• Proceedings of the PSK Conference
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• 2000.04a
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• pp.109.1-109.1
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• 2000

• Journal of Food Hygiene and Safety
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• v.36 no.6
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• pp.528-536
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• 2021

The purpose of the study was to investigate the content of sinigrin, an index component, in Brassica juncea extract and to evaluate the differentiation of lipocytes, inhibition of production of reactive oxygen species (ROS) and reduction of protein production by lipogenic factors (PPARγ, C/EBPα, aP2) in the processing of Brassica juncea extract and sinigrin in 3T3-L1 preadipocytes which induces Bisphenol A (BPA), an endocrine disrupting environmental hormone. From the investigation, the content of sinigrin in Brassica juncea extract, measured by HPLC, is found to be 21.27±0.2 mg/g. The XTT assay result on BPA-derived 3T3-L1 adipocytes shows there is no cytotoxicity found from 180 µM of sinigrin and 300 ㎍/mL of Brassica juncea extract. Moreover, both intracellular lipid accumulation and ROS production during differentiation of lipocyte are significantly reduced in cells processed with Brassica juncea extract and sinigrin. Lastly, it was also found that the production of transcription factors of lipocyte differentiation, PPARγ, C/EBPα and aP2, were found to be suppressed by the application of Brassica juncea extract and sinigrin. Such results reveals that Brassica juncea is effective in not only suppressing lipid accumulation in the environmental hormone bisphenol A-derived lipocyte, but also in reducing the ROS. The sinigrin-containing Brassica juncea is highly expected to be used in natural functional supplements that prevents the lipid metabolism disorders caused by BPA. There are necessities for additional clinical research and follow-up studies on the in vivo model to verify the relevant mechanisms.

• Journal of Veterinary Clinics
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• v.28 no.4
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• pp.457-459
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• 2011

A dome-shaped subcutaneous nodular mass was detected on the forehead of 2-year-old mixed cattle encountered at Jeju slaughter house. The mass showed distinctive greasy appearance and float in 10% buffered formalin. Mass was well demarcated with other tissues, and firm in consistency and whit-yellowish color on cut surface. Histopathologically, subcutaneous mass was well circumscribed but unencapsulated. This mass was composed of larger lipocytes or more variability in size than normal mature lipocytes. The cytoplasmic lipid of tumor cells could be demonstrated using Oil red O staining. Based on the gross and histopathologic findings, this case was diagnosed as subcutaneous lipoma in cattle. In our best knowledge, this is the first case of bovine lipoma in Korea.