• 대한전기학회:학술대회논문집
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• 대한전기학회 2006년도 추계학술대회 논문집 전기기기 및 에너지변환시스템부문
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• pp.125-128
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• 2006

This paper presents a novel type soft switching PWM power frequency AC-AC converter using bidirectional active switches or single phase utility frequency AC-high frequency AC matrix converter. This converter can directly convert utility frequency AC (UFAC, 50Hz/60Hz) power to high frequency AC (HFAC) power ranging more than 20kHz up to 100kHz. A novel soft switching PWM prototype of high frequency multi-resonant PWM controlled UFAC-HFAC matrix converter using antiparallel one-chip reverse blocking IGBTs manufactured by IXYS corp. is based on the soft switching resonance with asymmetrical duty cycle PWM strategy. This single phase UFAC-HFAC matrix converter has some remarkable features as electrolytic capacitor DC busline linkless topology, unity power factor correction and sine-wave line current shaping, simple configuration with minimum circuit components, high efficiency and downsizing. This series load resonant UFAC-HFAC matrix converter, incorporating bidirectional active power switches is developed and implemented for high efficiency consumer induction heated food cooking appliances in home uses and business-uses. Its operating performances as soft switching operating ranges and high frequency effective power regulation characteristics are illustrated and discussed on the basis of simulation and experimental results.

• Journal of Microbiology and Biotechnology
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• 제15권6호
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• pp.1258-1266
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• 2005

The present study was carried out in order to assess the protective effects of calycosin-7-O-$\beta$-D-glucopyranoside, isolated from Astragali radix (AR), on hyaluronidase (HAase) and the recombinant human interleukin-$1\beta$ (IL-$1\beta$)-induced matrix degradation in human articular cartilage and chondrocytes. We isolated the active component from the n-butanol soluble fraction of AR (ARBu) as the HAase inhibitor and structurally identified as calycosin-7-O-$\beta$-D-glucopyranoside by LC-MS, IR, ${1}^H$ NMR, and ${13}^C$ NMR analyses. The $IC_{50}$ of this component on HAase was found to be 3.7 mg/ml by in vitro agarose plate assay. The protective effect of ARBu on the matrix gene expression of immortalized chondrocyte cell line C28/I2 treated with HAase was investigated using a reverse transcription polymerase chain reaction (RT-PCR), and its effect on HAase and IL-$1\beta$-induced matrix degradation in human articular cartilage was determined by a staining method and calculating the amount of degraded glycosaminoglycan (GAG) from the cultured media. Pretreatment with calycosin-7-O-$\beta$-D-glucopyranoside effectively protected human chondrocytes and articular cartilage from matrix degradation. Therefore, calycosin-7-O-$\beta$-D-glucopyranoside from AR appears to be a potential natural ant-inflammatory or antii-osteoarthritis agent and can be effectively used to protect from proteoglycan (PG) degradation.

• BMB Reports
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• 제55권12호
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• pp.627-632
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• 2022

Dickkopf-1 (DKK1) is a secreted protein that acts as an antagonist of the canonical WNT/β-catenin pathway, which regulates osteoblast differentiation. However, the role of DKK1 on osteoblast differentiation has not yet been fully clarified. Here, we investigate the functional role of DKK1 on osteoblast differentiation. Primary osteoprogenitor cells were isolated from human spinal bone tissues. To examine the role of DKK1 in osteoblast differentiation, we manipulated the expression of DKK1, and the cells were differentiated into mature osteoblasts. DKK1 overexpression in osteoprogenitor cells promoted matrix mineralization of osteoblast differentiation but did not promote matrix maturation. DKK1 increased Ca⁺ influx and activation of the Ca⁺/calmodulin-dependent protein kinase II Alpha (CAMK2A)-cAMP response element-binding protein 1 (CREB1) and increased translocation of p-CREB1 into the nucleus. In contrast, stable DKK1 knockdown in human osteosarcoma cell line SaOS2 exhibited reduced nuclear translocation of p-CREB1 and matrix mineralization. Overall, we suggest that manipulating DKK1 regulates the matrix mineralization of osteoblasts by Ca⁺-CAMK2A-CREB1, and DKK1 is a crucial gene for bone mineralization of osteoblasts.

• 한국융합학회논문지
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• 제4권3호
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• pp.27-33
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• 2013

기존의 바코드는 정보 배열이 나란히 나열된 선 모양을 가지며 이를 1차원 바코드라 부른다. 이에 반해 2차원 바코드는 점자방식 또는 모자이크방식 코드로 작은 정사각형 도는 직사각형 안에 정보를 표현한다. 2차원 바코드는 기존의 1차원 바코드보다 작은 공간에 많은 데이터를 표현 가능함으로써 보다 효율적인 바코드의 구현이 가능하다. 현재 ISO 국제 표준화된 2차원 바코드는 총 4가지로 분류되는데 QR Code, Data Matrix, PDF417, MaxiCode가 있다. 본 논문에서는 ISO 국제 표준화된 바코드 중 하나인 Data Matrix의 Base 256 모드에 대한 기본 개념, 구성 방법, 인코딩 및 디코딩 방법을 상세히 제안한다. Data Matrix 심벌에 저장된 데이터를 보다 효율적으로 구성하기 위해 숫자, Alphanumeric 문자, 이진법에 따라 다른 인코딩, 디코딩 방법을 사용하게 되는데 본 논문에서는 이를 고려한 디코딩 방법에 초점을 맞춰 기술할 것이다.

• Bulletin of the Korean Chemical Society
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• 제33권3호
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• pp.790-794
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• 2012

Trace metal ions such as $Cd^{2+}$, $Ni^{2+}$, and $Zn^{2+}$ in a highly saline sample were subjected to on-line complexation with 4-(2-thiazolylazo) resorcinol (TAR) dissolved in a background electrolyte (BGE) under transient isotachophoresis (TITP) conditions. A long plug of the saline sample, containing the trace metal ions but devoid of TAR, was injected into a coated capillary filled with a BGE composed of 150 mM 2-(cyclohexylamino) ethanesulfonic acid (CHES) and 110 mM triethylamine (TEA) at pH 9.7. Since the electrophoretic mobility of TAR fell between the mobilities of the anionic leading electrolyte ($Cl^-$ in the sample) and the anionic terminating background electrolyte ($CHES^-$), a highly concentrated zone of TAR from the BGE was formed at the rear of the sample matrix and then the metal cations toward the cathode were swept by isotachophoretically assisted on-line complexation (IAOC) between the metal ions and the isotachophoretically stacked TAR. As a result, anionic metal-TAR complexes were formed efficiently, which satisfy the TITP conditions between $Cl^-$ and $CHES^-$. The enrichment factors of metal ions including $Cd^{2+}$ were up to 780-fold compared to a conventional CZE mode using absorbance detection. The detection limits were 17 nM, 15 nM, and 27 nM for $Ni^{2+}$, $Zn^{2+}$, and $Cd^{2+}$ in a 250 mM NaCl matrix, respectively. Our method was successfully applied to the analysis of urine samples without desalting.

• Journal of Mechanical Science and Technology
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• 제16권10호
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• pp.1187-1200
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• 2002

This paper is concerned with the design or an LMI (Linear Matrix Inequality) -based H$\infty$ controller for a line of sight (LOS) stabilization system and with its robustness performance. The linearization of the system is necessary to analyze various nonlinear characteristics, but the linearization entails modeling uncertainties which reduce its performance. In addition, the stability of the LOS can be adversely affected by angular velocity disturbances while the vehicle is moving. As the vehicle accelerates, all the factors that are Ignored and simplified for the linearization tend to Inhibit the performance of the system. The robustness in the face of these uncertainties needs to be assured. This paper employs H$\infty$ control theory to address these problems and the LMI method to provide a suitable controller with minimal constraints for the system. Even though the system matrix does not have a full rank, the proposed method makes it possible to design a H$\infty$ controller and to deal with R and S matrices for reducing the system order. It can be also shown that the proposed robust controller has a better disturbance attenuation and tracking performance. The LMI method is also used to enhance the applicability of the proposed reduced-order H$\infty$ controller for the system given. The LMI-based H$\infty$ controller has superior disturbance attenuation and reference input tracking performance, compared with that of the conventional controller under real disturbances.

• 건축역사연구
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• 제11권3호
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• pp.21-34
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• 2002

This study is to analyse the transformation process of spatial structure of traditional small city structure in Jeollabuk-Do in the process of modern urbanization between 19th century and the year 2000. The small cities, IM-PI, YONG-AN, YEO-SAN, OK-GOO, GO-SAN, MU-JANG, GUM-MA, GO-BU, which have been local administrative center in CHO-SUN dynasty, have role of local administrative center of subdivision of country-myon, except OK-GOO. The method of this study is to investigate the transformation process of spatial structural elements of with the actual field surveys, the analysis of Gunhyun map made in 1872, land registration maps in 1910s and 2000, and various topological maps. The elements of analysis are the topological site and geographical situation conditions, the urbanized areas, the street systems, the function of streets, the focal points of urban land use, the land use systems, and the location of major facilities such as administration facilities, markets, bus terminals. The analytical point of view and the results are as follows. There is strong relationship between the transformation of internal matrix route system and the growth pattern of urbanized areas, Especially on the contrary to the matrix route with East-West direction which has the role of checking the growth of urbanized area, the South-North direction route acts as the leading line of the growth. The focal points structure of urban land use shows transformation process from the point of central space of administrative district in front of Nae-A, to the access point to administrative district or to the access point to housing and neighborhood commercial area. From the point of functional area structure and major facilities location, line-type commercial area is developed along the line of central axis route and access route to administrative district. Especially direction of the growth of commercial area is strongly connected with the interrelationship between the study area and its neighboring cities. Pattern of commercial district development is varied with the direction of matrix route. That is, commercial district, which shows one side development pattern along the East-West direction route, is developed on both sides of street along the South-North direction route.

• 대한미생물학회지
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• 제35권1호
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• pp.9-18
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• 2000

Immunological mechanisms involving the release of inflammatory factors by HIV-1 infected microglia in the brain have been implicated in the pathogenesis of HIV dementia (HIVD). Since the regulation of matrix metalloproteinases (MMPs) activity can be influenced by variety of inflammatory mediators, this study was undertaken to look for a correlation between the MMP-9 release and the production of TNF-${\alpha}$ in response to HIV-1 p24 in the human monocyte cell line THP-1 as a model for microglia. First, it was shown that HIV-l core p24 antigen induced THP-1 to secrete MMP-9 in a dose response manner while it elicited a little effect on MMP-2 release in human astroglial cell line T98G. Next, it was found that p24 induced THP-1 to secrete TNF-${\alpha}$ without prior differentiation into macrophages by phorbol myristate acetate (PMA) treatment. Furthermore, anti-TNF-${\alpha}$ neutralizing antibodies significantly blocked p24-induced MMP-9 release in a dose dependent manner. Our data indicate that p24 antigen induces monocytic MMP-9 release by triggering up-regulation of TNF-${\alpha}$ secretion.

• 한국콘텐츠학회논문지
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• 제11권3호
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• pp.241-253
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• 2011

본 논문은 공간디자인 프로세스에서 on-line 기반 아이디어 발상 시스템인 웹스토밍(webstorming) 프로그램을 효과적으로 활용할 수 있는 디자인 프로세스 단계와 방법을 구체화하고 공간디자인에 특화된 아이디어 발상 기반지원시스템을 제안하고자 하였다. 이에 공간디자인 프로세스 단계에서의 활동요소와 웹스토밍 프로그램의 기능요소를 요소상관표로 작성하여 상관성 설문조사에 활용하였고, 요소상관표 작성결과를 시각화하여 관찰-해석-적용의 절차에 따라 분석하였다. 분석결과 공간디자인의 프로세스에서 긴밀한 협업이 이루어지는 계획설계 단계가 가장 높은 상관성을 가짐을 확인하였고, 분업화 작업시점을 기점으로 상관성에 차이가 발생함을 확인하였다. 이에 긴밀한 상관성을 갖는 계획설계 상의 작업에서 사용성을 높일 수 있는 방안으로 '세부 프로세스에 따른 발상매체 제공과 UI 최적화 등'을 제시하였고 이후 분업화 작업 시에도 웹스토밍 시스템의 활용성을 높일 수 있는 개선안을 제시하였다.

• 대한약침학회지
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• 제21권3호
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• pp.177-184
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• 2018

Objectives: Auraptene, a natural citrus coumarin, found in plants of Rutaceae and Apiaceae families. In this study, we investigated the effects of auraptene on tumor migration, invasion and matrix metalloproteinase (MMP)-2 and -9 enzymes activity. Methods: The effects of auraptene on the viability of A2780 and Hela cell lines was evaluated by MTT assay. Wound healing migration assay and Boyden chamber assay were determined the effect of auraptene on migration and cell invasion, respectively. MMP-2 and MMP-9 activities were analyzed by gelatin zymography assay. Results: Auraptene reduced A2780 cell viability. The results showed that auraptene inhibited in vitro migration and invasion of both cells. Furthermore, cell invasion ability suppressed at $100{\mu}M$ auraptene in Hela cells and at 25, $50{\mu}M$ in A2780 cell line. Gelatin zymography showed that for Hela cell line, auraptene suppressed MMP-2 enzymatic activity in all concentrations and for MMP-9 at a concentration between 12.5 to $100{\mu}M$ in A2780 cell line. Conclusion: Auraptene inhibited migration and invasion of human cervical and ovarian cancer cells in vitro by possibly inhibitory effects on MMP-2 and MMP-9 activity.