• 한국가축번식학회지
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• 제21권1호
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• pp.19-23
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• 1997

This present study was carried out to examine the effect of maturation media and liquid boar semen on in vitro maturation and feritilization of pig oocytes. The results obtained were as follows : When the oocytes were cultured for 36∼42 hours in mTCM-199, Waymouth MB 725/1 and mTLP-PVA medium, the maturation rates were 90%, 92% and 88%, respectively. The sperm penetration rates of pig oocyte matured in vitro were 87%(mTCM-199), 90%(Waymouth MB 725/1) and 86%(mTLP-PVA), respectively. The rates of nuclear maturation and fertilization of pig oocytes among three different media did not differ. However, the rate of male pronucleus formation of pig oocytes was significantly higher in pig oocytes matured in Waymouth MB 725/1(91%) than oocytes matured in mTCM-199(66%) and mTLP-PVA(62%) medium (P<0.05). When the collected sperm-rich fraction without diluent was used fro in vitro fertilization in mTCM-199 fertilization medium, the fertilization rate was 87.9%. However, when the liquid boar semen diluted with B tschwiler diluent was used at day 3 and 5 after dilution, the fertilization rate was 40.8% and 0.0%, respectively.

• 한국산림과학회지
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• 제111권4호
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• pp.594-602
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• 2022

본 연구는 편백의 초기생장에 대한 slurry composting and biofiltration(SCB)액비의 효과를 분석하기 위해 대조구(control), 화학비료처리구(chemical fertilizer : CF), 퇴비처리구(compost : CP), 저농도액비처리구(low liquid fertilizer : LLF) 및 고농도액비처리구(high liquid fertilizer : HLF)로 조성하여 처리구별 5본씩 3반복 실험을 하였다. 생장량분석 결과, 고동노액비를 200% 처리한 HLF-200처리구에서 가장 높은 생장량을 보였다. 시비효과를 분석하기 위해 HLF-200처리구와 대조구를 대상으로 근원직경 및 수고 생장모델을 개발하였다. 그 결과, 대조구와 HLF-200처리구 근원직경생장모델의 경우 각각 Schumacher 동형방정식 및 Schumacher 다형방정식이 가장 적합한 것으로 나타났으며, 수고생장모델의 경우 Gompertz 다형방정식이 가장 적합한 것으로 나타났다. 결정된 모델에 따른 생장곡선을 통해 근원직경과 수고의 생장량 및 생장률 모두 대조구보다 HLF-200처리구가 높아 시비효과가 있는 것으로 나타났으며, SCB액비를 통한 시비처리가 편백의 초기생장 증진에 적합한 것으로 판단되었다.

• 한국축산시설환경학회지
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• 제18권sup호
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• pp.7-12
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• 2012

To prevent the inappropriate treatment of livestock manure and induce the correct circulation of them into farmland, systematic monitoring and management by the treatment and circulation stage of livestock manure are required. The purpose of this study was to apply ubiquitous information communictation technology (u-ICT) to a livestock waste management system for better treatment by utilizing ubiquitous computing technology in the livestock sector. Elements and levels of applicable u-ICT technology for efficient livestock manure management were derived by analyzing previous researches. In addition, a conceptual diagram of an integrated management system was suggested by analyzing the existing liquid fertilization process.

• 한국유기농업학회지
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• 제26권4호
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• pp.731-743
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• 2018

배추 유기재배 시 녹비작물과 추비방법이 배추의 생육과 질소이용에 미치는 영향을 구명하기 위하여 2012년부터 2014년까지 수원에 있는 국립농업과학원 포장에서 시험을 수행하였다. 녹비작물은 여름철 크로타라리아와 겨울철 헤어리베치를 이용하였다. 녹비처리 방법은 전면경운 토양혼입방법과 부분경운 토양혼입방법, 그리고 무경운 예취피복처리를 비교하였다. 추비방법은 녹비만 처리한 것과 녹비 처리 후 유박과 액비로 추비한 처리를 비교 하였다. 시험결과 녹비 처리 방법은 예취 후에 경운하여 토양혼입 하는 것보다 무경운 피복처리 하는 것이 좋았다. 봄배추재배 시 동계 녹비작물 헤어리베치 재배로 정상적인 생육과 수량이 가능하였고 관행의 화학비료 처리구에 비하여 무름병과 Tip-burn 발생이 적어 수량성도 높았다. 가을재배에서는 녹비 연속재배와 유박 추비로 관행의 화학비료처리구와 비슷한 수량을 보였다. 하지만 하계 단기성 녹비작물 재배 시에는 관행 수량의 70% 내외에 그쳤다. 녹비재배에 이은 액비 활용 양분관리로 관행과 비슷한 수량이 가능하였고 토양중 무기태 질소가 안정적으로 유지되고 질소이용효율도 향상되었다. 결론적으로 배추 유기재배를 위해서는 겨울과 여름 녹비작물을 재배하고 가을 작기를 선택하여 액비로 추비하는 것이 권장된다.

• 한국토양비료학회지
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• 제46권3호
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• pp.173-181
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• 2013

Livestock wastes are considered as major environmental pollutants because they contain high concentration of organic materials. In 2001, The Environmental Department reported that stock farmers were increasing as 5.1%/year, which resulted in a gradual increase in livestock wastes generation. The direct disposal of livestock wastes create several environmental problems. Thus, several countries banned the disposal of livestock wastes in environment including aquatic systems. Recently, aeration-based liquid fertilization was considered as potential way for the disposal of livestock wastes. In this study, next generation sequencing (NGS) analysis was used to understand the microbial community changes during liquid fertilization of livestock wastes. Microbial community was compared with liquid fertilizer physicochemical analysis such as $BOD_5$, $COD_{Mn}$ pH, N (Nitrogen), P (Phosphorus), K (Potassium) etc. The physicochemical parameters and bacterial community results pave the way for producing effective livestock-based fertilizer. By comparing the physical characteristics of the manure with microbial community changes, it is possible to optimize the conditions for producing effective fertilizer.

• 한국작물학회지
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• 제56권2호
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• pp.140-145
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• 2011

벼에 대한 SCB 액비의 활용방법을 구명하기 위하여 기 추 비 시용량을 달리 처리한 후 쌀 수량 및 토양환경에 미치는 영향 등을 검토한 결과는 다음과 같다. 논토양의 화학성은 규산을 제외한 액비시용구가 무시용구에 비하여 높았으나 시험 전 후 간에는 큰 차이가 없었다. 액비시용 후 토양 중 $NH_4$-N함량은 2일 이내 균평작업으로 논 전체에 균일한 농도를 유지하였다. 액비분시에 따른 토양 중 $NH_4$-N와 $NO_3$-N함량은 이앙 후부터는 초기에는 화학비료 관행시비구와 액비 기비 100%처리에서 높았으나 분얼감수기 이후에는 모든 처리가 유사하였다. 토양침출수중 $NO_3$-N함량은 액비기비70%+수비30%시용에서 관행시비구보다 약간 낮은 함량을 보였다. 액비 분시에 따른 쌀 수량은 관행시비구보다 액비 기비 100%처리에서 약간 낮았으나 액비 기비70%+수비30%에서 관행시비구와 대등하였으며 나머지 액비 처리구에서도 큰 차이가 없었다. 쌀 품위는 무 시용구와 액비 기비100%에서 완전립율이 높고, 단백질함량이 낮았으나 액비기비70%+수비30%에서 관행시비구와 유사하였다. 따라서 SCB액비를 이용하여 벼 재배할 경우 액비기비70%와 수비30%시용으로 화학비료 사용을 대체 할 수 있을 것으로 판단된다.

• 한국초지조사료학회지
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• 제20권1호
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• pp.55-60
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• 2000

This experiment was carried out to determine the effect of cutting frequency(3rd and 5th cut) and level of nitrogen fertilization(l50 + liquid manure, 300 and 450kgha) on growth characteristics, dry matter yield and nutritive vaule of reed canarygrass(Pha1aris arundinacea L.), at the forage experimental field, College of Agriculture and Life Sciences, SNU, Suweon. Plant height of 3rd cutting(79.2cm) was significantly higher than that of 5th cutting(58.0cm). And dry mattter yield of cutting frequency was observed in 3rd cutting(16,902kgPna) and 5th cutting(l7,833kg/ha) As the nitrogen fertilization was increased, DM yield was significantly increased(p<0.05). When the nitrogen fertilization was applied at the level 150 + liquid manure, 300 and 450kg, dry matter yields were 16,5 12, 17,123 and 18,368kg/ha, respectively. The contents of neutral detergent fiber(NDF), acid detergent fiber(ADF) and in vitro dry matter digestibility(1VDMD) were similar among cutting frequency. However, slightly higher NDF, ADF and IVDMD contents were found in 5th cutting, but there was not affected by nitrigen fertilization. Based on the results of this experiment, it were indicated that to 5th cutting and 450kg nitrogen fertilization could be obtained from the increase of dry matter yield and nutritive value (Key words : Cutting frequency, Nitrogen fertilization, Dry matter yield, Nutritive Value, Reed canarygrass)

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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• pp.63-63
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• 2003

This study was carried out to investigate the effects of liquid boar sperm stored at 4$^{\circ}C$ on sperm motility, normal acrosome, and in-vitro fertilization and culture of pig oocytes matured in-vitro. The sperm-rich fraction (30~60 ml) of ejaculate was collected into an insulated vacuum bottle. Semen was slowly cooled to room temperature (20~23$^{\circ}C$) by 2 h after collection. Semen was transferred into 15 ml tubes, centrifuged at room temperature for 10 min at 800$\times$g, and the supernatant solution was poured off. The concentrated sperm was resuspended with 5 ml of lactose, egg yolk and N-acetyl-D-glucosamine (LEN) diluent to provide 1.0$\times$10$^{9}$ sperm/ml at room temperature. The resuspended semen was cooled in a refrigerator to 4$^{\circ}C$ and preserved for 5 days to examine sperm motility and normal acrosome. The medium used for oocyte maturation was modified tissue culture medium (TCM) 199. After about 22 h of culture, oocytes were cultured without cysteamine and hormones for 22 h at 38.5$^{\circ}C$, 5% $CO_2$ in air. Oocytes were inseminated with liquid boar sperm stored at 4$^{\circ}C$ for 2 days after collection. Oocytes were coincubated for 6 h in 500 ${mu}ell$ mTBM fertilization media with 0.2, 1, 5 and 10$\times$10$^{6}$ /ml sperm concentration, respectively. At 6 h after IVF, oocytes were transferred into 500 ${mu}ell$ Hepes-buffered NCSU-23 culture medium for further culture of 6, 48 and 144 h. There were significant differences in sperm motility and normal acrosome among preservation days and incubation times, respectively. The rates of sperm penetration and polyspermy were higher in 5 and 10$\times$10$^{6}$ sperm/ml than in 0.2 and 1$\times$10$^{6}$ sperm/ml. Male pronuclear formation was lower in 0.2$\times$10$^{6}$ sperm/ml than in 1, 5 and 10$\times$10$^{6}$ sperm/ml. Mean numbers of sperm in penetrated oocyte were highest in 10$\times$10$^{6}$ sperm/ml compared with other sperm concentrations. The rate of blastocysts from the cleaved oocytes (2~4 cell stage) was highest in 1$\times$10$^{6}$ sperm/ml compared with other sperm concentrations. In conclusion, we found out that liquid boar sperm stored at 4$^{\circ}C$ could be used for in-vitro fertilization of pig oocytes matured in-vitro. Also, we recommend 1$\times$10$^{6}$ ml sperm concentration for in-vitro fertilization of pig oocytes.

• 한국축산시설환경학회지
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• 제19권1호
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• pp.47-54
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• 2013

Two types of reactors were set to investigate the change of characteristics of pig slurry by aeration during fertilization period. One system was equipped with air diffuser to supply oxygen to pig slurry for liquid fertilization, but there was no air diffuser in the other system. Air supply to the experimental systems was regulated by air flow meter. The reactors were set up in the laboratory to protect the pig slurry from external condition such as temperature and humidity changes. Maintaining optimal pH range in the experimental reactors is an important factor for liquid fertilization of pig slurry. In this study, pH ranges of aerobic reactor and anoxic reactor was 7.04~7.19 and 7.34~7.81, respectively. The temperature of aerobic reactors was $2{\sim}3^{\circ}C$ higher then indoor temperature. The amount of sludge accumulated at the bottom layer of non-aerated reactors was 4~5 times more than that of aerated reactors.

• Asian-Australasian Journal of Animal Sciences
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• 제17권10호
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• pp.1369-1373
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• 2004

The percentages of sperm motility and normal acrosome on the liquid boar semen diluted and preserved at $4^{\circ}C$ with lactose hydrate, egg yolk and N-acetyl-D-glucosamine (LEN) diluent were significant differences according to preservation day and incubation time, respectively. The sperm motility steadily declined from 96.9% at 0.5 h incubation to 78.8% at 6 h incubation at 1 day of preservation. However, the sperm motility rapidly declined after 4 day of preservation during incubation. The normal acrosome steadily declined from 93.3% at 0.5 h incubation to 73.8% at 6 h incubation at 1 day of preservation. However, the normal acrosome rapidly declined after 3 day of preservation during incubation. The rates of sperm penetration and polyspermy were higher in 5 and $10{\times}10^6$ sperm/ml than in 0.2 and $1{\times}10^6$ sperm/ml. Mean numbers of sperm in penetrated oocyte were highest in $10{\times}10^6$ sperm/ml compared with other sperm concentrations. The rates of blastocysts from the cleaved oocytes (2-4 cell stage) were highest in $1{\times}10^6$sperm/ml compared with other sperm concentrations. In conclusion, we found out that liquid boar sperm stored at $4^{\circ}C$ could be used for in vitro fertilization of pig oocytes matured in vitro. Also, we recommend $1{\times}10^6$sperm/ml concentration for in vitro fertilization of pig oocytes.