• Korean Journal of Medicinal Crop Science
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• v.11 no.1
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• pp.71-75
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• 2003

To know the varietal difference and yearly changes of lignan contents in the fruits of collected lines of Schizandra chinensis Baillon, three lignan compounds, schizandrin, gomisin A, and gomisin N, were quantitatively analyzed using High Performance liquid Chromatography. The average contents of schizandrin, gomisin A, and gomisin N, showed 0.78%, 0.25%, and 0.63% in the 6-year-old fruits of 24 lines harvested in 1996, and 0.72%, 0.22%, and 0.63% in the 7-year-old ones of 59 lines harvested in 1997. There were the wide range of coefficient of variation (CV) values, the significant differences by the lines and harvest years, and the significant interaction between line and year for lignan contents. Schizandrin contents during 3 years, $1995{\sim}1997$, showed relatively higher amounts more than 0.9% in Chungju 143, Chungju 453, and Chungju 532. Among 23 lines analyzed from 1995 to 1997, Chungju 542, Chungju 547, and Chungju 580 contained continuously higher amounts more than 0.8% of schizandrin and had lower CV values of lignan contents by the harvest years than the other lines. There were a highly significant and negative correlation between fruit weight and lignan contents, and a positive correlation among contents of schizandrin, gomisin A, gomisin N, and total lignan.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.spc1
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• pp.167-170
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• 2005

To obtain some information on the change of antioxidant components of seeds during grain filling stage as affected by the sowing dates, lignan compounds were investigated according to days of flowering under different sowing dates. Sesamin and sesamolin contents showed significantly different by days of flowering and varieties. Both of sesamin and sesamolin content increased after flowering and reached highest at 40 days of flowering, but they started to decrease thereafter, Sesamin and sesamolin contents of sesame seeds changed with sowing dates. Generally, late sowing date of May 30 showed relatively higher accumulation rate of sesamin and sesamolin contents rather than other sowing dates, but overall patterns were a little different by varieties and lignan compounds. In Yangbaekkae, sesamin and sesamolin contents showed relatively higher at sowing date of May 30, but Yanghuckkae showed higher sesamin and sesamolin contents till 20 days of flowering when sowing date of May 30, but it showed to change that both lignan contents were relatively higher under sowing date of May 10.

• Korean Journal of Organic Agriculture
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• v.24 no.4
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• pp.893-905
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• 2016

Puff processing is one of the preparation and processing methods of herbal medicines. This study analyzed the quality characteristics in schisandra chinensis extracts by puff processing ($1kgf/cm^2$, $3kgf/cm^2$, $5kgf/cm^2$) through changes of the lignan contents and antioxidant activity. The lignan contents and antioxidant activity of puff processing schisandra chinensis extracts (PPSCE) increased higher than compared to a control. Also, as lignan contents and antioxidant activity of PPSCE was increased with the increasing pressure but it was reduced from $5kgf/cm^2$. The results suggest that puff processing was attributed to the quality increase schisandra chinensis.

• Food Science and Biotechnology
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• v.16 no.6
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• pp.981-987
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• 2007

Effects of lignan compounds (sesamol, sesamin, and sesamolin) extracted from roasted sesame oil on the autoxidation at $60^{\circ}C$ for 7 days and thermal oxidation at $180^{\circ}C$ for 10 hr of sunflower oil were studied by determining conjugated dienoic acid (CDA) contents, p-anisidine values (PAV), and fatty acid composition. Contents of lignan compounds during the oxidations were also monitored. ${\alpha}$-Tocopherol was used as a reference antioxidant. Addition of lignan compounds decreased CDA contents and PAY of the oils during oxidation at $60^{\circ}C$ or heating at $180^{\circ}C$, which indicated that sesame oil lignans lowered the autoxidation and thermal oxidation of sunflower oil. Sesamol was the most effective in decreasing CDA formation and hydroperoxide decomposition in the auto- and thermo-oxidation of oil, and its antioxidant activity was significantly higher than that of ${\alpha}$-tocopherol. Sesamol, sesamin, and sesamolin added to sunflower oil were degraded during the oxidations of oils, with the fastest degradation of sesamol. Degradation of sesamin and sesamolin during the oxidations of the oil were lower than that of ${\alpha}$-tocopherol. The results strongly indicate that the oxidative stability of sunflower oil can be improved by the addition of sesamol, sesamin, or sesamolin extracted from roasted sesame oil.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.3
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• pp.203-206
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• 2000

Although lignans of sesame seed, sesamolin and sesamin have been known as possessing an antioxidant activity, it is less known about their contents of the sesame cultivated in Korea. Collections of sesame cultivated in Korea were used for studies on their lignans content of the seed and fatty acids composition of the oil. The sesamin content of sesame seed with white-coat were 370.29 mg/100g seed, while that of sesame seed with black-coat were 246.58mg/100g seed. Also, the sesamolin contents of sesame seed were 202.22 mg/100g seed in white-coat cultivars and 132.68 mg/100g seed in black-coat sesames. Hence, the lignan content of white-coat sesame cultivars was significantly hi임or than that of black-coat ones. Korean sesame cultivars also showed considerably higher sesamin content than sesamolin content in seeds. The correlation between sesamin and sesamolin contents was not recognized in Korean sesame cultivars. The stearic acid of white-coat sesame was significantly higher than that of black-coat one (p＜0.05).

• Korean Journal of Plant Resources
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• v.36 no.4
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• pp.413-434
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• 2023

Sesame (Sesamum indicum L.) is an ancient oilseed crop, which is usually cultivated for its seeds. Sesame breeding aims to achieve high seed yield and quality, along with resistance to biotic or abiotic stresses. It is estimated that sesame is originated from Asia or Africa continent. In this study, we characterized 10 agronomic traits and evaluated lignan contents in 165 sesame germplasm originated from Asia or Africa, to select high-yield or high-lignan content accessions. Sesame germplasm showed diverse phenotypes and highly variable lignan contents (sesamin: 0.5-12.6 mg/g, sesamolin: 0.1-3.5 mg/g, lignan: 1.1-16.1 mg/g). Based on originated continent, there are significant difference in agronomic traits, but no in lignan content. Correlation analysis revealed that yield-related agronomic traits were negatively related with lignan contents. Also, PCA analysis showed that most agronomic traits and lignan contents were principal components explaining diversity of whole sesame germplasm. Sesame germplasm was clustered into three groups based on agronomic traits and lignan contents. Finally, we selected high-yield (IT29416, IT167042, K276848, K276849) and high-lignan candidate accessions (IT169254, IT170031, IT169250, IT154876, IT170034), respectively. These accessions are expected to be valuable resources for breeding of high-yield and high-lignan contents functional cultivars.

• Korean Journal of Medicinal Crop Science
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• v.19 no.6
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• pp.414-420
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• 2011

We investigated antioxidant activity and lignan contents by harvesting times to expand use of Schisandra chinensis (Turcz.) Baillon. Total polyphenol and flavonoid contents of seed was higher than those of flesh but there is not much difference in harvesting times. As $RC_{50}$ value, that was, the concentration of sample required for 50% reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical, was 13.7~24.2 ${\mu}g/m{\ell}$ in seed thus it showed a high antioxidant activity. Among lignan components, schizandrin content was the highest and followed by gomisin N and gomisin A in all of flesh and seed. Also these components in seed were 4~9 times more contained than those of flesh. All of them were decreased by harvesting times in flesh. But the contents of schizandrin and gomisin N were high in August 3rd and September 15th in seed, respectively. As the results, the seed of S. chinensis had high antioxidant activity and lignan contents so it could be potentially developed as a resource.

• Journal of Crop Science and Biotechnology
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• v.10 no.3
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• pp.185-192
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• 2007

Near-infrared spectroscopy(NIRS) was used to develop a rapid and efficient method to determine lignan glucosides in intact seeds of sesame(Sesamum indicum L.) germplasm accessions in Korea. A total of 93 samples(about 2 g of intact seeds) were scanned in the reflectance mode of a scanning monochromator, and the reference values for lignan glucosides contents were measured by high performance liquid chromatography. Calibration equations for sesaminol triglucoside, sesaminol($1{\rightarrow}2$) diglucoside, sesamolinol diglucoside, sesaminol($1{\rightarrow}6$) diglucoside, and total amount of lignan glucosides were developed using modified partial least square regression with internal cross validation(n=63), which exhibited lower SECV(standard errors of cross-validation), higher $R^2$(coefficient of determination in calibration), and higher 1-VR(ratio of unexplained variance divided by variance) values. Prediction of an external validation set(n=30) showed a significant correlation between reference values and NIRS estimated values based on the SEP(standard error of prediction), $r^2$(coefficient of determination in prediction), and the ratio of standard deviation(SD) of reference data to SEP, as factors used to evaluate the accuracy of equations. The models for each glucoside content had relatively higher values of SD/SEP(C) and $r^2$(more than 2.0 and 0.80, respectively), thereby characterizing those equations as having good quantitative information, while those of sesaminol($1{\rightarrow}2$) diglucoside showing a minor quantity had the lowest SD/SEP(C) and $r^2$ values(1.7 and 0.74, respectively), indicating a poor correlation between reference values and NIRS estimated values. The results indicated that NIRS could be used to rapidly determine lignan glucosides content in sesame seeds in the breeding programs for high quality sesame varieties.

• The Korean Journal of Food And Nutrition
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• v.35 no.6
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• pp.543-551
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• 2022

There has been increased interest in lignans due to their potential effect in reducing the risk of developing several diseases. To evaluate lignan contents, sensitive and accurate methods should be developed for their quantification in food. The present study aimed to validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of 5 lignans: lariciresinol (Lar), matairesinol (Mat), pinoresinol (Pin), secoisolariciresinol (Seco), and syringaresinol (Syr). The validation included selectivity, linearity, recovery, accuracy, and precision. The method was proved to be specific, with a linear response (R²≥0.99). The limits of detection were 0.040~0.765 ㎍/100 g and the limits of quantification were 0.114~1.532 ㎍/100 g. Recoveries were 90.588~109.053% for black sesame powder. Relative standard deviations of repeatability and reproducibility were below 5%. Total lignan contents of roasted coffee bean, oat, and blacksoy bean were 105.702 ㎍/100 g, 78.965 ㎍/100 g, and 165.521 ㎍/100 g, respectively. These results showed that LC-MS/MS analysis would be effective in producing acceptable sensitivity, accuracy, and precision in five lignan analyses.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.1
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• pp.95-100
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• 2006

Sesamin and sesamolin, antioxidant lipidsoluble lignan compounds, are abundant in sesame (Sesamum indicum L.) seed oil and provide oxidative stability of oil related to sesame quality. The sesamin and sesamolin contents of 403 sesame land races of Korea were determined by HPLC analysis of methanol extract (HPLC value), and their total lignan content was compared with those by using UV-Vis spectrophotometric analysis (UV method) of methanol (UV-MeOH value) and hexane (UV-Hexane value) extracts. HPLC values of total lignan content were strongly associated with UV-Hexane (r=0.705**) and UV-MeOH (r=0.811**) values. The UV values from both the extracts were 3.8-4.7 times higher than those of HPLC values. Lignan content was overestimated by UV method because total compounds in the mixture solution were quantified by absorbing at the same ultraviolet wavelength as in HPLC method. UV method could more rapidly analyze small amount of sample with higher sensitivity of detection than HPLC method. Average contents of lignans in sesame germplasm evaluated in this study were $2.09{\pm}1.02mg/g$ of sesamin, and $1.65{\pm}0.61mg/g$ of sesamolin, respectively, showing significant variation for lignan components. The results showed that UV method for the determination of sesamin and sesamolin could be practically used as a faster and easier method than HPLC by using the regression equations developed in this study.