• Journal of Food Hygiene and Safety
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• v.29 no.4
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• pp.292-298
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• 2014

Tridemorph is a systemic morpholine fungicide for crops. The objective of this study was to develop reliable and sensitive analytical method for determination of tridemorph residues in tea samples for ensuring the food safety. Tridemorph residues in samples were extracted with acetonitrile after hydration, partitioned with saline water, and then purified using an aminopropyl ($NH_2$) SPE cartridge. The purified samples were detected and quantified using LC-ESI-MS/MS. The linear detection limits for tridemorph ranged from 0.02 to $1.0mgL^{-1}$ with a correlation coefficient of 0.9999. The method was validated using tea samples spiked with tridemorph at different concentration levels (0.02 and $0.05{\mu}gmL^{-1}$). The average recovery ranged between 75.0 and 84.7% with relative standard deviations less than 10%. The LOD and LOQ were 0.01 and $0.02mgL^{-1}$, respectively. The developed method was applied successfully to the identification of tridemorph in real tea samples obtained from different sources, and tridemorph was not detected in any of the samples. The results show that the developed analytical method is accurate and suitable for tridemorph determination in tea samples.

• Mass Spectrometry Letters
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• v.2 no.4
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• pp.92-95
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• 2011

Pyrophosphates are the key intermediates in the biosynthesis of isoprenoids, and their concentrations could reveal the benefits of statins in cardiovascular diseases. Quantitative analysis of five pyrophosphates, including isopentenyl pyrophosphate (IPP), dimethylallyl pyrophosphate (DMAPP), geranyl pyrophosphate (GPP), farnesyl pyrophosphate (FPP), and geranylgeranyl pyrophosphate (GGPP), was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in negative ionization mode. After dilution with methanol, samples were separated on a 3 ${\mu}m$ particle multi-modal $C_{18}$ column ($50{\times}2$ mm) and quantified within 10 min. The gradient elution consists of 10 mM ammonium bicarbonate and 0.5% triethylamine (TEA) in water and 0.1% TEA in 80% acetonitrile was used at the flow rate of 0.4 mL/min. Overall recoveries were 51.4-106.6%, while the limit of quantification was 0.05 ${\mu}g$/mL for GPP and FPP and 0.1 ${\mu}g$/mL for IPP, DMAPP, and GGPP. The precision (% CV) and accuracy (% bias) of the assay were 1.9-12.3% and 89.6-111.8%, respectively, in 0.05-10 ${\mu}g$/mL calibration ranges ($R^2$ > 0.993). The devised LC-MS/MS technique with the multi-modal $C_{18}$ column can be used to estimate the biological activity of pyrophosphates in plasma and may be applicable to cardiovascular events with cholesterol metabolism as well as the drug efficacy of statins.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.4
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• pp.636-641
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• 2002

Optimum extraction condition and clarification process were investigated to manufacture liquid extract tea using Phellinus linteus. Carbohydrates content in the raw Phellinus linteus was 80.9%. Major minerals were K, Ca, Fe and Na, but vitamin B$_1$, B$_2$, and C were not detected. The best extraction condition was 5% raw material at 10$0^{\circ}C$ within 3 hr. To clarify the extract, three methods of filtration with depth filter pad, centrifugation and addition of several filter aids were studied. Filtration with depth filter pad and centrifugation were highly effective on the clarification and the changes in the turbidity for 7 weeks at 4$0^{\circ}C$ were not occurred. The optimum clarification condition was centrifugation above 6,000 rpm or filtration with 8 ${\mu}{\textrm}{m}$ depth filter pad.

• Applied Chemistry for Engineering
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• v.1 no.1
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• pp.63-72
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• 1990

Surface of crystalline silica was sequentially reacted with silane(A 187), liquid rubber(CTBNx8) and vinyl monomer(AA, MMA, 2-HEA, GMA) in existance of amines(TEA, CTMAB, BETAC) or peroxide(BPO). By mixing it with epoxy resin at a ratio 0~36%(volume %) of total component, liquid properties of mixtures was investigated experimentally. i) Coating ratio depended on quantity and sorts of catalyst. ii) Total coating of 2.5~5.8% was attained by using 0.1~2.0% of catalyst. iii) Treated surfaces represented each different features in according to sorts of treatment. iv) Silane/rubber or silane/rubber/vinyl represented lower viscosity and settling than non-treated or silane-treated.

• Proceedings of the KSME Conference
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• 2001.06d
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• pp.846-851
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• 2001

For the successful development of the main engine of KSR(Korea Sounding Rocket)-III, Korea Aerospace Research Institute(KARI) carried out the experimental study on the subscale model engines. Several types of engines were tested on the Small Liquid Rocket Engine Test Facility. One of the typical test results of a Sub. engine(Sub. Mod.3) is presented here. It uses the Jet A-1 as fuel, liquid oxygen as oxidizer, and Tri-Ethyl Aluminium(TEA1) as ignition agent. The gas pressure feed system is adopted as a feeding mechanism and the design chamber pressure is 200psia. The physical phenomena are described in three regimes(ignition, transient, and steady state) with the pressure, thrust, flowrate and image data. And the pressure oscillation is analyzed in Fourier domain (<500Hz). Then we conclude that in this experiment, the engine shows the characteristic low frequency of 80Hz and it is stable for that frequency of pressure oscillation.

• 보존과학연구
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• s.15
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• pp.21-32
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• 1994

Optimum conditions for benzene synthesis and liquid scintillation counting have been studied for the determination of radiocarbon age. In benzene synthesis the carbon dioxide converted to benzene with high efficiency of 91%. Yields of each step with 10L of carbon dioxide were $CO_2$ $\rightarrow$$C_2H_2$(94%), $C_2H_2$$\rightarrow$$C_6H_6$(96%) and$CO_2$$\rightarrow$$C_6H_6$(91%), respectively. Benzene synthesized from oxalate was measured with purity of 95% by GC /MS. $\delta^13$(C$^13$C/$^12$C) of oxalate was measured to $-24.7\{textperthousand}$ by massspectrometer. For liquid scintillation counting of benzene sample low background and highest FOM were measured in 0.5 ml cocktail and 3 ml standard solution with the range of 15.4∼74.9 KeV window setting. Oxalate and background samples weremeasured to $28.7\pm0.12$cpm and $3.92\pm0.04$ cpm in 15.4∼74.9 KeV

• Korean Journal of Food Science and Technology
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• v.38 no.1
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• pp.5-9
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• 2006

Method for separation and quantification of methylxanthins, catechins, and theaflavins in Korean commercial teas (green, oolong, and black teas) was developed using reversed phase high-performance liquid chromatography (HPLC). After extraction with hot water, tea compounds were separated on Inertsil ODS-3v $(5\;{\mu}m)$ column, eluted with gradient of 7% acetonitrile and 93% of 20 mM phosphate buffer mixture for 7 min. Column effluent was monitored at 270 nm. This technique was effective for analyses of m methylxanthins, catechins, and theaflavins in teas and biological samples. In green and oolong teas, two kinds of methylxanthins and 7 of catechins were identified, whereas 4 theaflavins were only identified in black tea. Among seven catechins in green and oolong teas, EGCG showed highest amount, whereas ECG was highest in black tea. (theaflavins were found only in black teas) In all teas, theobromine content was lower than that of caffeine.

• Korean Journal of Agricultural Science
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• v.47 no.4
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• pp.1071-1081
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• 2020

Essential oils (EOs) have been shown to be plant-extracted antimicrobial agents. However, there are limited studies investigating the efficacy of EOs against pathogens. Among them, tea tree oil (TTO) is extracted from Melaleuca alternifolia, which is also used as an antifungal agent. In this study, the effect of TTO was investigated on the suppression of melon powdery mildew caused by Podosphaera xanthii and tomato leaf mold disease caused by Passalora fulva. Both powdery mildew and leaf mold diseases were significantly suppressed by a spray of TTO. Eighty percent of powdery mildew and 81% of leaf mold disease of the control value were suppressed by 0.5% TTO liquid, when sprayed 3 times every 7 days on the melon and tomato leaves. Inhibition of mycelial growth was also greatly affected by different concentrations of TTO against four different fungal pathogens. Ninety-eight percent of Pseudocercospora fuligena, 97% of P. fulva, 95% of Botrytis cinerea, and 94% of Phytophthora infestans mycelial growth were inhibited by 0.2% to 1.0% of TTO contained in plate media, respectively. However, phytotoxicity in plants by the TTO treatments was revealed when melon and tomato leaves were sprayed with a 1% and 2% concentration of TTO, respectively. Therefore, our findings show that TTO has high antifungal effects against various plant pathogens that occur during crop cultivation. We also suggest that when applying TTO to plant leaves, it is necessary to establish an accurate treatment concentration for different crops.

• Korean Journal of Food Science and Technology
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• v.47 no.5
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• pp.556-560
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• 2015

This study was aimed at investigating the natural occurrence of caffeine, theobromine, and theophylline in tea leaves. Sample clean-up was based on a simple solid phase extraction (SPE) treatment, and simultaneous analysis was performed by high performance liquid chromatography (HPLC). This method showed good results in terms of linearity, recovery, precision, limit of detection (LOD), and limit of quantitation (LOQ). The caffeine, theobromine, and theophylline contents of tea leaves (n = 228) ranged from 5.4 to 58.2 mg/g, from not detected to 11.2 mg/g, and from not detected to 0.7 mg/g, respectively. The results obtained in this study can be used as fundamental data of caffeine, theobromine, and theophylline contents in tea leaves.

• Analytical Science and Technology
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• v.25 no.1
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• pp.25-32
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• 2012

Isotope dilution mass spectrometry (ID-MS) based on liquid chromatography tandem mass spectrometry (LC/MS/MS) was developed for the accurate determination of sorbic acid in tea-drink. An isotope analogue of sorbic acid, $^{13}C_2$-sorbic acid, was obtained by custom synthesis. MS was operated in the negative mode with selected reaction monitoring (SRM) mode of $[M-H]^-$ ${\rightarrow}$ $[M-CO_2H]^-$ channel at m/z 111 ${\rightarrow}$ 67 for sorbic acid and at m/z 113 ${\rightarrow}$ 68 for its isotope analogue. Chromatographic separation was accomplished with a C18 column and an isocratic mobile phase of 55% of 50 mM ammonium acetate (pH 4.5) and 45% of methanol. Homogeneous reference materials were prepared for validation of this method, including repeatability and reproducibility tests, by fortifying tea-drink with sorbic acid in our laboratory. Repeatability and reproducibility studies showed that the ID-LC/MS method is a reliable and reproducible method which provides less than 3.8% of relative standard deviation (RSD) for the analysis of sorbic acid.