• 말소리와 음성과학
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• 제6권4호
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• pp.161-169
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• 2014

This study investigated changes in the prelinguistic vocal production of typically developing infants aged 5-20 months based on Stark Assessment of Early Vocal Development-Revised (SAEVD-R). Fifty-eight typically developing infants participated in the study, and they were divided into four age groups, 5-8 months, 9-12 months, 13-16 months, and 17-20 months of age. Vocalization samples were collected from infants' play activities and were classified into 5 levels and 23 types using SAEVD-R. The results revealed that the four age groups showed significant differences in production proportion of vocalization levels. Level 1, 2, 4, and 5 vocalizations exhibited significantly different across the four age groups. Level 3 was predominantly produced across every age group. Therefore, the vocalization level was not significantly different across the four age groups. Especially, vowels in Level 3 vocalization predominantly produced across all ages during a long period. Also, significant increases in the proportion of Levels 4 and 5 occurred after 9 months, which suggested that the production of cannonical syllables is a key indicator of advancement in prelinguistic vocal development. The results have clinical implication in early identification and speech-language intervention for young children with speech delays or at risk.

• Molecules and Cells
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• 제26권1호
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• pp.18-25
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• 2008

Arsenic trioxide (ATO) can affect many biological functions such as apoptosis and differentiation in various cells. We investigated the involvement of ROS and GSH in ATO-induced HeLa cell death using ROS scavengers, especially N-acetylcysteine (NAC). ATO increased intracellular ${O_2}^{{\cdot}-}$ levels and reduced intracellular GSH content. The ROS scavengers, Tempol, Tiron and Trimetazidine, did not significantly reduce levels of ROS or GSH depletion in ATO-treated HeLa cells. Nor did they reduce the apoptosis induced by ATO. In contrast, treatment with NAC reduced ROS levels and GSH depletion in the ATO-treated HeLa cells and prevented ATO-induced apoptosis. Treatment with exogenous SOD and catalase reduced the depletion of GSH content in ATO-treated cells. Catalase strongly protected the cells from ATO-induced apoptosis. In addition, treatment with SOD, catalase and NAC slightly inhibited the G1 phase accumulation induced by ATO. In conclusion, NAC protects HeLa cells from apoptosis induced by ATO by up-regulating intracellular GSH content and partially reducing the production of ${O_2}^{{\cdot}-}$.

• 한국환경보건학회지
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• 제45권6호
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• pp.630-637
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• 2019

Objectives: This study was performed to evaluate the secular changes in indoor airborne dust or endotoxin levels in the dust from swine confinement buildings. Indoor levels were compared with the level at the exhaust outlet in order to examine the contribution potential of indoor dust to nearby ambient air dust. Methods: Comparisons were made on inhalable and respirable dust levels reported in 2002, 2012, and 2017 from 14, 10, and 36 swine fattening confinement buildings in Korea, respectively. This data was produced by the same research group. Levels of endotoxin adsorbed into inhalable or respirable dust were also compared. Samples of inhalable or respirable dust were collected indoors and at exhaust outlets from 17 swine fattening confinement buildings in 2019, and dust levels were compared between the indoor and the outlet. Results: The outlet inhalable dust level (0.111 mg/㎥) was approximately 19% of that from indoors, and the respirable dust level (0.033 mg/㎥) was approximately 74% of that from indoors. The outlet respirable dust levels were lower than the airborne fine dust levels in the towns where those farms are located. No significant difference was observed in the inhalable dust levels among the years examined, but the respirable dust level in 2017 (0.143 mg/㎥) was significantly lower than in 2002 (0.328 mg/㎥). The level of endotoxin in inhalable dust was significantly higher in 2017 (722 EU/㎥) than in both 2002 (75 EU/㎥) and 2012 (171 EU/㎥). Conclusion: Even though no apparent contribution from swine farm indoor dust to nearby ambient air dust was observed in terms of amount, a certain control strategy to reduce the production of airborne dust and endotoxin from swine farms is merited.

• Plant Breeding and Biotechnology
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• 제2권3호
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• pp.289-300
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• 2014

A tissue-specific and developmentally expressed gene was isolated from Chinese cabbage (Brassica rapa L. ssp. pekinensis), designated BrREF (B. rapa Rubber elongation factor). BrREF transcripts were expressed at high levels in seedlings and at low levels in flower buds and roots. To study the activity of this promoter, the 2.2 kb upstream sequence of BrREF gene was fused to a β-glucuronidase (GUS) reporter gene and was introduced into Arabidopsis thaliana and B. napus by Agrobacterium-mediated transformation. Strong expression of GUS driven by the BrREF promoter was detected in the cotyledons and hypocotyls of transgenic plant seedlings, but GUS expression was weak in roots, excluding the root tips. GUS expression in the cotyledons and hypocotyls decreased dramatically as the seedlings matured and was not detected in the tissues of mature plants. During floral development, GUS expression was observed in immature anthers. These findings suggest that the BrREF promoter can modulate the tissue-specific and developmental expression of gene at the early stages of growth and development.

• 가정과삶의질연구
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• 제25권2호
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• pp.47-58
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• 2007

The purpose of this study is to examine children's/adolescents' motives for playing internet games and adaptation to school in accordance with their frequency levels of playing internet games. Participants were 236 children from grades 4, 5, and 6 in two elementary schools and 237 adolescents from grades 1, 2, and 3 in one middle school. They were measured on frequency levels of playing internet games, motives for playing internet games, and adaptation to school. Collected data were analyzed by SPSS WIN 12.0 for reliability, frequency, Chi-square test, t-test, Pearson's correlation, and one way ANOVA. Major findings of the research are as follows: First, children's/adolescents' frequency levels of playing internet games differed by their gender. The male ratio was much higher than the female ratio in the potential risk group and the high risk poop. In contrast, no grade differences on the frequency levels of playing internet games were found. Second, children's/adolescents' motives for playing internet games differed by their gender. Especially, among the motives the greatest difference between male and female was marked for 'Social Relationship'. Third, children's/adolescents' motives for playing internet games differed by their frequency levels of playing internet games. For 'Conformity,' the low risk group differed from the high risk group. For 'Spending Time', 'Benefit', 'Competition', and 'Social Relationship', the low risk group differed from the potential risk group and also from the high risk poop. Fourth, children's/adolescents' adaptation to school differed by their frequency levels of playing internet games. The high risk group exhibited a low level of adaptation in 'Academic Activities', 'a subcategory of adaptation to school.

• 한국가축번식학회지
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• 제20권3호
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• pp.353-360
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• 1996

The study was conducted to determine the optimal cumulus cells, glucose and superoxide dimutase(SOD) levels during the in vitro culture of bovine oocytes matured and fertilized in vitro for morulae and blastocyst development. Oocytes were cultured for 0~8 days in TCM-199 medium supplemented with 20% FCS, cumulus cells and with different glucose and SOD levels. The results are summarized as follows; 1. The in vitro developmental rates of bovine oocytes cultured in TCM-199 medium containing cumulus cells and 0.1, 0.5, 1.0, 5.0mM glucose levels 0~3 and 0~8 days after insemination were 21.1, 25.0, 23.3, 17.9 and 26.3, 25.7, 23.1, 19.4% respectively and there was significant differences on the development to the molurae and blastocysts stage among the cumulus cells and glucose levels. 2. The in vitro developmental rates of bovine oocytes cultured in TCM-199 medium containing 0.1, 0.5, 1.0, 5.0mM glucose levels 0~3 and 0~8 days after insemination were 11.3~24.5% and 17.3~25.0%, respectively. 3. The in vitro developmental rates bovine oocytes cultured in TCM-199 medium containing 100, 200, 300, 500 $\mu\textrm{g}$/ml SOD levels 0~3 and 0~8 days after insemination were 12.5~22.9% and 12.9~22.2%, respectively. Hight levels of SOD(500$\mu\textrm{g}$/ml) significantly reduced the rates ofmolurae and blastocysts stage(P<0.05).

• 동물자원연구
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• 제28권2호
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• pp.56-63
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• 2017

본 연구는 고온기 때 사료 내 다른 에너지 수준 및 비테인 첨가 급여가 육성돈의 영양소 소화율 및 생리학적 변화에 미치는 영향을 구명하기 위해 실시하였다. 실험동물은 삼원교잡종($L{\times}Y{\times}D$; initial body weight, $73.5{\pm}0.5kg$) 거세 수퇘지 12두를 사용하였고 대사틀에 배치하였다. 실험기간은 고온기인 7~8월에 실시하였다. 실험계획은 에너지 2수준(3,300 및 3,400kcal/kg)과 비테인 2수준(0 및 0.5%)이며 $4{\times}4$ Latin square로 하였다. 조단백질 소화율은 고에너지 사료(3,400kcal/kg)가 저에너지 사료보다 유의적으로 높았다(p<0.01). 그러나, 비테인급여는 영양소소화율에 영향을 미치지 않았다. 혈액생화학적 분석 결과에서는 에너지 수준 및 비테인 첨가가 육성돈 내 생리적 변화를 보이지 않았다. 면역반응을 나타내는 혈중 IgG에서는 고에너지 사료가 저에너지사료보다 높았으나(p<0.05) 스트레스 지표를 나타내는 cortisol농도에서는 차이가 나지 않았고, 비테인 첨가급여는 IgG 및 cortisol 농도 변화를 나타내지 않았다. 결론적으로 사료 내 비테인 첨가급여보다 에너지 수준을 높이는 것이 돼지 체내에 더 긍정적인 효과를 보이며, 여름철 고온스트레스를 받는 돼지 사료 내 고에너지를 급여했을 때 어떠한 결과가 나오는지 추후 더 연구해 볼 만한 것으로 사료된다.

• 한국수산과학회지
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• 제40권5호
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• pp.269-275
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• 2007

The toxicity of two species of puffer fish, Takifugu pardalis and T. niphobles, collected from the coastal regions of Korea was determined using a mouse bioassay. In T. pardalis collected at Tongyeong, the proportion of toxic specimens containing ${\geq}10MU/g$ exceeded 90% for the skin, fins, liver, intestine, ovary, and gallbladder, 11.1% for the testis, and 6.9% for the muscle. In each of the organs, the highest toxin levels were several tens (14-39) of mouse units (MU) per gram in the muscle, testis, and eyeball, but thousands (1,444-5,755) of MU per gram in the skin, liver, intestine, ovary, and gallbladder. The organs of T. pardalis exhibited remarkable variation in toxicity. In T. niphobles, the proportion of toxic specimens exceeded 90% for the ovary and skin, 60-80% for the fins, liver, intestine, and gallbladder, and 4.5% for the muscle; no toxicity was detected in the testis or eyeball using the mouse bioassay. The highest toxin levels were thousands (2,291-7,777) of MU per gram in the liver, intestine, ovary, and gallbladder, hundreds(146-328) of MU per gram in the skin and fins, and 18 MU/g in the muscle. Takifugu niphobles toxicity also exhibited remarkable regional variation. The toxicity in the edible muscle of T. pardalis and T. niphobles was at acceptable levels for human consumption, while the toxicity of the skin of both species of puffer fish was very high, so that care must be taken when used for human consumption.

• Fisheries and Aquatic Sciences
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• 제7권4호
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• pp.184-191
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• 2004

A 25-week feeding trial of two dietary protein (47 and $52\%$) and three dietary lipid level (7, 12 and $17\%$) factorial design with three replications were conducted to determine effects of dietary protein and lipid levels on growth, feed utilization and body composition of adult starry flounder (Platichthys stellatus), average initial weight 332 g, during the winter season. Survival of fish was not affected by either dietary protein or dietary lipid level. Weight gain, feed efficiency and protein efficiency ratio improved with dietary protein and lipid levels except for those of fish fed the $52\%$ protein diet with $17\%$ lipid. The best growth and feed utilization were observed in the $52\%$ protein diet with $12\%$ lipid, but were not significantly different from those of fish fed the $52\%$ protein diet with $17\%$ lipid or the $47\%$ protein diets with $17\%$ lipid levels. Hepatosomatic and visceral somatic indexes were significantly influenced by dietary protein level, but not by dietary lipid level. None of moisture, crude protein, crude lipid, or glycogen contents of dorsal muscle or liver in starry flounder except for crude lipid in dorsal muscle was significantly influenced by either dietary protein or dietary lipid level. Plasma cholesterol concentration was significantly influenced by both dietary protein and dietary lipid levels. The results of this study suggest that the diets containing $47\%$ protein with $17\%$ lipid or $52\%$ protein with $12-17\%$ lipid are optimal for growth and feed utilization of adult starry flounder under these experimental conditions.

• Journal of Nutrition and Health
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• 제9권4호
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• pp.11-18
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• 1976

In this third report influence of vibration on growth and on some metabolism of young growing rats fed on varying levels of protein was investigated. Forty eight (48) young growing male rats weighing about 60 grams were used, grouping to four (4) groups, twelve (12) rats each group. They were fed on 8%, 13%, 19%, and 26% casein diet respectively (See the table 1) for the period of 10 weeks experiment. During the experimental period the half number of the rats of each group were subjected to a given degree of vibration for two (2) hours daily. Observations for growth rate, food and protein efficiency ratios, organs development, cholesterol levels in aorta, total nitrogen, urea nitrogen and creatinine levels in urine may be summarized as follows 1. Growth was impaired by the vibration in all groups including 26% easein diet. 2. There is tendeney that in higher protein diets, the organs (See table 3) developed more well. And also the impairment of the organs development by the vibration revealed less in higher protein diets. 3. Food and protein efficiency ratios were generally decreased under vibration and the food efficiency was improved by increasing the protein level in diet. 4. Total and free cholesteral levels in aorta were increased by the vibration. Ester from/tatal ratio was 17.7% and 17.3% respectively at 8% and 13% protein diets and 54.8% and 54.2% at 19% and 26% protein diets. These show that, in higher protein diets, the vibration doesn't influence the cholesterol ratio. 5. Total nitrogen, urea nirogen and creatinine levels in urine were increased by increasing the protion level in diet and also increased by the vibration. 6. It seems that, according to the above observations, the vibration used in this experiment influenced, in certain extent, on physical development, physiological availability of nutrients, and on body metabolism. And it is also thought that higher protein diets act some good role in protecting body from suffering from vibration.