• Journal of Nutrition and Health
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• 제26권2호
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• pp.131-144
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• 1993

본 실험은 식이내 지방의 종류와 수준이 흰쥐의 LCAT활성 및 콜레스테롤 대사에 어떻게 영향을 미치는가를 알아보기 위하여 실시되었다. 식이지바의 종류는 정어리유($\omega$3 linolenic acid) 및 옥수수기름 ($\omega$6 linolic acid)의 네 처리로 나누고 각 처리마다 지방의 수준을 총 에너지의 10%, 20%, 40%등 세수준으로 모두 12군을 두었으며, 식이지바의 무첨가군을 대조구로 두었다. 총 78마리의 Sprague-Dawley 계통 흰쥐 수컷에게 시험식이를 6주간 자유급여하였다. 사양시험 종료 후 간조직에서의 콜레스테롤 합성능력을 알아보기 위해 흰쥐의 간조직을 채취하여 in vitro 배양시험을 실시하였다. 본 시험에서 얻어진 결과를 요약하면 다음과 같다. 1) 혈장 총 콜레스테롤, HDL-콜레스테롤. LDL-콜레스테롤농도 및 HDL-C/T-C비율은 식이지방의 종류에 의해 유의하게(p<0.001) 영향을 받았다. ($\omega$3지방산을 많이 함유한 군(정어리유와 들기름)이 ($\omega$6지방산과 포화지방산을 많이 함유한 군(옥수수기름과 우지)에 비해 혈장내 HDL-콜레스테롤농도가 유의성있게(p<0.001)증가하였고, LDL-콜레스테롤농도는 유의성있게(p<0.05) 감소하였다. 혈장내 콜레스테롤농도는 우지군이 다른지방군에 비해(p<0.001),무지방식이군이 식이지방첨가군에 비해 유의하게(p<0.05)영향을 받았다. 2) 혈장 lecithiin : cholesterol acyltransferase(LCAT) 활성은 식이지방첨가군이 무지방식이군보다(p<0.001), 식물성유지군이 동물성지방군보다(p<0.001), 정어리유군이 우지군보다(p<0.001), 들기름군이 옥수수기름군보다(p<0.05), 정어리유군이 들기름군보다 유의성있게(p<0.05) 증가하였다. 저지방식이군이 중지방식이군과 고지방식이군에 비해 혈장내 LCAT 활성이 유의하게9p<0.001) 증가하였다. 3) 간의 콜레스테롤합성 능력은 정어리유군이 다른지방군보다(p<0.001), 무지방식이군이 식이지방첨가군보다(p<0.001), 동물성지방군의 식물성유지군보다 유의하게(p<0.001) 증가하였으나, 식이 지방의 수준에 의한 차이는 나타나지 않았다.

• Journal of Pharmaceutical Investigation
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• 제38권1호
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• pp.39-43
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• 2008

Cyclosporin A (CyA), a potent immunosuppressive drug used in allogeneic transplants and autoimmune disease, is a typical water-insoluble drug. Recently, nanoparticle carriers were investigated to improve the intestinal absorption of drugs. In this study, CyA-loaded nanostructured lipid carriers (NLCs) were prepared from a hot o/w emulsion using the high pressure homogenization method. The NLCs were consisted of cationic lipids, solid lipids, liquid lipids (oils), surfactant and stabilizer. Encapsulation efficiency of CyA in NLCs was approximately 71%. The average particle size and zeta potential of NLCs were below 250 nm and above +40 mV, respectively. The morphology of NLCs was confirmed by transmission electron microscopy (TEM) analysis. Compared to the CyA powder, higher in vitro release of CyA from NLCs was observed after burst release within 30 min. Thus, CyA-loaded NLCs could be applied not only for parenteral route but also for gastrointestinal administration, which needs further investigation.

• 생명과학회지
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• 제12권3호
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• pp.233-241
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• 2002

전남 여수지역의 특산품이라 할 수 있는 갯장어의 연중 지질성분의 변화를 살펴보기 위해 일정 시기별로 채취한 갯장어의 일반성분 및 근육지질의 각 획분별 지방산 조성을 분석, 검토하였다. 조지방 함량은 3.85∼12.59 g/100 g의 범위로 11월에 그 함량이 가장 많았고, 5월에 가장 낮았다. 수분함량은 지방함량이 많은 시기에 적게 나타나 역의 관계를 나타내었다. 총지질 및 중성지질의 주요 구성지방산은 C16:0, C23:0, Cl6:1, Cl8:1, C20:5 및 C22:6이었으나 중성지질은 총지질에 비해 C22:6의 구성비율이 특히 낮았다. 인지질의 주요 구성지방산도 총지질이나 중성지질과 유사하였으나 Cl8:3n-6의 함량이 높은 것이 특징적이었고, 폴리엔산의 총함량(73.93∼66.23%)은 다른 지질획분에 비해 가장 높았다. 당지질의 경우는 C20:1 및 Cl4:1의 함량이 특이적으로 매우 높았으나 EPA와 DHA의 함량은 낮았다. 총지질, 중성지질, 인지질 및 당지질의 연평균 n-3/n-6 비는 각각 10.82, 12.27, 6.63 및 6.50이었다. 총지질과 중성지질의 지방산의 연중변화는 뚜렷한 경향을 찾을 수 없었으나, 인지질의 경우는 조지방 함량이 많은 9월과 11월에 폴리엔산의 함량이, 당지질에 있어서는 9, 11 및 1월에 모 노엔산의 함량이 유의적으로 낮았다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.434-437
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• 2001

배양 조건으로 온도는 4, 11, 18, 24, $32^{\circ}C$로 배양하여, $24^{\circ}C$에서 Lipid 내에 약 42.3%의 DHA 최대 수율을 보였으며, 교반속도는 50, 100, 150, 200rpm 으로 실험하여 l00rpm에서 최대수율로 42.1% 올 얻었다. 초기 당농도는 5, 8, 11, 14, 17, 23, 29, 35g/L로 주어 8g/L에서 Lipid 내 DHA 농도가 43.1%로 나타났으며, N/C 비율은 glucose 5 g/L로 고정하여0.1, 0.2, 0.4, 0.6, 0.8 에서 38-40 % 로 거의 일정하게 나타났다.

• Journal of Pharmaceutical Investigation
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• 제40권spc호
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• pp.63-73
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• 2010

Solid lipid nanoparticles (SLNs) have emerged to combine the advantages of polymeric nanoparticles and lipid emulsions in early 1990s. SLNs can present several desirable properties derived from the solid state core. When formulating SLNs, there should be careful considerations about the physical state of the inner solid lipid core and its polymorphism and supercooling behavior. In this review, SLNs were compared to lipid emulsion and emulsion of supercooled melt to understand the unusual behaviors compared to lipid emulsions and to have insights into stability and release mechanism. SLNs have been regarded as biocompatible system because lipids are usually well-tolerable ingredients than polymers. Several studies showed good tolerability of SLNs in terms of cytotoxicity and hemolysis. Similar to various other nanoparticulate drug delivery systems, SLNs can also change biodistribution of the incorporated drugs in a way to enhance therapeutic effect. Most of all, large scale production of SLNs was extablished wihtout using organic solvents. Although there is no SLN product in the market till date, several advantagious properties of SLNs and the progress we have seen so far would make commercial product of SLNs possible before long and encourage research community to apply SLN-based formulations for water-insoluble drugs.

• KSBB Journal
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• 제13권1호
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• pp.65-70
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• 1998

지질 생산 효모인 Rhodotorula glutinis K -501이 생산하는 세포외지질(extracellular lipid)의 물리화학적 성질을 조사하여 생체 계면활성제로서의 응용 가능성을 검토하였다. 분석실험 결과 생산된 세포외지질은 당지질계 물질임을 알 수 있었다. 세포외지질의 임계미셀농도와 표면장력은 각각 89mg/L와 31dyne/cm로서 계면활성제로서 매우 우수한 표면활성을 가지고 있었으며 넓은 범위의 pH에서 표면장력의 값은 일정하였다. 상용 계면활성제인 Tween 80과 Triton X-100에 비하여 세포외지질은 각각2-3배 높은 유화도와 1.3배 높은 분산력을 가지고 있었다.

• 한국임상약학회지
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• 제16권1호
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• pp.46-51
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• 2006

Dyslipidemia is an important CHD risk factor in diabetic patients. We conducted this study to assess the pattern of dyslipidemia in type 2 diabetes patients, to examine the demographic and clinical factors associated with dyslipidemia and to evaluate attaining within the lipid target goals and treatment strategies. A retrospective analysis was conducted among patents diagnosed type 2 diabetes at outpatient clinic in endocrinology between January 2003 and December 2004. Clinical history and physical examination were reviewed and laboratory data including blood glucose, HbAlc, lipid levels were recorded sequentially at least 1 year. In 882 patients with type 2 diabetes, 437 patients (49.6%) have dyslipidemia and 73% of them (319 patients) received lipid-lowering agents. 244 patients (94 males, 150 females, mean age 60 years old) were susceptible to analyses. The most frequent pattern of dyslipidemia is high LDL level and high TG levels (28%). Metabolic syndrome and macrovascular complication were significant negative independent association with lipid levels within the target goals (p<0.05). Only 15.2% (19 males, 18 females) attained within the lipid tar- get goals. Patients with diabetic dyslipidemia need maximization of lipid-lowering agents, increasing the fibric acid derivatives prescription and the effort to correction of low HDL and/or high TG.

• Fisheries and Aquatic Sciences
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• 제18권4호
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• pp.359-365
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• 2015

We investigated the effects of dietary lipid sources on growth and fatty acid composition of juvenile Chinese longsnout catfish. Triplicate groups of fish (initial average weight, 3.8 g) were fed four diets containing either fish oil (FO), soybean oil (SO), linseed oil (LO) and lauric acid (LA) for 10 weeks. There were no differences among the groups in body weight, feed intake, feed efficiency, protein efficiency ratio, and body proximate composition of fish fed the diets containing different lipid sources (P > 0.05) during the study. However, fatty acids compositions of the whole body were influenced by dietary lipid source. Fish fed the SO diet had high concentration of linoleic acid, whereas those of fish fed the LO diet were rich in linolenic acid and arachidonic acid. Fish fed the FO diet had significantly (P < 0.05) higher levels of monounsaturated fatty acids such as 18:1n-9 and 20:1n-9 than those of fish fed the SO and LO diets. Eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3) composition of body were not influenced by dietary lipid source. The results suggest that each of FO, SO, LO or LA can be used as a lipid source in the diets of Chinese longsnout catfish without any negative effects on growth and feed utilization and these data demonstrate the potential impact which dietary fat composition can change the body fatty acid profile.

• 한국식품영양과학회지
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• 제19권6호
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• pp.641-645
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• 1990

In order to study the influence of culture conditions on cell growth and lipid formation by Mucor sp, various carbon and nitrogen sources initial pH and C./N ratio of medium were investigated. Glucose was found to be suitable carbon source in terms of lipid yield and ${\gamma}$-linolenic acid(GLA) content. When NH4Cl and (NH4)2SO4 were used as nitrogen source lipid content was high(19-21%) but GLA content was low(15-17%) On the other hand when NaNO3 and KNO3 were used lipid content was low(about 13%) but GLA content was high(22-23%). The highest production of lipid was obtained at a C/N ratio of 40 using glucose and (NH4)2SO4 as carbon and nitrogen source respectively. it was found that lipid yield was high at pH4.6 Also this fungus did not grow at 35$^{\circ}C$ and lipid yield was highr at 15$^{\circ}C$ than $25^{\circ}C$.

• Journal of Korean Biological Nursing Science
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• 제19권2호
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• pp.107-112
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• 2017

Purpose: The purpose of this study was to identify the lipid profile ratios as factors affecting disease progress in patients with high-grade primary brain cancers undergoing concurrent chemoradiotherapy (CCRT) and adjuvant chemotherapy. Methods: The levels of lipid profile ratios were evaluated by looking at the total cholesterol (TC) to high-density lipoprotein (HDL)-cholesterol (TC/HDL-c), low-density lipoprotein (LDL)-cholesterol to HDL-cholesterol (LDL-c/HDL-c), and triglycerides to HDL-cholesterol (TG/HDL-c). This descriptive research was conducted 7 months after the initiation of CCRT and adjuvant chemotherapy. Results: A total of 36 patients with newly diagnosed primary malignant brain cancer were included in the study. The levels of lipid profile ratios such as TC/HDL-c, LDL-c/HDL-c, TG/HDL-c were significantly different between the patients with and without disease progress at 7 months after initiation of CCRT and adjuvant chemotherapy. Conclusion: The lipid profile ratios were indicators affecting disease prognosis with tumor-related factors at 7 months after initiation of CCRT and adjuvant chemotherapy. Therefore, lipid profile ratios indicating hyperlipidemia in patients with high-grade brain cancers should be carefully monitored during and after cancer-related therapy.