• Title/Summary/Keyword: long-term preservation test

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Effect of Fermented Garlic Extract Containing Nitric Oxide Metabolites on Impairments of Memory and of Neural Plasticity in Rat Model of Vascular Dementia (산화질소 대사체 함유 마늘 발효 추출물 이용 혈관성 치매 흰쥐 모델의 기억력 및 신경가소성 장애 개선 효과)

  • Zhang, Xiaorong;Moon, Se Jin;Kim, Yoo Ji;Jeong, Sun Oh;Kim, Min Sun
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.36 no.2
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    • pp.59-65
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    • 2022
  • Rodent model for chronic cerebral hypoperfusion caused by bilateral carotid artery occlusion (BCAO) show clinically relevant evidences for vascular dementia and impairments of synaptic plasticity in the hippocampus. The purpose of this study was to evaluate effect of fermented garlic (F-Garlic) extract with NO metabolites on cognitive behaviors, synaptic plasticity, and molecular events in the hippocampus following BCAO. Adult male Sprague-Dawley rats were randomly divided three experimental groups into: control+water; BCAO+water; BCAO+F-Garlic. Animals were treated with oral administration of F-Garlic in tap water as a drinking water after surgery for 4 weeks. On passive avoidance test and Y-maze test, BCAO+water showed a significant decrease in step-through latency and spontaneous alteration, indicating deficit of hippocampal memory formation but the treatment of F-Garlic significantly increased these cognitive behaviors. In control+water, a robust increase in the amplitude of evoked field excitatory postsynaptic potentials was observed by theta burst stimulation to hippocampal neural circuit indicating formation of long-term potentiation (LTP) in the hippocampal CA1. BCAO+water showed a highly significant deficit in LTP induction 4 weeks after BCAO. On other hand, daily oral administration of F-Garlic extract caused the marked preservation of LTP induction. Moreover, parvalbumin was markedly reduced in the CA1, especially, in the stratum radiatum of BCAO+water. In contrast, BCAO+F-Garlic mitigate a significantly reduction of the parvalbumin. In summary, these results suggest that daily oral administration of F-Garlic extract can ameliorate cognitive memory deficit through the preservation of synaptic plasticity and interneurons integrity in the hippocampus in rodent model of chronic cerebral hypoperfusion.

Studies on Conservation of the Beeswax-treated Annals of Joseon Dynasty (조선왕조실록 밀랍본 보존을 위한 연구)

  • Jeong, Hye Young;Choi, Kyoung Hwa;Lee, Kye Shik;Jo, Byoung-Muk
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.44 no.6
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    • pp.70-78
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    • 2012
  • The Annals of Joseon Dynasty, which recorded governmental affairs for 472 years (AD 1392-1863) during the Joseon dynasty is an representative paper cultural heritage in Korea. Being recognised their authenticity and values they were designated as the No 151 national treasure of Korea in 1973 and registered as 'The Memories of the World' by UNESCO in 1997. Unusually, some volumes of the Annals were treated by beeswax for the permanent preservation. However, the examinations on the deterioration of the beeswax-treated volumes had been exposed the serious damage than the untreated volumes. In particular, the larger amount of beeswax were worst in deterioration condition. In order to solve these problems, NRICH(National Research Institute of Cultural Heritage) of Korea launched the investigation study for the conservation of beeswax-treated volumes of the Annals. Now then, the five study areas have been set up; 1) to understand the damaged condition of the Annals; 2) to analyse the raw materials and production techniques; 3) to test the degradation degree using the duplication samples for understanding the aging factors and mechanism; 4) to develop the conservation techniques through dewaxing and thereafter strengthening the base paper of Hanji; 5) to develop the long-term storage technique using inert gases. On the basis of outcomes from this study, NRICH will plan to establish the systematic conservation and management plan for the beeswax-treated volumes of the Annals in future.

Preservation Experiment of Hibernating Silkworm Eggs by 2 Year Cold Refrigeration (월년잠종의 2년간 냉장에 의한 보존 시험)

  • Sohn, Bong-Hee;Kang, Pil-Don;Lee, Sang-Uk;Kim, Yong-Soon
    • Journal of Sericultural and Entomological Science
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    • v.46 no.2
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    • pp.65-71
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    • 2004
  • Long term preservation experiment through refrigeration was conducted for 2 year on 300 lines of silkworm races preserved, as one method for the development of long term safe preservation technique. Experiment with 6 treatments was conducted for 680 days from July 1 st 2000 to May 1st 2002. Embryonic development was conducted to each treatment. There are no differences among treatments and races in 400 days preservation, the stage of whole embryonic development was Eul B and condition of eggs was good. In 650 days preservation experiment, differences were reveled among races and treatment, the level of whole embryonic development was Byeong A and condition of eggs was good. The order of embryonic development is European races >Tropical, Korean races >Japanese, Chinese races, thus European races showed fast embryonic development. Control(treatment A) and treatment C showed faster development than other treatments. And treatment D and F showed stable individual stage among all treatments. The test of shape characteristics and embryo which were conducted in hatching period showed 61% of high line succession possibility in average of 6 treatments. But treatment A and B showed no hatching, 3 lines of treatment C, 48 lines of treatment D, 1 line of treatment E, and 29 lines of treatment F showed slow development. And treatments D and F which showed stable embryo condition had highest possibility. The two treatments D and F showed good result among six treatments, and the preservation period of treatment D and F are 235 days and 310 days, and exposure period in $-2.5^{\circ}C$...was longer than other treatments. Numbers of hatched lines of treatment D and F are 48 and 29, and occupied 15.6% and 9.4% of all tested lines, respectively. Average hatching ratio of treatment D and F were 54.% and 71.6%, and average dead egg ratio were 12.6% and 2.4%, respectively. These results show that average ratio of hatching dead eggs in treatment D and F are higher than general line. Thus reconsideration of hatching condition on treatment D and F is needed.

Long Term Results of Radiation Therapy in Early Glottic Cancer (초기 성문암의 방사선치료: 장기 추적결과)

  • Kim, Jin-Hee;Byun, Sang-Jun
    • Radiation Oncology Journal
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    • v.27 no.1
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    • pp.29-34
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    • 2009
  • Purpose: This study was designed to evaluate long-term results in terms of failure, survival and voice preservation after radiation therapy for early glottic cancer. Materials and Methods: From February 1988 to December 2003, 70 patients with early glottic cancer were treated with radiation therapy at Keimyung University Dongsan Medical Center. Patient age distribution was from 39 to 79 years, with a median age of 62 years. All patients had squamous cell carcinoma. According to the TNM stage, 58 patients had stage I disease, 12 patients had stage II disease; 67 patients were male. The laryngeal area was irradiated with the use of bilateral opposing fields with/without a wedge filter with 6 MV photons at a total dose of $54{\sim}70.2$ Gy in $1.8{\sim}2.2$ Gy fractions over $6{\sim}8$ weeks. We delivered a median radiation dose of 60 Gy for stage I patients and a median radiation dose of 66 Gy for stage II patients. Salvage surgery was performed in patients with local recurrence. The voice preservation rate was analyzed after all treatments including salvage surgery. Follow-up periods were from 13 to 180 months, with a median follow-up period of 77.5 months. The survival rate was analyzed by the use of the Kaplan Meier method and log rank test. A comparison of two groups was performed with the use of the chi-squared test. Results: The local control rate was 98.5% (69/70). The five-year-overall survival rate was 93.9%. The five-year disease free survival rate (5YDFS) was 84.1% and the 5YDFS after radiation and salvage surgery was 92.8%. According to stage, the 5YDFS was 93.1% and 91.7% for stage I and stage II respectively. Thirteen patients (18.5%) had local failure with 24 months of median time to local failure and nine patients received salvage surgery; however, four patients were lost to follow-up after a diagnosis of recurrence. Only two patients died due to a distant metastasis at 33 months and 71 months after radiation therapy, respectively. Nine patients died due to other diseases with a median time of 73 months. There were no severe acute or chronic complications after radiation therapy. Voice preservation was ultimately achieved in 88.5% (62/70) of patients. Conclusion: We considered that radiation therapy was effective and we achieved excellent survival and voice preservation in early laryngeal cancer. The use of radiation therapy should be the first choice for the treatment of early glottic cancer.

Effects of long-term endurance exercise and Salvia miltiorrhiza vinegar on body composition and insulin resistance in high fat diet-induced obese rats (장기간의 지구성 운동과 단삼식초 섭취가 고지방식 유도 비만 흰 쥐의 신체구성과 인슐린 저항성에 미치는 영향)

  • Kim, Kijin;Jung, Su-Ryun;Ahn, Na-young;Park, Ju-sik;Ju, Young-Sik;Kim, Sung-Wook;Lee, Gyu-Ho;Kim, Mi-Yeon;Jeong, Yong-Jin
    • Food Science and Preservation
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    • v.24 no.5
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    • pp.666-672
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    • 2017
  • The purpose of this study was to examine the effects of long-term endurance exercise and salvia miltiorrhiza vinegar on body composition and insulin resistance of high-fat diet (30% carbohydrate, 50% fat and 20% protein) induced obese rats. After 8 weeks of high fat diet (50% of total calories), rats were divided into 4 groups (sedentary group, n=10; exercise group, n=10; Salvia miltiorrhiza vinegar group, n=10; exercise+Salvia miltiorrhiza vinegar group, n=10) for 8 weeks. Body weight, body composition, diet intake volume, oral glucose tolerance test, plasma total cholesterol were measured. The results showed that Salvia miltiorrhiza vinegar plus endurance exercise training for 8 weeks significantly improved body weight control, visceral fat weight, and insulin resistance. However, only Salvia miltiorrhiza vinegar treatment did not significantly improve body composition and insulin resistance. In addition, there was no additive by the combination of Salvia miltiorrhiza vinegar and endurance exercise in insulin, body fat, and total cholesterol. The reduction of body fat, glucose, insulin and cholesterol by combination was resulted from the exercise. These results suggest that Salvia miltiorrhiza vinegar has slight effect on anti-hyperglycemia and anti-obesity.

Application of Artificial Neural Network Ensemble Model Considering Long-term Climate Variability: Case Study of Dam Inflow Forecasting in Han-River Basin (장기 기후 변동성을 고려한 인공신경망 앙상블 모형 적용: 한강 유역 댐 유입량 예측을 중심으로)

  • Kim, Taereem;Joo, Kyungwon;Cho, Wanhee;Heo, Jun-Haeng
    • Journal of Wetlands Research
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    • v.21 no.spc
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    • pp.61-68
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    • 2019
  • Recently, climate indices represented by quantifying atmospheric-ocean circulation patterns have been widely used to predict hydrologic variables for considering long-term climate variability. Hydrologic forecasting models based on artificial neural networks have been developed to provide accurate and stable forecasting performance. Forecasts of hydrologic variables considering climate variability can be effectively used for long-term management of water resources and environmental preservation. Therefore, identifying significant indicators for hydrologic variables and applying forecasting models still remains as a challenge. In this study, we selected representative climate indices that have significant relationships with dam inflow time series in the Han-River basin, South Korea for applying the dam inflow forecasting model. For this purpose, the ensemble empirical mode decomposition(EEMD) method was used to identify a significance between dam inflow and climate indices and an artificial neural network(ANN) ensemble model was applied to overcome the limitation of a single ANN model. As a result, the forecasting performances showed that the mean correlation coefficient of the five dams in the training period is 0.88, and the test period is 0.68. It can be expected to come out various applications using the relationship between hydrologic variables and climate variability in South Korea.

Growth of Pulmonary Autograft in Swine, I.Feasibility of the Operation (신생돈을 이용한 이식 자가 폐동맥의 성장에 관한 연구, 제1보, 수술의 적합성)

  • 안재호
    • Journal of Chest Surgery
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    • v.28 no.10
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    • pp.885-891
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    • 1995
  • In order to test the hypothesis that the pulmonic valve, when used to replace the aortic root as a pulmonary autograft, will remain a viable anatomical structure and will grow and develop normally along with the host, we performed aortic valve replacement with the pulmonary autograft in 15 neonatal piglets. The weight of the donor was 9.3 $\pm$ 0.2 kg, the recipient 9.6 $\pm$ 0.3 kg. Measured diameters of pulmonic annulus were 14 $\pm$ 0.2 mm for autograft and 14.2 $\pm$ 0.2 mm for pulmonary artery homograft. Operation was performed under cardiopulmonary bypass with deep hypothermia [20oC at low flow perfusion [70 ml/kg/min . The mean operation time was 227 $\pm$ 10 min., bypass time 152$\pm$ 7.6 min. and aortic cross clamp time 73$\pm$ 4.6 min.. 9 piglets survived more than 12 hours. One survived 12 days and died of pneumonia and the latest one survived in good condition and sacrificed at postoperative 6th week for cardiac catheterization and pathologic examination that revealed the viability and growing of the pulmonary autograft. Currently we are able to complete the operation with good preservation of cardiac function, and our postoperative care has evolved to the extent that we are now confident enough of having an acceptable percentage of long term survivors to undertake a definite study in this regard.

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지속적인 거래관계 속에서의 관계적 규범의 측정에 관한 연구

  • 오세조;김천길;배정아
    • Journal of Distribution Research
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    • v.1 no.1
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    • pp.239-258
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    • 1996
  • This study deals with the measurement of relational norms, a safeguard for sustained exchange relationshipsim marketing channel systems. Here, the relational norm items are hypothesized to have a second-order factor model, in which mine observed items originate from the three first-order factors and the first order factors in turn originate from a second-order factor. The three first order construe comprising a higher order norm are mutuality(equity in the distribution of benefits and burdens over the course of the exchange on a long term basis), flexibility(expectation of friendly adaption for possible circumstance changes), and solidarity (the degree to which the preservation of the unique and continuing relationship is internalized by the exchange partners as being important in and of itself). 113 research questionnaires are obtained from four industries such as construction, telecommunication, iron, and electric & electronic industries, Reliability and nomological. discriminant validity are tested, and in using the confirmatory factor analysis of Lisrel 7.16, and the chi-square difference test it is tested which has a better satisfactory fit to the data, the first-order model or the second-order one. The results of this study indicates that relational norms have a second order construct. In the electric and electronic industry the model as a while has a satisfactory fit to the data and the relevant first- and second-order factor loading to the mutuality is not significant. Same Results are happened to the flexibility in the telecommunication industry and to the mutuality and flexibility in the inn industry. In sum, the model lends support to our construct, but it is difficult to apply the measurement model to all kinds of industries.

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An Experimental Study On the Properties blended with industrial by products Using Mineral Admixture (산업부산물의 혼입에 따른 콘크리트 특성의 실험적 연구)

  • Kim, Dongbaek;Jun, Kyeongbae
    • Journal of the Society of Disaster Information
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    • v.10 no.2
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    • pp.238-243
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    • 2014
  • Recently environmental pollution is serious and therefore, This study aims at reviewing individual mixing ratio and engineering characteristics of concrete due to mixture and mixing using fine powder and fly ash of blast furnace slag having effect on aspects of environmental preservation and resources recycling and performance increase of the concrete, and verifying possibility of application in the field. Test results are as follows: 1)As mixing quantity of the admixture has increased, performance of the slump has been improved, 2)As mixing quantity of the admixture has increased, there is a trend of delayed ending time, 3)As mixing quantity of admixture has increased, it has showed lower strength at short time age, however, as the age has elapsed and mixing quantity has increased, strength improvement has increased and the admixture has effect on the long term age. In this study, the characteristics and critical value of concrete contained blast furnace slag and fly ash are defined, and will be examined about the field applications.

Enhancing anti-calcification efficacy in veterinary cardiovascular surgeries: evaluating short-term ethanol's role in glutaraldehyde fixed pericardial tissues in rats

  • Kyung-Min Kim;Won-Jong Lee;Woo-Jin Kim;Chang-Hwan Moon;Jaemin Jeong;Hae-Beom Lee;Seong-Mok Jeong;Dae-Hyun Kim
    • Korean Journal of Veterinary Research
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    • v.64 no.2
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    • pp.16.1-16.9
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    • 2024
  • Autologous pericardial tissues are utilized in veterinary cardiovascular surgeries due to their accessibility and effectiveness. To enhance handling and biomechanical properties, glutaraldehyde (GA) fixation is applied. However, GA fixation can induce calcification, leading to tissue failure. This study aimed to establish an optimal rapid anti-calcification protocol by integrating ethanol treatment with the proven effective GA concentration and fixation time, facilitating application from collection to utilization. Pericardia were fixed with 0.625% GA for 20 min and subjected to ethanol treatment for 0 (group A, control), 20 (group B), and 30 minutes (group C). The treated tissues underwent mechanical test and were implanted subcutaneously in 3-week-old male rats for 7 weeks before extraction, followed by calcium analysis and histological examination via hematoxylin and eosin staining. No significant differences in mechanical properties were observed among the groups. The ethanol-treated groups (groups B and C; p < 0.05) exhibited significantly lower calcium levels than control (group A). Microscopy confirmed collagen and elastic fibers preservation, without significant immune cell variance. However, higher fibrocyte presence was noted in the ethanol-treated groups. This study presents a rapid anti-calcification protocol combining ethanol treatment with optimal GA fixation, suitable for direct surgical use of autologous tissues. Further research is necessary for long-term efficacy evaluation.