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Isolation and structural elucidation of the herbicidal active compounds from Ligularia stenocephala M.

  • Lim, Chi-Hwan;Cho, Chong-Woon
    • Korean Journal of Agricultural Science
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    • v.48 no.2
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    • pp.343-351
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    • 2021
  • Screening was conducted using 200 kinds of plant extracts to explore herbicide-activated components of plant origin. We separated and purified active substances and elucidated chemical structures using Ligularia stenocephala M., which has strong activity and has not yet been studied. When the solvent fractions of the leaves of Ligularia stenocephala M. were tested for their herbicidal activity, ethyl acetate and chloroform layer showed an inhibition rate of 95.2% and 94.1%, respectively. In particular, the chloroform layer exerted more than 50% herbicidal activity at 10 ppm. From the chloroform layer with the highest herbicidal activity, we isolated three herbicidal active compounds using stepwise chromatography, specifically silica gel or octadecyl silica (ODS) column chromatography, Sep-pak cartridges, and high performance liquid chromatography (HPLC). Based on the analysis of the active compounds using electron ionization mass spectroscopy (EI-MS), 1H-NMR, and 13C-NMR, we identified the active compounds as euparin, 5,6-dimethoxy-2-isopropenylbenzofuran, and liguhodgsonal. When the herbicidal activity of the identified compounds was tested, euparin showed selective herbicidal activity for lettuce at 10-3 M, and both liguhogsonal and 5,6-dimethoxy-2-isoprophenylbenzofuran exerted selective activity for rice and Echinochloa crus-galli.

Fabrication of a Full-Scale Pilot Model of a Cost-Effective Sodium Nickel-Iron Chloride Battery Over 40 Ah

  • Lee, Dong-Geun;Ahn, Byeong-Min;Ahn, Cheol-Woo;Choi, Joon-Hwan;Lee, Dae-Han;Lim, Sung-Ki
    • Journal of Electrochemical Science and Technology
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    • v.12 no.4
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    • pp.398-405
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    • 2021
  • To fabricate a full-scale pilot model of the cost-effective Na-(Ni,Fe)Cl2 cell, a Na-beta-alumina solid electrolyte (BASE) was developed by applying a one-step synthesis cum sintering process as an alternative to the conventional solid-state reaction process. Also, Fe metal powder, which is cheaper than Ni, was mixed with Ni metal powder, and was used for cathode material to reduce the cost of raw material. As a result, we then developed a prototype Na-(Ni,Fe)Cl2 cell. Consequently, the Ni content in the Na-(Ni,Fe)Cl2 cell is decreased to approximately (20 to 50) wt.%. The #1 prototype cell (dimensions: 34 mm × 34 mm × 235 mm) showed a cell capacity of 15.9 Ah, and 160.3 mAh g-1 (per the Ni-Fe composite), while the #2 prototype cell (dimensions: 50 mm × 50 mm × 335 mm) showed a cell capacity of 49.4 Ah, and 153.2 mAh g-1 at the 2nd cycle.

Cytosolic domain regulates the calcium sensitivity and surface expression of BEST1 channels in the HEK293 cells

  • Kwon Woo Kim;Junmo Hwang;Dong-Hyun Kim;Hyungju Park;Hyun-Ho Lim
    • BMB Reports
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    • v.56 no.3
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    • pp.172-177
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    • 2023
  • BEST family is a class of Ca2+-activated Cl- channels evolutionary well conserved from bacteria to human. The human BEST paralogs (BEST1-BEST4) share significant amino acid sequence homology in the N-terminal region, which forms the transmembrane helicases and contains the direct calcium-binding site, Ca2+-clasp. But the cytosolic C-terminal region is less conserved in the paralogs. Interestingly, this domain-specific sequence conservation is also found in the BEST1 orthologs. However, the functional role of the C-terminal region in the BEST channels is still poorly understood. Thus, we aimed to understand the functional role of the C-terminal region in the human and mouse BEST1 channels by using electrophysiological recordings. We found that the calcium-dependent activation of BEST1 channels can be modulated by the C-terminal region. The C-terminal deletion hBEST1 reduced the Ca2+-dependent current activation and the hBEST1-mBEST1 chimera showed a significantly reduced calcium sensitivity to hBEST1 in the HEK293 cells. And the C-terminal domain could regulate cellular expression and plasma membrane targeting of BEST1 channels. Our results can provide a basis for understanding the C-terminal roles in the structure-function of BEST family proteins.

Understanding the seasonal status of fisheries resource in the East China Sea by using bottom trawl (저층트롤을 이용한 동중국해 해역의 계절별 수산자원 현황)

  • Hyoung-Ho SHIN;Jungkwan LEE;Geunchang PARK;Byeong Gwon LIM;Doo Jin HWANG;Kyounghoon LEE
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.59 no.3
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    • pp.231-241
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    • 2023
  • This study was investigated in order to find composition and density of fisheries resource using bottom trawl in April, July, August, and November 2022 in the East China Sea. The average density of fisheries resource was estimated using the swept area method. As a result, 35 species were collected from the East China Sea. These included 21 fishes, six crustaceans, six cephalopods and two echinoderms. Seasonally, the average density of crustacean individuals per unit area were highest in November (692.1 inds./km2), while cephalopod individuals per unit area were highest in August (39.4 inds./km2). The average density of fish individuals per unit area were highest in August at 355.0 (inds./km2).

Assessment of GCM and Scenario Uncertainties under Future Climate Change Conditions

  • Jang, S.;Hwang, M.;Park, J.;Lim, K.
    • International conference on construction engineering and project management
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    • 2015.10a
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    • pp.658-659
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    • 2015
  • GCM and scenario uncertainties are first investigated for 5 major watersheds (Han River, Paldang dam, Namhan River, Bukhan River and Imjin River watersheds). As a result of this study, it is found that CCSM3-based annual precipitation increases linearly with respect to the 10-year moving average values while CSIRO-based precipitation does not show much of trend. The results from annual DJF mean precipitation show a similar trend with respect to their 10-year moving average values. Both CCSM3- and CSIRO-based annual JJA mean precipitation do not show much of trend toward 21st century. In general, CCSM3-based precipitation values are slightly higher than CSIRO-based values with respect to their annual and annual JJA mean precipitation values, but CSIRO-based annual DJF mean precipitation values are slightly higher than CCSM3-based values. In case of mean air temperature between CCSM3 and CSIRO during 21st century, all of results show a clear trend in warming with the passage of time for 5 watersheds. However the upward trends from CCSM3-based values slow down toward end of 21stcentury while CSRIO-based values increases almost linearly.

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Analysis of Flood Reduction in Downstream Urban Areas for the Storage in Apartment Complex (하류 도심지 침수저감 분석을 통한 공동주택 단지의 우수저류조 계획)

  • Choi, Jae-Do;Lim, Hyoung-Chul
    • Proceedings of the Korean Society of Disaster Information Conference
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    • 2023.11a
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    • pp.301-302
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    • 2023
  • 연구목적:본 논문에서는 기후변화로 기존 도심지 침수 발생이 증가하는 현 상황에서 공동주택 단지에 소규모 우수저류시설을 계획하였을 때 기존 하류부 도심지 침수량 증가율과 기존 도심지 침수량 저감 정도를 분석하고자 한다. 연구방법:미국환경보호청 SWMM 모델을 활용해서 대규모 하수도 모델을 구축하고 시뮬레이션을 통해 하류부 기존 도심지 침수영향을 분석하였다. 구축한 모델은 개발지구, 하류부 기존 도심지, 우수를 방류하는 하천유역 전체를 포함하였다. 연구결과: 대규모 개발행위 시 연구대상지역에서 공동주택 블록별로 최소 우수저류조 용량을 산정하여 시뮬레이션한 결과, 하류부 기존 도심지에서 방재성능목표강우량 1시간 기준 4,893m3, 2시간 기준 25,815m3, 3시간 기준 55,528m3의 침수량을 저감할 수 있을 것으로 나타났다. 결론:본 연구와 같이 하류부 기존 도심지까지 고려한 대규모 침수 시뮬레이션을 실시하였을 때 하류부 도심지 침수가 크게 증가하는 것을 알 수 있고, 개발전 공동주택 단지 블록별로 필요한 우수저류조 용량을 계획하고 개발시 비용 지원 등으로 우수저류조 건설을 권장한다면 하류부 기존 도심지 홍수 부담을 감소시킬 수 있다.

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Microfiber-based Textile Pressure Sensor with High Sensitivity and Skin-breathability (높은 민감도 및 우수한 피부 통기성을 가진 마이크로 섬유 기반의 직물형 유연 압력 센서)

  • Kangto Han;Jang-hee Choi;Jeongwoo Lim;Hyeyoung Gong;Geun Yeol Bae
    • Textile Coloration and Finishing
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    • v.35 no.3
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    • pp.179-187
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    • 2023
  • In this study, we developed a microfiber-based flexible pressure sensor with high sensitivity and excellent skin breathability. A nonwoven fabric composed of microfibers was prepared by electrospinning, which resulted in excellent moisture permeability of the sensor (143 g∙m-2∙h-1). In particular, high-pressure sensitivity (0.36 kPa-1) was achieved by introducing submicron structures on the microfiber surface by controlling the ambient humidity during electrospinning. The fabrication technology of the microfiber-based flexible pressure sensors reported in this study is expected to contribute to the commercialization of flexible pressure sensors applicable to long-term wearable health monitoring as well as virtual/augmented reality and electronic skin applications.

Isolation and Identification of Three Newly Reported Ascomycete Fungal Species Isolated from Soil in Korea

  • Mohammad Hamizan Azmi;Seong-Keun Lim;Seok-Jin Park;Min-Gyeong Song;Jun-Soo Cha;Seung-Yeol Lee;Hee-Young Jung
    • The Korean Journal of Mycology
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    • v.52 no.2
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    • pp.97-108
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    • 2024
  • In this study, three fungal isolates belonging to the phylum Ascomycota under classes Leotiomycetes, Eurotiomycetes, and Sordariomycetes were isolated from soil in Korea. These species were designated as KNUF-22-003, KNUF-22-005, and KNUF-20-NI016, respectively, and identified based on their phylogenetic relationships and morphological characteristics. The isolates were confirmed through molecular phylogenetic analyses of their internal transcribed spacer (ITS) region, 28S rDNA large subunit (LSU), and actin (ACT1 ) gene sequences. Cultural and morphological characteristics of strains KNUF-22-003, KNUF-22-005, and KNUF-20-NI016 were matched with Chaetomella oblonga CBS110.78T, Oidiodendron chlamydosporicum CBS403.69T, and Sarocladium subulatum CBS217.35T, respectively. To the best of our knowledge, this is the first report on C. oblonga, O. chlamydosporicum, and S. subulatum in Korea.