• 제목/요약/키워드: live data

검색결과 1,466건 처리시간 0.03초

한국 3개 지역의 결혼, 결혼년령 및 출산력에 관한 연구 (AGE AT MARRIAGE AND FERTILITY OF WOMEN IN THREE SELECTED AREAS IN KOREA, 1970)

  • 김모임
    • 대한간호학회지
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    • 제3권3호
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    • pp.1-14
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    • 1973
  • This study is designed to meet the following objectives: (1) To study attitude and behavior regarding marriage and age at marriage, (2) To learn correlates of age at marriage and to examine their relations, (3) To measure relative importance of the correlates of age at marriage, and (4) To study relations of age at marriage and family planning practice to fertility and their relative importance as correlates of fertility. The data are obtained by an independent cross-sectional survey in three study areas purposively selected to represent metropolitan. semihuman. rural population. The study population is confined to women age 17-50 as of survey. The overall response rate is 90%. Reliability of data is measured by . individual and aggregate inconsistency based upon a 15% subsample of the original interviews. The individual inconsistency (31%) is found to be high compared to the aggregate inconsistency (6%) for all 85 variables. However, the magnitude of differences between means is small, and the mean absolute shifts and proportional shifts are also small on the whole. In a word respondents did not change their answers too extremely or radically. The study populations of each study area are compared on some basic characteristics. It is found that the three study populations have more dissimilarities than similarities. The findings on seven different attitudinal positions of women toward marriage indicate that there have been tremendous changes in all study areas Iron "traditional" attitudes which have been prevalent for a long time in Korean society to "liberalized" or "modernized" attitudes. An apparent tendency is that women generally take a position of a "golden mean" attitude by not preferring either extreme of marriage attitudes. Nevertheless, the young, single, educated, and urbanite appears more "liberalized. " There has been some increase in ideal age at marriage from 1958 to 1970 for both sexes. No age group, marital status, or study area differentials in ideal age at marriage are found, the average ideal age at marriage in every sub-group being 24-25. Awareness of existing legal marriageable ages is low; only 4.4% are aware that "with parental permission: minimum age for males is 18 years and for females 16 years,"and only 3.7% are aware that "without parental permission: 27 years for males and 23 years for females." People in Korra tend to marry spouses who are in various social ways like themselves: the similarities include (a) education, occupational status of father, (c) economic status, (d) usual residence before marriage, and (e) religion. Both singulars and actual mean ages at marriage in this study confirm the trend of rising age at marriage previously established by other independent studies. The urban-rural differential in age at marriage is observed, but the differential narrows down gradually from 1935 to 1970. All socio-economic, demographic, and other variables pertaining to wife before and at first marriage, excluding (a) religion, (b) father′s of occupation, and (c) as: of menarche, are correlated with respondent's age at first marriage, whereas only three variables out of all socio-economic variables relating to husband before and at wife′s first marriage, viz., (a) education, (b) usual residence, and (c) economic level of his old home, are correlated with respondent′s age at marriage. Among socio-economic and modernity variables related to either husband or wife at the time of survey, only education and duration of residence are correlated with wife′s age at first marriage. Among the correlates of respondent′age at first marriage, education is in general the most important variable. However, it is found that wife′s education is more important than husband′s. The combined effects or the correlates studied explain no more than about 40% of variance for any of the selected groups of variables. Points which might counteract the effects of late marriage on fertility are not serious in Korea. For each of the correlates of the three fertility indices chosen for this study. namely, (a) number of living children, (b) number of live births, and (c) number of pregnancies, age at marriage is the major contributor to the variance in all age groups except the age group of 20-29 in which the index of family planning practice is the major contributor. The proportion of variability in fertility indices accounted for by the correlates is never more than 40% of the total variance in any age group. Based upon the findings from this study, it could be concluded that in the foreseeable future (a) celibate group will no! be increased to a point that would slow down population growth rate in Korea, (b) age at marriage will not increase continually, (c) although education stands out as the major contributing variable which independently explains the variation in age at marriage, it seems probable that education may not be the major variable in the near future, and (d) despite the fact found by this study that age at marriages has been the major contributor to the variance of each of the fertility indices used, family planning practice will play a more important role in the reduction of fertility in the Korean society. Therefore, factors interrupting practice of family planning must be eliminated and family planning program should be strengthened if further fertility reduction is needed.

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재택노인의 생활기능상태와 생활만족도에 관한 연구 (A Study on the Functional Status in Life and Life Satisfaction for Elderly Residing at Home - Comparing Urban and Rural Elderly -)

  • 이재면
    • 보건교육건강증진학회지
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    • 제12권2호
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    • pp.109-119
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    • 1995
  • As the population of elderly in Korea is increasing rapidly since the 1990's and will more rapidly in the 21st Century, the demand of their health care would be a great burden to health care expenditure. Then it would be necessary to contemplate the functional status and life satisfaction for elderly to make them live more independently. The objectives of this study were find out the functional status in life and life satisfaction for the elderly aged 65 or over who had resided in urban area of two Gus in Pusan and rural area of two Myuns in Haman Gun in Kyeognam Province, and to provide basic data for planning systematic health care programme. The study period was two weeks from February 6 to February 18, 1995 and the subjects were 274 elderly of which 143 were urban residents, 131 were rural residents, and the study method was by structured questionnaire. The data were analyzed with SAS/PC/sup +/ programme using Chi-square test, t-test, ANOVA, Pearson's correlation coefficients, and multiple regression analysis. The results were as follows; 1. To see the pattern of living together, those living with partners were the most common, 39.1% ; 37.8% of urban elderly lived with married sons, 32.2% of them lived with partners, but 46.6% of rural elderly lived with partners, 16.8% of them lived with married sons, which showed difference between residence(p<0.005). 2. Elderly who had jobs were 64.1% in rural residents, and 7.7% in urban residents, which showed significant difference(p<0.05). 3. The score of cognitive function of total subjects was 24.7, that of urban elderly was 23.8, and that of rural elderly was 25.7, then it was higher in rural ones and low for old-elders and those who had no jobs. 4. The score of PADL was 26.8 for urban elderly, 30.1 for rural elderly, and that of IADL was 22.2 for urban elderly, 25.6 for rural elderly, which showed higher activities of daily living for rural elderly than urban elderly(p<0.001). 5. The score of domestic performance was 21.9 for urban elderly, and 30.5 for rural elderly, which showed higher score for rural elderly(p<0.001). 6. The score of life satisfaction was 20.7 for urban elderly, 29.8 for rural elderly, then it was higher for rural elderly(p<0.01). 7. As a result of ANOVA for functional status in living by general characteristics; the score of cognitive function differed by age, job; that of PADL differed by age, job, education, and the pattern of living together, that of IADL differed by age, job, and the pattern of living together. The score of domestic role performance differed by age, job, marital status, and the pattern of living together. 8. ANOVA for life satisfaction showed that the score of life satisfaction differed by job(p<0.001) and the pattern of living together(p<0.01). 9. The correlations between functional status in living and life satisfaction showed that the higher the score of cognitive function was(r=0.39), the higher the score of activities of daily living was(r=0.50), and the higher the score of domestic role performance was(r=0.41), the higher the score of life satisfaction. 10. Stepwise multiple regression analysis for life satisfaction pointed out that residence was responsible for 39.9% of the variance. cognitive function was for 5.3%, and domestic role performance was for 1.2%.

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부산지역의 김치 염도 및 김치 염도에 대한 인식도 (The Study on the Salinity of Kimchi and Subjective Perception of Salinity in Pusan Area)

  • 문갑순;송영선;이치간;김성경;류복미;전영수
    • 한국식품조리과학회지
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    • 제13권2호
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    • pp.179-184
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    • 1997
  • 부산 지역의 김치 염도 및 김치 염도에 관한 인식도를 조사하여 김치 염도의 표준화의 기초 자료로 삼기 위해 1996년 6, 7월에 걸쳐 부산지역 4개 대학의 학부모와 1997년 1, 2월에 걸쳐 부산 여성문화회관 주부를 대상으로 김치에 대한 설문조사를 실시하고 수거한 김치의 염도를 측정하였다. 설문 조사의 결과 조사 대상자는 연령별로는 30, 40대가 가장 많았고, 학력은 고졸이, 월수입은 100~199만원 사이가 가장 많았다. 조사 대상자들의 연령과 월수입에 따른 김치 염도를 측정한 결과 월수입에 따른 김치 염도의 차이는 없었으며, 여름 김치의 경우 연령에 따른 상관성은 나타나지 않았고, 겨울김치의 경우 연령이 증가함에 따라 김치 염도가 증가하는 경향이었고, 젊을수록 저염 김치를 선호하는 것으로 나타났다. 주거주지는 부산, 경남지역이 90%이상이었고, 다수가 주거주지가 김치 담그는 방법에 영향을 미친다고 응답하였다. 김치 담그는 법의 전수자로서는 친정 어머니가 가장 많았으며, 자가 김치의 경우 비교적 일정한 염도를 나타낸다고 답하였다. 자가 김치에 대해 본인과 가족이 약간 싱겁다고 생각하는 염도는 여름김치의 경우 각각 2.46$\pm$0.24%와 2.57$\pm$0.23%였고, 적당하다고 생각하는 것은 각각 2.61$\pm$0.51%와 2.65$\pm$0.57%였으며, 짜다고 생각하는 염도는 각각 2.77$\pm$0.61%과 2.62$\pm$0.47%로써 김치 염도와 염도에 대한 인식도가 거의 일치하였다. 겨울김치의 경우 본인과 가족이 약간 싱겁다고 생각하는 염도는 각각 2.98$\pm$0.83%와 3.02$\pm$0.71%였고, 적당하다고 생각하는 것은 각각 2.82$\pm$0.49%와 2.94$\pm$0.53%이었으며, 짜다고 생각하는 염도는 각각 3.20$\pm$0.61%과 3.25$\pm$0.49%로 나타나 김치염도와 염도에 대한 인식도와는 약간 일치하지 않았다. 겨울김치 시료 186개의 평균 염도는 2.97$\pm$0.54%로 장기간 저장을 위해 3%정도의 염도를 나타내었지만, 여름김치 시료 201개에 대한 평균 염도는 2.55$\pm$0.44%로 염도가 상당히 낮은 것으로 나타났다 김치의 염도는 90%이상이 건강과 관련이 있다고 답하였고, 김치를 조금 싱겁게 해서 먹는 것이 좋다고 답한 사람이 70~80%를 나타내어 앞으로 김치 염도는 현재보다 조금 낮게 표준화하는 것이 필요할 것으로 생각된다.

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강점인식이 은퇴 후 창업의도에 미치는 영향: 직장인을 대상으로 (Effects of Strength Recognition on the Intent of Start-up after Retirement: To the Office Workers)

  • 박혜성;박우진;배병윤
    • 벤처창업연구
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    • 제15권1호
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    • pp.143-150
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    • 2020
  • 본 연구는 직장인을 대상으로 강점인식이 강점 활용을 매개로 하였을 때 창업의도에 어떤 영향을 미치는지 확인하고자 하였다. 설문기간은 2019년 1월 17일부터 3월 8일 까지 2개월간 진행했으며 이런 내용의 연구 목적 달성을 위해 전국 직장인을 대상으로 유의 표집 하였으며 설문지 300 부의 설문지를 회수 후 성실하지 못한 13부를 제외한 287 명에 대한 분석을 실시하였다. 유의 표집 된 자료는 구조방정식 모형 분석과 기술 통계분석을 활용 분석하는 과정을 거쳤으며 구조모형의 적합도와 측정모형의 적합도를 확인 변수간의 관계를 분석하는 과정을 거쳤다. 본 연구의 결과는 다음과 같이 정리 한다. 첫째 강점인식은 강점 활용에 유의한 영향을 미치는 것으로 확인 되었다. 둘째 강점 활용은 창업의도에 유의한 영향을 미치는 것으로 확인되었다. 대부분의 직장인들은 미래에 대하여 불안해하며 다양한 계획들을 세운다. 은퇴 후 살아가야 할 시간은 길어지는데 일자리와 근무 시간은 너무나 빠른 속도로 줄어들고 있고, 2017 중기 부 자료중 소상공인 실태 분석 자료에 의하면 전국 소상공인들의 월평균 영업 이익은 해가 거듭 될수록 줄어들고 있으며 창업자의 대부분은 생계형 창업으로 창업 외에 다른 선택의 여지가 없어 창업을 결심한다. 금융위기 이후 경영상 어려움을 겪는 소상공인들은 갈수록 늘어나고 직장인의 고용불안은 은퇴 후 경제적인 어려움을 겪는 이들을 창업시장의 전쟁터로 내몰고 있음에 이와 같은 연구 결과는 선제적인 본인 스스로의 강점을 인식하여 창업을 준비하는 은퇴 후의 직장인들의 창업의지에 강정인식과 강점 활용의 중요성을 증진시키는 것이 필요함을 시사한다.

왕느릅나무 추출물에 의한 비장세포 생존률 및 NK cell 세포독성 증가 (Ulmus macrocarpa Hance Water Extract Improved Splenocytes Survival and NK Cell Cytotoxicity)

  • 이성도;김덕원;이인환;이종환;현숙경;강경화;황혜진;김철민;김병우;정경태
    • 생명과학회지
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    • 제26권1호
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    • pp.109-116
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    • 2016
  • 면역에 대한 관심은 점차 증가하는 추세이며, 식물유래 천연물을 이용한 면역기능 증강에 관련된 연구 역시 활발히 진행되고 있다. 왕느릅나무 껍질은 줄기 혹은 뿌리의 껍질을 뜻하며 전통적으로 동·서양 할 것 없이 항염, 진통, 항암, 상처치료에 사용되어 왔다. 본 연구는 왕느릅나무 열수 추출물(Ulmus macrocarpa water extract, UMWE)이 면역기능에 끼치는 영향을 조사하기 위해 실시되었다. 실험은 UMWE를 농도 100 mg/kg 또는 200 mgkg로 식이한 군, UMW를 농도 100 mg/kg 또는 200 mg/kg으로 식이하면서 면역억제물질인 cyclophosphamide(CY, 120 mg/kg)를 투여한 군, CY만을 투여한 군, 아무 것도 처리하지 않은 비처리군, 총 6개 군으로 나누어 2주간 매일 식이하면서 진행하였다. 각 군에서 획득한 비장지수와 비장세포 지수를 비교하였을 때 UMWE 식이가 CY에 의한 비장세포의 감소를 완화시키는 것으로 나타났으며, in vitro 실험에서 MTT방법과 7-amino-actinomycin D 방법을 통해 비장세포의 생존을 유지하며 사멸을 지연하는 것이 확인되었다. 또한, UMWE는 YAC-1에 대한 비장 NK 세포 활성을 면역억제제 CY가 존재하는 조건에서도 정상적으로 유지시켜 면역기능 유지에 영향을 주는 것으로 나타났다.

한방건강증진센터 설립에 대한 인식 및 요구조사 (A Need Assessment on Establishment of Oriental Health Promotion Center)

  • 이향련;김귀분;조결자;신혜숙;김광주;문희자;박신애;김윤희;강현숙
    • 동서간호학연구지
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    • 제5권1호
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    • pp.90-101
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    • 2000
  • The study attempts to examine the degree of cognition and demand on health promotion center of oriental nursing. It puts unique nursing intervention using traditional health promotion connected with oriental medicine to practical use for residents' health promotion and prevention of disease. With the study design of cross-sectional descriptive study, 516 residents who live in 26 Dongs, Dongdaemoon-gu were selected. The tool of study consists of 30 questions which the study team made for the degree of cognition and demand on health promotion center of oriental nursing. Cronbach's $\alpha$ in the degree of cognition was .8028. Collection of research data had been done from October 1 to October 30, 1999 with help of Dongdaemoon-gu office after pretest from 20 residents. Collected data were analyzed into the number and percentage in the characteristic of a subject and connected with demand on the establishment of center, the mean and the standard deviation in the degree of cognition and F-tests in the difference of the degree of cognition by characteristics. The results were as follow; 1) The characteristic of subject of this study was male 50.6%, and average age was 38.5 years old and 30-39 years old occupied the highest percentage with 31.6%. The married were 71.8%, over high school graduates was 85.6%, monthly income from 500 thousand won to 2 million won was 86.1%. 50.8% was the type living with parents, children and sibling. 2) When they were sick, the institution which residents used at first was a pharmacy(69.2%) and hospital(27.5%), but oriental medicine hospital was just 1.4%. As for subjective health condition, 82.5% answered over average, and 28.7% answered that they had chronic illness such as arthritis, chronic digestion problem, hypertension and so on. As for information collection on health, mass communication(34.9%) and medically concerned people(28.1%) occupied relatively high rate. Free health diagnosis system(36.8%) and establishment of health promotion center(31.5%) among welfare programs that residents want to enjoy were high ranked. The rate using a special institution for health was 17.8%, and among these institutions, the rate using aerobic exercises, health center(7.0%) and steamed room(5.4%) was high. Besides, other institutions such as breathing at the abdomen, finger-pressure therapy, meridian massage, foot massage, and so on were being used. 3) As the average of the degree of cognition on health promotion center of oriental medicine was 2.92, the degree of cognition was medium. The description, "health promotion center of oriental medicine is necessary for health keeping of healthy people, including people who have a problem in health" showed the highest degree of cognition(3.04, ${\pm}0.64$). 4) As for the intention on using health promotion center of oriental nursing, 61.4% said "yes", "no" was just 1.4%. The services that people relatively high wanted to be served from the center were measures reducing stress(68.0%) (relaxation therapy, meditation, breathing at the abdomen and so on), acupuncture(66.5%), finger-pressure(61.6%), moxibustion(57.6%), meridian massage(44.2%), postpartum care(40.3%) and so on. 5) As for the degree of cognition on the establishment of health promotion center of oriental nursing by characteristics of subject, there was significant difference(F=4.03, p=.046) between male(3.01) and female(2.91). But there was no significant difference by age, marital status, level of educational achievement and monthly income. As the above result, cognition on the establishment of health promotion center of oriental nursing was relatively low because people were not familiar with about the health promotion center of oriental nursing yet. However once the center will be established, the degree of demand on the center will be relatively high. So positive advertisement will be necessary, and the management of useful programs will be also required in order to make people recognize the advantage when they actually will use the center. On the other hand, as the subject of the study consists of many young people of below 30, the health problem came to be low. And in the case of sampling, the study using random sampling that can represent population will be required.

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국제결혼 이주여성의 임신·출산 및 영유아 양육 지식과 교육요구도 (A Study on Pregnancy, Delivery, and Infant Rearing Knowledge and Educational Need of Marriage Immigrant Women)

  • 전미순;강기정;박성희
    • 농촌의학ㆍ지역보건
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    • 제36권3호
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    • pp.179-190
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    • 2011
  • 본 연구는 국제결혼 이주여성의 임신 출산 및 영유아 양육에 대한 지식과 교육요구도를 조사함으로써, 향후 국제결혼 이주여성을 위한 임신 출산 및 영유아 양육 교육프로그램 개발에 기초 자료로 활용하고자 시도되었다. 연구대상자는 충청남도 C시에 거주하는 국제결혼 이주여성으로 150명을 임의 표출방법으로 선정하였으나 응답이 불성실한 28부를 제외한 122명이었으며, 2010년 5월부터 8월까지 자료를 수집하였고, SPSS WIN 17.0 프로그램을 이용하여 분석하였다. 연구 결과, 대상자의 임신 출산 지식은 5점만점에 평균 3.35점으로 나타났고, 거주지역과 자녀유무에 따라 유의한 차이를 보였다. 임신 출산 교육요구도는 5점 만점에 평균 3.64점으로 나타났고, 출신국에 따라 유의한 차이를 보였다. 대상자의 영유아 양육 관련 지식은 5점 만점에 평균 3.16점으로 나타났고, 자녀유무 따라 유의한 차이를 보였다. 영유아 양육 교육요구도는 5점만점에 평균 3.66점으로 나타났고, 출신국과 자녀 유무에 따라 유의한 차이를 보였다. 국제결혼 이주여성의 임신 출산(p=0.003) 및 영유아 양육(p=0.001) 지식수준과 교육요구도간에는 유의한 차이를 보였으며, 지식수준보다 교육요구도가 유의하게 높게 나타났다. 본 연구결과, 임신 출산 및 영유아 양육 지식보다 교육요구도가 유의하게 높았으며, 임신 출산 및 영유아 양육 교육요구도는 유사한 수준으로 나타났다. 본 연구는 국제결혼 이주여성의 자녀 존재 유무 및 자녀의 연령에 제한을 두지 않았으며 또한 일지역 국제결혼 이주여성을 대상으로 실시된 연구이므로 본 연구결과를 일반화시키기에 제한이 있다. 따라서 향후 국제결혼 이주여성의 자녀 존재 유무 및 자녀의 연령에 제한을 둘 뿐 아니라 다른 지역의 국제결혼 이주여성을 대상으로 반복연구가 시도될 필요가 있다. 또한 국제결혼 이주여성의 임신 출산 및 영유아 양육 지식수준과 교육요구도에 영향을 미치는 요인들에 대한 탐색연구와 임신 출산 및 영유아 양육 지식을 향상시키기 위한 교육 및 상담프로그램의 적용과 이에 대한 효과검증 연구가 이루어져야 할 것이다.

악성종양 환자의 통증 및 통증관리에 관한 연구 (The Study of Pain and Pain Management of Cancer Patients)

  • 윤귀옥;박형숙
    • 기본간호학회지
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    • 제3권2호
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    • pp.299-316
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    • 1996
  • This study is the descriptive survey to provide basic data for nursing intervention to pain management of cancer patients by finding more effective way to manage pain with recognize pain level and pain characteristics. To achieve the purpose of this study, the subjects of this study are 110 male or female gastro intestinal tract patients who are older than twenty, are hospitalized in Pusan University Hospital from 1995. 5. 28 to 1995. 9. 25 and have had medical treatment. The modified pain assessment of cancer patients of Cornne, H. Rosermary, M. was used as the tool of study with 16 questionaries. The pain score consists of sensory intensity score and distress score. The data was analyzed by the SPSS statistical program number, percentage, mean, standard deviation, t-test, One Way Anova and Duncan's Multiple Range Test were utilized for analysis. The results were summarized as follows : 1. In population-sociological characteristics : in the age-range of subject, the sixties are most as 32.7% and the subjects after the forties are 89.5%, in sex of subjects, male patients are 66.4% and female 33.6%, in the number of family, the subjects who has 4 or above families are 70% and the subjects who live with their spouse, sons and daughters are 54.5% 2. In the disease characteristics : stomach cancer patients were most as 39.1%. And the most of patient who had never been operated before. In time of pain, the most of subjects were intermittent. In the type of pain, the most of subjects were 'dully pain' as 31.8%. Metastatic subjects were 30.0%. In the origin of pain, nervous pressure was 50.8%. The number of complication was 46 and most of complication are obstruction as 6%. 3. In the pain level, 91subjects complained pain. And mean pain score was $287.1{\pm}116.1$ The mean pain score of female subjects was higher than that of male subjects. 4. In the pain characteristics, the pain began usually at meal time as 40.7%. The duration of pain was mostly from 1 month to 3 months as 57.1%. The appetite was mainly concerned with the pain as 31.8%. The etiology of pain was usually tumor as 69.3%. The meaning of pain was incurable disease as 14.5%, anxiety, death and suffering. 5. The 56(61%) of 91subjects were treated with Analgesic pain management. The kinds of Analgesic is usually valentac as 46.4%. The medication was usually intramuscle as 66.1% at whenever necessary, Response of Analgesic after Medication was usually 'moderate release'. The side effects of medication were nausea as 26.8%. The average amount of morphine dosage hospitalized to cancer patients with pain was 80mg in a day and metastatic cancer patients with pain was 101.9mg in a day. 6. In the relation between the disease characteristics and pain level, there is a significant statistical difference : whether subjects had been operated or not : (t=2.88, p=0.005), time of pain is(t=3.34, p=0.005), stage of metastatic(F=9.323, P=0.0002), and type of pain(F=4.013, p=0.0008). In the pain level of diagnosis, Colon cancer was $353.3{\pm}81.7$(F=2.34, p=0.049), the origin of pain, nerve pressure $316.3{\pm}98.5$(F=2.44, P=0.045), In the complication, ascites and obstruction $324.9{\pm}96.8$(T=2.60, P=0.04).

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GPCR 냉동보관 세포의 활용을 위한 냉동조건의 최적화 연구 (Optimization of the cryopreserved condition for utilization of GPCR frozen cells)

  • 노효진;이승호
    • 한국산학기술학회논문지
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    • 제16권2호
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    • pp.1200-1206
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    • 2015
  • 신약 개발의 주요 표적이 되는 G-protein coupled receptor (GPCR)은 대부분의 생리적 활동에 관여하며 다양한 질병과 질환들에 관련되어 있다. GPCR을 타겟으로 하는 의약개발 연구에서 필수적인 실험방법으로 많이 활용되고 있는 세포기반 스크리닝 기술들은 사용되는 세포의 상태에 따라 데이터의 질이 좌우되는데 최근, 실험에 사용할 세포를 매번 배양하면서 소모되는 비용과 데이터의 변동을 줄이기 위해 냉동보관 세포를 적용하는 추세이다. 이에 본 연구에서는 단일 세포를 많은 양으로 배양하고 냉동 보관한 다음 사용되는 세포의 반응을 최적화하기 위하여 칼슘 검출을 위한 광 단백질이 포함된 세포주에 calcium sensing receptor와 urotensin II receptor가 안정적으로 발현되는 안정화 세포를 제작하고 $-80^{\circ}C$에서 보관한 다음 7 일 간격으로 실험했을 때 효능제와 길항제 반응을 비교하였다. 실험결과 보관기간이 증가함에 따라 세포 신호 값이 감소하였지만 $EC_{50}$$IC_{50}$ 값의 변화는 나타나지 않았다($EC_{50}:3.46{\pm}1.36mM$, $IC_{50}:0.49{\pm}0.15{\mu}M$). 그러나 액체질소에서 보관한 세포의 경우에서는 비냉동 세포와 비교하여 세포 신호 값이 감소했지만 보존기간에 따른 변화가 나타나지 않았으며 기간에 따른 $IC_{50}:0.49{\pm}0.15{\mu}M$$IC_{50}$의 변화도 없었다. 보관기간이 경과 될수록 세포의 신호 값이 감소하는 것은 세포 손상도 증가가 원인인 것으로 판단되며, 이러한 결과들로부터 장기간 냉동 보관을 위해서는 액체질소를 이용하는 것이 가장 효과적이고 한 달 이내 단기간 사용의 목적으로는 $-80^{\circ}C$ 보관조건도 가능할 것으로 판단된다. 이와 같이 냉동세포의 적극적인 활용을 통하여 초기 스크리닝 과정에서 나타나는 실험 유동성을 감소시킬 수 있을 것으로 예상된다.

한국 노인의 사회활동이 건강수명에 미치는 영향에 대한 생존분석: 성별 비교를 중심으로 한 13년간 분석 (Impact of Social Activities on Healthy Life Expectancy in Korean Older Adults: 13-Year Survival Analysis Focusing on Gender Comparison)

  • 양승민;최재성
    • 한국노년학
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    • 제41권4호
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    • pp.547-566
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    • 2021
  • 본 연구의 목적은 노인의 성별에 따른 사회활동 유형별 참여가 건강수명에 미치는 영향력을 분석하는 것이다. 이를 위해 고령화연구패널조사(KLoSA)의 1차(2006년)에서 7차(2018년)까지의 13년간 데이터 중 65세 이상 노인 4,029명(남성노인=1,710명, 여성노인=2,319명)의 자료를 사용하여 생존분석의 하나인 Cox 비례해저드모형(Cox proportional hazard model)으로 분석을 시도했다. 분석결과 첫째, 여성노인의 건강수명은 75.34세, 남성노인의 건강수명은 76.94세로 나타났다. 여성노인은 남성노인에 비하여 질병 및 장애의 발생 강도와 발생 비율이 더 컸고, 질병 및 장애가 발생한 노인의 비율도 더 높았다. 둘째, 남성노인과 여성노인의 건강수명에 공통적인 영향요인은 연령, 만성질환 수와 주관적 건강상태로 나타났다. 학력은 남성노인의 건강수명에만 유의한 것으로 나타났고, 거주지역, 과거 음주 경험, 삶의 만족도는 여성노인의 건강수명에만 유의한 것으로 나타났다. 셋째, 건강수명에 유의한 영향을 미치는 사회활동 유형은 남성노인의 경우 종교활동과 연고집단활동, 여성노인의 경우 종교활동으로 나타났다. 본 연구는 노인의 건강수명에 대한 사회적 활동 유형의 효과가 성별에 따라 다르다는 점을 보여준다. 또한, 건강수명 단축의 위험은 남성노인보다 여성노인이 상대적으로 높게 나타났다. 연구자들은 건강수명 연장을 위하여 성별 특성을 고려한 사회활동 참여의 기회 확대와 유형별 참여의 행태나 질에 대한 고려, 그리고 남녀 간 건강격차를 감소시키기 위한 정책개발의 필요성을 제안하였다.