• Journal of Plant Biotechnology
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• v.33 no.2
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• pp.123-131
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• 2006

Chinese cabbage (Brassica rapa ssp. pekinesis) is one of the most important vegetable crops in korea and other East Asian countries. Cytosolic fructose-1,6-bisphospha-tase (cytFBPase) is a key enzyme in sucrose biosyn-thesis, which controls the sucrose levels as well as the productivity at plants. The Chinese cabbage cytFBPase gene, BrFBPase, encodes the 340 amino acid polypep-tide, giving a theoretical molecular weight of 37.2 kD and a isolectric point of 5.4. BrFBPase showed high sequence identity with Brassica homologs and its functional domains, such as 12,6P$_2$ binding site or active site and F6P binding site, were highly conserved in diverse sources of organisms. Although the genome of Chinese cabbage seemed to be triplicated, BrFBPase appears to be a single copy gene. The expression of BrFBPase was examined at transcript and protein levels under various conditions. BrFBPase expression was observed only in photosynthetic source tissue, not in sink tissue. The expression was slightly higher during the day than at night, and it showed a diurnal cycle with circadian rhythmicity. Short-term exposure to low temperature inhibited the expression of the BrFBPase, while long-term exposure increased the expression, supporting that sugar levels are high in late autumn when temperature are low.

• Korean Journal of Plant Resources
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• v.37 no.4
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• pp.423-430
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• 2024

Cymbidium (C.) spp. 'Wedding Day' was developed by the National Institute of Horticultural and Herbal Science, Rural Development Administration in 2021. This cultivar was derived from the artificial cross between C. 'Boksam Holiday' and C. 'Persicolor' in 2008. After the crossing, 112 seedlings were obtained through in vitro germination and transferred to the green house. Based on their vegetative and flowering characteristics, two lines were selected through the first selection. To confirm the stability and uniformity of the two lines, the first and second trials were conducted from 2017 to 2021. As a result, the final line with the code 'C0844-29' was selected as 'Wongyuo F1-78'. After evaluating consumer preference, the line was named as 'Wedding Day'. This hybrid is medium sized cultivar with long vase life of cut flowers and more than 11 white flowers per stalk. It has 63.2 cm of erect stalks which are suitable for cut flower. The leaves are 65.8 cm long and located lower than the flowers, providing appropriate shape for viewing the flowers. It starts flowering from late January. It has sufficient proliferation ability to enable mass proliferation for commercial use (Registration No. 9523).

• Korean Journal of Agricultural and Forest Meteorology
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• v.25 no.1
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• pp.37-47
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• 2023

The black cutworm, Agrotis ipsilon (Hufnagel), is an important crop pest worldwide that feeds more than 80 plant species including cabbage, potato, maize, wheat and bean, and this moth is a typical pest attacking underground parts of crops. It has been known in farm booklets that the larvae of A. ipsilon overwinter in the soil in Korea, but no definitive data exist yet. This study was conducted to evaluate that the specific appearance time of A. ipsilon observed actually in the field could be explained when we assumed that this pest overwinters in a form of larvae or pupae. Degree day-based phenology models were applied for tracking forward or backward to find the predicted developmental stage which developed at a specific stage found in the field. As a result of the analysis, it was confirmed that an initial population could be established in a group that does not overwinter as larvae or pupae in Korea. In other words, the appearance of adults in early March to April could not be explained by the presence of domestic overwintering populations. Populations that overwinter as larvae or pupae in Korea were able to emerge as adults in June to July at the earliest. Therefore, the group of adults appearing in early spring is highly likely to be a population that migrated from outside Korea. Taken together, it was estimated that the colony of A. ipsilon in Korea would be formed by a mixture of a migrant population through long-distance migration and a overwintering population.

• Journal of the Korean Society of Propulsion Engineers
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• v.23 no.4
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• pp.92-97
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• 2019

A study was conducted to improve the adhesion of propellant, liner,and insulation. Insulation was shown to be more advantageous in improving the adhesion when a barrier coat was applied compared to a bare insulation layer. It was confirmed that the adhesion strength between the insulation and the propellant improves as the thickness of the liner coating increases. The liner was cured for 24 h. If the liner is cured for a long time, it will adversely affect adhesion. Adhesion is also improved when a bonding agent is applied. As the bonding agent content increases, the adhesion improves. There is a change in the adhesive strength depending on the type of bonding agent used. HX-868 shows slightly more improved adhesion than HX-752.

• Molecules and Cells
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• v.41 no.7
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• pp.665-675
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• 2018

Rice is a facultative short-day (SD) plant in which flowering is induced under SD conditions or by other environmental factors and internal genetic programs. Overexpression of Histone Deacetylase 701 (HDT701) accelerates flowering in hybrid rice. In this study, mutants defective in HDT701 flowered late under both SD and long-day conditions. Expression levels of florigens Heading date 3a (Hd3a) and Rice Flowering Locus T1 (RFT1), and their immediate upstream floral activator Early heading date 1 (Ehd1), were significantly decreased in the hdt701 mutants, indicating that HDT701 functions upstream of Ehd1 in controlling flowering time. Transcript levels of OsINDETERMINATE SPIKELET 1 (OsIDS1), an upstream repressor of Ehd1, were significantly increased in the mutants while those of OsGI and Hd1 were reduced. Chromatin-immunoprecipitation assays revealed that HDT701 directly binds to the promoter region of OsIDS1. These results suggest that HDT701 induces flowering by suppressing OsIDS1.

• Journal of Plant Biotechnology
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• v.50
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• pp.248-254
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• 2023

This study was conducted to establish an efficient plant regeneration system in 'Dayu', a Korean variety of Perilla frutescens developed for seed oil production, in conjunction with the previously studied variety 'Namcheon'. The healthiest callus was formed on the hypocotyl explants cultured on a medium containing 0.1 mg/L NAA and 0.5 mg/L BA, outperforming the leaf and cotyledon samples. In both dark and long-day conditions, Dayu consistently exhibited significantly higher shoot regeneration rates compared with Namcheon. The highest shoot regeneration rates in Dayu were observed from the hypocotyl explants cultured on 0.1 mg/L NAA and 0.5 mg/L BA media, with shoot regeneration rates of 84.4% and 86.7% under dark and long-day conditions, respectively. Various combinations of plant growth regulators were tested to establish the optimal shoot regeneration conditions for Dayu hypocotyl explants. The results demonstrated that the highest shoot regeneration rate (90%) was achieved when 0.5 mg/L of BA was added to the medium without NAA. Among the regenerated shoots, 70.5% were normal plants, while 19.3% were abnormal. The addition of NAA or an increase in its concentration led to a higher occurrence of abnormal plants. After the regenerated shoots were transferred to 1/2 MS medium, roots were observed within 10-15 days. By day 30, they had developed into complete plants. The results obtained from the regeneration experiments with the perilla variety Dayu can valuably inform molecular breeding reliant on transformation techniques such as genome-editing and genetic modification technology.

• Journal of Bio-Environment Control
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• v.20 no.3
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• pp.221-226
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• 2011

The study was carried out to investigate the effect of MA packaging materials on quality and storability of tomato in long distance export condition. We found that the fresh weight loss in perforated film was higher than other breathable films. The fresh weight of tomato (cv 'Madison') packaged with breathable films, such as 5,000 cc, 10,000 cc, $20,000cc/m^2{\cdot}day{\cdot}atm$ oxygen permeable films, reduced less than 0.6%, but perforated film that already being used for packaging horticultural crops showed 1.4% fresh weight loss during $5^{\circ}C$ storage for 20 days and then $20^{\circ}C$ storage for last 5 days with 85% relative humidity. The carbon dioxide and oxygen concentration in tomato packages showed proper level for MA storage in $20,000cc/m^2{\cdot}day{\cdot}atm$ $O_2$ breathable film treatment at $5^{\circ}C$ storage. Although at $20^{\circ}C$ storage, the carbon dioxide concentration increased sharply, the oxygen concentration decreased remarkably, the change of these gases concentration was the lowest in 20,000 cc film treatment. The ethylene concentration was sharply increased at $20^{\circ}C$ from $21^{st}$ day to $25^{th}$ day after $5^{\circ}C$ storage for 20 day, and the concentration was lower in 20,000 cc film treatment among the breathable film treatments. Until 20 days, at $5^{\circ}C$ storage all treatments did not exceed the marketability. However, the last 5 days during at $20^{\circ}C$, the fruit appeared fungal rots and the quality rapidly decreased. The $20,000cc/m^2{\cdot}day{\cdot}atm$ $O_2$ permeability treated tomato performed higher firmness (9.56 N), vitamin C (16.31 mg/100 gFW), and soluble solids ($7^{\circ}Brix$) than other breathable films at final storage day. The results suggest that the $20,000cc/m^2{\cdot}day{\cdot}atm$ $O_2$ permeable film treatment of tomato (cv 'Madison') performed the highest quality and storability of tomato for long distance exporting.

• Korean Journal of Breeding Science
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• v.43 no.6
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• pp.568-571
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• 2011

A new poinsettia cultivar 'Green Dream' was bred by the National Institute of Horticultural and Herbal Science (NIHHS) in 2009. A cross was made between 'Lemon Snow', a variety with dark green leaves and light yellow-green bracts, and 'Freedom Rose', a variety with vigorously growing deep pink bracts in 2006. 'Green Dream' was finally selected in 2009 after the investigation of the growth and flowering characteristics from 2007 to 2009. 'Green Dream' has light yellow-green ovate bracts and medium intensity of rugosity between bract veins. Leaf blade is ovate and dark green. Petiole length is long, and stem color is greenish. 'Green Dream' has medium free-branching ability and long plant height. 'Green Dream's bracts and transitional leaves are fully colored 7 weeks after short day commencement.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.4
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• pp.303-309
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• 2006

The study examined the effects of $CO_2$ enrichment on growth of soybean (Glycine max). Two soybean varieties were used, Taekwang and Cheongja. The plants were grown in growth chambers with a 12-h photoperiod and a day/night temperature of $28/21^{\circ}C$ at the seedling stage and $30/23^{\circ}C$ from the flowering stage. The plants were exposed to the two elevated $CO_2$ levels of 500 and 700 ppm and the ambient level of 350 ppm. Results of the experiment showed that at the second-node trifoliate stage of the two varieties, the elevated $CO_2$ increased plant height, leaf area and dry weight. The elevated $CO_2$ also raised the photosynthetic rate of soybean as compared to the ambient level. From the beginning bloom stage to the full maturity stage of the two varieties, the elevated $CO_2$ increased plant height, leaf area, seed weight and photosynthetic rate. The stomatal conductance and transpiration rate decreased on long days relative to short days of treatment. Through the entire stages, the elevated $CO_2$ increased the water use efficiency of soybean plants because stomatal conductance and transpiration rate decreased at the elevated $CO_2$ levels relative to the ambient level.

• Journal of the Korea institute for structural maintenance and inspection
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• v.26 no.5
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• pp.112-118
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• 2022

In this study, the diffusion characteristics were evaluated using the concrete mix design of nuclear safety-related structures. Among the concrete structures related to nuclear power safety, we selected the composition of intake and drainage structures that are immersed in seawater or located on the tidal platform and evaluated the chloride ion permeation resistance by compressive strength and electrical conductivity and the diffusion characteristics by immersion in salt water. analyzed. Compressive strength was measured on the 1st, 7th, 14th, 28th, 56th, and 91st days until the 91st day, which is the design standard strength of the nuclear power plant concrete structure, and chloride ion permeation resistance was evaluated on the 28th and 91st. After immersing the 28-day concrete specimens in salt water for 28 days, the diffusion coefficient was derived by collecting samples at different depths and analyzing the amount of chloride. As a result, it was found that after 28 days, the long-term strength enhancement effect of the nuclear power plant concrete mix with 20% fly ash replacement was higher than that of concrete using 100% ordinary Portland cement. It was also found that the nuclear power plant concrete mix has higher chloride ion permeation resistance, lower diffusion coefficient, and higher resistance to salt damage than the concrete mix using 100% ordinary Portland cement.