• Title/Summary/Keyword: light utilization

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Enhanced pectinase and β-glucosidase enzyme production by a Bacillus subtilis strain under blue light-emitting diodes

  • Elumalai, Punniyakotti;Lim, Jeong-Muk;Oh, Byung-Teak
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.10a
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    • pp.109-109
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    • 2018
  • Bacillus subtilis B22, a chemotrophic and aerobic bacterial strain was isolated from homemade kimchi, identified by 16S rRNA gene sequencing. B22 was primarily screened by biochemical, carbon source utilization tests. B22 was used to produce pectinase and ${\beta}$-glucosidase by submerged fermentation under different light sources. B22 was incubated in pectin media and basal media (pH 7.0) under blue, green, red and white light-emitting diodes (LEDs), fluorescent white light, and in darkness at $37^{\circ}C$, orbital shaker 150 rpm for 24 hours. Fermentation under blue LEDs maximized pectinase production ($71.59{\pm}1.6U/mL$ at 24 h) and ${\beta}$-glucosidase production ($56.31{\pm}1.6U/mL$ at 24 h). Further, the production of enzyme increased to pectinase ($156{\pm}1.28U/mL$) and ${\beta}$-glucosidase ($172{\pm}1.28U/mL$) with 3% glucose as a carbon source. Activity and stability of the partially purified enzymes were higher at pH 6.0 to 8.0 and $25-55^{\circ}C$. The effect on the metal ions $Na^+$ and $K^+$ and (moderateactivity) $Mn^{2+}$ and $Ni^{2+}$ increased activity, while $Hg^{2+}$, $Cu^{2+}$, $Fe^{2+}$, and $Fe^{2+}$ inhibited activity. EDTA, phenylmethylsulfonyl fluoride and 5,5-dithiobis (2-nitrobenzoicacid) reduced activity, while tetrafluoroethylene and 1,10-phenanthroline inhibited activity. The amylase was highly tolerant of the surfactants TritonX-100, Tween-20, Tween-80 and compatible with organic solvents methanol, ethanol, isoamylalcohol, isopropanol, t-butylalcohol and the oxidizing agents hydrogen peroxide, sodium perborate and sodium hypochlorite, although potassium iodide and ammonium persulfate reduced activity. These properties suggest utility of pectinase and ${\beta}$-glucosidase produced by B. subtilis B22 under blue LED-mediated fermentation for industrial applications.

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A Study on Characterization of Expansion Agent in Mortar with Light Burned Dolomite By-Product (경소백운석(輕燒白雲石) 부산물(副産物)을 활용(活用)한 바닥 몰탈용 팽창재(膨脹材)의 특성검토(特性檢討))

  • Lee, Keon-Ho;Min, Sung-Eoi;Lee, Hyoung-Woo;Cho, Jin-Sang;Cho, Kye-Hong;Han, Choon
    • Resources Recycling
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    • v.21 no.6
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    • pp.12-22
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    • 2012
  • This study investigated a utilization technology of light burned dolomite. light burned dolomite ($CaMg(CO_3)_2$) generated in furnace (steel manufacturer) is an alternative to quick lime. Using light burned dolomite has an effect on reducing the consumption of fluorite slag MgO concentration in supersaturated solution by prolonging the life of softening effect. Armophous MgO, not containing periclase is formed by firing dolomite under $800^{\circ}C$. It has larger surface area and higher reactivity than periclase, and also shows better expansion effect than quicklime. Due to those effects, therefore, armophous MgO produced from light burned dolomite is used as an alternative expansion agent in mortar. In the experiment, characteristics of light burned dolomite were compared to those of existing expansion agents such as anhydrite and quicklime. Then, each expansion and shrinkage rates were measured over a period of about 3 months in both of 1m Jis mold at labscale and apartment mortar flooring at field scale. In the result, it was observed that light burned dolomite in mortar flooring more compensates for the expansion and shrinkage rates than the existing expansion agents, showing low expansion rate of below 0.05% and also decreases the crack.

A Case Study of Space Utilization on Compact Houses in Japan to build Compact-but-effective Houses in Korea (강소주택 실효성 논의를 위한 일본 소형주택 공간 활용 사례 분석)

  • Seo, Min-Woo;Han, Young-Ho
    • Korean Institute of Interior Design Journal
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    • v.21 no.1
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    • pp.148-158
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    • 2012
  • The objective of this study is to find a possible application of small space utilization of GangSo Housing, so called compact-but-effective housing, through analyzing that of Japanese small housing. We analyze openness of view and flexibility of spaces divided by the physical and architectural aspects as first component and the psychological and interior space aspects as second component. The results showed that Japanese small houses have various unit plan compared to uniformity of Korean houses. Openness of view in Japanese small housing is accomplished by letting in light from the outside using position and shape of the window, looking more spacious using courtyard, void spaces, or sliding door hanging from the ceiling, and creating deception of view using floor-wall plan and appropriate materials. Flexibility of spaces is achieved by combination of first and second components, multipurpose of space and furniture, and variety of storage methods. It is necessary to improve spatial efficiency with consideration of volume-metric planing rather than flat-plane and develop various unit plans to meet residents' needs and demands on compact-but-effective houses.

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An Observational Study on the Behaviors of 4-to 5-Year-Olds in the Cooperative/Competitive Situation (경쟁/협동상황에서의 4-5세 아동의 상호작용 관찰 연구)

  • Kim, Pan Hee
    • Korean Journal of Child Studies
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    • v.17 no.1
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    • pp.5-21
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    • 1996
  • The purpose of the present study was to investigate how 4-to 5-year-olds approach a competitive/cooperative task to obtain a resource. Sex and age differences were examined for the behavior and resource utilization of this task. The subjects were 74 same-age, mixed sex groups of 4 children each in three kindergartens in a large city. Each group of 4 children were classmates. The children were videotaped in a play situation where one child could view a cartoon movie (i. e. acquiring a resource) with the assistance of two children (one to turn the film tape and one to push the light-button), and the fourth child was a bystander. Time at each position and physical and verbal behavior constitute the data which were analyzed by ANOVA and ${\chi}^2$. The results of the time analysis showed that children cooperated quickly and were able to view the cartoon(resource utilization) for a large part of the session. High resource utilizers compared with low resource utilizers, exhibited more self-assertive and helping behaviors. Boys achieved more viewing time while girls were bystanders for a greater amount of time. Boys showed more physical and verbal behaviors during the task. Four-year-olds achieved less viewing time than 5-year-olds, indicating 4-year-olds were less effective in eliciting the help of their peers.

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A Study on the Development of PV Application for Apartment Buildings (공동주택을 위한 PV 시스템 적용기법 개발 연구)

  • Noh, Ji-Hee;Yoon, Chul;Yi, So-Mi;Joo, Man-Sik
    • 한국태양에너지학회:학술대회논문집
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    • 2008.04a
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    • pp.269-274
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    • 2008
  • Nowaday, The Sustainable Development about global environment is the most important subject. In urban environment, a variety of the nature energy utilization such as the solar energy are the most efficient solution to solve this issue. One of these efficient, solutions, a photovoltaic system using sunlight has been introduced to the building with an advantage such as cost-effective, safe for using and good for environment friendly in light with energy utilization. Recently, many of the apartment housings are built in domestic country. The apartment buildings have been constructed since early of 1970's. now apartment is taking over 50% out of entire housing in korea. The apartment housing applying to a photovoltaic system has been extensively studied in the foreign country but our county runs short. So, It was necessary to technical development of PV application which is suitable in Korean house culture. The objective of this study is to develop the building integrated PV application method for apartment building.

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Effect of Synthesis Method and Melamine Content of Melamine-Urea-Formaldehyde Resins on Bond-Line Features in Plywood

  • LUBIS, Muhammad Adly Rahandi;JEONG, Bora;PARK, Byung-Dae;LEE, Sang-Min;KANG, Eun-Chang
    • Journal of the Korean Wood Science and Technology
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    • v.47 no.5
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    • pp.579-586
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    • 2019
  • This work examined effects of the synthesis method and melamine content of melamine-urea-formaldehyde (MUF) resins on the bond-line features (i.e. resin penetration and bond-line thickness) in plywood. Two synthesis methods (MUF-A and MUF-B) and three melamine contents (5, 10, and 20%) were employed to prepare MUF resins. The MUF-A resins at three melamine contents were prepared by a simultaneous reaction of melamine, urea, and formaldehyde, while the MFU-B resins were prepared by reacting melamine at the same levels with formaldehyde followed by urea. The results showed that higher melamine content increased the viscosity of MUF-A and MUF-B resins. The resin penetration of MUF-A resins decreased by 48% while those of MUF-B resins increased by 16% at 20% melamine content. As a result, the MUF-A resins had greater bond-line thickness than those of MUF-B resins as the melamine content increased. The MUF-B resins resulted in thinner bond-line and greater resin penetration compared to those of MUF-A resins. The results suggested that MUF-B resins prepared with 20% melamine content had a proper combination of resin penetration and bond-line thickness that could produce plywood panel with a better adhesion performance.

Primary production by phytoplankton in the territorial seas of the Republic of Korea

  • An Suk, Lim;Hae Jin, Jeong
    • ALGAE
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    • v.37 no.4
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    • pp.265-279
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    • 2022
  • The primary production (PP) by phytoplankton in marine ecosystems is essential for carbon cycling and fueling food webs. Hence, estimating the PP in the territorial sea of each country is a necessary step to achieving carbon neutrality. To estimate the PP in the territorial sea of the Republic of Korea from 2005 to 2021, we analyzed various physiochemical parameters, such as sea surface temperature (SST), Secchi depth, and concentrations of chlorophyll-a and nutrients in the seas of five regions, including the East Sea, West Sea, western South Sea, eastern South Sea, and the waters off Jeju Island. During the 17-year study period, the SST tended to increase, while the nutrient concentrations declined, except in the Jeju area. Overall, the PP did not show a specific temporal trend, but daily PP in the western South Sea was the highest among the five regions. Moreover, the maximum PP in the Korean territorial waters (76,450 km2) was estimated at 11,227 Gg C y-1, which accounts for 0.03% of the global PP. The results may give insights into a better understanding of the PP, further resource utilization, and environmental sustainability in the studied region.

Algae Culture Characteristics Viewed with Continuous and Cyclic Irradiation in High Rate Algae Biomass Culture Pond (고율 조류 생세포체 배양지에서 조사 조건으로 본 조류 배양 특성)

  • 공석기
    • Journal of environmental and Sanitary engineering
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    • v.14 no.3
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    • pp.123-129
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    • 1999
  • The utilization methods of algae biomass have been studied constantly in whole world. These are $\circled1$the wastewater treatment if waste stabilization pond and oxidation ditch etc. and $\circled2$the biosorption of heavy metals and recovery of strategic' precious metals and $\circled3$the single-celled protein production and the production of chemicals like coloring agent and $\circled4$the production of electric energy through methane gasification. The culture system also has been developed constantly in relation with such utilization method developments. In the result of experimental operation under continuous and cyclic irradiation of light, using high rate algae biomass culture pond(HRABCP), which had been made so as to be an association system with the various items which had been managed to have high efficiency for algae culture, the algae production of the 12 hours-irradiance pond was 41.48 Chlorophyll-a ${\mu}g/L$ only in spite of having the more chance of $CO_2$ synthesis to algae cell than the 24 hours-irradiance pond. This means that the energy supply required for dark-reaction of photosynthesis is very important like this. The difference of algae production between continuous and cyclc irradiation explains that the dark-reaction of photosynthesis acts on algae production as the biggest primary factor. The continuous irradiance on HRABCP made the good algae-production($1403.97{\;}{\mu}g$ Chlorophyll-a/mg) and the good oxygen-production(5.8 mg $O_2/L$) and the good solid-liquid seperation. especially, DO concentration through the oxygen-production was enough to fishes' survival.

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Review on Rice Flour Manufacturing and Utilization

  • Kim, Myoung Ho
    • Journal of Biosystems Engineering
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    • v.38 no.2
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    • pp.103-112
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    • 2013
  • Background: The Korean government launched a project in 2008, where the amount of rice used as raw ingredient in rice-based foods in 2012 was planned to increase up to 10% (470,000 ton) of the total rice production through developing various new rice-based processed foods and their commercial manufacturing technology. Among the four major rice-based processed foods, rice cakes and noodles need rice flour as their main raw ingredient. Technology in rice flour utilization and manufacturing is far behind than the technology pertinent to wheat flour in many subject areas. Purpose: This review aims to provide information on rice flour utilization and manufacturing with some fundamental subjects in the area of size reduction. Results: A variety of food items including bread, noodle, cake, cookie, muffin, pre-mix, beverage, vinegar, surimi, and artificial meat have found rice flour as their raw ingredient. Rice bread made out of 100% rice flour has been developed and is now sold in retail stores. Various noodle products made from rice flour are also on the market. Issues on product definition and labeling regulation about rice flour content of the products were explored. Generalized grinding equations available in the literature were seldom used in practice; instead, it has been a general practice to develop empirical equations from test milling data. Introductory remarks on three popular particle size measurement methods (sieving, Coulter counter, light diffraction) were explained. Mathematical expressions frequently used to describe particle size distribution and to correlate cumulative quantity of particles with particle size were represented. Milling methods used in producing rice flour were described along with their advantages and disadvantages. Because of their profound effect on functional properties of the rice flour, four rice flour milling equipments used at both laboratory experiments and commercial manufacturing plants were discussed.

The Impacts of Private Health Insurance on Medical Institution Selection: Evidence from Outpatient Service Utilization among Arthritis Patients (민간의료보험이 의료기관 종별 선택에 미치는 영향: 관절염 환자의 외래 이용을 중심으로)

  • You, Chang Hoon;Kang, Sungwook;Choi, Ji Heon;Kwon, Young Dae
    • Korea Journal of Hospital Management
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    • v.22 no.2
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    • pp.58-69
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    • 2017
  • Recently, with the increase in the number of private health insurance subscribers, interest in overuse of the medical service is increasing. This study analyzed the impacts of private health insurance (PHI) on medical institution selection in outpatient service utilization among persons with arthritis. In order to control patients' health status, we extracted outpatient episodes with the same disease (KCD6, M13) from Korea Health Panel. The unit of analysis was an outpatient visit with arthritis in 2014 (n=23,363). In the light of insurance coverage, we redefined three type of private health insurance (ex, indemnity, fixed benefit, and non-insured) as a test variable and two type of medical institution (ex, hospital and physician visit) as a dependent variable. We conducted a probit regression analysis to identify the impacts of PHI on medical institution selection controlling for heteroscedasticity. The results of this study showed that the insured with indemnity were more likely to choose hospital departments than clinics (marginal effect=0.0475, p=0.000). The impact of participation of fixed benefit PHI was not as clear as that of indemnity type (marginal effect=0.0162, p=0.047). In conclusion, this study confirmed that PHI, particularly indemnity type has a significant impact on the selection of medical institutions. Healthcare policy makers should consider that PHI not only affects the overall quantitative increase in healthcare utilization, but also influences the selection of medical institutions.