• Journal of Oriental Physiology
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• v.14 no.2 s.20
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• pp.1-9
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• 1999

Ethanol induces compoundhemorrhagic gastric lesions and causes a dose-dependent decrease in the concentration of endogenous nonprotein sulfhydryls in rat gastric mucosa. Sulfhydryl-containing drugs protect rats from ethanol - induced gastric lesions. Based on this findings, we investigated the involvement of sulfhydryl compounds in the antioxidant effects of Puerariae radix, a traditional herbal medicine, against ethanol - induced gastric lesions in the absence and presence of iodoacetamide(IDA. sulfhydryl blocking agent) in rats. respectively. Because of the known role of sulfhydryls in gastric cytoprotection, its role in gastric antioxidation was of intrest. In vitro, Puerariae radix extract(PRE) reduced linoleic acid autooxidation and exert DPPH radical scavenging effect. In vivo. PRE increased antioxidants(SOD, catalase. GSH) and reduced lipid peroxide level in ethanol-induced gastric mucosal lesions. But treatment with PRE plus IDA significantly inhibit the antioxidant effects such as SOD and GSH but did not affect catalase levels. These results suggest that Puerariae radix may play roles in the gastric cytoprotection through antioxidant effects and increase of SOD activity and GSH level are dependent of endogenous sulfhydryls.

• Applied Biological Chemistry
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• v.45 no.3
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• pp.168-172
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• 2002

Antioxidative activities of solvent fractions obtained from Korean Rhus vernicifera, being used in food industry and as a traditional medicine, were examined by the methods of DPPH scavenging activity, inhibitory effect on autooxidation of linoleic acid, nitrite scavenging activity and the inhibitory effect on xanthine oxidase activity. Ethyl acetate fraction showed potent antioxidative activities. Three compounds were isolated from the ethyl acetate fraction by rotatory locular counter current chromatography (RLCCC), Sephadex LH-20 column chromatography and HPLC. The isolated compounds were 1,2,3-trihydroxybezene, methyl 3,4,5-trihydroxybenzoate and 3,4,5-trihydroxybenzoic acid determined by GC/MS and, $^1H$ and $^{13}C$ NMR. Among the isolated compounds, methyl 3,4,5-trihydroxybenzoate showed the strongest antioxidative activity than artificial antioxidants, BHA and BHT in various methods. However, these isolated compounds did not show cytotoxicity effects on the human cancer cell lines. Therefore, we may suggest that methyl 3,4,5-trihydroxybenzoate can be used as a food additive possessing the potent antioxidative activity.

• Korean Journal of Medicinal Crop Science
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• v.15 no.3
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• pp.183-188
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• 2007

Efficacy of antioxidative activity and nitrite scavenging ability of methanol extract from Salvia plebeia was investigated. Electron-donating ability of extract at RC$_{50}$ was 51.10 ${\mu}$g/ml. After addition of 0.92 mg/ml extract, autooxidation of pyrogallol decreased to 62.11% by superoxide dismutase-like activity. In antioxidative activity of extract against linoleic acid during incubation times of 1,2, and 4 days at 40$^{\circ}$, lipid peroxidation values significantly decreased by 72.86%, 77.18%, and 78.32% with addition of 0.2 mg/ml, respectively. Total phenolic content was determined as gallic acid equivalents (GAE) and values revealed as 202.35${\pm}$ 1.26 GAE ${\mu}$g/ml of extract. Nitrite scavenging ability showed the most remark-able effect at pH 1.2, exhibited to 44.29% by addition of 0.2 mg/ml. These results suggest that methanol extract from Salvia plebeia can be used as bioactive and functional materials.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.3
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• pp.654-659
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• 2008

To confirm possibility of Nelumbo nucifera leaves as biofunctional material, we investigated the antioxidant activity and nitrite scavenging ability of ethanol extract from Nelumbo nucifera leaves. Nelumbo nucifera leaves were extracted with ethanol and concentrated under vacuum using rotary evaporator. Then, antioxidative activity and nitrite scavenging ability of the extract were examined in vitro. Electron -donating ability of the extract at RC50 was 90.19 ${\mu}g/mL$. After addition of 0.96 mg/mL, autooxidation of pyrogallol decreased to 66.19% by superoxide dismutase-like activity. In antioxidative activity of the extract against linoleic acid during incubation times of 24, 48, and 96 hours at $40^{\circ}C$, lipid peroxidation values significantly decreased to 72.53%, 82.00%, 84.69% with addition of 0.2 mg/mL, respectively. Total phenolic content was determined as gallic acid equivalents (GAE) and the value revealed to be $282.84\;{\pm}\;9.03$ GAE ${\mu}g/mg$ of the extract. Nitrite scavenging ability showed the most remarkable effect at pH 1.2, exhibited to 45.55% by addition of 0.2 mg/mL. These results suggest that ethanol extract from N. nucifera leaves can be used as bioactive and functional material.

• Journal of the Korean Society of Food Science and Nutrition
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• v.24 no.3
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• pp.409-414
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• 1995

Antioxidative characteristics of dihydroxyphenylalalnine(DOPA), melanin and enzymatic oxidation products of tyrosine(EOPTs) were studied in a model system. EOPTs were prepared by the tyrosine-tyrosinase reaction at pH 6.5 and $25^{\circ}C$ at various time intervals(0~120min). All EOPTs were brown in varied intensities with increased absorption at 200~210, 280, 310~320nm, and 450~490nm. EOPTs obtaiend at the early stage of the reaction(1~3min especially) showed a higher antioxidative activity than those from the later stage on the inhibition of peroxide, conjugated dienoic acid and malonaldehyde formations in linoleic acid autoxidation. Additionally among the substances of tyrosine, DOPA and melanin, DOPA showed the highest antioxidative activity while that of tyrosine was the lowest during the linoleic acid autooxidation. It was observed that DOPA and melanin had the ability of free radical scavenging, which may party contribute to their antioxidative activity.

• Korean Journal of Food Science and Technology
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• v.32 no.5
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• pp.1179-1185
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• 2000

Fermented anchovy was used to investigate its effects on the formation of lipid peroxide and the activities of epoxide or free radical generating enzyme in vitro in bromobenzene-treated rats. All solvent fractions from fermented anchovy not only showed the strong antioxidative activities on linoleic acid autooxidation, but also reduced bromobenzene-induced hepatic lipid peroxidation. The activities of aniline hydroxylase and aminopyrine N-demethylase elevated by bromobenzene were recovered to the level of normal rats by adding the solvent fractions of fermented anchovy. But, xanthine oxidase and aldehyde oxidase activities were not affected by fermented anchovy. These results suggest that reduction of the bromobenzene-induced hepatic lipid peroxidation is caused by inhibition on the epoxide formation, not on free radical generation.

• Journal of agriculture & life science
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• v.45 no.1
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• pp.109-117
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• 2011

The antioxidant activities of 60% ethanol extracts from different parts (leaf, stem, and root) of Artemisia argyi using an in vitro system were examined to find a possibility as natural antioxidant substances. The highest total phenolics (23.08 mg/g) was obtained by 60% ethanol extract of leaf. The scavenging activity of 60% ethanol extract from different parts of A. argyi on the DPPH and ABTS radical was raised with increasing amount of extracts, and 60% ethanol extract from leaf showed the highest radical scavenging activities. The leaf extract presented the highest reducing power and strong inhibitory effect on autooxidation of linoleic acid. In MDA formation using mouse brain homogenates, the 60% ethanol extracts of A. argyi also exhibited antioxidant activity as inhibition of MDA (71.38% at $200{\mu}g/mL$). Therefore, these results suggest that the 60% ethanol extract of A. argyi leaf possess excellent antioxidant activities and thus it has great potential as a source for natural antioxidant.