• 한국육종학회지
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• 제40권1호
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• pp.1-7
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• 2008

An attempt was made to link rice embryo proteins to DNA sequences and to understand their functions. One hundred of the 700 spots detected on the embryo 2-DE gels were microsequenced. Of these, 28% of the embryo proteins were matched to DNA sequences with known functions, but 72% of the proteins were unknown in functions as previously reported (Woo et al. 2002). In addition, twenty-four protein spots with 100% of homology and nine with over 80% were matched to ESTs (expressed sequence tags) after expanding the amino acid sequences of the protein spots by Database searches using the available rice EST databases at the NCBI (http://www/ncbi.nlm.nih.gov/) and DDBJ (http://www.ddbj.nig.ac.jp/). The chromosomal location of some proteins were also obtained from the rice genetic map provided by Japanese Rice Genome Research Program (http://rgp.dna.affrc.go.jp). The DNA sequence databases including EST have been reported for rice (Oryza sativa L.) now provides whole or partial gene sequence, and recent advances in protein characterization allow the linking proteins to DNA sequences in the functional analysis. This work shows that proteome analysis could be a useful tool strategy to link sequence information and to functional genomics.

• Journal of Microbiology
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• 제45권2호
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• pp.153-157
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• 2007

5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase, EC 2.5.1.19) is the sixth enzyme in the shikimate pathway which is essential for the synthesis of aromatic amino acids and many secondary metabolites. The enzyme is widely involved in glyphosate tolerant transgenic plants because it is the primary target of the nonselective herbicide glyphosate. In this study, the Dunaliella salina EPSP synthase gene was cloned by RT-PCR approach. It contains an open reading frame encoding a protein of 514 amino acids with a calculated molecular weight of 54.6 KDa. The derived amino acid sequence showed high homology with other EPSP synthases. The Dunaliella salina EPSP synthase gene was expressed in Escherichia coli and the recombinant EPSP synthase were identified by functional complementation assay.

• International Journal of Industrial Entomology and Biomaterials
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• 제18권2호
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• pp.125-129
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• 2009

Entomopathogenic fungi were isolated directly from a cadaver of adult Monochamus alternatus supporting fungal sporulation, using a semi-selective medium and then screened several fungal colonies. The pathogenicity of each fungus was tested using oak longicorn beetle, Moechotypa diphysis, as substitutive insect. As the result, only one of them showed high pathogenicity against M. diphysis, with up to 100% mortality within 21 days of inoculation. Selected fungus was named as MaW1 and identified by Beauveria bassiana using microscopic examination and DNA analysis. Pathogenicity was also evaluated to M. alternatus.

• 한국보육지원학회지
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• 제8권2호
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• pp.157-174
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• 2012

본 연구는 어린이집에 근무하고 있는 교사의 삶의 만족도에 어떤 요인들이 영향을 미치는 지를 탐색하고자 시도하였다. 이를 위해 전국의 어린이집에 근무하고 있는 여자 교사 302명을 대상으로 삶의 만족도에 영향을 주는 요인들 즉 근무환경요인(사회지지와 근무환경), 업무환경요인(업무스트레스와 직무만족도) 및 소진요인으로 범주화하여 이들 변인이 어린이집 교사의 삶의 만족도에 미치는 영향을 분석하였다. 분석결과 첫째, 업무스트레스에 직접적인 영향을 미치는 요인으로는 사회지지와 근무환경이었다. 둘째, 직무만족도에 직접적인 영향을 미친 요인으로는 사회지지와 업무스트레스였다. 셋째, 소진에 직접적인 영향을 미치는 요인으로는 사회지지와 업무스트레스, 직무만족도였다. 넷째, 삶의 만족도에 직접적인 영향을 미친 요인으로는 근무환경과 직무만족도 및 소진이었다. 본 연구결과를 종합하면 어린이집 교사의 삶의 만족도에 근무환경, 직무만족도 및 소진은 직접적으로 영향을 미치고 있으며, 사회지지와 업무스트레스는 소진을 통해 간접적으로 영향을 미치고 있는 것으로 나타났다.

• The Plant Pathology Journal
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• 제17권6호
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• pp.361.1-361
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• 2001

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.325-325
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• 2005

• 한국작물학회지
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• 제68권2호
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• pp.97-97
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• 2023

• 한국응용곤충학회:학술대회논문집
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• 한국응용곤충학회 2003년도 추계 학술발표회
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• pp.158-158
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• 2003

• 한국물환경학회지
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• 제20권3호
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• pp.206-214
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• 2004

Residual wall thicknesses, corrosion rates, and residual life of thirty four samples of cast iron pipes(CIPs) and ductile cast iron pipes(DCIPs) collected from water mains of B city were studied to estimate their remaining service life or optimum time of rehabilitation. The internal maximum corrosion depths of samples measured using a dial gauge after shot blasting were twice higher than the external in most cases. Therefore corrosion of water pipes was much more affected by internal water quality than soil. Residual wall thicknesses of DCIPs were higher than those of CIPs. That reason was thought to be that DCIPs have been protected from internal corrosion by lining cement mortar. Residual life calculated by maximum corrosion rate was ranged up to 44 years with 12.40 years average. Since most CIPs were much deteriorated, rehabilitation plan should be established soon in B city. Residual life of DCIP was 33.52 years average. When cement mortar lining is used up by neutralization of DCIPs. DCIP also should be rehabilitated.

• 한국작물학회지
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• 제51권6호
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• pp.544-553
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• 2006

In order to assess antioxidant capacity in relation to seed traits of rice (Oryza sativa L.), ninety-six varieties were examined for antioxidative activity of brown rice grain using superoxide dismutase (SOD), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and thiobarbituric acid (TBA) assays. Overall, average total activities measured by the three methods were of very wide range between 64% and 13%. Significant differences were noted depending on the variety and evaluation method. Rice varieties with foreign origin, middle maturity, colored hulls, and colorless awn exhibited statistically significant higher total activity. As for the measurements, total activity was significantly correlated with SOD (r=0.29***), DPPH (r=0.80***) and TBA (r=0.76***). Between the three activities, SOD was not positively correlated with DPPH (r=0.15*), while TBA was significantly correlated with DPPH value (r=0.51***). DPPH (55.20%) and TBA (50.36%) were significantly higher in foreign rice, while SOD activity (44.29%) was significantly higher in domestic rice. However, an average total activity was significantly higher in foreign rice (47.31%) than in domestic rice (35.92%). SOD, DPPH and TBA activities of middle maturity in maturity time were the highest total activity (44.96%) and significantly differed from the other two groups. Total activity was significantly higher in rice with a colorless awn (42.18%) than with a colored awn (35.87%).