• Journal of Microbiology and Biotechnology
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• v.28 no.3
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• pp.367-374
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• 2018

RNA interference provides an effective tool for developing antitumor therapies. Cell-penetrating peptides (CPPs) are delivery vectors widely used to efficiently transport small-interfering RNA (siRNA) to intracellular targets. In this study, we investigated the efficacy of the cancer-specific CPP carrier BR2 to specifically transport siRNA to cancer-target cells. Our results showed that BR2 formed a complex with anti-vascular endothelial growth factor siRNA (siVEGF) that exhibited the appropriate size and surface charge for in vivo treatment. Additionally, the BR2-VEGF siRNA complex exhibited significant serum stability and high levels of gene-silencing effects in vitro. Moreover, the transfection efficiency of the complex into a cancer cell line was higher than that observed in non-cancer cell lines, resulting in downregulated intracellular VEGF levels in HeLa cells and comprehensively improved antitumor efficacy in the absence of significant toxicity. These results indicated that BR2 has significant potential for the safe, efficient, and specific delivery of siRNA for diverse applications.

• Journal of Environmental Impact Assessment
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• v.21 no.5
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• pp.631-648
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• 2012

The integrative ecological approaches of chemical assessments, physical habitat modelling, and multi-metric biological health modelling were applied to Gwanpyeong Stream within Gap-Stream watersheds to evaluate environmental impacts on the constructions of residential and commercial complex. For the analysis, the surveys conducted from 45 sites of reference streams within the Gap-Stream watershed and 3 regular sites during 2009 - 2010. Physical habitat health, based on the habitat model of Qualitative Habitat Evaluation Index(QHEI) declined from the headwaters(good - fair condition) to the downstream(poor condition). Chemical water quality, based turbidity and electric conductivity(EC), was degraded toward to the downstream, and especially showed abrupt increases, compared to the values of control streams(CS). Also, concentrations of chlorophyll-a in the downstreams were greater compared to the control stream(CS), indicating an eutrophication. Biological health conditions, based on the Index of Biological Integrity(IBI) using fish assemblages, averaged 19.3 which is judged as a fair condition by the biological criteria of the Ministry of Environment, Korea. The comparisons of model metric values in sensitive species and riffle-benthic species on the Maximum Species Richness Line(MSRL) of 45 reference streams indicated a massive disturbances in all sampling locations. Also, tolerance guild and trophic guild analyses suggest that dominances of tolerant species and omnivores were evident, indicating a biological degradation by habitat disturbances and organic matter pollutions. There was no distinct longitudinal variations of IBI model values from the headwater to the downstream in spite of slight chemical and habitat health gradients among the sampling sites. Overall, integrative ecological health(IEH) scores, based on the chemical, physical, and biological parameters, were low compared to the 45 reference streams due to physical and chemical disturbances of massive constructions of the residential and commercial complex. This stream, thus showed a tendency of typical urban streams which are disturbed in the chemical water quality, habitat structures, and biological integrity. Effective stream management plans and restoration strategies are required in this urban stream for improving integrative stream health.

• 한국태양에너지학회:학술대회논문집
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• 2008.11a
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• pp.245-250
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• 2008

This study is on the availability of solar thermal energy in Korean high-rise apartment complex depending on the installation type of solar collectors to roof or facade of building. Firstly, solar access evaluation on the roof and the facade of apartment buildings was carried out. The total thermal load of each apartment unit and building was investigated and matched with the energy which was produced by solar thermal systems on the facade. The considered layout patterns of apartment buildings were '一type', 'alternative 一type', 'ㄱtype' and 'ㅁtype' and that was analyzed in prior studies. Extensive dynamic hourly energy simulations with the solar thermal system were Performed with the TRNSYS of SEL. We assumed that the apartment complex is composed of 9 buildings and located in Daejeon. The collectors are the heat-pip evacuated tube collectors and the number of collectors are 45 tubes We assumed that the collectors are installed on the balcony of each unit and the angle of incilnation is $90^{\circ}$. As a result, the supply amount of solar thermal systems is about 4,850,086kJ/hr and the solar fraction is about 66%. The solar fraction according to each azimuth is about 66% on the south, 62% on the south-east $30^{\circ}$ and 56% on the south-east $60^{\circ}$. So, we quantitatively got a line on the optimal azimuth for installing the solar thermal systems. The solar fraction has differences from 5% to 15% of each floor, 6th, 12th and 20th and those tendencies are same in analyzed each 4 types of the apartment complexes.

• The Korean Journal of Pain
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• v.19 no.2
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• pp.223-227
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• 2006

Complex regional pain syndrome (CRPS) is clinically characterized by pain, abnormal regulation of blood flow and sweating, edema of skin and subcutaneous tissues, sensory and motor disturbances, and trophic changes of the skin. A 21-year-old man was suffering from pain and swelling in his right hand and forearm. His arm had been in splints for 3 weeks following an extension injury of the right fingers and wrist, with the pain having developed 2 weeks after the splinting. He was treated with various nerve blocks including continuous epidural infusion, thoracic sympathetic block and peripheral nerve blocks, and squeezing his edematous region under general anesthesia as well as intravenous lidocaine and ketamine infusions. However, all of the performed treatments had no effect on the patient's pain or hand swelling. As a next line therapy, spinal cord stimulation should be considered because of intractable severe pain and swelling to almost all other modalities of therapy. We therefore performed an early intervention of spinal cord stimulation for the patient with refractory CRPS type I 5 months after the onset of pain and have got an excellent result.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.21 no.5
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• pp.380-390
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• 2009

This study was conducted as a part of investigating pre-reclamation shorelines from aerial photographs to estimate coastal land area at reclaimed lands (Anjeong industrial complex, Myeongji residental complex, and Noksan industrial complex), southeastern coast of Korea. To assess how the shorelines were suitable for the calculation of coastal lands, we constructed shoreline change data. Secondary ground control points were used to accomplish triangulation for old aerial photographs. Two kinds of shorelines were mapped; one was the shoreline based on approximately highest high water level (AHHWL) and the other was the high water line based on wet/dry signiture. These shorelines were consistent at artificial coast. Shoreline change data were built with a variety of levels of error due to detailed differences in the photograph scale, quality of image, type of ground control point and type of shoreline. Thus assessment of the pre-reclamation shorelines at the level of qualitative analysis for the trend of shoreline changes was satisfactory. Most of shoreline changes before reclamation in this study were associated with coastal development. Investigation of shoreline attributes in relation to aerial photographs allowed us to understand the shoreline changes.

• Journal of the Korean Institute of Intelligent Systems
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• v.18 no.6
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• pp.797-807
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• 2008

This paper proposes a new algorithm of extracting forward vehicle areas using the acquired lanes and road area information on road images with complex background to improve the efficiency of the vehicle detection. In the first stage, lanes are detected by taking into account the connectivity among the edges which are determined from a method of chain code. Once the lanes proceeding to the same direction with the running vehicle are detected, neighborhood roadways are found from the width and vanishing point of the acquired roadway of the running vehicle. And finally, vehicle areas, where forward vehicles are located on the road area including the center and neighborhood roadways, are extracted. Therefore, the proposed scheme of extracting forward vehicle area improves the rate of vehicle detection on the road images with complex background, and is highly efficient because of detecting vehicles within the confines of the acquired vehicle area. The superiority of the proposed algorithm is verified from experiments of the vehicle detection on road images with complex background.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.39 no.11
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• pp.1153-1159
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• 2015

Recently, synthetic biopolymers and bioceramics such as poly (${\varepsilon}$-caprolactone)(PCL), hydroxyapatite, tricalcium phosphate, biphasic calcium phosphate(BCP), and zirconia have been used as substrates to generate various tissues or organs in tissue engineering. Thus, the purpose of this study was the characterization of $ZrO_2$/BCP/PCL(ZBP) scaffold for bone tissue regeneration. Based on the result of single-line test, blended 3D ZBP scaffolds with fully interconnected pores and new complex pore pattern of $45^{\circ}+135^{\circ}$-type and staggered-type were successfully fabricated using a polymer deposition system. Furthermore, the effect of ZBP scaffold on mechanical property was analyzed. In addition, in vitro cell interaction of ZBP scaffold on MG63 cells was evaluated using a cell counting kit-8(CCK-8) assay.

• Corrosion Science and Technology
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• v.7 no.4
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• pp.208-211
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• 2008

Safety operation of main pipelines is primarily provided by anticorrosive monitoring. Anticorrosive monitoring of oil pipeline transportation objects is based on results of complex corrosion inspections, analysis of basic data including design data, definition of a corrosion residual rate and diagnostic of general equipment's technical condition. All the abovementioned arrangements are regulated by normative documents. For diagnostics of corrosion-technical condition of oil pipeline transportation objects one presently uses different methods such as in-line inspection using devices with ultrasonic, magnetic or another detector, acoustic-emission diagnostics, electrometric survey, general external corrosion diagnostics and cameral processing of obtained data. Results of a complex of diagnostics give a possibility: $\cdot$ to arrange a pipeline's sectors according to a degree of corrosion danger; $\cdot$ to check up true condition of pipeline's metal; $\cdot$ to estimate technical condition and working ability of a system of anticorrosive protection. However such a control of corrosion technical condition of a main pipeline creates the appearance of estimation of a true degree of protection of an object if values of protective potential with resistive component are taken into consideration only. So in addition to corrosive technical diagnostics one must define a true residual corrosion rate taking into account protective action of electrochemical protection and true protection of a pipeline one must at times. Realized anticorrosive monitoring enables to take a reasonable decision about further operation of objects according to objects' residual life, variation of operation parameters, repair and dismantlement of objects.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.10a
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• pp.354-361
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• 2005

In high speed cutting process, due to the friction between the tool and workpiece, a temperature rise of contacting part is serious. It need to develop cutting tool for overcoming such a poor condition. So now, some studies, the optimization of tool shapes, the fine grains of tool material, multi-layer coating of tools are processing. If mirror finishing on the tool is processed, there is advantage of relation between chip and tool, because of less friction, and also tool's lift would be increased. As a result mirror like finishing is expected efficient enhancement of tool. Generally, it is too difficult to process by a general way for tools of complex shapes, it is required a new method to process such complex shape tools. The magnetic fluid polishing technique can polish the workpiece of complex shape, because the polishing method which polishes as compress the workpiece by the magnetism abrasives to arrange to the linear according to the line of magnetic force. In this paper, We polished the surface of the high speed cutting tool using the magnetic fluid polishing technique, to enhance the performance of the high speed cutting tool.

• Bulletin of the Korean Chemical Society
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• v.14 no.5
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• pp.561-567
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• 1993

The twelve macrocycle (L) complexes of cadmium(II) nitrate have been synthesized: $CdL(NO_3)_2$. All the complexes have been indentified by elemental analysis, electric conductivity measurements, IR and NMR spectroscopic techniques. The molar electric conductivities of the complexes in water and acetonitrile solvent were in the range of 236.8-296.1 $cm^2{\cdot}mol^{-1}{\cdot}ohm^{-1}$ at 25$^{\circ}$C. The characteristic peaks of macrocycles affected from Cd(II) were shifted to lower frequencies as compared with uncomplexed macrocycles. A complex with 1,4,8,11-tetrakis(methylacetato)-1,4,8,11-tetraaza cyclodecane (L4) exhibited two characteristic bands such as strong stretching (1646 $cm^{-1})$, and weaker symmetric stretching band (1384 $cm^{-1})$. NMR studies indicated that all nitrogen donor atoms of macrocycles have greater affinity to cadmium(II) metal ion than do the oxygen atoms. The $^{13}$C-resonance lines of methylene groups neighboring the donor atom such as N and S were shifted to a direction of high magnetic field and the order of chemical shifts were $L_1 < L_2 < L_3 < L_6 < L_4$. Also the chemical shifts values were larger than those of methylene groups bridgeheaded in side-armed groups. This result seems due to not only the strong interaction of Cd(Ⅱ) with nitrogen donors according to the HSAB theory, but weak interaction of Cd(Ⅱ) and COO- ions or sulfur which is enhanced by the flexible methylene spacing group in side-armed groups. Thus, each additional gem-methyl pairs of L_3, L_4\;and\; L_6$ macrocycles relative to $L_1, L_2,\;and\;L_5$ leads to an large enhancement in Cd(II) affinity. ^{13}C$-NMR spectrum of the complex with $L_{12}$ (1,5,9,13-tetracyclothiacyclohexadecane-3,11-diol) reveals the presence of two sets of three resonance lines, and intensities of the each resonance line have the ratio of 1 : 2 : 2. This molecular conformation is predicted as structure of tetragonal complex to be formed by coordinating two sulfur atoms and the other two sulfur atoms which is affected by OH-groups.