• 한국연초학회지
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• 제16권1호
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• pp.20-25
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• 1994

The volatile components were extracted from root of Cnidium officinale M. by SDE(Simultaneous steam distillation and extraction) apparatus and analyzed by GC/M.5 and GC retention index matching. The experimental results revealed the presence of over 22 volatile components. Major components were cnidilide (35.1%), neocnidilids (13.4%), ligustilide (23.2%). The essential oils were separated by silica gel column chromatography(Merck 70-230mesh), and 4 fractions among 12 fractions separated had a, good aroma character.

• 대한한의학회지
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• 제18권1호
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• pp.187-197
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• 1997

Major separation for the active ingredients and characterization of chemical properties in conjunction with screening test on animal were performed in order to analyze and standardize Ligustici Rhizoma or Angelicae Tenuissimae Radix as an important oriental herbal medicine for antiphlogistic or an important oriental herbal medicine for antiphlogistic or an anodyne. Furthermore the structure, composition and contents of ingredients for essential oil in Angelicae Tenuissimae Radix(Suckpo, Korea) were determined by means of Ge/MS followed by screening test on Z-ligustilide(82%) known as major ingredient as well as butylidenephthalide collected by HPLC with normal phase semiprep-column. The total active ingredient in Ligustici Rhizoma from China or Angelicae Tenuissimae harvested at Choonyang(Kyungnam, Korea), Jungsun(Kangwon, Korea), Suckpo(Kyungnam Korea), Youngchun(Kyungnam, Korea) have been determined showing higher abundant for three times on the product in Korea compared to that in China. In addition, the major component in Ahgelicae Tebyussunae Radux extract was found to be Z-ligustilide(70-80%) which is very different from that in Ligustici Rhizoma senkyunolide(39%) as major species. For screening test of Ligustici Rhizoma or Angelicae Tenuissimae Radix extracts toward the target animal, the efficiency has been shown the similarity on both extracts. Taking into account the level of ingredient, the total efficiency may be three times higher on Angelicae Tenuissimae Radix in Korea compared to Ligustici Rhizoma in China. As a result of present study, it is preferable to distinguish between Ligustici Rhizoma and Angelicae Tenuissimae Radix for better usage of oriental herbal medicine because of very different composition and abundant in spite of their similar screening effect.

• 대한화장품학회지
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• 제16권1호
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• pp.30-39
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• 1990

Angelica gigas extract and Cnidium of ficinale extract in Bath essence were determined by high performance liquid chromatography with a UV detector by use of Bondapak C-18 column. Decursin and Ligustilide were used as indicator ingredient for analysis of Angelica gigas extract and Cnidium of ficinale extract. Each indicator ingredient was isolated from each plant and purified using silver-ni-trate treated silica column. Their structure were conformed with IR, NMR and HPLC. The recoveries of Angelica gigas extract and Cnidium officinale extract added to Bath essence sample were 96.5-109.5% and 112-115%.

• 한국자원식물학회지
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• 제33권5호
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• pp.419-427
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• 2020

토천궁(L. chuanxiong Hort.)은 산형과(Umbelliferae) 기름 당귀속(Ligusticum)에 속하는 다년생 식물로, 뿌리를 이용하는 효능 및 약리작용으로 인하여 약용 목적으로 재배된다. 입지환경과 생육 특성 및 지표성분과의 상관관계를 파악한다면 보다 명확한 토천궁의 임간 재배환경 조건을 확립할 수 있을 것이며, 사회·경제적으로 관심이 증가하고 있는 고품질의 단기소득임산물 생산을 위한 기초자료를 확보할 수 있을 것으로 사료된다. 따라서 본 연구의 목적은 기후변화에 대응하여 토천궁의 기후대별 입지환경과 생육 특성 및 성분 함량과의 상관관계를 구명하여 최적의 재배환경에 대한 정보를 제공하는데 있다. 기후대별 입지환경 차이에 따른 토천궁의 특성을 비교하기 위해 온대 남부의 경상남도 함양군 삼봉산, 온대중부의 경상북도 봉화군 미림산, 온대북부의 강원도 정선군 가리왕산에 각 시험지를 선정하였고, 토천궁을 이식해 생육 특성, 토양 이화학성 분석, 기후 측정, 지표성분 분석을 실시하였다. 그 결과, 정선의 토천궁 생중량 및 건중량이 49.08 g 및 20.89 g으로 가장 높았으며, 근장 및 근직경도 각각 22.79 cm 및 52.46 mm로 함양과 봉화보다 높았다. 유효성분 함량은 정선에서 senkynolid A, z-ligustilide, chlorogenic acid, trans-ferulic acid 총 함량이 21.59 mg/g으로 높게 나타났다. 토천궁의 수량과 성분특성은 생육 환경에 영향을 받으며, 해발고가 높고 기온이 낮으며 토양 수분함량이 적은 지역에서 재배하는 것이 유효성분 함량과 생산량 증가에 유리하다고 사료된다. 본 연구는 환경조건과 생육 특성 및 지표성분 함량과의 상관관계에 대한 자료로써 산림 내 재배환경의 확립 및 연구의 기초가 될 것으로 판단된다.

• Journal of Applied Biological Chemistry
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• 제63권1호
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• pp.29-33
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• 2020

한약재의 불법 유통을 방지하기 위해 신속 정확한 원산지 판별방법 개발이 필요함에 따라 TDU-GC/MS를 이용하여 한국품종 당귀와 외국품종 당귀를 판별함과 동시에 한국품종 당귀의 재배지가 한국인지를 판별할 수 있는 기법을 연구하였다. 당귀추출물을 열탈착 시킨 후 냉각응축시스템에서 응축시켜 일시에 GC/MS로 분석한 결과 국산 품종 당귀(참당귀)는 TIC의 RT 26.9-27.2에서 coumarin 유도체인 decursin과 decursinol peak가 확인되었다. 중국 품종 당귀(중당귀)의 경우 RT 17.2 부근에서 ligustilide의 peak가 검출되었다. 국산 품종 참당귀 원산지에 따른 휘발성분의 차이를 알아보기 위하여 twister로 흡착하여 TDU-GC/MS로 m/z 40-400 amu 범위에서 mass spectrum을 측정하였다. 참당귀의 국내 및 중국 재배 시료 TIC는 전반적으로 같은 경향을 냈으나 TIC를 부분 scan한 결과 RT 15.4-16.1에서 국내 및 중국 재배의 peak pattern 차이를 확인할 수 있었다. Peak A (RT 15.54)과 B (RT 16.05)의 비율은 국내 재배는 0.0-0.2, 중국 재배의 경우 0.5-2.8으로서 TDU-GC/MS의 TIC peak pattern 비교를 통한 원산지 판별 가능성을 확인하였다.

• 한국자원식물학회지
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• 제31권4호
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• pp.312-321
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• 2018

Angelica tenuissima, also known as Ligusticum tenuissimum, is classified as a food-related plant and has been used as traditional medicines treating headache and anemia in Asia. However, its anti-melanogenic effect has not been reported in detail. When the extract of Angelica tenuissima (ATE) was prepared by the extraction with 70% EtOH at $80^{\circ}C$ (final yield = 22%), the contents of decursin and Z-ligustilide in ATE were determined 0.06% and 8.43%, respectively. Total flavonoid and phenolic content in mg ATE were $5.52{\pm}0.07{\mu}g$ quercetin equivalents and $237.27{\pm}13.24{\mu}g$ gallic acid equivalents, respectively. Antioxidant capacity of ATE determined by DPPH and ABTS assay was increased with a dose dependent manner up to $1000{\mu}g/m{\ell}$. The amount of melanin synthesis followed by ${\alpha}-melanocyte$ stimulating hormone on B16F10 cells were significantly reduced in the presence of ATE (250 to $1000{\mu}g/m{\ell}$, p<0.05). ATE (125 to $1000{\mu}g/m{\ell}$, p<0.05) suppressed the tyrosinase activity but did not show any significant effect on ${\alpha}-glucosidase$ activity at the same condition. Taken together, ATE possesses tyrosinase inhibitory potential with significant antioxidant capacities. These effects of ATE might be involved in suppression of melanin synthesis, at least, in B16F10 cells. The anti-melanogenic potential of ATE will provide an insight into developing a new skin whitening product.

• 한국식품영양학회지
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• 제30권2호
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• pp.363-370
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• 2017

This study investigated the chemical composition of headspace gas from white-flowered lotus (Nelumbo nucifera Gaertner). Volatile flavor compositions of headspace from white-flowered lotus (floral leaf, stamen, flower stalk, stem) were investigated through the solid-phase microextraction method using polydimethylsiloxane-divinylbenzene fiber. The headspace was directly transferred to a gas chromatography-mass spectrometry. Sixty-three volatile flavor constituents were detected in the headspace of lotus floral leaves, and undecanoic acid (7.81%) was the most abundant component. Fifty-three volatile flavor constituents were detected in the headspace of lotus stamina, and isobutylidene phthalide (7.94%) was the most abundant component. Forty-four volatile flavor constituents were detected in the headspace of lotus flower stalks, and 3-butyl dihydrophthalide (11.23%) was the most abundant component. Fifty-nine volatile flavor constituents were detected in the headspace of lotus stems, and ligustilide (16.15%) was the most abundant component. The content of phthalides was higher in the headspace of flower stalks and stems, while alcohols and acids were the predominant compounds in lotus floral leaves.

• Natural Product Sciences
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• 제18권4호
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• pp.244-249
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• 2012

Angelica acutiloba is one of the most intensively cultivated medicinal plants in Korea. The roots of this plant have been used as an important herbal drug, especially for the treatment of various female disorders, as the traditional therapy in Korea and other Asian countries. Consumption of its fresh leaves as a healthy vegetable has recently increased. In this study, essential oil fractions were extracted from the roots and leaves of this plant by steam distillation. Compositions of the two oils were compared by gas chromatography-mass spectrometry (GC-MS). The antibacterial activities of the essential oil were determined against three strains of Escherichia coli. DPPH radical scavenging and reducing power tests were performed to evaluateits antioxidant activities. The cytotoxic activities of the essential oil against a human breast and a uterine cancer cell line were estimated by MTT tests. Additionally, the morphological changes after treatment of the oil fraction were observed under a microscope. The essential oil fraction and its main components, Z-ligustilide and butylidene phthalide, inhibited the growth of three E. coli strains examined, with minimum inhibiting concentrations (MICs) ranging from 1.0 mg/ml to 8.0 mg/ml. Additionally, the essential oil fraction of A. acutiloba exhibited significant DPPH free radical scavenging activity and reducing power. Significant cytotoxic activities of the A. acutiloba essential oil were observed for human uterine (Hela) and breast (MCF-7) cancer cell lines.

• Natural Product Sciences
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• 제19권4호
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• pp.297-302
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• 2013

The current study was conducted to evaluate the antibacterial and antioxidant activities of the essential oil fraction from the roots of Angelica tenuissima Nakai and its main components. We extracted the essential oil fraction from the roots of A. tenuissima using steam distillation and isolated its main components. Their antibacterial activities were determined by broth dilution test against food-borne pathogenic bacteria. Antioxidant activities were evaluated by DPPH-scavenging assay and reducing-power test. Also tested was their ability to inhibit the growth of two gastrointestinal cancer cell lines, Caco-2 and MKN-45. The A. tenuissima oil fraction and its main components, ligustilide and butylidene phthalide exhibited marked inhibitory effects against most of the tested antibiotic-susceptible and antibiotic-resistant bacterial strains with minimum inhibiting concentrations (MICs) from $0.21{\pm}0.08$ to $3.60{\pm}0.89mg/ml$. They also showed growth-inhibiting activity against Caco-2 and MKN-45 cells. The oil fraction showed significant antioxidant activities in DPPH radical scavenging assay and reducing-power test. Taken together, A. tenuissima essential oil could be used as a safe additive for preventing food contamination by pathogenic bacteria. Additionally, its antioxidative activity and the ability to inhibit gastrointestinal carcinoma cell lines could increase its value for functional foods and prevention of cancer.

• Plant Resources
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• 제6권3호
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• pp.183-187
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• 2003

Two kinds of Angelica belong to Umbelliferae collected, the one is Angelica gigas that is inhabitant in Korea and the other is Angelica acutiloba that is indigenous in Japan at the field of Snyder Research and Extension Farm Rutgers University, New Jersey and was analyszed by GC and GC/MS. The composition of the essential oil of the different aerial parts of the Angelica has been studied. The oil yields obtained upon hydrodistillation were 0.18% (v/w) in Korean Angelica and 0.44% (v/w) in Japanese Angelica on dry root weight basis. By the growing stage in the Rutgers greenhouse condition, leaf and root of essential oil content a little decreased on 9 months later than 4 months later except for Angelica gigas leaf. Both of Angelica showed that amounts of essential oil content presented in order of leaf > petiole > root according to different plant part. The analysis of the essential oil from Angelica root led to the identification of 14 constituents totaling 64% in Korean Angelica and 13 constituents totaling 68% in Japanese Angelica. The major constituents of the Angelica root essential oil were ligustilide (47 %) and gamma terpi (14 %) in Korean Angelica, and alpha pinei (32 %) and nonane (25 %) in Japanese Angelica