• Journal of Life Science
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• v.13 no.1
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• pp.128-135
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• 2003

102 ESTs (Expressed Sequence Tags) were obtained by sequencing clones from a library of rainbow trout kidney cDNAs. Of the sequences generated, 55.8% of the ESTs were represented by 37 known genes. The 45 clones of unknown gene products potentially represent 40 novel genes. The genes involved in structural function (14.5%) and transcription/translation (11.6%) account for the major gene expression activities in the kidney Microarray experiment was conducted to compare gene expression of the unique ESTs in young and adult rainbow trout kidneys. While mitochondrion, cytochrome b, rho G, spastin protein, and three unknown genes were down-regulated in the mature fish kidney, calponin 1, calcium binding protein, histone deacetylase 1, and an unknown gene were up-regulated in the mature fish kidney. This research demonstrates the feasibility and power of functional genomics in rainbow trout.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.45 no.4
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• pp.94-104
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• 2008

In this paper, we propose a new architecture of multiplier-and-accumulator (MAC) for high speed multiplication and accumulation arithmetic. By combining multiplication with accumulation and devising a hybrid type of carry save adder (CSA), the performance was improved. Since the accumulator which has the largest delay in MAC was removed and its function was included into CSA, the overall performance becomes to be elevated. The proposed CSA tree uses 1's complement-based radix-2 modified booth algorithm (MBA) and has the modified array for the sign extension in order to increase the bit density of operands. The CSA propagates the carries by the least significant bits of the partial products and generates the least significant bits in advance for decreasing the number of the input bits of the final adder. Also, the proposed MAC accumulates the intermediate results in the type of sum and carry bits not the output of the final adder for improving the performance by optimizing the efficiency of pipeline scheme. The proposed architecture was synthesized with $250{\mu}m,\;180{\mu}m,\;130{\mu}m$ and 90nm standard CMOS library after designing it. We analyzed the results such as hardware resource, delay, and pipeline which are based on the theoretical and experimental estimation. We used Sakurai's alpha power low for the delay modeling. The proposed MAC has the superior properties to the standard design in many ways and its performance is twice as much than the previous research in the similar clock frequency.

• Korean Journal of Microbiology
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• v.49 no.1
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• pp.51-57
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• 2013

As facilities and equipments for learning activities in college campuses are handled by mass public, their contact surfaces may function as major routes of cross-infection of microbial pathogens. However, unlike items in cafeteria which is the typical target for campus hygiene, those surfaces are not under regular surveillance or sanitary maintenance. In this study, I made a quantitative comparison of the risk of being exposed to microbial pathogens from use of learning facilities such as classrooms and library to the risk from use of cafeteria, for about 1,500 students in a college. Regarding total coliforms as surrogate model of bacterial pathogens, exposure rates were estimated for each item in learning facilities and cafeterias by devising deterministic exposure algorithms based on bacterial abundance, contract rates and transfer rates. The exposure rate in cafeterias was 1.0 CFU/day while learning facilities imposed the rate of 0.5 CFU/day, which reaches a half of the exposure rate in cafeterias. However, 70% of students were exposed more in learning facilities than cafeteria because individuals had different frequencies in using cafeteria. Based on the results, some human-contact surfaces of learning facilities, including elevator buttons, may require regular sanitary maintenance. An efficient sanitary maintenance considering seasonality in diversity of pathogens involved with cross-infections is suggested besides improvement of personal hygiene among students.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.3
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• pp.32-39
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• 2018

Raman spectra exhibit differences in intensity depending on the measuring equipment and environmental conditions even for the same material. This restricts the pattern recognition approach of Raman spectroscopy and is an issue that must be solved for the sake of its practical application, so as to enable the reusability of the Raman database and interoperability between Raman devices. To this end, previous studies assumed the existence of a transfer function between the measurement devices to obtain a direct spectral correction. However, this method cannot cope with other conditions that cause various intensity distortions. Therefore, we propose a classification method using linear intensity calibration which can deal with various measurement conditions more flexibly. In order to evaluate the performance of the proposed method, a Raman library containing 14033 chemical substances was used for identification. Ten kinds of chemical Raman spectra measured using three different Raman spectroscopes were used as the experimental data. The experimental results show that the proposed method achieves 100% discrimination performance against the intensity-distorted spectra and shows a high correlation score for the identified material, thus making it a useful tool for the identification of chemical substances.

• Journal of the Korean Society of Hazard Mitigation
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• v.10 no.3
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• pp.61-69
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• 2010

Disasters have been diversifying and the scale of disaster has been increasing worldwide due to the global warming and urbanization. Consequently, it is essential to establish the systematic disaster management because the scale of damages has been rapidly increasing. Nowadays, the importance of the standardized disaster management have been realized internationally due to the 9.11 terror and Tsunami. Also, the activities of research and development to utilize and establish the disaster management standards have been increasing. This study mainly focused on generating an efficient operating manual to support the systematic disaster management of the central administration office based on disaster management standard in South Korea. Firstly, the activities and status of disaster management performed by the central administration office were investigated. Accordingly, libraries of work, functions, organizations, references and behavior for disaster management were designed. Then, a method to make the efficient operation manual based on the constructed libraries was presented to maximize the efficiency of disaster management. This emergency operation manual could support the systematic disaster management by defining the work, function, references and the codes of conduct. Thus, central administration office would be able to define methods and procedures from preparation to recovery through the utilization of the operation manual.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.1
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• pp.46-54
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• 1997

Sporulation in the fission yeast Schizosaccharomyces pombe has been regarded as an important model of cellular development and differentiation. S. pombe cells proliferate by mitosis and binary fission on growth medium. Deprivation of nutrients especially nitrogen sources, causes the cessation of mitosis and initiates sexual reproduction by malting between two sexually compatible cell types. Meiosis is then followed in a diploid cell in the absence of nitrogen source. DNA fragment complemented with the mutations of sporulation gene was isolated from the S. pombe gene library constructed in the vector, pDB 248' and designated as pDB (spo 5)1. We futher analyzed six recombinant plasmids, pDB (spo 5)2, pDB(spo 5)3, pDB(spo 5)4, pDB(spo 5)5, pDB(spo 5)6, pDB(spo 5)7, and found each plasmids is able to rescue the spo 5-2, spo 5-3, spo 5-4, spo 5-5, spo 5-6, spo 5-7, mutations, respectively. Mapping of the integrated plasmid into the homologous site of the S. pombe chromosomes demonstrated that pDB (spo 5)1, and pDB (spo 5)R1 contained the spo 5 gene. Transcipts of spo 5 gene were analyzed by Northern hybridization. Two transcripts of 3.2 kb and 25 kb were detected with 5 kb Hind III fragment containing a part of the spo 5 gene as a probe. The small mRNA (2.5 kb) appeared only when a wild-type strain was cultured in the absence of nitrogen source in which condition the large mRNA (3.2 kb) was produced constitutively. Appearance of a 2.5 kb spo 5-mRNA depends upon the function of the mei1, mei2 and mei3 genes.

• Journal of Korean Society of Transportation
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• v.18 no.4
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• pp.7-18
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• 2000

KS-SIGNAL, a traffic signal optimization model for coordinated arterials, is an optimization model using the mixed integer linear Programming that minimizes total delay on arterials by optimizing left-turn Phase sequences. However, the Previous version of KS-SIGNAL had a difficulty in reducing computation speed because the related variables and constraints multiply rapidly in accordance with the increase of intersections. This study is designed to propose a new model, improving optimizing computation speed in KS-SIGMAl, and evaluate it. This Paper Puts forth three kinds of methodological approaches as to achieve the above goals. At the first step to reduce run-time in the proposed model objective function and a few constraints are Partially modified, which replaces variable in related to queue clearance time with constant, by using thru-movements at upstream intersection and the length of red time at downstream intersection. The result shows that the run-time can be reduced up to 70% at this step. The second step to load the library in LINDO for Windows, in order to solve mixed integer linear programming. The result suggests that run-time can be reduced obviously up to 99% of the first step result. The third step is to add constraints in related to left-turn Phase sequences. The proposed methodological approach, not optimizing all kinds of left-turn sequences, is more reasonable than that of previous model , only in the view of reducing run-tim. In conclusion, run-time could be reduced up to 30% compared with the second results. This Proposed model was tested by several optimization scenarios. The results in this study reveals that signal timing plan in KS-SIGNAL is closer to PASSER-II (bandwidth maximizing model) rather than to TRANSYT-7F(delay minimizing model).

• Journal of the Korea Society of Computer and Information
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• v.11 no.6 s.44
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• pp.69-78
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• 2006

In the field of the bioinformatics, it plays an important role in predicting functional information or structure information to search similar sequence in biological DB. Biolrgical sequences have been increased dramatically since Human Genome Project. At this point, because the searching speed for the similar sequence is highly regarded as the important factor for predicting function or structure, the SMP(Sysmmetric Multi-Processors) computer or cluster is being used in order to improve the performance of searching time. As the method to improve the searching time of BLAST(Basic Local Alighment Search Tool) being used for the similarity sequence search, We suggest the nBLAST algorithm performing on the cluster environment in this paper. As the nBLAST uses the MPI(Message Passing Interface), the parallel library without modifying the existing BLAST source code, to distribute the query to each node and make it performed in parallel, it is possible to easily make BLAST parallel without complicated procedures such as the configuration. In addition, with the experiment performing the nBLAST in the 28 nodes of LINUX cluster, the enhanced performance according to the increase in the number of the nodes has been confirmed.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.1A
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• pp.93-98
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• 2004

Serial ATA interface Is inexpensive comparatively and performance is superior. So it is suitable technology in demand that now require data transmission and throughput of high speed. This paper describes a design and implementation of Serial ATA Link layer about error detection and 8b/10b encoder/decoder for DC balance in frequency 150MHz. The 8b/10b Encoder is partitioned into a 5b/6b plus a 3b/4b coder. The logical model of the block is described by using Verilog HDL at register transistor level and the verified HDL is synthesized using standard cell libraries. And it is fabricated with $0.35{\mu}m$ Standard CMOS Cell library and the chip size is about $1500{\mu}m\;*\;1500{\mu}m$. The function of this chip has been verified and tested using testboard with FPGA equipment and IDEC ATS2 test equipment. It is used to frequency of 100MHz in verification processes and supply voltage 3.3V. The result of testing is well on the system clock 100MHz. The designed and verified each blocks may be used IP in the field of high speed serial data communication.

• Journal of Life Science
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• v.18 no.9
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• pp.1244-1248
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• 2008

P-glycoprotein (P-gp) is a very important drug transporter, which plays an important role in drug disposition and represents an additional mechanism for the development of multidrug resistance. Flavonoids, a major class of natural compounds widely present in foods and herbal products, have been shown to be P-gp inhibitors. The objective of the present study was to identify new chemosensitizer candidates through the screening of various herbal extracts. The inhibitory effects of herbal extracts on P-gp activity were assessed by measuring accumulation of calcein AM using P-gp overexpressed L-MDR1 cells. Curcuma longa showed the most potent inhibition on P-gp function. The inhibitory potential of P-gp was in the order: Curcuma longa > Curcuma aromatica > Ageratum conizoids > Zanthoxylum planispinum > Zedoariae rhizome > Rakta chandan > Dalbergia odorifera > Caesalpinia Sappan > Aloe ferox. To identify individual constituents with inhibitory activity, the herbal extracts were analyzed by LC/MS/MS. Several flavonoids such as curcumin, a well-known P-gp inhibitor, were identified through mass spectral library search. These in vitro data indicate that herbal extracts contain constituents that can potently inhibit the activities of P-gp and suggest that these herbal extracts should be examined for potential chemosensitizer in vivo.