• Title/Summary/Keyword: low light intensity

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Study on the Enzyme Activity in Leaf-Burning Disease of Panax ginseng C.A. Meyer (인삼엽요병에서 효소활성도의 변화)

  • 양덕조;김명원
    • Journal of Ginseng Research
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    • v.13 no.1
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    • pp.92-97
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    • 1989
  • This study investigated the effects of high light intensity (100 KLw) and high temperature (45 ℃, dark) on enzyme (glucose-6-phosphate dehydrogenase, acid phosphatase, catalase, peroxidase, and proteinase) activities and characteristics of Panax ginseng C.A. Meyer leaves. Enzyme activity and protein content decreased rapidly under treatment with high light intensity In P ginseng the thermal stabilities of catalase and peroxidase were high (above 70%), and the coagulation rates of soluble proteins were low (below 17%). Therefore, the decrease in enzyme activity and protein content was not caused by increase in leaf temperature due to the high light intensity, but by increase in proteolytic activities. The photochemical formation rate of superoxide radical (O-2) was higher in the P ginseng leaf extracts than in Solanum nigmm, and was accelerated by addition of crude saponin to the buffer extracts.

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Continuous Production of Natural Colorant, Betacyanin, by Beta vulgaris L. Hairy Root

  • Kim, Sun-Hee;Ahn, Sang-Wook;Bai, Dong-Kyu;Kim, Kwang-Soo;Hwang, Baik;Lee, Hyeon-Yong
    • Journal of Microbiology and Biotechnology
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    • v.9 no.6
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    • pp.716-721
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    • 1999
  • It has been known that continuous cultivation of hairy root is difficult to maintain for a long period of time compared to the microbial and callus cultures. Chemostat cultivation was successfully carried out in order to economically produce a plant-based colorant, betacyanin, from red beet hairy root for more than 85 days in a 14-1 fermentor. The result from the chemostat cultivation was compared to those of the batch and fed-batch cultivations of red beet hairy roots. It was shown that hairy root reached its steady state within 50 days of the cultivation, and then maintained for about 25-30 days in a wide range of dilution rates. Total betacyanin production from the continuous process was also calculated to be 2.65g at 0.28(l/d) of dilution rate, compared to 0.196g from fed-batch cultivation. It was found that betacyanin production was a partially growth related process, yielding 0.376 mg/g-fresh wt. cell and $1.89{\times}10^{-5}$ mg/g-fresh wt. cell/d, with 0.92 of correlation factor in a partial growth-product model. It was also shown that the cell growth required was relatively large for maintenance amount of energy at a low dilution rate. The growth of hairy root was inhibited by high light intensity in following a photo-inhibition model. The growth parameters were estimated to be 0.3(l/d), $10.56kcal/\textrm{m}^2/h$,{\;}and{\;}35.81kcal/\textrm{m}^2/h$ for the maximum specific growth rate, half saturation light intensity, and inhibition light intensity, respectively.

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LED Fiber-Optic Lighting Devices Developed for Medical Assistance for the Local Treatment Retractor (국부치료를 위한 Retractor용 LED 광섬유 의료보조조명 장치 개발)

  • Eo, Ik-soo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.2
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    • pp.666-671
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    • 2016
  • This paper introduces a device providing close local lighting to the affected part, where the operation-purpose astral lamp alone cannot shed light directly, in an operation room of hospitals or clinics, which helps clinical doctors perform safe treatment of the affected part deep inside a human body. This medical assisting lighting is a device necessary to prevent fatal operation failures, which can occur with minute operation processes, such as tumor removal or angiorrhaphy. The components, such as the light source and power supply were designed to be low power consuming and small in size, have a narrow angle lens was used to increase the light spreading effect and focused illumination. The end-caps of the light transmission device using an optical cable and the lighting device were designed in the waterproof type to enable disinfection of these devices after use for the next patients. According to the measurement of the light source properties made after development of the retractor lighting device, the illumination intensity was 490 lux, the brightness was $11,550cd/m^2$, general color rendering index was 78, color temperature was 11,000K, and the intensity distribution was even, which were confirmed to be adequate for medical assisting local lighting.

Effects of Light Intensity and Electrical Conductivity Level on Photosynthesis, Growth and Functional Material Contents of Lactuca indica L. 'Sunhyang' in Hydroponics (수경재배에서 광도와 양액 농도가 베이비 산채 왕고들빼기 '선향' 광합성과 생육 및 기능성 물질 함량에 미치는 영향)

  • Kim, Jae Kyung;Jang, Dong Cheol;Kang, Ho Min;Nam, Ki Jung;Lee, Mun Haeng;Na, Jong Kuk;Choi, Ki Young
    • Journal of Bio-Environment Control
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    • v.30 no.1
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    • pp.1-9
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    • 2021
  • This study was conducted to examine the changes of photosynthesis, growth, chlorophyll contents and functional material contents in light intensity and EC concentration of wild baby leaf vegetable, Indian lettuce (Lactuca indica L. cv. 'Sunhyang') in DFT hydroponics. The cultivation environment was 25±1℃ of temperature and 60±5% of relative humidity in growth system. At 14 days after sowing, combination effect of light intensity (Photosynthetic Photon Flux Density (PPFD 100, 250, 500 µmol·m-2·s-1) and EC level (EC 0.8, 1.4, 2.0 dS·m-1) of nutrient solution was determined at the baby leaf stage. The photosynthesis rate, stomatal conductance, transpiration rate and water use efficiency of Indian lettuce increased as the light intensity increased. The photosynthesis rate and water use efficiency were highest in PPFD 500-EC 1.4 and PPFD 500-EC 2.0 treatment. The chlorophyll content decreased as the light intensity increased, but chlorophyll a/b ratio increased. Leaf water content and specific leaf area decreased as light intensity increased and a negative correlation (p < 0.001) was recognized. Plant height was the longest in PPFD 100-EC 0.8 and leaf number, fresh weight and dry weight were the highest in PPFD 500-EC 2.0. Anthocyanin and total phenolic compounds were the highest in PPFD 500-EC 1.4 and 2.0 treatment, and antioxidant scavenging ability (DPPH) was high in PPFD 250 and 500 treatments. Considering the growth and functional material contents, the proper light intensity and EC level for hydroponic cultivation of Indian lettuce is PPFD 500-EC 2.0, and PPFD 100 and 250, which are low light conditions, EC 0.8 is suitable for growth.

Cloning of CAB cDNA encoding chlorophyll a/b binding protein of photosystem II in Korean ginseng and Use in Plant (고려인삼 광계 II Chlorophyll a/b binding Protein 유전자(CAB)의 cloning 및 식물에의 활용연구)

  • 김갑식;이기원;이종철;여운형;채순용;박은경
    • Journal of the Korean Society of Tobacco Science
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    • v.21 no.2
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    • pp.152-159
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    • 1999
  • A CAB cDNA clone(pKGCAB) encoding the light harvesting chlorophyll a/b binding protein of the semi-shade plant, Korean ginseng(Panax ginseng C. A. Meyer) was isolated by the one-way path random sequencing of ginseng cDNA library clones and transgenic tobacco plants(Nicotiana tabacum NC82) were produced by the transformation of this ginseng CAB gene in use of Agrobacterium tumefaciens LBA4404. The CAB gene showed type 1 structure of LHCP-II, 84% similarity in nucleotide sequence and 92% in amino acid sequence to that of Nicotiana tabacum CAB40, respectively. Seed germination and initial growth of the transgenic tobacco plants transformed with the cDNA fragment were accelerated under low light intensity compared with those of normal tobacco plant, that may result from the higher light sensitivity of the transgenic plants than that of the normal.

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The Design of Flash Lantern using LED for aironautical ground lights (LED를 이용한 항공등의 점멸 방법에 대한 연구)

  • Jeong, Hak-Geun;Jung, Bong-Man;Park, Suk-In;Yu, Seung-Won;Shin, Kyu-Yong
    • Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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    • 2006.05a
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    • pp.157-159
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    • 2006
  • The advantages of LED(Light Emitting Diode) are low electric power consumption, long life time and excellent visibility. And a LED light source does not include the mercury(Hg) and or a filament, it is expected as an environmentally friendly next generation light source with its good reliability. In order to design and develope the flash lantern using LED for aironautical ground lights, technical trends and new standards about the aironautical ground lights were inspected, and power consumption and flash time for effective luminous intensity of aironautical ground lights were analyzed.

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Basic Examination on 3D Measuring System Using Pulse-Compression

  • Fujimoto Ikumatsu;Ando Shigeru
    • International Journal of Precision Engineering and Manufacturing
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    • v.6 no.4
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    • pp.60-66
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    • 2005
  • In this paper, we propose the basic measurement method of a 3D digitizer using a CCD camera in detail. In the localization measurement with a CCD camera, the effect of the background light and the sensitivity consideration are always problems in realizing a high precision. In this research, a new measurement principle is proposed in which the pulse compression technique known in radar is used to eliminate the effect of background light even under a low intensity light source, and the coordinate values on the CCD camera image plane are determined accurately. From the quantitative evaluation of the S/N ratio improvement and the fundamental experiment, it is verified that a substantial improvement in the S/N ratio is realized for both the background noise and the pixel noise and that a resolution of less than the pixel is sufficiently possible.

EFFECT OF LIGHT IRRADIATION MODES ON THE MARGINAL LEAKAGE OF COMPOSITE RESIN RESTORATION (광조사 방식이 복합레진 수복물의 변연누출에 미치는 영향)

  • 박은숙;김기옥;김성교
    • Restorative Dentistry and Endodontics
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    • v.26 no.4
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    • pp.263-272
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    • 2001
  • The aim of this study was to investigate the influence of four different light curing modes on the marginal leakage of Class V composite resin restoration. Eighty extracted human premolars were used. Wedge-shaped class Y cavities were prepared on the buccal surface of the tooth with high-speed diamond bur without bevel. The cavities were positioned half of the cavity above and half beyond the cemento-enamel junction. The depth, height, and width of the cavity were 2 mm, 3 mm and 2 mm respectively. The specimens were divided into 4 groups of 20 teeth each. All the specimen cavities were treated with Prime & Bond$^{R}$ NT dental adhesive system (Dentsply DeTrey GmbH, Germany) according to the manufacturer's instructions and cured for 10 seconds except group VI which were cured for 3 seconds. All the cavities were restored with resin composite Spectrum$^{TM}$ TPH A2 (Dentsply DeTrey GmbH, Germany) in a bulk. Resin composites were light-cured under 4 different modes. A regular intensity group (600 mW/${cm}^2$, group I) was irradiated for 30 s, a low intensity group (300 mW/${cm}^2$, group II) for 60 s and a ultra-high intensity group (1930 mW/${cm}^2$, group IV) for 3 s. A pulse-delay group (group III) was irradiated with 400 mW/${cm}^2$ for 2 s followed by 800 mW/${cm}^2$ for 10 s after 5 minutes delay. The Spectrum$^{TM}$ 800 (Dentsply DeTrey GmbH, Germany) light-curing units were used for groups I, II and III and Apollo 95E (DMD, U.S.A.) was used for group IV. The composite resin specimens were finished and polished immediately after light curing except group III which were finished and polished during delaying time. Specimens were stored in a physiologic saline solution at 37$^{\circ}C$ for 24 hours. After thermocycling (500$\times$, 5-55$^{\circ}C$), all teeth were covered with nail varnish up to 0.5 mm from the margins of the restorations, immersed in 37$^{\circ}C$, 2% methylene blue solution for 24 hours, and rinsed with tap water for 24 hours. After embedding in clear resin, the specimens were sectioned with a water-cooled diamond saw (Isomet$^{TM}$, Buehler Co., Lake Bluff, IL, U.S.A.) along the longitudinal axis of the tooth so as to pass the center of the restorations. The cut surfaces were examined under a stereomicroscope (SZ-PT Olympus, Japan) at ${\times}$25 magnification, and the images were captured with a CCD camera (GP-KR222, Panasonic, Japan) and stored in a computer with Studio Grabber program. Dye penetration depth at the restoration/dentin and the restoration/enamel interfaces was measured as a rate of the entire depth of the restoration using a software (Scion image, Scion Corp., U.S.A.) The data were analysed statistically using One-way ANOVA and Tukey's method. The results were as follows : 1. Pulse-Delay group did not show any significant difference in dye penetration rate from other groups at enamel and dentin margins (p>0.05) 2. At dentin margin, ultra-high intensity group showed significantly higher dye penetration rate than both regular intensity group and low intensity group (p<0.05). 3. At enamel margin, there were no statistically significant difference among four groups (p>0.05). 4. Dentin margin showed significantly higher dye penetration rate than enamel margin in all groups (p<0.05).

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A Novel Ultraviolet Sensor using Photoluminescent Porous Silicon (광 루미네슨스 다공질 실리콘을 이용한 새로운 자외선 센서)

  • Min, Nam-Gi;Go, Ju-Yeol;Gang, Cheol-Gu
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.50 no.9
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    • pp.444-449
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    • 2001
  • In this paper, a novel ultraviolet sensor is presented based on a photoluminescent porous silicon. Porous silicon layer was formed by chemical etching of surface of pn junction in a $HF(48%)-HNO_3(60%)-H_20$ solution. Incident ultraviolet(UV) light is converted to visible light by photoluminescent porous silicon layer, and then this visible light generates electron-hole pairs in the pn junction, which produces a photocurrent flow through the device. In order to maximize detection efficiency, the peak sensitivity wavelength of the pn junction diode was matched with the peak wavelength of Photoluminescence from porous silicon layer. The porous silicon ultraviolet sensor showed a large output current as UV intensity increases and but very low sensitivity to visible light. The detection sensitivity of porous silicon sensor was calculated as 2.91mA/mW. These results are expected to open up a possibility that the present porous silicon sensor can be used for detecting UV light in a visible background, compared to silicon UV detectors which have an undesirable response to visible light.

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Development of Projection Scanbeam-SLA using Liquid Crystal Display and Visible Light Emitting Diode (LCD와 가시광선 LED를 사용한 전사방식의 Scanbeam-SLA 개발)

  • Yoon, Su Hyun;Park, In Baek;Kim, Min Sub;Jo, Kwang Ho;Lee, Seok Hee
    • Journal of the Korean Society for Precision Engineering
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    • v.30 no.3
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    • pp.340-348
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    • 2013
  • In Projection Stereolithography Apparatus (PSLA), Digital Micromirror Device (DMD) and Liquid Crystal Display (LCD) are used as a beam pattern generator. The DMD shows high resolution, but it is mostly applied in micro stereolithography due to high cost and fabricable area. In LCD, the size of pattern beam is freely controlled due to various panel sizes. The LCD, however, has some limitations such as short life time by the high power light source, non-uniform light intensity of pattern beam and low transmittance of UV-light. To solve these problems in LCD-based PSLA, a Scanbeam-SLA with LCD of 19 inches and visible LED-array is developed. In this system, the light module works like a scanner for uniform illumination. The system configuration, working principle and fabrication examples are addressed in this study.