an average gestation length of 146 days. At low level of feeding the postpartum estrus interval was 37$\pm$2.6 days, which reduced (p<0.05) with high feeding level to 21$\pm$6.9 days. Kidding interval also reduced (p<0.05) from 192 d at low feeding level to 177 d at high feeding level. On an average there were two kiddings/doe/year. Average litter sizes in the 1st, 2nd, 3rd and 4th parity were 1.29, 1.71, 1.87 and 2.17 respectively. Birth weights of male and female kids were 1.24 and 1.20 kg respectively, which increased (p<0.05) with better feeding. Although kid mortality was affected (p<0.05) by dam's weight at kidding, birth weight of kid, milk yield of dam, parity of kidding, season of birth, but pre-netal dam's nutrition found to be the most important factor. Kid mortality reduced from 35% at low level of feeding to 6.5% at high level of feeding of dam during gestation. Apparently, this was due to high (p<0.05) average daily milk yield (334 vs. 556 g/d) and heavier and stronger kid at birth at high feeding level.

• Korean Journal of Environment and Ecology
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• v.33 no.5
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• pp.605-619
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• 2019

The objective of this study was to analyze eutrophication characteristics, empirical model analysis, and variation of water quality according to monsoon intensity in Hapcheon Reservoir for 16 years from 2002 to 2017. Long-term annual water quality analysis showed that Hapcheon Reservoir was in a meso-nutrition to eutrophic condition, and the eutrophic state intensified after the summer monsoon. Annual rainfall volume (high vs. low rainfall) and the seasonal intensity in each year were the key factors that regulate the long-term water quality variation provided that there is no significant change of the point- and non-point source in the watershed. Dry years and wet years showed significant differences in the concentrations of TP, TN, BOD, and conductivity, indicating that precipitation had the most direct influence on nutrients and organic matter dynamics. Nutrient indicators (TP, TN), organic pollution indicators (BOD, COD), total suspended solids, and chlorophyll-a (Chl-a), which was an estimator of primary productivity, had significant positive relations (p<0.05) with precipitation. The Chl-a concentration, which is an indicator of green algae, was highly correlated with TP, TN, and BOD, which differed from other lakes that showed the lower Chl-a concentration when nutrients increased excessively. Empirical model analysis of log-transformed TN, TP, and Chl-a indicated that the Chl-a concentration was linearly regulated by phosphorus concentration, but not by nitrogen concentration. Spatial regression analysis of the riverine, transition, and lacustrine zones of $log_{10}TN$, $log_{10}TP$, and $log_{10}CHL$ showed that TN and Chl-a had significant relations (p<0.005) while TN and Chl-a had p > 0.05, indicating that phosphorus had a key role in the algal growth. Moreover, the higher correlation of both $log_{10}TP$ and $log_{10}TN$ to $log_{10}CHL$ in the riverine zone than the lacustrine zone indicated that there was little impact of inorganic suspended solids on the light limitation in the riverine zone.

• Proceedings of the Korean Vacuum Society Conference
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• 2016.02a
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• pp.280.1-280.1
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• 2016

Generally InGaN/GaN green light emitting diode (LED) exhibits the low quantum efficiency (QE) due to the large lattice mismatch between InGaN and GaN. The QE of InGaN-based multiple quantum wells (MQWs) is drastically decreased when an emission wavelength shifts from blue to green wavelength, so called "green gap". The "green gap" has been explained by quantum confined Stark effect (QCSE) caused by a large lattice mismatch. In order to improve the QE of green LED, undoped graded short-period InGaN/GaN superlattice (GSL) and Si-doped GSL (SiGSL) structures below the 5-period InGaN/GaN MQWs were grown on the patterned sapphire substrates. The luminescence properties of InGaN/GaN green LEDs have been investigated by using photoluminescence (PL) and time-resolved PL (TRPL) measurements. The PL intensity of SiGSL sample measured at 10 K shows stronger about 1.3 times compared to that of undoped GSL sample, and the PL peak wavelength at 10 K appears at 532 and 525 nm for SiGSL and undoped GSL, respectively. Furthermore, the PL decay of SiGSL measured at 10 K becomes faster than that of undoped GSL. The faster decay for SiGSL is attributed to the increased wavefunction overlap between electron and hole due to the screening of piezoelectric field by doped carriers. These PL and TRPL results indicate that the QE of InGaN/GaN green LED with GSL structure can be improved by Si-doping.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.34 no.1
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• pp.73-76
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• 2010

The issue of inspection and characterization of microstructures has emerged as a major consideration in design, fabrication, and detection of MEMS devices. However, the conventional measurement techniques, including scanning electron microscopy (SEM) imaging, atomic force microscopy (AFM) scanning, and mechanical surface profiler, require often destructive process or may be difficult to measure with a wafer scale. In this paper, we characterize the surface profiles of microstructures using an optical scanner based on a DVD pick-up module. Scanning images of the microstructures are successfully generated using the intensity of reflected light from different depths of the surface profiles, based on the focus error signal (FES) from photodiodes. It is shown that the proposed optical scanner can be used as an alternative measurement system with high performance and low cost, compared to conventional measurement techniques.

• Fashion & Textile Research Journal
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• v.20 no.1
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• pp.63-74
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• 2018

The purpose of this study is to investigate the differences in riding characteristics and helmet wearing conditions between bicycle and PMV riders so that the basis data necessary for the development of suitable helmets for each group is provided. For this purpose, riding characteristics and helmet wearing conditions of bicycle and PMV users were investigated using online survey method and then the survey results were interpreted by in-depth interview conducted for bicycle and PMV users. The online survey results showed that the PMV group showed shorter driving distance and more driving frequency than bicycle group. This short driving distance was due to the limitation of battery capacity of PMVs. Helmet wearing rate was significantly lower in PMV group than in bicycle group, which was associated with relatively low chances to drive long distance on the motorway. In the PMV group, the 'urban helmets' were mainly used, in which the appearance of helmet was priorized, but in the bicycle group, the 'road cycle helmets' were mainly used, in which the light weight or ventilation were priorized. Urban helmets caused stronger pain and more fitting problems than road cycle helmets because the head shapes of Koreans were not properly applied to the helmet design. Since the fitting problem and pain intensity were the important causes that making PMV users not wear the helmets, it is necessary to develop the urban helmets reflecting the head shapes of Koreans in order to increase the helmet wearing rate of the PMV users.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.3
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• pp.501-505
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• 2020

Objective: The purpose of this study was to investigate the effects of aging methods (AM) i.e. dry-aging (DA) and wet-aging (WA) on the physicochemical properties and in vitro digestibility of proteins in beef short loin. Methods: Short loins (M. longissmus lumborum), were trimmed and boned-out on the fifth day postmortem, from a total of 18 Hanwoo, which were purchased from a commercial slaughterhouse. Short loins were separated randomly grouped into one of the three treatments: control, WA (1℃, 7 days), and DA (1℃, 0.5 m/s, 85% relative humidity [RH], 30 days). Results: Dry-aged beef (DAB) exhibited higher pH, water holding capacity (WHC), myofibrillar fragmentation index (MFI), and digestibility, however lower lightness, redness, and yellowness values, cooking loss, and shear force (SF), than those of wet-aged beef (WAB) (p<0.05). The myosin light chain band intensity of DAB was higher than that of control and WAB in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The in vitro digestibility of aged beef was highly (p<0.001) correlated to physicochemical properties except WHC. The correlation coefficient between AMs and WHC was higher than that between AM and SF (p<0.05) or MFI (p<0.001). A high correlation was observed between SF and MFI (p<0.001). Conclusion: Thus, we believe that DAB is more advantageous than WAB owing to its high digestibility and WHC and low SF.

• Ocean and Polar Research
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• v.36 no.4
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• pp.437-445
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• 2014

A time-series sediment trap was operated at a water depth of 4950 m from July 2003 to May 2004 at KOMO station ($10^{\circ}30^{\prime}N$, $131^{\circ}20^{\prime}W$) in the northeastern equatorial Pacific, with the aim of understanding the temporal variation of planktonic foraminifera assemblages in response to the seasonal shift of Inter-Tropical Convergence Zone (ITCZ). A total of 22130 planktonic foraminifera specimens belonging to 30 species and 11 genera were identified, which shows a distinct seasonal variation with high values (125~288 specimens $m^{-2}day^{-1}$) in the winter to spring (December-May) and low values (16~23 specimens $m^{-2}day^{-1}$) in the fall (September-November). In addition, seasonal ecological differences of foraminifera assemblages are distinctly recognizable: omnivorous foraminifera occurred predominantly during the summer season, whereas herbivorous ones were dominant during the winter season. Such seasonal variations correspond to the seasonal shift of the ITCZ. Enhanced occurrence of herbivorous species during the winter-spring season seems a result of surface water mixing generated by the southward shift of the ITCZ. The increase in omnivorous species during the summer season may be due to the northward movement of the ITCZ caused by weakened wind speed, resulting in the intensification of water column stratification and nutrient-poor environment. A significant reduction of planktonic foraminifera specimens during the fall is attributed to heavy precipitation and reduction in light intensity.