• 한국수소및신에너지학회논문집
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• 제32권5호
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• pp.356-364
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• 2021

During the hydrogen fueling process, hydrogen temperature inside the compressed tank were limited below 85℃ due to the allowable pressure of tank material. The chiller system to cool compressed hydrogen used R407C, greenhouse gas with a high global warming potential (GWP), as a refrigerant. To reduce greehouse gas emission, it should be replaced by refrigerant with a low GWP. This study proposes a chiller system for fueling hydrogen with R290, consisted in propane, by applying the C3 pre-cooled system use d in the LNG liquefaction process. The proposed system consisted of hydrogen compression and cooling sections and optimized the operating pressure through exergy analysis. It was also compared to the exergy efficiency with the existing system at the optimal operating pressure. The result showed that the optimal operating pressure is 700 kPa in 2-stage, 840 kPa/490 kPa in 3-stage, and the exergy efficiency increased by 17%.

• Geomechanics and Engineering
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• 제19권1호
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• pp.71-78
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• 2019

Massive investments are going on to promote and build transportation infrastructure all across the globe with the challenges being more than budgetary. Sandy soils which are predominant in coastal and border areas in India have typical characteristics. The shear strength of such soil is very low which makes it difficult for any kind of geotechnical construction and hence soil stabilization needs to be carried out for such soil conditions. The use of geocells is one of the most economical methods of soil improvement which is used to increase strength and stiffness and reduce the liquefaction potential of the soil. The use of geocells in stabilizing desert sand and results from a series of plate load test on unreinforced soil and geocell reinforced homogenous sand beds are presented in the present study. It also compares the field results using various load class vehicles like heavy load military vehicles on geocell reinforced soils with the experimental results and comes out with the fact that the proposed technique increases the strength and stiffness of sandy soil considerably and provides a solution for preventing settlement and subsidence.

• Clinical and Experimental Reproductive Medicine
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• 제49권4호
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• pp.270-276
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• 2022

Objective: The present study assessed the biological characteristics of human spermatozoa at different time intervals (0, 1, 1.5, and 2 hours) after incubation at 37℃. Methods: Twenty-five normozoospermic semen samples were incubated at 37℃. Incubation was performed at four time intervals of 0 (after liquefaction), 1, 1.5, and 2 hours. The samples were evaluated for sperm parameters at each time interval. Results: The rate of sperm progressive motility decreased at 1.5 hours compared to 0 hours as well as 2 hours compared to 1 hour and 0 hours. The rate of non-motile spermatozoa also decreased after 2 hours compared to after 0 hours. No significant changes were observed in sperm viability (p=0.98) and non- progressive motility (p=0.48) at any time intervals. Abnormal sperm morphology increased at 1.5 hours of incubation time (p<0.001). No significant changes were observed in DNA fragmentation at 1 hour compared to 0 hours (median [interquartile range]: 19.5 [4] vs. 19 [4]), as well as at 1.5 hours compared to 1 hour (20 [5]). However, a significant increase in DNA fragmentation was observed at 1.5 hours compared to 0 hours. The mitochondrial membrane potential decreased remarkably after 1 hour of incubation time. No significant differences were observed in the acrosome reaction or malonaldehyde levels at any time point (p=0.34 and p=0.98, respectively). Conclusion: The incubation of normozoospermic samples before use in assisted reproductive technology should be less than 1.5 hours to minimize the destructive effects of prolonged incubation time on general and specific sperm parameters.

• Korean Chemical Engineering Research
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• 제56권1호
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• pp.49-55
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• 2018

에너지 위기 시대를 맞이하여 수소에너지가 가장 가능성 있는 대체에너지 중의 하나로 고려되고 있다. 액체수소는 기체수소와 비교하여 단위 부피당 에너지 밀도가 월등히 높으며 수소에너지의 탁월한 저장 방법으로 간주되고 있다. 본 연구에서는 2 상 모델에 기초를 둔 Navier-Stokes 식을 전산유체역학 프로그램을 이용하여 풀었으며, 초저온 냉각 튜브를 통과하면서 기체수소가 액화되는 과정을 분석하였다. 열전도율이 높은 구리관을 초저온 냉각을 위한 관의 재질로 가정하였다. 기체수소의 유입속도를 5 cm/s, 10 cm/s, 20 cm/s로 변화시키면서 냉각튜브 내 유체 온도분포, 축방향 및 반경방향 유체 속도, 기체 및 액체 수소 부피분율 분포를 각각 분석하였다. 본 연구 결과는 향후 액체수소 제조를 위한 기체수소 초저온 냉각기의 설계 및 제작을 위한 기초자료로 활용이 될 것으로 기대된다.

• 한국지반환경공학회 논문집
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• 제8권4호
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• pp.57-65
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• 2007

토목구조물의 안정성에 영향을 미치는 인자 중 지진에 의한 피해를 들 수가 있다. 최근의 지진 피해 사례를 분석하여 보면 모래와 같은 조립토뿐만 아니라 점성토, 실트 등과 같은 세립토에서도 크고 작은 피해 사례가 보고되고 있다. 이에 본 연구에서는 지진에 의한 기초지반의 강도저하를 평가하는 방법을 조사하기 위하여, 반복단순전단시험기를 이용하여 비배수상태에서 조립토 및 세립토에 반복하중을 작용시켜 지반의 강도저하특성을 조사하고 조립토의 경우에는 여유간극비, 세립토의 경우에는 소성지수를 이용하여 간단한 평가방법을 제안하였다. 분석결과에 따르면 액상화강도를 비교 분석하여 보면 조립토에서는 여유간극비의 증가에 따라 감소하고, 세립토의 경우에는 소성지수가 증가함에 따라 커지는 것을 알았다. 특히, 화강풍화토는 Collapse현상이 발생하여 다른 흙보다 현저한 강도 감소 경향이 나타났다. 간이강도저하 평가방법을 통해서 조립토와 세립토 거의 대부분이 양대수곡선상에서 -0.15 ~ -0.2의 범위내의 비슷한 기울기 내에서 분포하였지만, 화강풍화토와 같은 흙은 강도저하 경향이 -0.3으로 다른 흙보다 크게 나타남을 알 수 있었다. 본 강도저하 평가방법은 추가 연구가 필요하지만, 실무에서 간단하게 적용 가능할 것으로 보인다.

• 대한토목학회논문집
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• 제39권6호
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• pp.811-819
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• 2019

2016년 경주지진과 2017년 포항지진이 발생한 이후, 우리나라 내진설계에 있어서 다양한 실내진동시험의 수행이 증가하고 있으나, 액상화 평가나 수치해석의 입력파라미터를 산정하기 위한 실내진동시험에서는 이전부터 사용해 오던 정현하중의 이용이 계속되고 있다. 그러나, 최근 정현하중으로는 지진하중으로 야기되는 지반 내 과잉간극수압의 거동을 정확하게 모사하기 어렵다는 연구결과가 발표되며 정현하중을 대신하여 동하중의 개발에 대한 논의가 주목을 받기 시작하였다. 이 연구에서는 이러한 문제를 해결하고자 조합형 정현하중을 제안하고 주문진 표준사를 대상으로 한 진동전단실험과 진동삼축압축시험을 통해 그 적용성을 검토하였으며 추가로 진동대시험과 유효응력해석모델에 기초한 수치해석을 통해 그 타당성을 검토하였다. 검토 결과, 제안된 조합형 정현하중이 정현하중보다 실지진하중 하에서의 포화지반 내 과잉간극수압의 거동변화를 잘 모사할 수 있음을 발견하였다.

• 한국항해항만학회지
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• 제44권2호
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• pp.128-135
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• 2020

Long-term shipping contracts represent the cooperative and coexisting relationships between the shipping and steel industries. Yet, differences between the contract forms for iron ore and steel products have emerged. Specifically, the large proportion of consecutive voyage charters (CVC) is being applied in the iron ore trade, whereas the contract of affreightment (COA) is proportionally higher for shipping steel products. The literature review and in-depth interviews in this study identified through the research model, the characteristics of the shipping and market structure in both markets have significantly contributed to the preference of different long-term contracts. It has been determined that the mutual oligopoly market structure and the characteristics of shipping such as, the small number of suitable vessels in the market, the single fixed load/discharge ports, the long-distance voyages, and the potential risks for fatal accidents because of cargo liquefaction, for the iron ore trade, provide higher contribution to the preference of CVC contracts. In contrast, the consignor oligopoly market structure and the shipping characteristics, such as the greater number of suitable vessels available in the market, the variation in ports, the cargo quantity per shipment, the various load/discharge ports, and the need for experienced carriers for steel product loading in the steel product trade has shown higher preference on the COA contracts as the consignors with superiority over the shipowners, resulting in favorable contract types and conditions for the consignors.

• Journal of Microbiology and Biotechnology
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• 제31권4호
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• pp.570-583
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• 2021

Pyrococcus furiosus α-amylase can hydrolyze α-1,4 linkages in starch and related carbohydrates under hyperthermophilic condition (~ 100℃), showing great potential in a wide range of industrial applications, while its relatively low productivity from heterologous hosts has limited the industrial applications. Bacillus subtilis, a gram-positive bacterium, has been widely used in industrial production for its non-pathogenic and powerful secretory characteristics. This study was conducted to increase production of P. furiosus α-amylase in B. subtilis through three strategies. Initial experiments showed that co-expression of P. furiosus molecular chaperone peptidyl-prolyl cis-trans isomerase through genomic integration mode, using a CRISPR/Cas9 system, increased soluble amylase production. Therefore, considering that native P. furiosus α-amylase is produced within a hyperthermophilic environment and is highly thermostable, heat treatment of intact culture at 90℃ for 15 min was performed, thereby greatly increasing soluble amylase production. After optimization of the culture conditions (nitrogen source, carbon source, metal ion, temperature and pH), experiments in a 3-L fermenter yielded a soluble activity of 3,806.7 U/ml, which was 3.3- and 28.2-fold those of a control without heat treatment (1,155.1 U/ml) and an empty expression vector control (135.1 U/ml), respectively. This represents the highest P. furiosus α-amylase production reported to date and should promote innovation in the starch liquefaction process and related industrial productions. Meanwhile, heat treatment, which may promote folding of aggregated P. furiosus α-amylase into a soluble, active form through the transfer of kinetic energy, may be of general benefit when producing proteins from thermophilic archaea.

• Current Applied Physics
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• 제18권12호
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• pp.1523-1527
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• 2018

The thermal stability, magnetic and magnetocaloric properties of $Gd_{55}Co_{35}M_{10}$ (M = Si, Zr and Nb) melts-pun ribbons were studied. The relatively high reduced glass transition temperature ($T_{x1}/T_m$ > 0.60) and low melting point ($T_m$) resulted in excellent glass forming ability (GFA). The Curie temperatures ($T_C$) of melt-spun amorphous ribbons $Gd_{55}Co_{35}M_{10}$ for M = Si, Zr and Nb were 166, 148 and 173 K, respectively. For a magnetic field change of 2 T, the values of maximum magnetic entropy change $(-{\Delta}S_M)^{max}$ for $Gd_{55}Co_{35}Si_{10}$, $Gd_{55}Co_{35}Zr_{10}$ and $Gd_{55}Co_{35}Nb_{10}$ were found to be 2.86, 4.28 and $4.05J\;kg^{-1}K^{-1}$, while the refrigeration capacity (RC) values were 154, 274 and $174J\;kg^{-1}$, respectively. The $RC_{FWHM}$ values of amorphous alloys $Gd_{55}Co_{35}M_{10}$ (M = Si, Zr and Nb) are comparable to or larger than that of $LaFe_{11.6}Si_{1.4}$ crystalline alloy. Large values of $(-{\Delta}S_M)^{max}$ and RC along with good thermal stability make $Gd_{55}Co_{35}M_{10}$ (M = Si, Zr and Nb) amorphous alloys be potential materials for magnetic cooling operating in a wide temperature range from 150 to 175 K, e.g., as part of a gas liquefaction process.

• Geomechanics and Engineering
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• 제28권5호
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• pp.505-520
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• 2022

In recent years, geotextile-encased gravel columns (usually called stone columns) have become a popular method to increasing soil shear strength, decreasing the settlement, acceleration of the rate of consolidation, reducing the liquefaction potential and increasing the bearing capacity of foundations. The behavior of improved loose base-soil with gravel columns under shear loading and the shear stress-horizontal displacement curves got from large scale direct shear test are of great importance in understanding the performance of this method. In the present study, by performing 36 large-scale direct shear tests on sandy base-soil with different fine-content of zero to 30% in both not improved and improved with gravel columns, the effect of the presence of gravel columns in the loose soils were investigated. The results were used to predict the shear stress-horizontal displacement curve of these samples using support vector machines (SVM). Variables such as the non-plastic fine content of base-soil (FC), the area replacement ratio of the gravel column (Arr), the geotextile encasement and the normal stress on the sample were effective factors in the shear stress-horizontal displacement curve of the samples. The training and testing data of the model showed higher power of SVM compared to multilayer perceptron (MLP) neural network in predicting shear stress-horizontal displacement curve. After ensuring the accuracy of the model evaluation, by introducing different samples to the model, the effect of different variables on the maximum shear stress of the samples was investigated. The results showed that by adding a gravel column and increasing the Arr, the friction angle (ϕ) and cohesion (c) of the samples increase. This increase is less in base-soil with more FC, and in a proportion of the same Arr, with increasing FC, internal friction angle and cohesion decreases.