• Bulletin of the Korean Chemical Society
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• 제33권11호
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• pp.3727-3734
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• 2012

An analytical method has been developed for the rapid determination of perfluorinated compounds (PFCs) in human serum samples. The extraction and purification of PFCs from human serum were performed by the modified method of previous report. Ten PFCs were rapidly separated within 3.3 min by C18-monolithic column liquid chromatography (LC) and detected by electrospray ionization (ESI) tandem mass spectrometry (MS/MS) in negative ion mode. The runtime of PFCs on monolithic column LC was up to 4-fold faster than that on conventional column LC. The effect of triethylamine (TEA) to the mobile phase has investigated on the overall MS detection sensitivity of PFCs in ESI ionization. Quantification was performed by LC-MS/MS in multiple-ion reaction monitoring (MRM) mode, using $^{13}C$-labeled internal standards. Method validation was performed to determine recovery, linearity, precision, and limits of quantification, followed by, the analysis of a standard reference material (SRM 1957 from NIST). The overall recoveries ranged between 81.5 and 106.3% with RSDs of 3.4 to 16.2% for the entire procedure. The calibration range extended from 0.33 to 50 $ng\;mL^{-1}$, with a correlation coefficient ($R^2$) greater than 0.995 and the limits of quantification with 0.08 to 0.46 $ng\;mL^{-1}$. This approach can be used for rapid and sensitive quantitative analysis of 10 PFCs in human serum with high performance and accuracy.

• 한국안전학회지
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• 제36권2호
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• pp.87-93
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• 2021

Modern society shows rapid growth that is different from that of the development of existing technologies. The development of these technologies has led to the tendency of buildings to become dense, large and advancing. Regarding fire hazards, the possibility of large-scale fires causing fatal damage, due to the rapid spread of fire, increases. Therefore, for this reason, fire defense, i.e. detection and fire extinguishing facilities, in buildings are essential and well applied. But there are always limitations to that. Based on this reason, we would like to suggest the introduction of a new concept of a fire safety system. The method presented here is not only to use a single system for fire detection and fire extinguishing systems but to jointly use it in the environment and energy management fields within the building. However, an important step is required before introducing a system of these technologies. The fire extinguishing method proposed by this system is a method of extinguishing by blocking oxygen flowing into the space where the fire occurred. However, a sufficient basis is needed for this system to be applied in practice. Therefore, in this study, we intend to conduct a preliminary experiment to introduce the new concept of fire detection and extinguishing. The experiment used ethanol with a relatively simple combustion reaction and a high possibility of complete combustion. As a result, it was confirmed how the internal values changed during a fire using ethanol. Resultingly, we obtained the internal oxygen concentration and internal environmental changes according to the initial flame size. Lastly, the data accumulated in this study can be used as data for application in an automatic fire extinguishing system.

• 한국식품과학회지
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• 제39권5호
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• pp.488-493
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• 2007

국내유통중인 곡류, 견과류 및 그 가공품 총 25품목, 393건의 시료에 대해 immunoaffinity column 정제방법을 이용하여 총아플라톡신 오염실태를 조사하였으며, 그 결과 곡류 및 곡류가공품 6건, 견과류 및 견과류 가공품 37건에서 아플라톡신 오염이 확인되었으며 오염수준은 아플라톡신 $B_1$으로서 $0.04-2.65{\mu}g/kg$, 총아플라톡신으로서 $0.04-5.51{\mu}g/kg$ 범위로 나타났다. Immunoaffinity column 정제를 거쳐 HPLC-FLD로 분석한 결과 아플라톡신이 검출된 시료에 대해서 LC-MS/MS로 확인하였으며, 그 결과 모두 아플라톡신으로 확인되었다. 본 연구결과에서 나타난 곡류 및 견과류에 대한 아플라톡신 검출빈도 및 오염수준은 국내, 외 연구 결과와 유사하거나 비교적 낮게 나타났으며 국내 아플라톡신 기준 및 미국, CODEX에서 설정된 기준규격 이하로 검출되었다.

• Food Science and Biotechnology
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• 제17권2호
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• pp.408-412
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• 2008

Determining the elution of water-soluble substances from herbal teas is an important factor in their efficient use in terms of taste, perfume, and content of health-related components. The antioxidant activity and content of catechins in commercial Chrysanthemum indicum (gamguk) teas were determined for optimum elution conditions. The water extract of gamguk teas did not differ significantly in yield compared to methanol extracts and showed stronger antioxidant activity. Catechin contents in gamguk teas were 8-18% of the extracts when individual peaks in high-performance liquid chromatography analysis were compared to standard catechin peaks. Gamguk teas exhibited faster release of antioxidants, and the antioxidant activity was positively correlated with the thermal treatments. Gukhwacha (GC) was the best tea for rapid release (30 sec) of antioxidants with the $50^{\circ}C$ treatment, whereas antioxidants in other teas were relatively slower released.

• Mass Spectrometry Letters
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• 제2권2호
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• pp.37-40
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• 2011

Saccharin is a commonly used artificial sweetener in foodstuffs. However, for its carcinogenic dispute, it has been regulated by government bodies. In this study, isotope dilution mass spectrometry (ID-MS) was introduced for the accurate quantification of saccharin. To employ ID-LC/MS, we obtained its isotope analogue, $^{13}C_1$-sodium saccharin, by customized synthesis. Samples were spiked with $^{13}C_1$-sodium saccharin and analyzed with LC/MS in negative mode. Chromatographic conditions were optimized for the adequate chromatographic retention and separation of saccharin with a $C_{18}$ column. MS was operated with electrospray ionization by the selected ion monitoring (SIM) mode of $[M-H]^-$ for saccharin (m/z 182) and $[M-Na]^-$ for its isotope analogue (m/z 183). To validate the ID-LC/MS method for accurate measurement, we prepared a batch of a candidate material by sortifying quasi-tea-drinks with saccharin and analyzed samples gravimetrically fortified in various levels of concentration. The repeatability and reproducibility of this method was tested by analyzing the reference material. Result show that ID-LC/MS is a reliable method for the quantitative analysis of saccharin.

• 한국약용작물학회지
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• 제24권5호
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• pp.351-359
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• 2016

Background: We investigated the optimal aqueous extraction conditions for recovery of high yields of total phenolic compounds from roots of Arctium lappa L. (burdock, Asteraceae), and we compared their antioxidant capacity. Methods and Results: The antioxidant activity of the extracts was tested using 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)-diammonium salt, and oxygen radical absorbance capacity assays. In addition, the major phenolic compounds present in the extracts were determined by high performance liquid chromatography analysis. Our results suggest that the roasted burdock $100^{\circ}C$, 15 min extract exhibited the strongest radical scavenging activity and possessed the highest concentration of phenolic compounds. The polyphenol content of both dried burdock and roasted burdock significantly increased with increase in the extraction temperature and time. Conclusions: These results indicated a relationship between phenolic compound levels in burdock and their free radical scavenging activities. This suggests that phenolic compounds significantly increase the antioxidant potential of burdock extracts.

• 한국의사학회지
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• 제33권2호
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• pp.129-145
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• 2020

This study studied the use of Woohwanggo in the Joseon royal family through The daily records of Royal secretariat of Joseon Dynasty, which contains detailed records of royal medical treatment. The study found that Woohwanggo was mainly used for diseases related to vexing heat (煩熱), fumigating heat (薰熱), night fever (夜間發熱), and heat syndrome (熱證) from smallpox, and measles in the royal family of Joseon. This study also confirmed that Woohwango was used in various ways within the Daily records of Royal secretariat of Joseon Dynasty, including the way it was taken with various types of tea, the way it was made into liquid form, and the way it was used as an external agent for skin diseases. Further findings were compared to the medical books, and the dosage characteristics of Woohwango in the daily records of Royal secretariat of Joseon Dynasty were discussed. and the medical perception of King Yeongjo (英祖), which was examined during the study, was also discussed.

• 대한영양사협회학술지
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• 제10권1호
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• pp.58-64
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• 2004

This study was performed to investigate the kinds of health food advertised and sold through media like printed matter, TV broadcasting or internet. It was found that fifty three percent of the health food was circulated through internet, 27% through printed matter, 9% through TV Broadcasting. When the health foods was classified, the proportion of special purpose nutritious foods was 36.1%, health aid foods 33.9%, uncooked powdered foods 18.5%, functional foods for health 4.9%, Ginseng products 3.9%, and tea 0.9%. The special purpose nutritious foods was composed of 59% of nutrition supplementary foods, 24% of dietary fiber foods, 9% of weaning foods, 7% of milk formula and 2% of HCA. Vegetable extracts occupied 21.1% of health aid foods, yeast 7.5%, mushroom extracts 7.0%, chitosan 6.2%, aloe 5.3%. Dried powdered type health food occupied the highest proportion. The other type were capsule(18.8%), tablet(18.1%), and liquid type(16.4%). When the health food was classified with health claim, the proportion of "nutrition supplementation" was the highest (23.9%), "diet" 14.9%, "ergogenic"(18.8%), "promoting bowel movement" 7.8%, "growth" 5.7%, "regulation of blood sugar" 4.5%, "improving of immunity"(2.4%) and anti-aging effect(2.4%).

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 1995년도 제5회 학술강연회논문집
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• pp.49-55
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• 1995

이원 액체추진제를 사용하는 인공위성용 로켓 추진기관의 개발을 위한 핵심부품별 개념 및 상세설계, 성능해석, 성능실험용 시작품의 제작, 수류 성능실험 및 지상 연소시험이 수행되었다. 인공위성 궤도조종용 로켓 추진기관은 1.38MPa의 연소실 압력으로 4초동안 1780N(400$Ib_f$)의 평균추력을 내도록 설계되었으며, 산화제로는 질산, 연료로는 트리 에틸렌 아민(triethylene amine, TEA)과 자이리딘(xylidine)의 혼합물로 구성된 접촉발화형 이원 액체추진제를 사용하고, 추진제를 가압방식에 의해 연소실에 분사하는 방법으로 분사충돌, 미립화, 그리고 기화 후 연소시키게 된다. 효율적인 설계를 위하여 설계전용 소프트웨어를 개발하였으며, 추진기관의 핵심부품별로 유동 시뮬레이션을 수행하고, 해석결과와 수류 실험결과를 바탕으로 설계를 수정, 보완하였다. 지상 연소시험 및 수류 성능실험을 위하여 추진제 공급장치 및 계측 시스템이 설계, 제작되었고, 시스템의 작동 및 자료처리를 위한 소프트웨어를 개발하여 수류 성능실험 및 지상 연소시험에 사용하였으며, 연소시험결과 지상 평균추력 378$Ib_f$를 발생하였다.

• Tribology and Lubricants
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• 제38권6호
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• pp.241-246
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• 2022

This paper presents the effects of cryogenic temperature on the wear behavior of 22MnB5 blank under cold stamping. After immersing the blank in liquid nitrogen (LN2) for 10 min, a strip drawing test was performed within 10 s. The hardness was measured using the Rockwell hardness test, which increased from 165 HV at 20℃ to 192 HV at cryogenic temperature. The strip drawing test with 22MnB5 blank and SKD61 tool steel shows that for the different wear mechanisms on the tool surface with respect to temperature; adhesive wear is dominant at 20℃, but abrasive wear is the main mechanism at cryogenic temperature. As the friction test is repeated, sticking gradually increases on the tool surface at 20℃, whereas the scratch increases at cryogenic temperature. For the friction behavior, the friction coefficient rapidly increases when adhesive wear occurs, and it occurs more frequently at 20℃. The results for nanoindentation near the worn blank surface indicate a difference of 1.3 GPa at 20℃ and 0.8 GPa at cryogenic temperature compared to the existing hardness, indicating increased deformation by friction at 20℃. This occurs because thermally activated energy available to move the dislocation decreases with decreasing temperature.