• 대한한의학회지
• /
• 제23권2호
• /
• pp.139-150
• /
• 2002

OBJECTIVES : Schizandra chinensis is well known for its efficacy at liver function reinforcement, relieving thirst and recovery from fatigue. In this study, we examined the effects of sports drink containing Schizandra chinensis on serum metabolic substrate, electrolyte, stress indicators, related-enzyme and exercise performance, rectal temperature, and heat shock proteinb70 (HSP70). METHODS : Elite long-distance runners (male, 21.3yrs, n=16) were selected and divided into two groups; an experimental group (EXP, n=8) and a control group (CON, n=8). A beverage containing Schizandra chinensis was supplemented 3 times per day to EXP for 4 weeks. Serum biochemical elements (glucose, lactate, total cholesterol, triglyceride, high density lipoprotein cholesterol, glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, creatinine, creatine phosphokinase, lactate dehydrogenase, blood urea nitrogen, Na, K, Cl) were analyzed by auto blood analyzer. Exercise performance was measured by treadmill exercise test, HSP70 was detected by electrophoresis and Western blotting, and rectal temperature was measured by rectal temperature probe. RESULTS : Administration of the beverage increased significantly the rest level of blood Na, Cl and glucose and decreased significantly lactate dehydrogenase, glutamate oxaloacetate transaminase. No difference was found in exercise performance, rectal temperature increment or HSP70 concentration between groups. CONCLUSIONS : Administration of a sports drink containing Schizandra chinensis altered blood glucose, lactate dehydrogenase, glutamate oxaloacetate transaminase, Na and Cl levels.

• Tuberculosis and Respiratory Diseases
• /
• 제57권4호
• /
• pp.377-380
• /
• 2004

유미흉은 주로 악성종양에 의한 흉관의 폐쇄, 유미뇨는 림프관과 요관과의 누공형성이 주된 원인이다. 저자들은 유미흉의 원인으로 섬유육종에 의한 흉관폐쇄, 유미뇨의 원인으로 요로계와 림프관의 누공형성을 의심하였으나 검사상 증거를 찾을 수 없어 특발성 유미흉 및 특발성 유미뇨로 진단한 1예를 문헌고찰과 함께 보고하는 바이다.

• 한국응용과학기술학회지
• /
• 제3권2호
• /
• pp.35-40
• /
• 1986

자동산화유의 독성을 구명하기 위하여 autoxidized methyl linoleate(AOML)의 일정량을 mouse에 1 일 1 회 20 일간 경구 투여하여 혈청 및 간의 지질대사에 미치는 영향을 검토한 결과는 다음과 같다. 실험군의 혈청 TBA값과 혈청 triglyceride, phospholipid 및 total cholesterol양은 정상군에 비해 모두 증가되었으며, 전기영동 결과 혈청 lipoprotein의 조성 백분율은 albumin이 약 21% 증가된 반면, VLDL은 약 70% 그리고 LDL은 약 33% 감소되어 있었다. 실험군의 간에서의 POV, COV 및 TBA값은 모두 정상군에 비해 크게 증가하였으며, 또한 간의 triglyceride, phospholipid 및 total cholesteroldml 양도 증가하는 경향을 나타내고 있었다. 한편, 실험군의 간에서의 형태학적 변화로는 정상군에 비해 간세포의 손상과 지질의 축적이 관찰 되었다.

• Journal of Microbiology and Biotechnology
• /
• 제17권6호
• /
• pp.934-944
• /
• 2007

Paenibacillus polymyxa E681 is known to be able to suppress plant diseases by producing antimicrobial compounds and to promote plant growth by producing phytohormones, and secreting diverse degrading enzymes. In spite of these capabilities, little is known regarding the flow of information from the bacterial strain to the barley roots. In an attempt to determine the flow of information from the bacterial strain to barley roots, the strain was grown in the presence and absence of barley, and two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and MALDI-TOF mass spectrometry were used. 2D-PAGE detected approximately 1,000 spots in the cell and 1,100 spots in the supernatant at a pH 4-10 gradient. Interestingly, about 80 spots from each sample showed quantitative variations. Fifty-three spots from these were analyzed by MALDI-TOF mass spectrometry and 28 proteins were identified. Most of the cytosolic proteins expressed at higher levels were found in P. polymyxa E681 cells grown in the presence of barley rather than in the absence of barley. Proteins detected at a lower level in the surpernatant of P. polymyxa E68l cells grown in the presence of barley were lipoprotein, glucose-6-phosphate 1-dehydrogenase, heat-shock protein HtpG, spermidine synthase, OrfZ, ribonuclease PH, and coenzyme PQQ synthesis protein, and flagellar hook-associated protein 2 whereas proteins detected at a higher level in the surpernatant of P. polymyxa E681 cells grown in the presence of barley included D-alanyl-D-alanine ligase A, isopentenyl-diphosphate delta-isomerase, ABC transporter ATP-binding protein Uup, lipase. Many of the proteins belonging to plant-induced stimulons are associated with biosynthetic metabolism and metabolites of proteins and transport. Some of these proteins would be expected to be induced by environmental changes resulting from the accumulation of plant-secreted substances.

• 한국식품과학회지
• /
• 제30권5호
• /
• pp.1214-1221
• /
• 1998

지질 산화에 의해 생성하는 MDA (malondialdehyde)를 thiobarbituric acid와 반응시켜 측정한 MDA 값은 곰취 추출물의 농도가 증가함에 따라 감소하므로서 추출물들에 의해 산화가 억제됨을 알 수 있었다. 메탄올 추출물의 경우 $15\;{\mu}g/mL$농도로 첨가시 71.7% (13.36 nmol/㎎)의 산화억제율을 나타내었으며 분획물의 경우 ethyl acetate 분획물이 동일농도에서 97% (1.35 nmol/mg)의 억제율을 나타내었다. 산화시간에 따른 MDA 값의 변화는 $25\;{\mu}g/mL$의 농도를 첨가시 에탄올, 메탄올 및 물 추출물의 경우 4시간까지 산화를 억제하였으며 분획물의 경우에는 $20\;{\mu}g/mL$의 농도를 첨가시 ethyl acetate 분획물이 8시간까지 강한 항산화효과를 나타내었다. Agarose gel electrophoresis에서는 에탄올, 메탄올 그리고 물 추출물 모두 항산화 효과가 인정되었다. 지질산화에 의해 생성되는 conjugated diene은 곰취 추출물의 경우 $Cu^{2+}$로 산화시켰을 때 대조에 비해 약 1.1배에서 2.8배까지 감소시키는 효과를 나타내었고 분획물의 경우 약 2.2배에서 3.2배까지의 감소효과를 나타냄으로서 강한 산화 억제효과를 나타내었다. SDS-PAGE를 이용하여 LDL내부에 존재하는 apo B-100부분에 대하여 산화에 따른 분해정도를 측정한 결과 에탄올, 메탄올 및 물 추출물이 천연 LDL의 band와 유사한 band를 나타내었다. Densitometer를 이용한 apo B-100함량은 천연 LDL의 함량을 100%로 하였을 때 에탄올, 메탄올 및 물 추출물의 경우 각각 77.8, 92.5 그리고 82.3%의 함량을 나타내었다. 분획물의 경우 헥산, ethyl acetate 및 물 분획물에서 각각 38.8, 94.5% 그리고 65.5%의 함량을 나타냄으로서 ethyl acetate 분획물이 강하게 산화를 억제함으로서 apo B-100의 분해를 막아주고 있음을 알 수 있었다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제32권7호
• /
• pp.922-929
• /
• 2019

Objective: Mutations in low-density lipoprotein receptor (LDLR), which encodes a critical protein for cholesterol homeostasis and lipid metabolism in mammals, are involved in cardiometabolic diseases, such as familial hypercholesterolemia in pigs. Whereas microRNAs (miRNAs) can control LDLR regulation, their involvement in circulating cholesterol and lipid levels with respect to cardiometabolic diseases in pigs is unclear. We aimed to identify and analyze LDLR as a potential target gene of SSC-miR-20a. Methods: Bioinformatic analysis predicted that porcine LDLR is a target of SSC-miR-20a. Wild-type and mutant LDLR 3'-untranslated region (UTR) fragments were generated by polymerase chain reaction (PCR) and cloned into the pGL3-Control vector to construct pGL3 Control LDLR wild-3'-UTR and pGL3 Control LDLR mutant-3'-UTR recombinant plasmids, respectively. An miR-20a expression plasmid was constructed by inserting the porcine premiR-20a-coding sequence between the HindIII and BamHI sites in pMR-mCherry, and constructs were confirmed by sequencing. HEK293T cells were co-transfected with the miR-20a expression or pMR-mCherry control plasmids and constructs harboring the corresponding 3'-UTR, and relative luciferase activity was determined. The relative expression levels of miR-20a and LDLR mRNA and their correlation in terms of expression levels in porcine liver tissue were analyzed using reverse-transcription quantitative PCR. Results: Gel electrophoresis and sequencing showed that target gene fragments were successfully cloned, and the three recombinant vectors were successfully constructed. Compared to pMR-mCherry, the miR-20a expression vector significantly inhibited wild-type LDLR3'-UTR-driven (p<0.01), but not mutant LDLR-3'-UTR-driven (p>0.05), luciferase reporter activity. Further, miR-20a and LDLR were expressed at relatively high levels in porcine liver tissues. Pearson correlation analysis revealed that porcine liver miR-20a and LDLR levels were significantly negatively correlated (r = -0.656, p<0.05). Conclusion: LDLR is a potential target of miR-20a, which might directly bind the LDLR 3'-UTR to post-transcriptionally inhibit expression. These results have implications in understanding the pathogenesis and progression of porcine cardiovascular diseases.

• 한국잠사곤충학회지
• /
• 제30권1호
• /
• pp.25-32
• /
• 1988

누에 유충에 있어서 체액 JH 결합단자질(JHBP)의 생리적 역할을 알기 위하여, 체액 중의 JH 1과 결합하는 단자질을 polyacrylamide gel 전기영동으로 분리하고, 또 발육에 따른 JH 결합단자질의 활성 변화를 측정한 결과는 다음과 같다. 1. 누에 체액중에서 JH 1과 결합하는 단자질은 JH 결합지질단자질(JH-LP)과 Jh 결합단자질(JHBP) 등 JH결합단자질의 Rm은 0.81∼0.33이었고, JH 결합단자질의 Rm은 0.81∼0.84이었다. 두 단자질의 Rm은 성별, 발육시기에 관계없이 일정하였다. 2. 체액단자질의 함량은 발육의 진행에 따라 점차 증가하여 토사기에 가장 높았으며 전용기에 다소 감소하였고 암컷의 함량은 숫컷보다 높았다. 3. 체액 ml 당 JH 결합단자질의 활성은 영의 초기에 낮았고 토사기에 최대로 나타난 후 전용기에 다소 감소하였다. 4. 체액 ml 당 JH 결합단자질의 활성 변화는 체액단자질의 함량의 변화양상과 매우 유사하였다. 5. 체액단자질 mg 당 JH 결합단자질의 활성은 영의 초기에 높았고 5령 6일에서 전용기까지 낮은 수준을 유지하였다.