• 한국어병학회지
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• 제31권2호
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• pp.109-113
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• 2018

The purpose of the present study was to determine whether the scuticocidal activity of olive flounder (Paralichthys olivaceus) head-kidney leucocytes can be enhanced by stimulation with polyinosine-polycytosine [poly (I:C)]. The growth of Miamiensis avidus was not affected by exposure to unstimulated or poly (I:C)-stimulated leucocytes alone, heat-inactivated immune serum alone, or unstimulated leucocytes plus heat-inactivated immune serum. However, leucocytes stimulated with poly (I:C) showed clearly high scuticocidal activity against M. avidus in the presence of heat-inactivated immune serum. Furthermore, numerous poly (I:C)-stimulated leucocytes occupied the surface of scuticociliates in the presence of the heat-inactivated immune serum, which led to lysis of scuticociliates. These results suggest that both of the stimulation of leukocytes and the immobilization of scuticociliates are necessary to kill scuticociliates by leukocytes.

• 한국동물위생학회지
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• 제22권4호
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• pp.377-383
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• 1999

Polymorphonuclear (PMN) leucocytes are fundamental importance to the body's defense mechanism and play a major role in the local and systemic reactions to infectious disease. Investigation of the physiological and pathological role of the various leucocyte subtypes in host defence mechanisms is dependent upon the isolation of adequate numbers of viable, pure leucocyte fractions. This report describes the separate frequency of PMN leucocytes both from buffy coat layer and from packed RBC layer when bovine peripheral blood was treated with various anti-coagulants such as acid-citrate-dextrose(ACD), ethyldiaminetetraacetic acid(EDTA), sodium citrate and heparin. The separate frequencies of PMN leucocytes from buffy coat layer was 60.4$\pm$9.6%(heparin), 56.8$\pm$11.8%(sodium citrate), 30.6$\pm$14.1%(ACD) and 6.2$\pm$3.7%(EDTA), in order. Those from packed RBC layer monitored with EDTA, ACD, sodium citrate and heparin was 85.0$\pm$4.7%, 84.3$\pm$5.5%, 83.8$\pm$6.5% and 76.3$\pm$7.7%, respectively. The Ficoll-hypaque(FH) density gradient method was used to remove a small part of lymphocytes and/or monocytes from leucocytes in packed RBC layer. With the result that it increased separate frequency of PMN leucocytes from EDTA(89.9$\pm$2.4%), ACD(89.5$\pm$3.6%), and sodium citrate(83.6$\pm$10.3%) than heparin(68.4$\pm$13.9%). These results indicate that the use of EDTA and ACD as anticoagulant Is suitable for the separation of PMN leucocytes from bovine peripheral blood, and that the FH density gradient method is able to increase the separate frequency of PMN leucocytes from packed RBC layer.

• Fisheries and Aquatic Sciences
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• 제5권4호
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• pp.258-262
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• 2002

Effects of Escherichia coli genomic DNA on the production of phagocyte activating supernatants by the head kidney leucocytes isolated from olive flounder (Paralichthys olivaceus) were investigated. Phagocyte activating activity of the supernatants was estimated by. measuring reactive oxygen species (ROS) production in target head kidney phagocytes. All supernatants from olive flounder head kidney leucocytes-stimulated with E. coli DNA induced significantly (P<0.01) higher ROS production from target pagocytes than the unstimulated control supernatant. Maximum enhancement of chemiluminescent response was observed $5.0-10.0{\mu}g\;mL^{-1}$ of bacterial DNA while the increment ability was decreased significantly (P<0.01) at the concentration of $20.0{\mu}mL^{-1}$. The results demonstrate that olive flounder head-kidney leucocytes stimulated with bacterial DNA release a soluble phagocyte activating cytokines capable of enhancing the respiratory burst activity from target phagocytes.

• 대한수의학회지
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• 제29권4호
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• pp.437-443
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• 1989

This experiment was carried out to investigate the hematological and blood chemical values in dairy cattle during the dry and lactating period. Blood was collected from six healthy dairy cattle in Gyeongnam province. Leucocytes count, hemoglobin concentrations and the percentage of packed cell volume were lowest at the early lactation period. Leucocytes count was not affected during dry the to lactating period. The differential count of eosinophilic leucocytes was low at the late lactation period, while the basophilic leucocytes was high at the dry period. Inorganic phosphorus value was below under normal level. Creatinine value was from 1 to 2mg/100ml of serum. Positive reaction to CRP was shown in normal dairy cattle. The values of AST and ALT were higher during the dry than lactating period. r-GTP and total cholesterol tend to be decreased during the lactating period. CPK value was not affected for dry and lactating period.

• 한국동물학회지
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• 제7권1호
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• pp.29-32
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• 1964

A study on chromosome of leucocytes in blood cultures derived from 6 normal Korean was performed . Exact chromosome counts were carried out on 205 cells in male, 211 in female , of which 86.05% revealed a chormosome mordal number of 46. On the basis of relative chromosome lengths and position of centromeres, the Karyotype that the human chromosomes were classified into 7 groups with 22 airs of autosome and one pair of sex chromosome was determined accoridng to the method of denver report. The chromosome number on metaphase was observed in short term cultures of leucocytes from the peripheral blood of 2 patients with chronic granulocytic leukemia and 1 patient with acute granulocitic leukemia . and the chromosome morpholoogy was also investigated in one acute leukemic patient. In all leukemic cases the leucocytes showed the constant value of 46 in the stem -line of chormosome number. But the frequency of cells with 46 chromosomes appeared in the 3 cases was 67.30% in average with a slightly higher range in hypo-andhyper-diploid chromosome numbers than in normal human, The idiogram analysis did not show any abnormality of chromosome in acute leukemic cells.

• 한국어병학회지
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• 제8권1호
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• pp.47-55
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• 1995

한국산 메기 (Silurus asotus) 의 말초혈액중의 백혈구를 May-Grunwald Gimesa 염색에 의한 형태학적 특성과 Peroxidase, Alkaline phosphatase, Acid phosphatase, PAS 반응에 의한 세포 화학적 성상 및 Escherichia coli 포르말린 사균에 대한 식작용의 유무에 따라 분류하여 각 혈구의 조성을 산출하였다. 말초혈액중의 백혈구는 호중구, 단구, 림프구 및 전구의 4 종으로 구분 되어졌다. 호중구는 직경 $12\sim15{\mu}m$의 원형의 세포로, 핵은 대부분이 원형, 난형 또는 2-3개의 분엽핵상으로 호중성의 세포질내에 편재되어 있었고, Peroxidase 와 PAS 반응에는 양성, Alkaline phosphatase 와 Acid phosphatase 반응은 음성이었으며 식작용을 표시하였다. 단구는 직경 $12\sim15{\mu}m$의 원형 또는 불규칙한 모양의 세포로 핵은 완두콩 또는 불규칙한 모양으로 모든 세포화학 반응에 음성이었지만, 식작용을 나타냈다. 림프구는 직경 $5.5\sim8.0{\mu}m$의 원형으로 핵이 세포의 대부분을 차지하고 있었으며, 모든 세포화학 반응과 식작용은 음성이었다. 전구는 $5.8\sim7.2{\times}7.2\sim14.4{\mu}m$의 난원형 또는 방추형의 세포로 핵은 세포의 형태에 동일하였으며, 모든 세포화학반응과 탐식능이 음성이었다. 말초혈액중의 각 혈구와 조성은 림프구가 제일 많고 다음이 전구, 호중구, 단구의 순으로 많았다.

• Archives of Pharmacal Research
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• 제6권2호
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• pp.123-131
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• 1983

Polysaccharide fractions were prepared from Ginseng root, Mori Radicis Cortex (M. R. C. ), Phellodendri Cortex (Ph. C. ), Sappan Wood (S. W. ) and Tigli Semen (T. S.). Water extract was also prepared from the mixture of ph. C., S. W. and T. S. Ginseng polysaccharide and water extract of the mixture showed marked antitumor activity against sarcoma 180. Ginseng polysaccharide showed a mild increasing effect on the number of circulating leucocytes and a marked increasing effect on the number leucocytes and a marked increasing effect on the number of plaque forming cells (PEC). Polysaccharides from ginsing root, S. W., Ph. C. + T. S. and water extract of the mixture showed dramatic inducing activities of periotoneal exudate cells (PEC), polymorphonuclear leucocytes (PMN) and macrophages. These results suggest the possibility that water extract of the mixture may have the lentinan like effect and ginseng polysaccharide may have stimulating effects on the general immune system.

• 한국어병학회지
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• 제13권2호
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• pp.103-110
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• 2000

Quaternary ammonium compounds(QACs)가 뱀장어의 면역반응에 미치는 영향을 측정하기 위해서 뱀장어의 혈액으로부터 림프구를 분리하여 증식능을 측정하였으며 또한 신장으로부터 탐식세포를 분리하여 탐식능, pinocytosis 및 superoxide anion활성능 등을 측정하였다. 림프구의 증식능은 mytogen(T-cell mitogen: ConA and PHA, B-cell mitogen: LPS) 및 QACs처리농도에 따라 별다른 차이를 보이지 않았다. 한편 신장 유래의 대식세포의 세균살해능은 QACs첨가농도에 따라 유의성 있게 증가되었다. 이러한 결과는 QACs이 작동대식세포로의 분화 및 활성을 촉진시켜서 탐식능, pinocytes 및 superoxide생성 등의 분비를 증가시킨 것으로 사료된다. 이상의 결과로 미루어 QACs가 어체의 면역반응에 관여하는 작용기전은 면역작동세포의 수를 증가시키기보다는 세포의 기능활성을 증강시킴을 알 수 있다.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 1997년도 수산관련공동학술대회발표요지집
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• pp.291-292
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• 1997

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 1997년도 수산관련공동학술대회발표요지집
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• pp.293-294
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• 1997