• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
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• pp.277.2-277.2
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• 2013

Petri dishes and glass slides have been widely used as general substrates for in vitro mammalian cell cultures due to their culture viability, optical transparency, experimental convenience, and relatively low cost. Despite the aforementioned benefit, however, the flat two-dimensional substrates exhibit limited capability in terms of realistically mimicking cellular polarization, intercellular interaction, and differentiation in the non-physiological culture environment. Here, we report a protocol of culturing embryonic rat hippocampal neurons on the electro-spun polymeric network and the results from examination of neuronal cell behavior and network formation on this culture platform. A combinatorial method of laser-scanning confocal fluorescence microscopy and live-cell imaging technique was employed to track axonal outgrowth and synaptic connectivity of the neuronal cells deposited on this model culture environment. The present microfiber-based scaffold supports the prolonged viability of three-dimensionally-formed neuronal networks and their microscopic geometric parameters (i.e., microfiber diameter) strongly influence the axonal outgrowth and synaptic connection pattern. These results implies that electro-spun fiber scaffolds with fine control over surface chemistry and nano/microscopic geometry may be used as an economic and general platform for three-dimensional mammalian culture systems, particularly, neuronal lineage and other network forming cell lines.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2011년도 추계학술발표대회
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• pp.19.1-19.1
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• 2011

We report on the metal-insulator transition that occurs as a function of film thickness in ultrathin $La_{0.75}Sr_{0.25}VO_3$ films. The metal-insulator transition displays a critical thickness of 5 unit cell. Above the critical thickness, metallic films exhibit a temperature driven metal-insulator transition with weak localization behavior. With decreasing film thickness, oxygen octahedron rotation in the films increases, causing enhanced electron-electron correlation. The electron-electron correlations in ultrathin films induce the transition from metal to insulator in addition to Anderson localization.

• Nuclear Engineering and Technology
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• 제55권11호
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• pp.4274-4281
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• 2023

Blanket is of vital importance for engineering application of the fusion reactor. Nuclear heat deposition in materials is the main heat source in blanket structure. In this paper, the three-dimensional method for thermal-hydraulics/neutronics coupling analysis is developed and applied for the full-scale module of the helium-cooled ceramic breeder tritium breeding blanket (HCCB TBB) designed for China Fusion Engineering Test Reactor (CFETR). The explicit coupling scheme is used to support data transfer for coupling analysis based on cell-to-cell mapping method. The coupling algorithm is realized by the user-defined function compiled in Fluent. The three-dimensional model is established, and then the coupling analysis is performed using the paralleled Coupling Analysis of Thermal-hydraulics and Neutronics Interface Code (CATNIC). The results reveal the relatively small influence of the coupling analysis compared to the traditional method using the radial fitting function of internal heat source. However, the coupling analysis method is quite important considering the nonuniform distribution of the neutron wall loading (NWL) along the poloidal direction. Finally, the structure optimization of the blanket is carried out using the coupling method to satisfy the thermal requirement of all materials. The nonlinear effect between thermal-hydraulics and neutronics is found during the blanket structure optimization, and the tritium production performance is slightly reduced after optimization. Such an adverse effect should be thoroughly evaluated in the future work.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2012년도 제42회 동계 정기 학술대회 초록집
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• pp.549-549
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• 2012

Early detection of cancer biomarkers in the blood is of vital importance for reducing the mortality and morbidity in a number of cancers. From this point of view, immunosensors based on nanowire (NW) and carbon nanotube (CNT) field-effect transistors (FETs) that allow the ultra-sensitive, highly specific, and label-free electrical detection of biomarkers received much attention. Nevertheless 1D nano-FET biosensors showed high performance, several challenges remain to be resolved for the uncomplicated, reproducible, low-cost and high-throughput nanofabrication. Recently, two-dimensional (2D) graphene and reduced GO (RGO) nanosheets or films find widespread applications such as clean energy storage and conversion devices, optical detector, field-effect transistors, electromechanical resonators, and chemical & biological sensors. In particular, the graphene- and RGO-FETs devices are very promising for sensing applications because of advantages including large detection area, low noise level in solution, ease of fabrication, and the high sensitivity to ions and biomolecules comparable to 1D nano-FETs. Even though a limited number of biosensor applications including chemical vapor deposition (CVD) grown graphene film for DNA detection, single-layer graphene for protein detection and single-layer graphene or solution-processed RGO film for cell monitoring have been reported, development of facile fabrication methods and full understanding of sensing mechanism are still lacking. Furthermore, there have been no reports on demonstration of ultrasensitive electrical detection of a cancer biomarker using the graphene- or RGO-FET. Here we describe scalable and facile fabrication of reduced graphene oxide FET (RGO-FET) with the capability of label-free, ultrasensitive electrical detection of a cancer biomarker, prostate specific antigen/${\alpha}$ 1-antichymotrypsin (PSA-ACT) complex, in which the ultrathin RGO channel was formed by a uniform self-assembly of two-dimensional RGO nanosheets, and also we will discuss about the immunosensing mechanism.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2002년도 춘계학술대회 논문집
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• pp.616-619
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• 2002

Spin casting is one of useful methods to manufacture metal parts with low mold cost and short delivery time. But the silicon rubber based conventional method has several problems such as poor dimensional accuracy, limitation in casting materials and its dependency on speciality in meld making process. To solve those problems, this paper suggests a steel based mold making method using direct CNC machining and the experimental results shows that the parts from the developed method has better dimensional accuracy and surface roughness than those from the conventional method.

• International Journal of Aeronautical and Space Sciences
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• 제1권1호
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• pp.36-47
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• 2000

Three-dimensional compressible turbulent flow fields within the passage of a diffusing S-duct have been simulated by solving the Navier-Stokes equations with SIMPLE scheme. The average inlet Mach number is 0.6 and the Reynolds number based on the inlet diameter is $1.76{\times}10^6$ The extended $k-{\varepsilon}$ turbulence model is applied to modeling the Reynolds stresses. Computed results of the flow in a circular diffusing S-duct provide an understanding of the flow structure within a typical engine inlet system. These are compared with experimental wall static-pressure, total-pressure fields, and secondary velocity profiles. Additionally, boundary layer thickness, skin friction values, and streamlines in the symmetric plane are presented. The computed results depict the interaction between the low energy flow by the flow separation and the high energy flow by the reversed duct curvature. The computed results obtained using the extended $k-{\varepsilon}$ turbulence model.

• 대한치과보철학회지
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• 제37권3호
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• pp.383-394
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• 1999

The indirect technique for making cast restoration requires that dies be as accurate and durable as possible. Currently, stone is the most commonly used material for die. However, it has some problems such as the weakness in its strength and low abrasion resistance. Recently, epoxy resin die systems have become available. The purpose of this study was to examine two commercially available resin die systems and evaluate some characteristics for their clinical performance. This study evaluated the dimensional accuracy of epoxy resins and their wettability with impression materials. In this study, the first experiment was about dimensional accuracy of different die materials. The master model was made of stainless steel. 10 models were made of two epoxy resins (Die-epoxy, Tri-epoxy) and a die stone (Fujirock) each. Occlusal diameter (Dimension I), occluso-gingival height (Dimension II), and interabutment distance (Dimension III) were measured in each model. Next, the contact angles of die materials with impression materials were observed. The blocks were made of polyether, hydrophilic additional silicone, polysulfide impression materials. By drop-ping the same amount (0.05ml) of Tri-epoxy, Die-epoxy, and die stone on the blocks, 10 samples of each die material were made. After setting of materials, the contact angles were measured. The results of this study were as follows. 1. The expansion of stone die and the shrinkage of resin dies in occlusal diameter were observed, and stone and Tri-epoxy were expanded and Die-epoxy was shrinked in occluso-gingival height. There was little change among materials in interabutment distance (p<0.05). 2. In comparison with the master model Tri-epoxy had the least variation in measurement of the three die systems examined. Die-epoxy was next, and die stone showed the greatest variation. 3. The compatibility of die stone for polyether, hydrophilic additional silicone, polysulfide decreased in order, wherease epoxy materials had the decreased compatibility for polyether and polysulnde, hydrophilic additional silicone in order. It was not statistically different between polyether and polysulfide (p<0.05). 4. The contact angles of Tri-epoxy, Die-epoxy, die stone were getting bigger in order.

• Journal of the Korean Wood Science and Technology
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• 제42권6호
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• pp.691-699
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• 2014

용융점이 $138^{\circ}C$인 Bi-Sn 혼합합금 주입방법을 이용한 용융합금주입목재복합체의 제조를 통해 목재의 단점인 치수 안정성과 내구성의 개선을 하고자 3가지 주요 침 활엽수 수종의 수종별 최적주입조건을 구명하고, 주입에 따른 금속주입목재복합체의 중량증가율, 열전도도, 전기저항 등의 물리적 성질과 기계적 성질을 실험하여 다음과 같은 결과를 얻었다. 1. 금속주입목재의 최적처리 조건은 $185^{\circ}C$, 진공시간 10분 조건하에서 radiata pine의 경우 $10kgf/cm^2$ 가압시간 2분 30초, red oak는 $30kgf/cm^2$, 가압시간 10분, white oak는 $50kgf/cm^2$, 가압시간 10분에서 최적의 주입조건을 나타냈다. 2. 금속주입목재복합체의 외관적 특징은 전체적으로 재색은 회백색을 나타냈으며, 목재의 무늬를 그대로 유지하고 있다. 3. 침엽수 수종인 radiata pine은 저온용융합금주입처리로 인해 중량증가율은 12배, 밀도는 $6.13g/cm^3$으로 15배나 증가하였으나, 인테리어용으로 주로 사용되는 red oak와 white oak 등의 활엽수의 경우 목재해부학적 특성으로 의해 radiata pine보다는 낮은 5~6배의 밀도증가율을 나타냈다. 4. 주입된 목재는 침 활엽수 모두 금속주입으로 인해 수분흡수율과 두께팽윤율이 현저히 감소하였고, 매우 높은 치수 안정성을 나타냈으며, 금속주입목재복합체의 휨강도, 휨영계수, 경도 및 전기전도도와 열전도도 등도 무처리목재보다 매우 크게 향상되었다.

• 폴리머
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• 제39권3호
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• pp.394-402
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• 2015

폴리아크릴로니트릴(PAN) 필름에 히드록실아민(HA)을 반응시켜 히드록시기와 아민기를 생성시키고, 히드라진으로 처리하여 가교결합을 도입시키는 방법을 검토하였다. HA와 히드라진을 각각 처리하거나 2단계 또는 동시 처리하여 개질시킨 PAN 시료들의 반응도, 물 및 N,N'-디메틸포름아미드(DMF) 팽윤도, FTIR 스펙트럼, 원소함량, 열분석 결과들을 분석하여 효율적으로 기능기와 가교결합을 도입시키는 방법을 제시하였다. PAN 필름은 HA와의 반응에 의해 기능기가 도입되고 친수성이 향상되지만 형태안정성이 감소하며, 히드라진 처리에 의해 DMF 팽윤도가 감소한다. 히드라진 및 HA를 2단계로 처리하면 형태안정성은 향상되지만 기능기 도입이 어려워지고, 히드라진과 HA를 동시에 처리하면 형태안정성과 친수성을 효율적으로 향상시킬 수 있다.

• 자원환경지질
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• 제28권5호
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• pp.469-480
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• 1995

Gravity and magnetic surveys were carried out to investigate the three-dimensional configuration and characteristics of the landfills at Nanjido waste disposal site. For terrain correction and three-dimensional density inversion of gravity data an algorithm, which calculates the gravity effect of a three-dimensional body by using the solid angle method, is developed. This algorithm has been proved to give more accurate terrain correction values for the small survey area having varied topography like Nanjido site as compared with widely used methods such as Hammer's method and multiquadric equation method. Density inversion of gravity anomaly data gives very useful information about the lateral and vertical variation of the landfills, which can be used to discriminate the kinds of wastes. The average density of filled materials appears to be $1.7\;g/cm^3$ which is much higher than the value $(0.8\;g/cm^3)$ estimated by Seoul City. The lateral variation of density shows high correlation with the pattern of ongoing depression of the landfills. The northern region of the landfill no. 1, which shows low density and high depression, is closely associated with the industrial waste and sludge filled area. The magnetic anomaly data provide information about relative concentration of magnetic materials, which is also very useful to investigate characteristics of the fills. Several high positive anomaly regions on the reduced-to-pole magnetic anomaly map are appeared to be associated with the industrial waste fills, but certain industrial waste fills show low negative anomalies. This kind of magnetic information can be used in selecting drilling locations over landfills away from buried metal products during the stabilization process.