• 대한원격탐사학회지
• /
• 제38권6_3호
• /
• pp.1827-1836
• /
• 2022

재난은 돌발적으로 발생하여 예측하기가 쉽지 않고 그 규모도 과거에 비해 커지고 있어 피해가 증가하고 있으며, 하나의 재난이 2차 재난으로 발전하는 경우가 많다. 재난관리의 4가지 단계 중 응급상황이 발생하는 대응단계에서 행해지는 수색과 구조 과정에서, 현장에 투입되는 인원들은 많은 위험을 감수하고 현장에 투입되고 있다. 이러한 점에서 로봇은 재난현장의 초기 대응과정에서 인명 및 재산의 피해를 줄일 수 있는 가능성이 높은 기술이다. 또한, Light Detection And Ranging (LiDAR)는 레이저를 이용하여 비교적 넓은 범위의 3차원 정보를 획득하고 정확도 및 정밀도가 높아 재난 현장의 특징을 생각할 때 매우 유용한 센서이다. 이에 본 연구에서는 로봇이 재난 현장에서 활용될 수 있도록 LiDAR와 Inertial Measurement Unit (IMU) 센서에 실시간 모니터링을 위한 컴퓨팅 보드를 결합하여 하나의 다중센서모듈 및 조사로봇 맞춤형 Simultaneous Localization and Mapping (SLAM) 알고리즘을 개발하였다. 다중센서모듈이 재난 현장에서 최적의 정확도를 유지할 수 있도록 조사로봇에 안정적으로 탑재하는 방안에 대해 연구하였고, 모듈의 성능을 확인하기 위해 재난건축물 실내에서 SLAM 맵핑을 수행하여 다양한 SLAM알고리즘과 거리 비교를 수행하였다. 그 결과, 본 연구에서 개발한 PackSLAM이 낮은 오차를 나타내어 활용 가능성을 보였다. 향후 재난현장에서의 적용성을 더욱 높이기 위해 장애물이 많은 험지환경을 구축하여 다양한 실험을 수행할 예정이다.

• 디자인학연구
• /
• 제15권4호
• /
• pp.57-66
• /
• 2002

지방화시대에 살고있는 현실에서 현재의 도시는 새로운 상황을 맞이하고 있다. 도시의 정체성은 한 도시가 그 도시다울 뿐 아니라 다른 도시와 다른 특이성과 우월성이 있어야만 비로소 구체적으로 나타난다. 이러한 도시의 특성은 도시 자체가 가지고 있는 속성이라기 보다는 그 도시에 대란 이미지라고 할 수 있다. 도시의 정체성 확립을 나타내는 도시CI(City Identity)는 도시환경의 정비 및 계획과 시장산업의 장려 등을 통한 지역활성화로 구분된다. 즉, 자신들 지역의 미래상을 명확하게 하여 지역이미지 향상사업, 신규시책의 실시 등에 의한 지역의 변혁을 계획한다. 따라서 지역의 변혁사업을 주민에게 알려 새로운 도시 만들기에 이해와 능동적인 참여를 유도하는 것이다. 지역적 특성에 대한 고려천이 획일적인 도시환경 및 도시이미지 등의 부분은 새롭게 개선되어야 할 것이다. 따라서 현재의 도시상황을 극복하고, 그 도시만의 아이덴티티와 커뮤니케이션 전략을 통한 차별화는 도시CI의 정립을 통해 이루어질 수 있다. 이에 본 연구에서는 도시 이미지 차별화를 위한 도시CI추진 방안을 제시 하고자 한다. 첫째, 도시의 제반여건 및 기반의 조성을 목표로 하여 도시이미지 자원조사를 통한 자원을 발굴하고, 둘째, 시민 스스로 도시를 컨트롤하고 비젼을 갖고 구상해가기 위한 동기를 부여한다. 셋째, 도시디자인의 통일이나 도시심벌존의 개발, 도시 가로환경 디자인개선, 혹은 도시의 정보발신력을 강화, 확대해가기 위한 이벤트나 지역특산품 개발사업 등을 전개 해 나가며, 넷째, 행정조직의 활성화, 시민의식의 활성화, 도시이미지의 개성화라는 목표를 위해 구축된 커뮤니케이션 디자인 시스템의 적용한다. 이와 같이 요소들의 유기적 결합이 이루어지고, 그 안에서 지역문화의 진홍과 통합, 도시구조와 도시시설기능의 개발, 시민의식의 활성화 등이 형성되도록 하는 노력도 뒤따라야 할 것이다. CI(City Identity)는 도시의 개성을 특화시켜 살기좋은 정주환경을 조성함으로써 질높은 삶의 터전을 구축하고, 보다 나은 도시환경의 창출을 위한 다양한 활동이 요구된다.

• 감성과학
• /
• 제20권3호
• /
• pp.13-22
• /
• 2017

감성은 의사 결정, 지각 등에 직접적으로 영향을 미치며 인간의 삶에서 중요한 역할을 한다. 본 논문에서는 고각성 부정 감성의 편리하고 정확한 인식에 있어서, 생체신호를 이용한 분석 알고리즘을 설계하고자 한다. 이를 위해 본 연구에서는 보통 / 공포 감성 유발 영상을 이용하여 두 감성을 유도한 후, 생체신호 중 간단한 피부전도도 신호를 측정하였다. 측정된 피부전도도에 대해 Tonic 성분과 Phasic 성분으로 분해하고 감성 자극과 관련된 Phasic 성분을 더 상세하게 SCVSR, SCSR로 분해하여 각 성분의 주요한 특징들을 추출함으로써, 정확한 분석을 하기 위해 기존의 사용된 방법이 아닌 우수한 시간-주파수 지역화 특성을 가진 이산 웨이브렛 변환을 사용하였다. 추출된 특징들은 Phasic 성분의 최댓값, Phasic 성분의 진폭, SCVSR의 영교차율, SCSR의 영교차율이다. 분석 결과, 4가지 특징들 모두 고각성 부정 감성의 경우가 저각성 보통 감성의 경우보다 더 높은 값을 나타내고, 기존의 분석 방법보다 통계적으로 두 감정 사이의 더 유의미한 차이를 확인할 수 있었다. 이에 따라 본 연구의 결과는 피부전도도가 고각성 부정 감성 측정에 대해 유용한 지표라는 것을 확인하였으며, 향후 피부전도도를 이용한 실시간 부정 감성 평가 시스템 개발에 기여할 수 있을 것을 나타낸다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제23권4호
• /
• pp.430-436
• /
• 2010

The objective of the present studies was to develop and validate a system for isolation, purification and extended culture of pigment-producing cells in alpaca skin (melanocytes) responsible for coat color and to determine the effect of alpha melanocyte stimulating hormone treatment on mRNA expression for the melanocortin 1 receptor, a key gene involved in coat color regulation in other species. Skin punch biopsies were harvested from the dorsal region of 1-3 yr old alpacas and three different enzyme digestion methods were evaluated for effects on yield of viable cells and attachment in vitro. Greatest cell yields and attachment were obtained following dispersion with dispase II relative to trypsin and trypsin-EDTA treatment. Culture of cells in medium supplemented with basic fibroblast growth factor, bovine pituitary extract, hydrocortisone, insulin, 12-O-tetradecanolphorbol-13-acetate and cholera toxin yielded highly pure populations of melanocytes by passage 3 as confirmed by detection of tyrosinase activity and immunocytochemical localization of melanocyte markers including tyrosinase, S-100 and micropthalmia-associated transcription factor. Abundance of mRNA for tyrosinase, a key enzyme in melanocyte pigment production, was maintained through 10 passages showing preservation of melanocyte phenotypic characteristics with extended culture. To determine hormonal responsiveness of cultured melanocytes and investigate regulation of melanocortin 1 receptor expression, cultured melanocytes were treated with increasing concentrations of ${\alpha}$-melanocyte stimulating hormone. Treatment with ${\alpha}$-melanocyte stimulating hormone increased melanocortin receptor 1 mRNA in a dose dependent fashion. The results demonstrated culture of pure populations of alpaca melanocytes to 10 passages and illustrate the potential utility of such cells for studies of intrinsic and extrinsic regulation of genes controlling pigmentation and coat color in fiber-producing species.

• Parasites, Hosts and Diseases
• /
• 제56권5호
• /
• pp.409-418
• /
• 2018

Acanthamoeba spp. are free-living protozoa that are opportunistic pathogens for humans. Cysteine proteases of Acanthamoeba have been partially characterized, but their biochemical and functional properties are not clearly understood yet. In this study, we isolated a gene encoding cysteine protease of A. castellanii (AcCP) and its biochemical and functional properties were analyzed. Sequence analysis of AcCP suggests that this enzyme is a typical cathepsin L family cysteine protease, which shares similar structural characteristics with other cathepsin L-like enzymes. The recombinant AcCP showed enzymatic activity in acidic conditions with an optimum at pH 4.0. The recombinant enzyme effectively hydrolyzed human proteins including hemoglobin, albumin, immunoglobuins A and G, and fibronectin at acidic pH. AcCP mainly localized in lysosomal compartment and its expression was observed in both trophozoites and cysts. AcCP was also identified in cultured medium of A. castellanii. Considering to lysosomal localization, secretion or release by trophozoites and continuous expression in trophozoites and cysts, the enzyme could be a multifunctional enzyme that plays important biological functions for nutrition, development and pathogenicity of A. castellanii. These results also imply that AcCP can be a promising target for development of chemotherapeutic drug for Acanthamoeba infections.

• Reproductive and Developmental Biology
• /
• 제34권3호
• /
• pp.143-151
• /
• 2010

Pluripotency of human embryonic stem cell (hESC) is one of the most valuable ability of hESCs for applying cell therapy field, but also showing side effect, for example teratoma formation. When transplant multipotent stem cell, such as mesnchymal stem cell (MSC) which retains similar differentiation ability, they do not form teratoma in vivo, but there exist limitation of cellular source supply. Accordingly, differentiation of hESC into MSC will be promising cellular source with strong points of both hESC and MSC line. In this study, we described the derivation of MSC like cell population from feeder free cultured hESC (hESC-MSC) using direct differentiation system. Cells population, hESC-MSC and bone marrow derived MSC (BM-MSC) retained similar characteristics in vitro, such as morphology, MSC specific marker expression and differentiation capacity. At the point of differentiation of both cell populations, differentiation rate was slower in hESC-MSC than BM-MSC. As these reason, to verify differentially expressed molecular condition of both cell population which bring out different differentiation rate, we compare the molecular condition of hESC-MSC and BM-MSC using 2-D proteomic analysis tool. In the proteomic analysis, we identified 49 differentially expressed proteins in hESC-MSC and BM-MSC, and they involved in different biological process such as positive regulation of molecular function, biological process, cellular metabolic process, nitrogen compound metabolic process, macromolecule metabolic process, metabolic process, molecular function, and positive regulation of molecular function and regulation of ubiquitin protein ligase activity during mitotic cell cycle, cellular response to stress, and RNA localization. As the related function of differentially expressed proteins, we sought to these proteins were key regulators which contribute to their differentiation rate, developmental process and cell proliferation. Our results suggest that the expressions of these proteins between the hESC-MSC and BM-MSC, could give to us further evidence for hESC differentiation into the mesenchymal stem cell is associated with a differentiation factor. As the initial step to understand fundamental difference of hESC-MSC and BM-MSC, we sought to investigate different protein expression profile. And the grafting of hESC differentiation into MSC and their comparative proteomic analysis will be positively contribute to cell therapy without cellular source limitation, also with exact background of their molecular condition.

• Journal of Korean Neurosurgical Society
• /
• 제29권8호
• /
• pp.985-994
• /
• 2000

Objective : There are some advantages of trigeminal evoked potential(TEP) recording compared to other somatosensory evoked potential(SSEP) recordings. The trigeminal sensory pathway has a pure sensory nerve branch, a broader receptive field in cerebral cortex, and a shorter pathway. Despite these advantages, there is little agreement as to what constitutes a normal response and what wave forms truly characterize the intraoperative TEP. This study presents the normative data of TEP recorded on the epidural surface of the rat with a platinum ball electrode. Materials & Methods : Under general anesthesia with urethane, the adult Sprague-Dawley male rats(300-350g) were given electrical stimulation with two stainless steel electrodes which were inserted into the subcutaneous layer of the area around whiskers. A reference electrode was positioned in the temporalis muscle ipsilateral to the recording site. Results : TEPs were recorded in the Par I area of somatosensory cortex and recorded most apparently on the point of 2mm posterior from the bregma and 6mm lateral from the midline. The typical wave form consisted of 5 peaks (N1-P1-N2-P2-N3 according to emerging order, upward negativity). Each latency to corresponding peaks was not influenced by the different intensities of stimulation, especially from 1 to 5mA. Average latencies of 5 peaks were in the following order ; 7.7, 11.1, 15, 22.3, 29.4ms. There was also no significant difference between latencies before and after administration of muscle relaxant(pancuronium). For the electrophysiological localization of recorded waves, the action potential of a single unit was recorded with glass microelectrode(filled with 2M NaCl, $3-5M{\Omega}$) in the thalamus of rat. A sharp wave was recorded in the VPM nucleus, in which the latency was shorter than that of N1. This suggests that all 5 peaks were generated by neural activities in the suprathalamic pathway. Conclusion : In terms of recording near-field potentials, our data also suggests that TEP in the rat may be superior to other SSEPs. In overall, these results may afford normative data for the studies of supratentorial lesions such as hydrocephalus or cerebral ischemia which can have an influence on near-field potentials.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권23호
• /
• pp.10451-10456
• /
• 2015

Background: We defined melanoma distribution in a large series of Turkish patients and evaluated the prognostic parameters of melanomas. Materials and Methods: A total of 1574 patients' data was retrospectively collected at 18 centers in Turkey. Demographic characteristics were questioned and noted. Prognostic parametres were evaluated based on sentinel lymph node involvement. Results: Mean age was 56.7 (4-99) years. While 844 (53.6%) cases were male, 730 (46.4%) cases were female. One thousand four hundred forty-seven (92%) cases were invasive melanoma and 127 (8%) cases were in-situ melanoma. The most common histopathological form was the superficial spreading melanoma (SSM) which was found in 549 patients (37.9%). It was followed by nodular melanoma in 379 (26.2%), acral lentiginous melanoma (ALM) in 191 (13.2%) and lentigo maligna melanoma in 132 (9.1%), respectively. On univariate analysis, lymphovascular invasion (p<0.001), tumor thickness (p<0.001), histopathological subtype (p<0.001), Clark level (p=0.001), ulceration (p<0.001), ${\geq}6/mm^2$ mitosis (p=0.005), satellite formation (p=0.001) and gender (p=0.03) were found to be associated with sentinel lymph node positivity. Regression was associated with sentinel lymph node negativity (p=0.017). According to multivariate analysis, lymphovascular invasion and tumor thickness were significant independent predictive factors of SLN positivity. Patient age, tumor localization, precursor lesions, lymphocytic infiltration and neurotropism were not related with sentinel lymph node involvement. Conclusions: In this retrospective analysis, it was found that the prevalence of SSM is at a lower rate while the prevalence of ALM is at a higher rate when compared to western countries. According to Breslow index; most of the melanoma lesions' thickness were greater than 2 mm, corresponding Clark IV. Vascular invasion and tumor thickness are the most important factors for sentinel lymph node involvement.

• 한국정보과학회논문지:소프트웨어및응용
• /
• 제33권5호
• /
• pp.481-488
• /
• 2006

부공간 투영기술(subspace projection)을 이용한 얼굴인식기술의 성능은 이들 기저영상들(basis images)의 특징과 밀접한 관련이 있다. 특히 표정변화와 같은 국부적 왜곡이나 오클루전이 있는 경우의 인식성능은 기저영상들의 특징에 의해 영향을 받게 된다. 부공간 투영기반의 얼굴인식 방법이 오클루전이나 표정변화와 같은 국부적인 왜곡발생에 강인하려면 부분국부적 표현(part-based local representation)의 기저벡터를 갖는 것이 중요하다. 본 연구에서는 국부적 왜곡과 오클루전에 강인한 효과적인 부분국부적 표현방법을 제안한다. 제안한 방법을 LS-ICA(locally salient ICA) 방법이라고 명명하였다. LS-ICA방법은 ICA 구조I의 기저영상을 구하는 과정에서 공간적인 국부성(locality)의 제약조건을 부과함으로써 부분국부적 기저영상(part-based local basis images)을 얻는 방법이다. 결과적으로 공간적으로 현저한 특징만을 포함하는 기저영상을 사용하게 되며, 이는 "Recognition by Parts"의 방법론과 유사하다. LS-ICA방법과 LNMF(Localized Non-negative Matrix Factorization)와 LFA(Local Feature Analysis)와 같은 기존의 부분 표현방법(part-based representation)들에 대해 다양한 얼굴영상 데이타베이스를 사용하여 실험한 결과, LS-ICA방법이 기존의 방법에 비하여 높은 인식성능을 보였으며, 특히 오클루전이나 국부적인 변형이 포함된 얼굴영상에서 뛰어난 인식성능을 보였다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권21호
• /
• pp.9143-9146
• /
• 2014

Background: The aim of this study was to investigate the general characteristics of patients with deep vein thrombosis (DVT) and pancreatic cancer as well as evaluate the relationship between mean platelet volume (MPV), DVT and survival. Materials and Methods: Seventy-seven patients with pancreatic cancer, who were admitted to Cukurova University Medical Faculty, Department of Medical Oncology, were enrolled in the study Results: The mean age was $59{\pm}20$. Forty-nine (63.6%) were men and 28 women (36.4%). Sixty-eight (88.3%) patients had adenocarcinoma and 9 (11.7%) had a malignant epithelial tumor. Thirty-six (46.7%) had liver metastasis at diagnosis. Twenty-six (33.8%) patients were alive, 20 (26%) were dead and in 31 (40.2%) the status was unknown. Only 14 (18.1%) patients had DVT. In 42 (54.5%) patients MPV values were normal, in 28 (36.4%) patients they were above normal, and in 7 (9.1%) patients they were below normal. There was no statistically significant difference between gender, tumour localization, chemotherapy and survival rates (p:0.56, p:0.11, p:0.21). There was no significant difference between DVT, gender, localisation, histological subtype, the presence of metastasis, stage and if the patient had been treated with chemotherapy (p:0.5, p:0.6, p:0.2, p:0.32, p:0.1, p:0.84). There was also no significant difference between MPV and DVT (p:0.57) but there was a significant difference between liver metastasis and DVT (p:0.02). Age, stage, the presence of metastasis and DVT were prognostic in pancreatic cancer patients. Conclusions: Cases of pancreatic cancer with liver metastasis should be studied more carefully as thrombosis is more common in these patients.