• Plant Resources
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• v.8 no.3
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• pp.181-187
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• 2005

A cinnamoyl CoA reductase (CCR) cDNA (ClCCR) was isolated from tabroot mRNAs of Codonopsis lanceolata by cDNA library construction, and its expression was investigated in relation to abiotic stresses. The ClCCR is 1008 bp in length with an open reading frame (ORF) of 336 amino acids. The deduced amino acid sequence was showed high similarity with cinnamoyl-CoA reductases of P. tremuloides (AAF43141) 87%, F.${\times}$aranassa (AAP46143) 83%, L. album (CAD29427) 80%, E. gunnii (CAA66063) 72%, S. tuberosum (AAN71761) 83%. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis was revealed that the ClCCR expression was regulated by abiotic stresses.

• Proceedings of the KSR Conference
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• 2002.10a
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• pp.733-738
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• 2002

In this study, contact fatigue in wheel-rail contact is simulated. It is necessary to calculate contact stress and subsurface stresses accurately to predict fatigue behavior. Contact stresses are obtained by contact analysis of semi-infinite solid based on influence function and subsurface stress field obtained by using rectangular patch solutions. Based on these stress values, several multiaxial high-cycle fatigue criteria are used and the critical loads corresponding to fatigue limits are calculated. The simulation results show that the critical load is decreasing rapidly and the site of crack initiation also moves rapidly to the surface from the subsurface when friction coefficient exceeds a specific value for all of three fatigue criteria.

• Structural Engineering and Mechanics
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• v.27 no.1
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• pp.33-48
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• 2007

An Articulated tower is one of the compliant offshore structures connected to the sea-bed through a universal joint which is the most vulnerable location of the tower that sustains the randomly fluctuating shear stresses. The time history response of the bottom hinge shear is obtained and presented in the spectral form. The fatigue and fracture reliability assessment of the tower joint against randomly varying shear stresses have been carried out. Non-linear limit state functions are derived in terms of important random variables using S-N curve and fracture mechanics approaches. Advanced First Order Reliability Method is used for reliability assessment. Sensitivity analysis shows the influence of various variables on the hinge safety. Fatigue life estimation has been made using probabilistic approach.

• Proceedings of the Korean Reliability Society Conference
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• 2006.05a
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• pp.48-54
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• 2006

In this thesis, accelerated life test (ALT) method and procedure for rubber O-ring are applied to assure specified reliability of the products at guaranteeing the life of the products. Rubber O-ring is parts that keep intensity or make machine operation smoothly on attrition portion of machine and is used to prevent that oil is leaked. Usually. Rubber O-ring used NBR that is copolymer of acrylonitrile and butadiene. this are superior oil resistance, heat resistance, durability of abrasion, cold resistance, chemical resistance etc. The accelerated life test model for rubber O-ring are developed using the relationship between stresses and life characteristics of products. Using the accelerated life test method and the acceleration life test equipment which is developed, we performed life test, collected life data and analyzed the results of tests. The proposed accelerated life test method and procedure may be extended and applied to testing similar kinds of products to reduce test times and costs of the tests remarkably.

• Journal of Plant Biotechnology
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• v.38 no.2
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• pp.162-168
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• 2011

High salinity is a common stress condition that adversely affects plant growth and crop production. In response to various environmental stresses, plants activate a number of defense genes that function to increase the tolerance. To isolate Arabidopsis genes that are involved in abiotic stress responses, we carried out genetic screening using various mutant lines. Among them, the blh8 ($\b{B}$EL1-$\b{L}$ike $\b{H}$omeodomain $\underline{8}$) mutant specifically shows chlorotic phenotypes to ionic (specifically, $Na^+$ and $K^+$) stresses, but no differences in root growth. In addition, BLH8 is related to plant development and abiotic stress as predicted by a Graphical Gaussian Model (GGM) network program. It implies that BLH8 functions as a putative transcription factor related to abiotic stress responses. Collectively, our results show that gene network analysis is a useful tool for isolating genes involved in stress adaptation in plants.

• Structural Engineering and Mechanics
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• v.49 no.2
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• pp.225-235
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• 2014

Cold expansion is an efficient way to improve the fatigue life of an open hole. In this paper, three finite element models have been established to bind the crack growth from an expanded hole and simulated. Expansion and its degree influence are studied using a numerical analysis. Stress intensity factors are determined and used to evaluate the fatigue life. Residual stress field is evaluated using a nonlinear analysis and superposed with the applied stresses field in order to estimate fatigue crack growth. Experimental tests are conducted under constant loading. Results of this investigation indicate expansion and its degree are beneficial to fatigue life and a good agreement was observed between FEM simulations and experimental results.

• Proceedings of the KSME Conference
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• 2004.11a
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• pp.7-12
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• 2004

A lifetime prediction of holddown spring screw in nuclear fuel assembly was performed using fracture mechanics approach. The spring screw was designed such that it was capable of sustaining the loads imposed by the initial tensile preload and operational loads. In order to investigate the cause of failure and to predict the stress corrosion cracking life of the screw, a stress analysis of the top nozzle spring assembly was done using finite element analysis. The elastic-plastic finite element analysis showed that the local stresses at the critical regions of head-shank fillet and thread root significantly exceeded than the yield strength of the screw material, resulting in local plastic deformation. Normalized stress intensity factors for PWSCC life prediction was proposed. Primary water stress corrosion cracking life of the Inconel 600 screw was predicted by using integration of the Scott model and resulted in 1.78 years, which was fairly close to the actual service life of the holddown spring screw.

• Proceedings of the KSME Conference
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• 2004.11a
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• pp.148-153
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• 2004

This paper represents that parts of a large generator operating in 1000 MW are affected by centrifugal forces due to high-speed rotation in 3600 rpm and 3D FEM Analyses are required to obtained the structural reliability of the generator. From these results, one would know the weakest locations and the stress distributions. The fatigue life is calculated in order to grasp the remaining life of generator. 2D and 3D analyses are performed to calculate stresses of the generator rotor and the retaining ring. From 2D results, we find the SCF at the slot and sub-slot of the rotor. 3D analysis is applied at the end part of generator rotor, which represents complex geometry, and rotor and retaining ring shrink thermally. With these results, designers of rotor and retaining ring can compare with the results of design code and verify the stress distributions of generator rotor and retaining ring, and then calculate the remaining life from the low-cycle fatigue data.

• Proceedings of the KSME Conference
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• 2007.05a
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• pp.768-773
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• 2007

This paper presents the numerical estimation of the fatigue life for the welded parts of the engine frame box of the S60MC-C vessel engine. The time-variations of the effective stresses at the critical points during a piston cycle are computed through the finite element analysis, by applying the dynamic loadings that were analytically derived by the kinematic analysis. The fatigue life of the welded parts is estimated by making use of the hot-spot stress extrapolation and the Palmgrem-Minor cumulative damage rule.

• Journal of the Korean Society for Railway
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• v.4 no.4
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• pp.138-146
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• 2001

For the purpose of the effective maintenance of surface irregularities of rail, this paper investigates the rail service life to a bending fatigue failure of welding parts of rail on the slab track by the structures laboratory testing to derive S-N curves, a GTDAP(General Track Dynamic Analysis Program) to calculate the stresses and a modified Miner's rule to predict a fatigue life. The useful guides for maintenance of rail welded parts on the slab track are proposed in terms of a grinding period and the depth of rail surface irregularities. This study also proposed the guides for maintenance of rail welded part on the ballast track. The effects of fatigue service lives are compared one another.