• Journal of Pharmaceutical Investigation
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• v.21 no.2
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• pp.67-71
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• 1991

A simple and sensitive method was developed for the quantification of free and conjugated bile acids in bezoar without prior hydrolysis. $3{\alpha}-Hydroxy$ bile acids are first oxidized to 3-keto bile acids in the reaction catalyzed by $3{\alpha}-hydroxysteroid$ $dehydrogenase(3{\alpha}-HSD)$. During this oxidative reaction, an equimolar quantity of nicotinamide adenine dinucleotide(NAD) is reduced to NADH and subsequently oxidized to NAD with concomitant reduction of nitrotetrazolium blue(NTB) to diformazan by the catalytic action of diaphorase. The diformazan has an absorbance maximum at 540 nm. The intensity of the color produced is directly proportional to bile acids concentration in the bezoar extracts. The optimum conditions for the enzymatic reaction such as effects of reaction time, reaction temperature and pH, and stability were investigated. Calibration plots for the sodium chelate observed to be linear and intra-, inter-assay analytical recovery of bile acids averaged $97.65{\pm}3.4%(S.D.)$. Therefore, it is considered that the quality control of total bile acids from bezoar or bezoar-containing liquid preparation using this simple and sensitive assay system will be acceptable. Also current bezoars and bezoar-containing liauid preparations were examined their total bile acids from this method.

• Environmental and Resource Economics Review
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• v.28 no.4
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• pp.497-526
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• 2019

The importance of "decoupling" to maintain economic growth and reduce greenhouse gases is emerging as the world has been mandated to reduce greenhouse gases since the 2015 Paris Agreement. This study covered 63 countries from 1980 to 2014 and analyzed the main characteristics and causes of decoupling phenomenon between economic growth and carbon emissions. In this study, the degree of decoupling was measured every five years. The analysis found that the decoupling rate of OECD countries and countries with large incomes was high, and that the decoupling phenomenon has accelerated worldwide since the 2000s. However, the degree of decoupling was different depending on the national characteristics. According to the results of dynamic panel model, the growth rate of manufacturing and the proportion of exports hampered decoupling, while the proportion of human capital and renewable energy had a positive effect on decoupling. Also income had a inverse U-shape non-linear effect on decoupling.

• Journal of Sensor Science and Technology
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• v.7 no.4
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• pp.279-284
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• 1998

A surface plasmon resonance (SPR) sensor system for the determination of sucrose concentration was constructed with a gold thin film sensing chip. The properties of gold thin film are critical factors in exciting surface plasmon resonance phenomena. Therefore in the present paper, the fabrication conditions of gold thin film were investigated to optimize the SPR phenomena. The optimum thickness was obtained as $545{\AA}$ with $43.75^{\circ}$ resonance angle and good surface roughness limitation. about $3{\AA}$. The linear resonance angle shifts and rapid response were observed from the sucrose concentrations ranged from 0 to 40wt%.

• Journal of Sensor Science and Technology
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• v.6 no.5
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• pp.356-361
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• 1997

Fiber-optic evanescent wave sensor was designed and fabricated to detect mouse immunoglobulin G(IgG) with decladed optical fiber on which anti-mouse IgG was immobilized. A sensitivity obtained by any direct or competitive method was lower than $1\;{\mu}g/m{\ell}$. Anti-mouse IgG was immobilized on 93.9% of core surface of optical fiber by simple adsorption method. The effect of postcoating using bovine serum albumin to remove non-specific binding was not observed. As the ratio of fluorescein to mouse IgG increased, the fluorescence signal increased, but that increase showed no linear relationship. Our fiber-optic sensor system could be used as immunosensor by measuring evanescent fluorescence in antigen-antibody reaction with good sensitivity below $1{\mu}g/m{\ell}$ level.

• Journal of Korean Society of Environmental Engineers
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• v.31 no.1
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• pp.64-69
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• 2009

Musty and earth odors caused by geosmin and 2-MIB are the major complaints from the drinking water consumers. Therefore, early detection of geosmin and 2-MIB is the key to prevent off-flavor occurrence. In this study, a rapid method using stir bar sorptive extraction (SBSE) in combination with the GC/MS was investigated to analyze geosmin and 2-MIB in water. The SBSE method, a solventless extraction technique, was optimized and then applied to the analysis of geosmin and 2-MIB in water. The SBSE technique was found to be a simple and fast procedure that allows many samples to be extracted simultaneously using very small volume (10~20 mL). In addition, the SBSE method offered high recovery and good linear regression coefficient for the geosmin and 2-MIB. The good repeatability of this method can be deduced from the low RSD (7.2~7.6%) at 10 ng/L for geosmin and 2-MIB. The limit of detection was determined 1~2 ng/L and the limit of quantitation was 3~6 ng/L. Above all, the SBSE method proved to be a very practical technique for the analysis of geosmin and 2-MIB in water.

• Journal of the Korean Society of Hazard Mitigation
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• v.8 no.2
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• pp.159-164
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• 2008

This study simulated the flood inundations of the Nakdong River catchment running through Yangsan, a small city located in the south eastern area of Korea by using the depth averaged two-dimensional hydrodynamic numerical model. The numerical model employs the staggered grid system including moving boundary and a finite different method to solve the Saint-Venant equations. A second order upwind scheme is used to discretize the nonlinear convection terms of the momentum equations, whereas linear terms are discretized by a second order Leap-frog scheme(Cho and Yoon, 1998). The numerical model was applied to a real topography to simulate the flood inundation of the Yangsan basin in Yangsan. The numerical result for urban district was visualization for three dimension. These results can be essentially utilized to construct the three dimensional inundation map after building the GIS-based database in local public organizations in order to protect the life and property safely.

• Journal of Korean society of Dental Hygiene
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• v.18 no.4
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• pp.489-499
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• 2018

Objectives: The purpose of this study was to investigate the mediating effects of burnout and to identify the moderating effects of individual factors and organizational factors on the relationship between emotional dissonance and job satisfaction. Methods: This cross-sectional study included 270 dental hygienists working full-time at dental care facilities. The data on the socio-demographic characteristics, emotional dissonance, burnout, job satisfaction, and individual and organizational factors were collected. The individual (self-efficacy and ego resilience) and organizational (social support, organizational support and wage satisfaction) factors were considered as the moderating variable. For statistical analyses, t-test, one-way ANOVA and multiple linear regression were used. Results: Burnout was found to be a significant mediator on the relationship between emotional dissonance and job satisfaction(p<0.001). The variables moderating the relationship between emotional dissonance and burnout were identified as social support, organizational support and wage satisfaction (p<0.05), while the variables moderating the relationship between burnout and job satisfaction were wage satisfaction and ego resilience (p<0.05). Conclusions: To prevent the decrease in job satisfaction due to emotional dissonance, the management of dental care facilities should have a better understanding of burnout in dental hygienists, which requires individual and organizational efforts to be moderated. In addition, as organizational support has been identified as the factor mitigating the negative effects of emotional dissonance, it is highly necessary to adopt the preceptor system, improve communication systems and expand welfare policies of organizations.

• Journal of Biosystems Engineering
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• v.38 no.1
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• pp.64-71
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• 2013

Purpose: The purpose of this study was to select standing posture parameters that have a significant difference according to the severity of spinal deformity, and to develop a novel Cobb angle prediction model for adolescent girls with idiopathic scoliosis. Methods: Five normal adolescents girls with no history of musculoskeletal disorders, 13 mild scoliosis patients (Cobb angle: $10^{\circ}-25^{\circ}$), and 14 severe scoliosis patients (Cobb angle: $25^{\circ}-50^{\circ}$) participated in this study. Six infrared cameras (VICON) were used to acquire data and 35 standing parameters of scoliosis patients were extracted from previous studies. Using the ANOVA and post-hoc test, parameters that had significant differences were extracted. In addition, these standing posture parameters were utilized to develop a Cobb-angle prediction model through multiple regression analysis. Results: Twenty two of the parameters showed differences between at least two of the three groups and these parameters were used to develop the multi-linear regression model. This model showed a good agreement ($R^2$ = 0.92) between the predicted and the measured Cobb angle. Also, a blind study was performed using 5 random datasets that had not been used in the model and the errors were approximately $3.2{\pm}1.8$. Conclusions: In this study, we demonstrated the possibility of clinically predicting the Cobb angle using a non-invasive technique. Also, monitoring changes in patients with a progressive disease, such as scoliosis, will make possible to have determine the appropriate treatment and rehabilitation strategies without the need for radiation exposure.

• Analytical Science and Technology
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• v.11 no.1
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• pp.47-53
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• 1998

This study is to improve the diagnosis of diabetes mellitus and the self-monitoring of blood glucose in people with diabetes by providing a non-invasive method of monitoring blood glucose. A near-infrared (NIR) spectrophotometer was used to measure absorption spectra of 80 glucose samples ranges from 1 mg/dL to 200 mg/dL, and shows the standard error of prediction 1.8 mg/dL. Also, to investigate the effect of interference in blood, NaCl and sand were added in glucose and found the standard error of prediction of 2.8 mg/dL and 3.8 mg/dL, respectively. A new and more accurate calibration system for the spectrophotometer was developed from systematic study of light scattering, which cause nonlinear spectrophotometer response.

• Analytical Science and Technology
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• v.18 no.6
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• pp.483-490
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• 2005

Mass resolution of the time of flight aerosol mass spectrometer for an aerosol component analysis depends on the initial direction and the initial energy of the ions. We have found that the shape of the optimum post focusing electric field is not linear. The maximum electric potential should be applied to the ions whose initial direction is 90 degree. To check on the post focusing effects, we have installed a laser ablation mass spectrometer. By using this LA-MS, we have found that the average energy distribution of the laser ablated ions is 8 eV. To establish the optimum mass resolution, a time delay and a high voltage are needed, and the results of the study show that 1500 nsec, and 3.7 kV are the optimum parameters for our system respectively. The isotope mass signals of copper show a good resolution.