• Title/Summary/Keyword: light-chain

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Utilizing Block chain in the Internet of Things for an Effective Security Sharing Scheme

  • Sathish C;Yesubai Rubavathi, C
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.17 no.6
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    • pp.1600-1619
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    • 2023
  • Organizations and other institutions have recently started using cloud service providers to store and share information in light of the Internet of Things (IoT). The major issues with this storage are preventing unauthorized access and data theft from outside parties. The Block chain based Security Sharing scheme with Data Access Control (BSSDAC) was implemented to improve access control and secure data transaction operations. The goal of this research is to strengthen Data Access Control (DAC) and security in IoT applications. To improve the security of personal data, cypher text-Policy Attribute-Based Encryption (CP-ABE) can be developed. The Aquila Optimization Algorithm (AOA) generates keys in the CP-ABE. DAC based on a block chain can be created to maintain the owner's security. The block chain based CP-ABE was developed to maintain secures data storage to sharing. With block chain technology, the data owner is enhancing data security and access management. Finally, a block chain-based solution can be used to secure data and restrict who has access to it. Performance of the suggested method is evaluated after it has been implemented in MATLAB. To compare the proposed method with current practices, Rivest-Shamir-Adleman (RSA) and Elliptic Curve Cryptography (ECC) are both used.

Rab Effector EHBP1L1 Associates with the Tetratricopeptide Repeat Domain of Kinesin Light Chain 1 (Kinesin Light Chain 1 (KLC1)의 Tetratricopeptide Repeat (TPR) 도메인과 Rab effector, EHBP1L1의 결합)

  • Jeong, Young Joo;Park, Sung Woo;Kim, Sang-Jin;Kim, Mooseong;Urm, Sang-Hwa;Lee, Jung Goo;Seog, Dae-Hyun
    • Journal of Life Science
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    • v.30 no.1
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    • pp.10-17
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    • 2020
  • Kinesin-1 is microtubule-dependent plus-end direct molecular motor protein essential for intracellular transport. It is a member of the kinesin superfamily proteins (KIFs) which transport cargo, including organelles, vesicles, neurotransmitter receptors, cell-signaling molecules, and protein complexes through interaction between its light chain subunit and the cargo. Kinesin light chain 1 (KLC1) is a non-motor subunit that associates with the kinesin heavy chain (KHC). Although KLC1 interacts with many different adaptor proteins and scaffolding proteins, its binding proteins have not yet been fully identified. We used the yeast two-hybrid assay to identify proteins that interact with the tetratricopeptide repeat (TPR) domain of KLC1, and found an interaction between KLC1 and EH domain-binding protein 1 like 1 (EHBP1L1). EHBP1L1 bound to the region containing all six TPR repeats of KLC1 and did not interact with KIF5B (a motor protein of kinesin 1) or KIF3A (a motor protein of kinesin 2) in the yeast two-hybrid assay. The carboxyl-terminus of the coiled-coil domain of EHBP1L1 is essential for interaction with KLC1. However, another EHBP1L1 isoform, EHBP1, did not interact with KLC1 in the yeast two-hybrid assay. KLC1 interacted with GST-EHBP1L1 and its coiled-coil domain but not with GST only. When co-expressed in HEK-293T cells, EHBP1L1 co-localized with KLC1 and co-immunoprecipitated with KLC1 and KIF5B but not KIF3A. These results suggest that kinesin 1 motor protein may transport EHBP1L1-associated cargo in cells.

The Role of $Ca^{2+}$/Calmodulin-Dependent Protein Kinase II on the Norepinephrine and GTP-Increased Myosin tight Chain Phosphorylations in Rabbit Mesenteric ${\alpha}-toxin$ Permeabilized Artery (${\alpha}$-독으로 처리한 토끼창간막동맥에서 Norepinephrine과 GTP에 의한 마이오신 인산화의 증가에 대한 $Ca^{2+}$/calmodulin-dependent Protein Kinase II의 역할)

  • Ahn, Hee-Yul;Kim, Hun-Sik;Moreland, Robert S.
    • The Korean Journal of Pharmacology
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    • v.30 no.1
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    • pp.111-116
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    • 1994
  • The role of $Ca^{2+}$/calmodulin-dependent protein kinase II in the increase of myofilament $Ca^{2+}$ sensitivity by agonist and GTP was investigated in rabbit mesenteric ${\alpha}-toxin$ permeabilized artery. $0.3{\mu}M\;Ca^{2+}$ increased myosin light chain phosphorylations monotonically. $10\;{\mu}M$ norepinephrine and $10\;{\mu}M$ GTP potentiated increase of myosin light chain phosphorylations by $0.3{\mu}M\;Ca^{2+}$, which reaches a peak at 5 min and gradually declines to the $Ca^{2+}$ alone level at 20 min. At the early phase (1 min), $10\;{\mu}M$ KN 62, the inhibitor of $Ca^{2+}$/calmodulin-dependent protein kinase II , decreased myosin light chain phosphorylation levels by $10\;{\mu}M$ norepinephrine and $10\;{\mu}M$ GTP in the presence of $0.3{\mu}M\;Ca^{2+}.\;However\;10\;{\mu}M$ KN-62 did not affect the myosin light chain phosphorylations by $10\;{\mu}M$ norepinephrine and $10\;{\mu}M$ GTP in the presence of $0.3{\mu}M\;Ca^{2+}$ at the peak (5 min) and plateau phases (20 min). From these results, the role of $Ca^{2+}$/calmodulin-dependent protein kinase II may be different depending on time, which may play a role in increase of myofilamint $Ca^{2+}$ sensitivity by norepinephrine and GTP resulting from increase of myosin light chain phosphorylations at the early phase. However, at plateau phase, $Ca^{2+}$/calmodulin-dependent protein kinase II may not be involved in the increase of myofilament $Ca^{2+}$ sensitivity by norepinephrine and GTP in rabbit mesenteric ${\alpha}-toxin$ permeabilized artery.

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A Qualitative Study of Food Consumer Behavior Using the Means-end Chain Method - Illustrating Research into Swede's Perception on Asian Food - (연상네트워크기법(Means-end Chain method)을 이용한 식품소비자행동 질적 연구 - 스웨덴인의 아시아 음식 인식을 중심으로 -)

  • Lee, Kyung-Ran;Mengoni, Mariangela;Mayazi, Adja;Pehrsson, Anders
    • Journal of the Korean Society of Food Culture
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    • v.24 no.6
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    • pp.641-648
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    • 2009
  • The principal objective of this study was to analyze Swede's perceptions of Asian food using a means-end chain method, which may contribute to our understanding of new markets for a food industry interested in globalizing Korean food. With the Means-end chain method, one can determine the cognitive structures built in consumer's minds, which are developed by connecting attributes of product, consequences, and values. The attributes of Asian food that are most positively perceived by Swedes are 'fresh vegetables', 'low-fat', 'light meat and seafood', 'exotic ingredients', 'not expensive' and 'unknown food'. The consequences of eating Asian food connected to these attributes are 'healthy', 'tasty', 'good way to save money' and 'curious'. Finally, Swedes expect to enjoy a value of 'achievement' at the end. Based on the result that 'unknown food' attribute is connected to 'achievement' value, Swedes are assumed to be attracted by the fact that Korean food is 'unknown food'. However, the effect of the 'unknown food' attribute will fade away with time; therefore, stressing Korean food's status as a 'healthy' food, which can be attributed to its use of 'fresh vegetable' and 'light meat and seafood' ingredients and 'low fat' cooking method may be effective as a long-term strategy for making Korean food attractive. The 'healthy' consequence is connected to Swedes' perception of the value 'achievement' and simultaneously to their perception of the value 'belonging and love/sense of belonging'.

Genomic Organization, Intronic Duplications, and Promoter Characteristics of the Fast Skeletal Myosin Light Chain-2 Gene (mlc2f) from Javanese Ricefish Oryzias javanicus

  • Lee, Sang Yoon;Kim, Dong Soo;Nam, Yoon Kwon
    • Fisheries and Aquatic Sciences
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    • v.15 no.4
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    • pp.325-335
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    • 2012
  • The present study characterized the fast skeletal myosin light chain-2 gene (mlc2f) in the euryhaline Javanese ricefish (Oryzias javanicus: Beloniformes). Coding nucleotide and deduced amino acid sequences of Javanese ricefish mlc2f were well conserved in the vertebrate lineage. Javanese ricefish mlc2f showed a typical seven-exon structure, and its promoter exhibited transcription factor binding motifs common to most muscle-specific genes. However, Javanese ricefish mlc2f also displayed tandem duplications of intronic sequences in both intron 1 and intron 3. Based on quantitative reverse transcription-polymerase chain reaction, the mlc2f transcripts were highly predominant in skeletal muscles of adults and were differentially modulated during embryonic development. Microinjection of the mlc2f promoter-driven red fluorescent protein (RFP) reporter construct successfully exhibited heterologous expression of the fluorescent reporter, primarily in muscular areas of hatchlings, although the distribution pattern of RFP signals was not uniform due to the mosaic nature of the introduced transgene. Data from this study indicate that the Javanese ricefish mlc2f gene has undergone "intra-intronic" duplication events in a species-specific manner and that the mlc2f regulator may also be useful in heterologous expression assays of the skeletal muscles of this species.

IMAGE DATA CHAIN ANALYSIS FOR SATELLITE CAMERA ELECTRONIC SYSTEM

  • Park, Jong-Euk;Kong, Jong-Pil;Heo, Haeng-Pal;Kim, Young-Sun;Chang, Young-Jun
    • Proceedings of the KSRS Conference
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    • v.2
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    • pp.791-793
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    • 2006
  • In the satellite camera, the incoming light source is converted to electronic analog signals by the electronic component for example CCD (Charge Coupled Device) detectors. The analog signals are amplified, biased and converted into digital signals (pixel data stream) in the video processor (A/Ds). The outputs of the A/Ds are digitally multiplexed and driven out using differential line drivers (two pairs of wires) for cross strap requirement. The MSC (Multi-Spectral Camera) in the KOMPSAT-2 which is a LEO spacecraft will be used to generate observation imagery data in two main channels. The MSC is to obtain data for high-resolution images by converting incoming light from the earth into digital stream of pixel data. The video data outputs are then MUXd, converted to 8 bit bytes, serialized and transmitted to the NUC (Non-Uniformity Correction) module by the Hotlink data transmitter. In this paper, the video data streams, the video data format, and the image data processing routine for satellite camera are described in terms of satellite camera control hardware. The advanced satellite with very high resolution requires faster and more complex image data chain than this algorithm. So, the effective change of the used image data chain and the fast video data transmission method are discussed in this paper

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Studies on the Isolation and Immunochemical Properties of SIgA from Human and Bovine Milk (인유(人乳) 및 우유(牛乳)로부터 Secretory Immunoglobulin A의 분리(分離) 및 면역화학적(免疫化學的) 특성(特性)에 관(關)한 연구(硏究))

  • Lee, Jo Yoon;Kim, Jong Woo
    • Korean Journal of Agricultural Science
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    • v.22 no.1
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    • pp.82-95
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    • 1995
  • These experiments were carried out to isolate SIgA from human and bovine milk. The immunochemical properties of SIgA from human and bovine milk were examined by Gel filtration, DEAE and SDS-PAGE. Double Immunodiffusion, and Immunoelectrophoresis. The results obtained are as follows: 1. Human SIgA was purified from colostrum of Korean women by repeated gel filtration on Sephadex G-200 and Sepharose 6B, but bovine SigA was not cleary purified from bovine colostrum of Holstein cows by anion exchange chromatography using DEAE-Sephadex A-50 and gel filtration on Sephadex G-200 and Sepharose 6B. 2. The immunochemical properties of fractions from gel filtration on the Sephadex G-200 and Sepharose 6B column as assessed by Immunoelectrophoresis and double Immunodiffusion to identify the presence of IgM in first peak fraction, and the presence of pure SIgA in second peak fraction. However, Bovine SigA rich fraction from bovine colostrum of Holstein cows contained a large amount of $IgG_1$-dimer in addition to SIgA. 3. The fragments of reduced bovine colostrum SIgA rich fraction were estimated to have molecular weights of secretory component, heavy chain and light chain (75,000-80,000, 50,000-60,000, 25,000-27,000 daltons) by SDS-PAGE, respectively. Those were similar to the molecular weight of reduced SIgA from human milk.

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NMR Characterization of Oxidized Form of Human 8-kDa Dynein Light Chain

  • Shin, Jae-Sun;Jeong, Woo-Jin;Chi, Seung-Wook
    • Journal of the Korean Magnetic Resonance Society
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    • v.14 no.2
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    • pp.127-133
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    • 2010
  • Redox-dependent conformational change of human 8-kDa Dynein light chain (LC8) plays important role in regulating NF-${\kappa}B$ signaling pathway. In this study we characterized the structural states of the oxidized and reduced forms of LC8 by using NMR spectroscopy. The $^1H-^{15}N$ 2D HSQC spectra of oxidized LC8 indicated that no significant change in tertiary structure of LC8 occurred upon oxidation. The chemical shift perturbations of LC8 upon oxidation suggest a redox-dependent quaternary structural change.

Liquid Crystal Alignment and Generation of Pretilt Angle by Using Photo-alignment Techniques on Different Polymer Molecules (광배향기술을 이용한 액정배향의 기구 및 폴리이미드의 분자구조가 프리틸트각에 미치는 영향)

  • 서대식;황율연;이창훈
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.11 no.6
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    • pp.477-480
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    • 1998
  • In this paper, we investigated the liquid crystal(LC) alignment and generation of pretilt angle by using photo-alignment techniques on two kinds of polyimide(PI) surface, It was found that the uniform alignment for nematic(N) LC is obtained in a cell with slanted UV light irradiation on PI surface without side chain. We successfully observed that the pretilt angle of NLC is generated about $3^{\circ}$ with an incident angle of 70 degree on the PI surface without side chain. It is considered that the pretilt angle generation in NLC is attributed to interaction between the LC molecules and the polymer surfaces.

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Electrical Conductivity and Optical Properties of Elongated Conducting Polymer (연신도전성고분자의 전기전도와 광학적 특성)

  • 박대희;강성화;임기조
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 1994.05a
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    • pp.59-62
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    • 1994
  • Tact Electrical conductivity. optical absorption spectra of poly(p-phenylene vinylene) and their dependence on stretching are discussed in detail. The conductivity in the parallel direction to the stretching is higher over one order in magnitude than that in the perpendicular direction to the stretching is higher over one order in magnitude than that in the perpendicular direction to the stretching. The photocurrent spectrum for the ligh polarized parallel to the chain direction is much enhanced in lower photon energy compared with that for the light polarized perpendicular to that direction. The result may be originated in the difference of energies which is needed to photogenerate carriers wi th the light polarized parallel and perpendicular to the chain stretched direction.

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