• 제목/요약/키워드: life stresses

검색결과 767건 처리시간 0.03초

자동차용 Leaf 스프링 재질의 마찰 및 마멸 특성 (The Characteristics of Friction and Wear for Automative Leaf Spring Materials)

  • 오세두;안종찬;박순철;정원욱;배동호;이영제
    • 한국윤활학회:학술대회논문집
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    • 한국윤활학회 2003년도 학술대회지
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    • pp.118-126
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    • 2003
  • In the present study, the residual stresses can have a significant on the life of structural engineering components. Residual stresses are created by the surface treatment such as shot peening or deep rolling. The objective of this experimental investigation is to study the influence of friction and wear characteristics due to residual stress under dry sliding condition. Friction and wear data were obtained with a specially designed tribometer. Test specimens were made of SUP9(leaf spring material) after they were created residual stress by shot peening treatment. Residual stress profiles were measured at surface by means of the X-ray diffraction. Sliding tests were carried out different contact pressure and same sliding velocity 0.035m/s(50rpm). Leaf spring assembly test used to strain gauge sticked on leaf spring specimen in order to measure interleaf friction of leaf spring. Therefore, we were obtained hysteresis curve. As the residual stresses of surfaces increased, coefficient of friction and wear volume are decreased, but the residual stresses of surfaces are high, and consequently wear volume do not decreased. Coefficient of friction obtained from leaf spring assembly test is lower than that obtained from sliding test. From the results, structural engineering components reduce coefficient of friction and resistant wear in order to have residual stresses themselves.

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A pathogen-induced osmotin-like protein gene, CAOSMl, from pepper: Differential expression and in situ localization in pepper tissues during pathogen infection and abiotic stresses

  • Hong, J.K.;Jung, H.W.;Lee, B.K.;Lee, S.C.;Hwang, B.K.
    • 한국식물병리학회:학술대회논문집
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    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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    • pp.78.1-78
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    • 2003
  • An osmotin-like protein (CAOSMl) gene was isolated from pepper leaves infected with the avirulent strain Bv5-4a of Xmthomonas campestris pv. vesicatoria. The cDNA encodes a polypeptide of 250 amino acids with a molecular mass of 27, 361 Da. Its amino acid sequence is highly homologous to various osmotin-like proteins from other plant species. The CAOSMl gene expression was organ- and tissue-specifically regulated In pepper plants. The CAOSMl mRNA was intensely localized in the endodermis area of root tissue and in the phloem cells of vascular bundles of red fruit tissue, but not in leaf, stem, and green fruit tissues of healthy pepper plants. Infection by X. c. pv vesintoria, Colletotrichum coccodes, or Phytopkhora capsici iinduced CAOSMl transcription in the leaf or stem tissues. Expression of the CAOSMl gene was somewhat higher in the incompatible than the compatible interactions of pathogens with pepper. The CAOSMl mRNA was prevalently localized in the phloem cells of the vascular bundle of leaf tissues infected by C. coccodes. The CAOSMl gene was activated in leaf tissues by treatment with ethylene, methyl jasmonate, high salinity, cold acclimation and mechanical wounding, but not by abscisic acid (ABA) and drought. These results indicate that the pepper CAOSMl protein functions in response to Pathogens and some abiotic stresses in pepper plants

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Overexpression of a Pathogenesis-Related Protein 10 Enhances Biotic and Abiotic Stress Tolerance in Rice

  • Wu, Jingni;Kim, Sang Gon;Kang, Kyu Young;Kim, Ju-Gon;Park, Sang-Ryeol;Gupta, Ravi;Kim, Yong Hwan;Wang, Yiming;Kim, Sun Tae
    • The Plant Pathology Journal
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    • 제32권6호
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    • pp.552-562
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    • 2016
  • Pathogenesis-related proteins play multiple roles in plant development and biotic and abiotic stress tolerance. Here, we characterize a rice defense related gene named "jasmonic acid inducible pathogenesis-related class 10" (JIOsPR10) to gain an insight into its functional properties. Semi-quantitative RT-PCR analysis showed up-regulation of JIOsPR10 under salt and drought stress conditions. Constitutive over-expression JIOsPR10 in rice promoted shoot and root development in transgenic plants, however, their productivity was unaltered. Further experiments exhibited that the transgenic plants showed reduced susceptibility to rice blast fungus, and enhanced salt and drought stress tolerance as compared to the wild type. A comparative proteomic profiling of wild type and transgenic plants showed that overexpression of JIOsPR10 led to the differential modulation of several proteins mainly related with oxidative stresses, carbohydrate metabolism, and plant defense. Taken together, our findings suggest that JIOsPR10 plays important roles in biotic and abiotic stresses tolerance probably by activation of stress related proteins.

Advances in the molecular breeding of forage crops for abiotic stress tolerance

  • Alam, Iftekhar;Kim, Kyung-Hee;Sharmin, Shamima Akhtar;Kim, Yong-Goo;Lee, Byung-Hyun
    • Journal of Plant Biotechnology
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    • 제37권4호
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    • pp.425-441
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    • 2010
  • Forages are the backbone of sustainable agriculture. They includes a wide variety of plant species ranging from grasses, such as tall fescue and bermudagrass, to herbaceous legumes, such as alfalfa and white clover. Abiotic stresses, especially salinity, drought, temperature extremes, high photon irradiance, and levels of inorganic solutes, are the limiting factors in the growth and productivity of major cultivated forage crops. Given the great complexity of forage species and the associated difficulties encountered in traditional breeding methods, the potential from molecular breeding in improving forage crops has been recognized. Plant engineering strategies for abiotic stress tolerance largely rely on the gene expression for enzymes involved in pathways leading to the synthesis of functional and structural metabolites, proteins that confer stress tolerance, or proteins in signaling and regulatory pathways. Genetic engineering allows researchers to control timing, tissue-specificity, and expression level for optimal function of the introduced genes. Thus, the use of either a constitutive or stress-inducible promoter may be useful in certain cases. In this review, we summarize the recent progress made towards the development of transgenic forage plants with improved tolerance to abiotic stresses.

재생디젤기관차 주강대차의 안전성 평가에 관한 연구 (A Study on the Safety Evaluation of Cast-iron Bogie of Rebuiled Diesel-Electric Locomotive)

  • 이찬우;서정원
    • 한국철도학회:학술대회논문집
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    • 한국철도학회 2001년도 추계학술대회 논문집
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    • pp.240-246
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    • 2001
  • The main objective of the study is to provide the criteria for determining the proper life cycle of rebuiled diesel-electric locomotive currently being operated in KNR. We predicted the most critical sections of the cast-iron bogie and tested the diesel-electric locomotive vehicle to measure dynamic stresses applied to the bogie. The fileld test of the diesel-electric locomotive estimated 9.90years based on the fatigue-life estimation when they are continuously used in the present operation condition.

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일정스트레스 가속수명시험의 경제적 설계 (An Economic Design of Constant Stress Accelerated Life Tests)

  • 윤원영;반한석
    • 한국경영과학회지
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    • 제19권1호
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    • pp.145-152
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    • 1994
  • This paper deals with an economic design of acelerated life test under constant stresses where failure times are exponentially distributed. In this case the optimization criterion is the information amount per unit cost. Fisher's information matrix of exponential distribution's parameters and expected cost considering fixed and variable costs are obtained. The decision variable is the censoring time in the model. In the 2-level constant stress case, it is proved that the optimal solution exists and is unique under some condition. Numerical examples are also included.

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자동차 환경을 고려한 커넥터의 가속시험에 관한 연구 (Highly Accelerated Life Tests for Auto-Connector in Use-Environment)

  • 김종걸;김진환
    • 대한안전경영과학회:학술대회논문집
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    • 대한안전경영과학회 2004년도 추계학술대회
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    • pp.229-235
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    • 2004
  • This paper treats accelerated life tests for automotive connector. The contact resistance of connector is explained by some factors; the use time(calender time, real cycle), stresses and loads adapted in auto test. The relationships between contact resistance and some factors are compared and analyzed by regression models in various test conditions; field use-environment, manufacturer's test environment, and accelerated test condition. The consistency between of manufacturer's test and field test is examined. Finally, the future study on accelerated test for automotive connector is presented.

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두 가지 항산화유전자를 동시에 발현시킨 형질전환 톨 페스큐 식물체의 환경스트레스에 대한 내성 특성 해명 (Characterization of Transgenic Tall Fescue Plants Expressing Two Antioxidant Genes in Response to Environmental Stresses)

  • 이상훈;이기원;김기용;최기준;서성;곽상수;권석윤;윤대진;이병현
    • 한국초지조사료학회지
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    • 제27권2호
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    • pp.109-116
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    • 2007
  • 환경스트레스에 강한 내성을 지닌 신품종 톨페스큐를 개발할 목적으로 산화스트레스에 의해 강하게 유도되는 SWPA2 promoter 하류에 CuZnSOD와 APX 유전자가 엽록체에 동시에 발현하도록 제작한 벡터를 Agrobacterium법을 이용하여 톨 페스큐에 도입하였다. Hygromycin이 첨가된 선발배지에서 내성을 가지며 재분화된 형질전환 식물체를 pot로 이식하여 기내 순화시킨 후, Southern 분석을 실시하여 본 결과, 발현벡터의 T-DNA 영역이 형질전환 식물체의 genome에 성공적으로 도입되었음을 확인하였다. 형질전환 식물체 잎 절편을 산화스트레스와 중금속을 포함하고 있는 용액에 처리하여 엽록체의 손상정도를 조사한 결과, 비형질 전환체에 비해 형질전환체는 강한 내성을 나타내었다. 또한 유식물체 수준에서 MV를 처리하여 내성을 비교한 결과, 비형질전환체에 비해 형질전환체는 손상을 덜 받았다. 이와 같은 연구결과는 CuZnSOD와 APX 유전자를 엽록체에 동시발현시키는 기술이 다양한 환경스트레스에 대해 복합재해내성을 가지는 다양한 작물을 개발하는데 유용하게 이용될 수 있음을 나타낸 결과이다.

피로강도 및 수명에 미치는 Shot Peening에 의한 잔류응력의 영향 (Influence of residual stress due to shot peening on fatigue strength and life)

  • 이종규;김정규
    • 대한기계학회논문집A
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    • 제21권9호
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    • pp.1498-1506
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    • 1997
  • Procedures are presented for influence of shot peening on fatigue strength, fatigue life and effects of shot peening are discussed from experiments were taken between shot peened and unpeened SPS5, SM45C specimens. After the residual stress on shot peened specimens was measured by X-ray diffractometer, rotating bending fatigue tests were carried out. In addition, the compressive residual stress profile was obtained by the superposition method of three stresses which is based on Al-Obaid's equation. Predicted fatigue life considering residual stress profile which was obtained by the Al-Obaid's equation and another predicted fatigue life considering residual stress profile which was measured in test were compared. For the purpose of predicting fatigue life, Morrow's equation considering the residual stress and mean stress was used.

길들이기 과정을 고려한 변속기용 기어의 접촉피로 수명 해석 (The Contact Fatigue Life Analysis of Transmission Gear considering Running-in)

  • 문길환;이상돈;조용주
    • 한국자동차공학회논문집
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    • 제15권5호
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    • pp.133-138
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    • 2007
  • Surface pitting is a major failure mode for gears. The contact fatigue life analysis of transmission gear considering running-in process is presented in this paper. Surface roughness change of rolling test is used in a life analysis. Contact stresses are obtained by contact analysis of a semi-infinite solid based on the use of influence functions; the subsurface stress field is obtained using rectangular patch solutions. Mesoscopic multiaxial fatigue criterion which can yield satisfactory results for non-proportional loading is then applied to predict fatigue damage. Suitable counting method and damage rule were used to calculate the fatigue life of random loading caused by rough surface. The life analysis considering running-in is in good agreement with the experimental results.