• 한국작물학회지
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• 제46권1호
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• pp.35-39
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• 2001

It is important to accelerate rooting and establishment of seedlings after transplanting for higher dry matter production and tuber yield in sweet potato cultivation. Therefore, this study was conducted to obtain some information about the effects of vine-cutting holding on rooting and plant growth after transplanting. Vines were cut 20-25cm long and stored for 1, 3, 5, 7 or 9 days in a shade room at 20$\pm$0.5$^{\circ}C$, RH 68$\pm$,/TEX>1%. Peroxidase activity in the vine cuttings increased as the holding period increased and the highest catalase activity was detected in the 5-day holding. Length of main vine, number of branches and nodes on main vine also increased in the 5-day holding. There was little difference in the diameter of main vine among the various holding periods. The data obtained indicate that transplanting of vine cuttings after 5-day holding could promote rooting and vine growth.

• 한국자원식물학회지
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• 제28권1호
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• pp.135-144
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• 2015

The Korean daily intake of vegetable oils has increased about 2.5-fold from 17 g/day to 46 g/day for the last several decades. Perilla (Perilla frutescens var. frutescens) has been cultivated in Korea for a long time as a dietary oil seed which has the highest content of ${\alpha}$-linolenic acid, accounting for nearly 60%. It is known that the main role of ALA is as a precursor to the longer-chain ${\omega}-3$, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), the metabolic products of ${\alpha}$-linolenic acid (ALA, ${\omega}-3$). Dietary ${\omega}-3$ fatty acids reduce inflammation and the risk of chronic diseases such as heart disease, cancer, and arthritis, but they also may act as functional components for cognitive and behavioral function. Thus, ${\alpha}$-linolenic acid is one of the essential nutrients in modern dietary patterns in which much linoleic acid is consumed. Nevertheless, perilla oil, rich in ${\alpha}$-linolenic acid, can be easily oxidized, giving rise to controversies with respect to shelf life, the deterioration of the product's commercial value, and further related toxicity. Recent research using genetic modifications has tried to develop new plant oil seeds that balance the ratio of ${\omega}-6/{\omega}-3$ fatty acids. Such trials could be a strategy for improving an easily oxidizable property of perilla oil due to high ${\alpha}$-linolenic acid. Alternatively, appropriate application of antioxidant to the oil can be considerable.

• 한국작물학회지
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• 제46권4호
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• pp.317-320
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• 2001

Sweet com seeds deteriorate faster due to low starch content than field com seeds when stored for a long tenn. This study had been conducted to observe the seed deterioration of four different sweet corns in a long tenn storage conditions in room temperature. Four kinds of sweet com genes (sh2, bt, su, and se) were harvested from 15 days to 50 days after silking with 5-day intervals. These seeds were stored in the room temperature and tested for germination percentages from 3 months to 18 months period with 3-month interval. su seeds germinated better than other types of gene. Hybrid Mecca which is sh2 gene germinated better when stored for 3 months to 18 months. For all genes, mean regression equations in relation to storage periods showed linear responses. For regression equation, the slope of sh2 gene was lower than that of su gene. The highest slope value was observed in bt gene showing faster deterioration rate. The rate at which seed deteriorates seems to be affected by the date at which it was harvested. The seeds that were harvested at the optimum time deteriorated more slowly than those which were not.

• Food Science and Biotechnology
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• 제18권1호
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• pp.234-238
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• 2009

Effects of ginseng on in vivo antioxidant capacities with age were studied in rats. All rats were reared in the conventional system. Ginseng-treated rats were supplied with ginseng water extracts (25 mg/kg/day) continuously from 6 weeks of age to spontaneous death. None of the rats showed any discernible adverse effects of treatment with ginseng-containing water. There was no significant difference in body weight (BW) gains with age between treated and control groups. However, ginseng extracts did cause a decrease in the level of serum low density lipoprotein (LDL)-cholesterol, glucose, and thiobarbituric acid reactive substances (TBARS) in the treated rats. The activities of superoxide dismutase (SOD), catalase, and glutathione peroxidase in liver cytosol decreased with age in the control group. However, these enzyme activities were well maintained in the ginseng-treated rats and, especially, catalase and glutathione peroxidase activities were consistently higher than in control rats. The levels of total sulfhydryl group (T-SH) and glutathione reductase (GR) were unchanged, and glutathione-s-transferase (GST) activity gradually decreased with age in both groups. There were no differences in T-SH, GR, or GST between the control and treatment groups. These results indicate that long-term administration of ginseng retards age-related deterioration in some biochemical parameters such as cholesterol, glucose, and lactate dehydrogenase in serum and it has an enhancing effect on antioxidant capacity in the liver.

• Membrane and Water Treatment
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• 제9권6호
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• pp.455-462
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• 2018

The measurement and monitoring of the biochemical oxygen demand (BOD) play an important role in the planning and operation of wastewater treatment plants. The most basic method for determining biochemical oxygen demand is direct measurement. However, this method is both expensive and takes a long time. A five-day period is required to determine the biochemical oxygen demand. This study has been carried out in a wastewater treatment plant in Turkey (Hurma WWTP) in order to estimate the biochemical oxygen demand a shorter time and with a lower cost. Estimation was performed using artificial neural network (ANN) method. There are three different methods in the training of artificial neural networks, respectively, multi-layered (ML-ANN), teaching learning based algorithm (TLBO-ANN) and artificial bee colony algorithm (ABC-ANN). The input flow (Q), wastewater temperature (t), pH, chemical oxygen demand (COD), suspended sediment (SS), total phosphorus (tP), total nitrogen (tN), and electrical conductivity of wastewater (EC) are used as the input parameters to estimate the BOD. The root mean squared error (RMSE) and the mean absolute error (MAE) values were used in evaluating performance criteria for each model. As a result of the general evaluation, the ML-ANN method provided the best estimation results both training and test series with 0.8924 and 0.8442 determination coefficient, respectively.

• 한국식품영양학회지
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• 제21권3호
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• pp.269-274
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• 2008

Plants have long been used as a food source in Korea. In this study, we investigated the combined immunomodulative effects of a water extract mixture of(pine needles, Sedum sarmentosum Bunge, hijkiaorme, buckwheat and Peril a leaves) on Balb/c mice $7{\sim}8$ weeks old. The mice were fed a chow diet ad libitum and the plant extract was orally administered every other day for four weeks at two different concentrations(50 and 500 mg/kg BW). After preparing the single-cell suspension, splenocyte proliferation was determined by the MTT(3-[4,5-di-methylthiazol-2-y]-2,5-diphenyl terazolium bromide) assay. After 48hrs of incubation with the mitogens(ConA or LPS) splenocyte from the mice groups administered 50 and 500 mg/kg BW of the plant extract showed a significant increased in proliferation compared to the control group. A hemolytic plague forming cell assay was used to indicate antibody production against sheep red blood cells(SRBC). The number of antibody-secreting cells T-dependent antigen. The result of this study suggest that supplementation with this plant extract may regulate immune function by increasing splenocyte proliferation and the number of plaque forming cells.

• 식물조직배양학회지
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• 제26권4호
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• pp.229-233
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• 1999

본 연구에서는 낙엽송 배발생조직의 장기저장을 위한 가능성에 대하여 조사하였다. 0.4M혹은 20% PEG로 24시간 전 처리하여 -0.33$^{\circ}C$/min의 동결온도 하강율로 초저온시켰을 때 높은 조직생중량을 보인 반면 -0.5 혹은 -1.$0^{\circ}C$/min의 경우 초저온 후 조직의 생중량이 저조한 것으로 나타났다. 1, 7, 및 28일간 초저온보존된 조직을 회수하여 재생장시킨 다음 PCR을 이용하여 변이분석을 한 결과 초저온보존 기간에 관계없이 DNA변이는 전혀 나타나지 않았다. 초저온보존된 배발생 조직으로부터 체세포배 유도 및 식물체 재분화를 유도할 수 `있었다.

• BMB Reports
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• 제40권6호
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• pp.1083-1089
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• 2007

The HAP complex has been found in many eukaryotic organisms. HAP recognizes the CCAAT box present in the promoters of 30% of all eukaryotic genes. The HAP complex consists of three subunits - HAP2, HAP3 and HAP5. In this paper, we report the biological function of the AtHAP3b gene that encodes one of the HAP3 subunits in Arabidopsis. Compared with wild-type plants, hap3b-1 and hap3b-2 mutants exhibited a delayed flowering time under long-day photoperiod conditions. Moreover, the transcription levels of FT were substantially lower in the mutants than in the wild-type plants. These results imply that AtHAP3b may function in the control of flowering time by regulating the expression of FT in Arabidopsis. In a subsequent study, AtHAP3b was found to be induced by osmotic stress. Under osmotic stress conditions, the hap3b- 1 and hap3b-2 mutants flowered considerably later than the wild-type plants. These results suggest that the AtHAP3b gene plays more important roles in the control of flowering under osmotic stress in Arabidopsis.

• 한국작물학회지
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• 제48권6호
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• pp.490-494
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• 2003

1) 들깨 속의 개화일수는 파종기 및 품종에 따라 큰 변이를 보였다. 5월 1일 이후 파종기가 지연됨에 따라 품종간 차이가 적어지는 경향이었고 8월 1일 파종기에서 품종간 5일로 가장 적은 개화일수 차이를 보였다. 2) 들깨 속은 파종기 및 품종에 따라 개화반응이 다양하였다. 1월 1일 파종 및 5월 1일 이후 파종기에서는 영양생장에서 생식생장으로 정상적인 개화 반응을 보였으나, 2월에서 4월까지 파종한 경우에는 영양생장\$\longrightarrow$생식생장\$\longrightarrow$영양생장+생식생장\$\longrightarrow$근영양생장\$\longrightarrow$생식생장, 영양생장\$\longrightarrow$영양생장+생식생장\$\longrightarrow$영양생장\$\longrightarrow$생식생장, 상위분지는 영양생장만 하고 하위분지는 영양생장과 생식생장을 동시에 하는 등 영양생장과 생식생장이 가역 적으로 변화하였다. 3)들깨의 화성자극 이동방향과 분열조직에 있는 엽수에 따른 반응을 조사하기 위하여 처리한 결과 단일 처리된 부위만 개화반응을 보여 들깨에 있어 화성자극은 이동하지 않는 것으로 도꼬마리 등 다른 식물과는 상이하였다. 4)단일처리 기간에 따른 개화일수 단축은 9월 상순 이전에 개화하는 품종은 8일 처리로 효과가 있었으나 9월 하순에 개화하는 품종은 14일 처리에서 유의하게 단축되었다.

• 생물환경조절학회지
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• 제12권1호
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• pp.1-6
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• 2003

본 실험은 정식 후 단일처리전 장일처리 기간에 따른 온실재배 스프레이 절화국 Reagan Improved품종의 건물생산과 절화품질에 미치는 영향을 구명하기 위하여 네덜란드 Wageningen 대학의 유리온실 실험포에서 수행하였다. 정식 13-14일전에 피트블록에 삽목한 묘를 단위면적당 64줄기로 2000년 9월 6, 13, 20일에 각각 정식하였고, 2000년 9월 27일 모든 처리구에 단일처리를 실시하였다. 정식에서 최종조사일까지 장일처리 기간 3주 처리구에서는 84일이었고, 2주 처리구에서는 77일, 1주 처리구에서는 70일, 단일처리 후에 최종수확일까지는 63일이 소요되었다. 개화시기는 모든 처리구에서 동일하였다. 3주 장일처리구에서의 주당 꽃송이수는 1주 장일처리구에 비해 2개 더 많았고, 꽃의 생체중 및 건물중은 각각 식물 개체당 4g과 0.4g더 무거웠지만, 각 처리구별 꽃의 품질에는 큰 차이가 없었다. 최종건물생산량 ($g{\cdot}m^{-2}$ )은 3주 장일처리구에서 높고, 1주 장일처리구에서 가장 낮게 나타났다. 본 실험에서 단일처리 전 장일기간은 단일처리 후 개화시기에 영향을 주지 않았으며 꽃의 품질면에서도 큰 차이가 없었다. 반면 초장과 생체중은 장일 조건이 길수록 높았게 나타났으며, 누적 건물중도 3주 장일처리구에서 가장 높은 결과를 나타냈다. 장일처리 기간은 꽃 자체의 품질에 영향은 적었지만, 개체 생체중 및 초장에 매우 큰 영향을 미쳤으므로 단일처리전 장일처리 기간은 개체품질 및 연중재배 생산 시스템에서 균일한 품질을 생산하기 위해 장일처리 기간의 조절은 고품질의 국화생산에 중요한 요인으로 생각되었다..