• 한국수산과학회지
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• 제27권1호
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• pp.69-82
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• 1994

해조류를 첨가한 사료가 조피볼락의 어린 시기에 미치는 생리적 효과를 알아보기 위하여, 전장 $2.99{\pm}0.23\;cm$, 체중 $1.03{\pm}0.18\;g$의 인공종묘에게 미역분말을 각각 $3\%,\;5\%,\;7\%$ 첨가한 사료와 비첨가 사료를 주면서 140일간 사육하여 전장$18.52{\pm}1.07\;cm$, 체중 $120.64{\pm}19.32\;g$이 될 때까지의 어체 성장, 생존율, 체조성, 혈액조성을 서로 비교하고, 저산소 및 기아 내성에 관한 생리활성을 연구하였다. 1. 전장과 체중의 성장은 $5\%$의 미역 첨가시 가장 좋았으며, $3\%,\;7\%$, 대조구인 $0\%$ 순이었다. 2. 실험구별 사료효율은 $3\%,\;5\%,\;7\%,\;0\%$구의 순으로 높았으며, 생존율은 $7\%,\;5\%,\;3\%,\;0\%$구 순이었다. 3. 사육종료시의 체조성에 있어 등 근육의 수분, 조단백질, 조지방 및 조회분은 실험구별로 큰 차이를 보이지 않았으나, 전내장의 조지방 함량은 미역 첨가구가 대조구에 비해 많았다. 4. 미역 첨가구는 $0\%$구 보다 헤마토크릿트값이 크고, 평균적혈구수도 많았다. 5. 실험종료시 사료별 어체의 저산소내성은 미역 첨가구가 대조구에 비해 강했다. 6. 사육실험 종료후 31일간의 기아 실험에서 미역 첨가구의 어체중은 대조구에 비해 적게 감소하였다. 7. 조피볼락 양식에서 사료에 미역을 첨가해 줄 경우, 그 비율은 $5\%$가 가장 적합하며 어체의 생리조건에 좋은 영향을 줄 수 있는 것으로 판단되었다.

• 한국수산과학회지
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• 제29권3호
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• pp.393-408
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• 1996

우렁쉥이 껍질 색소추출물을 astaxanthin 함량으로 환산하여 100ppm, 200ppm 및 400ppm 첨가 한 후 보리새우를 8주간 사육하여 성장도, 착색정도, 지질 및 총 콜레스테롤 함량 그리고 육과 머리의 지방산조성을 조사하였다. 성장율은 대조구(Diet 1)가 가장 낮아 0.610이었고, carophyll pink 첨가구(Diet 2)가 1.065, 껍질 색소추출물 첨가구인 Diet 3(100ppm), Diet 4(200ppm), Diets (400ppm)는 각각 1.227, 1.292, 1.264로 Diet 4 첨가구가 가장 양호하였다. 총카로테노이드 함량은 대조구가 8주 후 육 및 껍질 중 0.176mg, 1.429mg으로 감소하였으나, 색소추출물구인 Diet 4와 5는 각각 2.337mg, 6.331mg으로 가장 우수하였다. Astaxanthin monoester가 free형보다 착색에 더 영향을 미치는 것으로 나타났다. 육 및 머리 중 지질함량도 Diet 4, 5가 8주 후 각각 1632.7mg, 1691.4mg, 1973.5mg, 2071.1mg으로 가장좋았고, 육 및 머리의 콜레스테롤 축적량은 Diet 2가 8주 후 각각 481.5mg, 199.5mg이었으나, Diet 4는 각각 978.2mg, 563.8mg으로 성장에 직접 영향을 미치는 것으로 나타났다. 머리 지질 중 총지질(TL) 및 중성지질(NL)의 지방산조성은 모노엔산의 함량이 높고 고도불포화지방산(PUFA) 함량은 낮았으나, 이와는 반대로 국성지질(PL)은 모노엔산 함량은 낮고 PUFA 함량은 높아 인지질 중 n-3계 고도불포화지방산의 비율이 높게 나타나 색소추출물이 필수지방산 공급원의 역활을 하는 것으로 나타났다.

• 한국식품영양과학회지
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• 제30권6호
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• pp.1197-1203
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• 2001

고콜레스테롤 식이 흰쥐에서 xylooligo당이 간조직중의 UDP-glucronyl transferase 활성과 분변을 통한 지질, 담즙산 및 중성 sterol류의 배설 및 장기능개선 효과를 관찰하였다. 실험동물은 100$\pm$10g의 Sprague-Dawley 횐쥐를 이용하여 정상 식이군과 1% 고콜레스테롤 실험군으로 나누고 다시 실험군은 1%의 고콜레스테롤 식이에 xylooligo당을 0%, 5%, 10% 및 15% 농도 첨가군(C, C5XO, C10XO 및 C15XO군)로 나누어 각군마다 10마리씩 4주간 자유섭식 시켰다. 분변중의 총지질 및 TG함량은 C군에 비해 xylooligo당 공급군에서 현저하게 증가되었다. 간 microsome의 UDP-glucuronyl transferase의 활성은 정상군에 비해 C군에서 35% 증가되었고 또 C5XO, C10XO 및 X15XO군은 C군에 비해 15%, 41% 및 21%씩 각각 증가되었다. 분변중의 bile acid 함량은 C군에 비해 C5XO, X10XO 및 C15XO군에서 3.1배, 3.6배, 2.8배씩 각각 증가되었다. 분변중 중성 스테롤류인 cholesterol, coprostanol, coprostanone 함량은 C군에 비해 xylooligo당 공급군에서 현저하게 증가되었다. 이상의 결과에서 xylooligo당은 고콜레스테롤 식이 횐쥐에서 간조직의 UDP-GTase활성을 증가시키고 분변으로 총지질 및 중성지방 그리고 스테롤류의 배설을 증가시키는 효과가 있었고 또 분변량도 증가시키는 장기능 개선 효과도 뚜렷하였다.

• 한국수산과학회지
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• 제56권6호
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• pp.781-789
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• 2023

We investigated the sensory and quality characteristics of gambas al ajillo retort foods with nondigestible maltodextrin (trial gambas) compared with commercially available frozen gambas al ajillo (control). Trial gambas demonstrated approximate compositions of 75.8% moisture, 11.3% protein, 5.8% lipid, 1.7% ash, and 5.4% carbohydrates, indicating lower lipid and carbohydrate contents but higher moisture, protein, and ash levels than that of the control. Sensory assessments conclusively established the superior overall acceptability of trial gambas over the control. Trial gambas exhibited a total amino acid content of 9.62 g/ 100 g, surpassing the 5.39 g of the control. The major amino acids, such as lysine, leucine, arginine, aspartic acid, and glutamic acid, contributed significantly to its enhanced nutritional profile. Mineral analysis revealed higher calcium, phosphorus, magnesium, and iron levels in trial gambas and lower potassium levels than that in the control. Fatty acid profiling identified 18:1n-9 (67.8%) and 16:0 (11.9%) as the predominant fatty acids, in concordance with the control. This study substantiates trial gambas with the health-oriented preferences of the MZ generation while presenting superior sensory and quality attributes compared with the control.

• Journal of Nutrition and Health
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• 제24권3호
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• pp.179-188
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• 1991

Twenty college women were led experimental diet which composed ot basal diet plus different kinds of dietary rats at 27% Cal. Equal amount of 13.5g of corn oil, perilla oil or fish oil was supplied for 2 weeks as a source of n6 linoleic acid(LA). n3 $\alpha$-linolenic acid (LL). or n3 EPA + DHA. respectively. Plasma total Chol level was reduced by perilla and fish oils, significantly only by fish oil. Plasma Chol level was rather increased by corn oil(P<0.05), but was decreased by double amount of corn oil supplement. Therefore, hypocholesterolemic effect of fatty acids was in the order of n3 EPA+DHA>n3 LL>n6 LA and influenced by the degree of fat unsaturation. Plasma TG level was also significantly decreased by n3 EPA+ DHA and increased by n6 LA. Hypotriglyceridemic effect of fatty acids was also in the order of n3 EPA + DHA> n3 LL>n6 LA and influenced by the unsaturation. However, the reduction of plasma TG was more influenced by the fatty acid structure rather than the fat unsaturation. There were no significant effects on lipoprotein pattern 3nd chemical compositions of lipoprotein by different dietary PUFAs. but fish oil diet significantly increased the relative proportion of HDL-Chol. In conclusion. cholesterol- lowering effect of dietary PUFAS seemed to be a function of total fat unsaturation but hypotriglyceridemic effect seemed to be more linked to the ratty acid structure rather than the degree of unsaturation. The hypolipidemic effect of n3 PUFAs was significant so that fish oil or perilla oil may have important nutritional applications in the prevention and treatment of atherosclerotic disease.

• 한국식품조리과학회지
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• 제17권1호
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• pp.80-90
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• 2001

This study was carried out to investigate to proximate compositions, free sugars, organic acids, amino acids, and volatiles from the fresh leaf, root and seed of coriander. The research results are as follows: Moisture was 79.93% in the leaf, 81.89% in the root. Crude protein, crude lipid and crude ash were the highest in the seed. Ascorbic acid was 65.4mg% in the leaf and 37.83mg% in the root. Glucose, fructose and sucrose were the major free sugars. Glucose was 7.92mg% and fructose 7.51mg% in the leaf. Sucrose was 17.34mg% in the root, highest level. Among organic acids, malic acid was 354.55mg% in the leaf, the highest level. The content rate of organic acids was high in the order of leaf, seed and root. The content rate of total amino acid was high in the order of seed, root and leaf. Glutamic acid and aspartic acid were high in the leaf and root. Glutamic acid and proline were high in the seed. The content rate of free amino acid is the same as that of total amino acid. Glutamic acid and serine were high in the leaf and seed. Glutamic acid and treonine were high in the root. The contents of total amino acid in each parts of the coriander was higher than that of free amino acid, The composition of amino acid in the total amino acid and free amino acid was different. The volatile constituents were extracted by steam distillation method and analyzed by GC-Mass. The content of the volatile constituents was 45.31mg% in the leaf, (E)-2-decenal was the highest, followed by decanal, 2-dodecenal, (E)-2-decen-1-ol in order, aldehyde and alcohol was major constituents. The content of the volatile constituents was 36.01mg% in the root and 54.37mg% in the seed. linalool was the highest in the root and seed. it was 22.27 %, 53.67% in root and seed.

• 한방재활의학과학회지
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• 제20권4호
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• pp.197-213
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• 2010

Objectives : The clinical trial was designed to investigate the safety and effects of Taeeumjowui-tang(Taiyintiaowei-tang) extracts on the change of the weight, body compositions, serum in obese patients. Methods : This was a 12-weeks, randomized, double blind, placebo-controlled clinical trial. Patients with a body mass index of either greater than $30kg/m^2$ or between 27 and $30kg/m^2$ with controlled hypertension, diabetes, hyperlipidemia were considered eligible. Participants of 41 obese patients were randomized either to Taeeumjowui-tang(Taiyintiaowei-tang)(23) or placebo(18). After 12 weeks of treatment, we measured anthropometric factors(weight, height, waist circumference, BMI(body mass index) etc.), abdominal fat area by CT(computed tomography)scanning, serum lipid(total cholesterol, tryglyceride, HDL-cholesterol, LDL-cholesterol), blood lever of variety(AST, ALT, BUN, creatinine etc.). Adverse events also evaluated. Results : After 12 weeks of treatment mean weight, waist-circumference, total cholesterol, LDL-cholesterol and score of KEAT-26 were significant changed in Taeeumjowui-tang(Taiyintiaowei-tang). There were no serious adverse events in either groups. Conclusions : There were limited in this study that is conducted within a short period of 12 weeks. but its weight and WC(waist circumference), WHR(waist hip ratio), total cholesterol, LDL-cholesterol and score of KEAT-26 loss effect was significant and it had few adverse events.

• Asian-Australasian Journal of Animal Sciences
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• 제16권7호
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• pp.1003-1010
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• 2003

The effects of different rumen protected forms, oleamide, Ca oleate, of dietary oleic acid on the carcass quality and fatty acid composition in intramuscular and subcutaneous fat tissues of Hanwoo steer were examined. Sixty, 25 month old Hanwoo steers divided into three groups were fed no supplement (Control), 2% of oleamide (Oleamide) or Ca-oleate (Ca-Oleate) in their diet for 45 or 90 days. Disappearance rates of oleic acid supplements in digestive tracts (Rumen bypass, abomasal and intestinal disappearance rate) were 48.5, 68.4 for oleamide and Ca oleate, respectively. Both oleic acid supplements affected feed intake, growth rate, cold carcass weight and carcass fatness. Live weight gain, carcass weight, backfat thickness and marbling score were higher in the oleic acid supplemented steers compared with those from the control. Oleic acid supplements increased marbling score and ether extract in Hanwoo steer m. logissi thoracicmus. Rumen protected oleic acid increased not only the level of oleic acid but also polyunsaturated fatty acids in intramuscular and subcutaneous fat tissue. Total saturated fatty acid contents in both fat tissues were decreased whereas total unsaturated fatty acid content was increased compared with those from control. Linoleic acid, linolenic acid and polyunsaturated fatty acid contents were significantly higher in Ca oleate than any other steers. Lipid metabolites in blood were increased in rumen protected oleic acid treatments. HDL content in blood was increased in Ca-oleate supplemented steers whereas LDL was decreased compared with control. The changes of fatty acid compositions in the rumen protected oleic acid supplemented steers suggest that the oleic acid and unsaturated fatty acid were protected from rumen biohydrogenation and can be deposited in the fat tissues.

• Journal of Microbiology and Biotechnology
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• 제25권5호
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• pp.723-731
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• 2015

Microalgae hold promise as producers of sustainable biomass for the production of biofuels and other biomaterials. However, the selection of strains with efficient and robust production of desirable resources remains challenging. In this study, we isolated a green microalga from Korea and analyzed its morphological, molecular, and biochemical characteristics. Microscopic and phylogenetic analyses demonstrated that the isolate could be classified into the genus Chlamydomonas, and we designated the isolate Chlamydomonas sp. KIOST -1. Compositions of protein, lipid, and carbohydrate in the microalgal cells were estimated to be 58.8 ± 0.2%, 22.7 ± 1.2%, and 18.5 ± 1.0%, respectively. Similar to other microalgae belonging to Chlorophyceae, the dominant amino acid and monosaccharide in Chlamydomonas sp. KIOST-1 were glutamic acid and glucose. On the other hand, the proportions of saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids clearly differed from other species in the genus Chlamydomonas, and monounsaturated fatty acids accounted for a large portion (41.3%) of the total fatty acids in the isolate. Based on these results, Chlamydomonas sp. KIOST-1 has advantageous characteristics for biomass production.

• 대한임상검사과학회지
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• 제45권3호
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• pp.114-119
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• 2013

Potassium is essential for the proper functioning of the ears. The inner ear's endolymph differs from all other extracellular fluids (in its positive potential) and in the ionic compositions in the various parts of the endolymphatic space. Ion concentration of the endolymph is 150 mM of potassium, which is comparable to the concentrations in other organs. Cisplatin (cis-diamminedichloroplatinum II: CDDP) is one of the most effective anticancer drugs, widely used against various tumors. However, its clinical use is limited by the onset of severe side effects, including ototoxicity and nephrotoxicity. For ototoxicity, a number of evidences in cytotoxic mechanism of cisplatin, including perturbation of redox status, increase in lipid peroxydation, and formation of DNA adduct, have been suggested. Therefore, in this study, the author investigated the relationship between the potassium ions on cisplatin-induced cytotoxicity in HEI-OC1 cells associated with reactive oxygen species (ROS). KCNE1 gene expression by the concentration of intracellular potassium appeared in the plasma membrane and increased the concentration of intracellular potassium. Cisplatin decreased the viability of HEI-OC1 cells, but the KCNE1 gene increased. Also, the KCNE1 gene significantly suppressed generation of intracellular ROS by cisplatin. Western blot analysis showed that the KCNE1 gene increased phase II detoxification enzymes markers such as superoxide dismutase 1 (SOD1), superoxide dismutase (SOD2), NAD(P)H:quinine oxidoreductases (NQO1), which were associated with the scavenger of ROS. These results suggest that the KCNE1 gene for intracellular potassium concentration ultimately prevents ROS generation from cisplatin and further contributes to protect auditory sensory hair cells from ROS produced by cisplatin.