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The Bacillus subtilis and Lactic Acid Bacteria Probiotics Influences Intestinal Mucin Gene Expression, Histomorphology and Growth Performance in Broilers

  • Aliakbarpour, H.R.;Chamani, Mohammad;Rahimi, G.;Sadeghi, A.A.;Qujeq, D.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.9
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    • pp.1285-1293
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    • 2012
  • The aim of the present study was to evaluate the effect of commercial monostrain and multistrain probiotics in diets on growth performance, intestinal morphology and mucin gene (MUC2) expression in broiler chicks. Three hundred seventy-eight 1-d-old male Arian broiler chicks were allocated in 3 experimental groups for 6 wk. The birds were fed on a corn-soybean based diet and depending on the addition were labeled as follows: control-unsupplemented (C), birds supplemented with Bacillus subtilis (BS) and lactic acid bacteria (LAB) based probiotics. Each treatment had 6 replicates of 21 broilers each. Treatment effects on body weight, feed intake, feed conversion ratio and biomarkers such as intestinal goblet cell density, villus length, villus width, and mucin gene expression were determined. Total feed intake did not differ significantly between control birds and those fed a diet with probiotics (p>0.05). However, significant differences in growth performance were found. Final body weight at 42 d of age was higher in birds fed a diet with probiotics compared to those fed a diet without probiotic (p<0.05). Inclusion of Bacillus subtilis based probiotic in the diets also significantly affected feed conversion rate (FCR) compared with control birds (p<0.05). No differences in growth performance were observed in birds fed different types of probiotic supplemented diets. Inclusion of lactic acid bacteria based probiotic in the diets significantly increased goblet cell number and villus length (p<0.05). Furthermore, diets with Bacillus subtilis based probiotics significantly increased gene expression (p<0.05), with higher intestinal MUC2 mRNA in birds fed diet with probiotics compared to those fed the control diet. In BS and LAB probiotic fed chicks, higher growth performance may be related to higher expression of the MUC2 gene in goblet cells and/or morphological change of small intestinal tract. The higher synthesis of the mucin gene after probiotic administration may positively affect bacterial interactions in the intestinal digestive tract, intestinal mucosal cell proliferation and consequently efficient nutrient absorption.

Effect of Nutrient Solution Strength on Growth of Phalaenopsis in an Ebb and Flow System (Ebb and Flow 시스템을 이용한 호접란 수경재배시 생장에 미치는 배양액 농도의 영향)

  • An, Dong-Choon;Park, Seon-Hye;Been, Chul-Gu;Jeong, Byoung-Ryong
    • Journal of Bio-Environment Control
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    • v.16 no.3
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    • pp.199-204
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    • 2007
  • This experiment was conducted to investigate the effect of nutrient solution strength on the growth of miniature multiflora P. amabilis and P. Taisuco Red Jewel at two growth stages during four months cultivation in an ebb and flow system. Early stage plants gained the biggest leaf length and width when nutrient solution strength was EC 0.5 or $1.0dS{\cdot}m^{-1}$ in P. amabilis, and EC $1.5dS{\cdot}m^{-1}$ in P. Taisuco Red Jewel. Root length and weight were the greatest when nutrient solution strength was EC $0.5dS{\cdot}m^{-1}$ in both cultivars and this trend was also found in middle stage plants. Fresh and dry weights of leaves increased as nutrient solution strength was elevated and were the greatest when nutrient solution strength was EC $1.5dS{\cdot}m^{-1}$ in P. amabilis. On the contrary elevation of nutrient solution strength decreased fresh weight of roots, being the greatest when nutrient solution strength was EC $0.5dS{\cdot}m^{-1}$. This trend was also found in P. Taisuco Red Jewel and P. amabilis at middle growth stage. The T/R ratio and chlorophyll content increased with nutrient solution strength and were the greatest regardless of cultivar and growth stage when nutrient strength was EC $1.5dS{\cdot}m^{-1}$.

Structural Engineering Study of 60M Twin-hull typed Car-ferry (60M급 쌍동형 카페리 구조 엔지니어링 고찰)

  • Lee, Jung-Ho;Kim, In-Chul;Seo, Kwang-Chul
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.23 no.5
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    • pp.532-540
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    • 2017
  • This paper suggests study of basic structure design and structural analysis for the twin car-ferries. The rules and methodology for the strength analysis of medium and small sized high speed vessels with a length of more than 50m and a length/width ratio of more than 12, such as car-ferries, have not been clarified yet. Therefore, in this paper, the members scantling were carried out using the KR Rule, and the car-ferry exclusive vessel standard were further applied to verify the structural strength the design. The scantling of the members is based on the Korea Classification standards, and the car-ferries standards were additionally applied to verify the structural strength of the design. Especially, the ultimate strength of hull girder was additionally carried out by using car-ferry exclusive vessel standard, and proposed to overcome ambiguity of design by existing standard. The results of this study are expected to be useful as basic data related to structural design and structural analysis of high speed twin-hull car-ferries. The present car-ferry design has a sufficient safety margin in strength point of view according to the KR rule.

Characters of Fracture-filling Minerals in the KURT and Their Significance (한국원자력 연구원 지하처분연구시설(KURT)의 단열충전광물 특성과 그 의미)

  • Lee, Seung-Yeop;Baik, Min-Hoon
    • Journal of the Mineralogical Society of Korea
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    • v.20 no.3
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    • pp.165-173
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    • 2007
  • The KAERI Underground Research Tunnel (KURT) located in KAERI (Korea Atomic Energy Research Institute) was recently constructed following the site investigation in 2003. Its dimension is 180 m in length, 6 m in width, and 6 m in height, and it has a horseshoe-like cross-sec-lion and is located in the ground to the depth of 90 m. When the tunnel was dug into the ground with 100 m in length, fresh rocks, weathered rocks and fracture-filling materials were taken and examined by mineralogical and chemical analyses. There are phyllosilicate minerals such as illite, smectite and chlorite including calcite, which are filling some faults and cracks of the KURT rock. The illite and smectite usually coexist in the fracture, where their content ratio is different according to which mineral is predominant. There are high concentrations of U and Th in the rocks coated with iron-oxides and filled with secondary materials as compared with those in the fresh rocks. It seems that the radionuclides, which are slowly leached from the parent rocks or exist as a dissolved form in the groundwater and hydrothermal solution, may have been migrated along the fractures and thereafter selectively sorbed and coprecipitated on the iron-oxides and the fracture-filling materials. These results will be very useful far the evaluation of environmental factors affecting the nuclides migration and retardation when long-term safety is considered to the geological disposal of high-level radioactive wastes in the future.

Effect of Red LEDs during Healing and Acclimatization Process on the Survival Rate and Quality of Grafted Tomato Seedlings (토마토 접목묘의 순화과정에서 적색LED광조사가 접목 활착율과 묘소질에 미치는 영향)

  • Vu, Ngoc-Thang;Kim, Young-Shik;Kang, Ho-Min;Kim, Il-Seop
    • Journal of Bio-Environment Control
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    • v.23 no.1
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    • pp.43-49
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    • 2014
  • This study was designed to determine the effect of red LEDs during healing and acclimatization process on the survival rate and quality of grafted tomato seedlings. Red LEDs and no light (darkness) were used for treating three rootstock cultivars, which are 'B-Blocking', 'Kanbarune', 'High-power' in healing room. Results showed that survival rates of grafted seedlings in red LEDs were higher than those in no light treatment. Significant variation on survival rates of rootstock cultivars was observed in no light treatment but there was not significant variation in red LEDs treatment. Light treatment also reduced the percentage of infected plants, except for the 'Kanbarune' cultivar. Seedling quality in red LEDs was better than that in no light treatment by improving growth parameters such as plant height, leaf length, leaf width, leaf area, fresh and dry weight of shoot and root. Light treatments and rootstock cultivars did not affect number of leaves, leaf chlorophyll value and T/R ratio of seedlings, but seedlings in red LEDs were significantly more compact than those in no light treatment. Moreover, the root morphology of seedlings such as total root surface area, total root length, and number of toot tips in red LEDs was also greater than that in no light treatment.

Development of Solenoid RF Coil for Animal Imaging in 3T High Magnetic Field MRI (고자장 3T MRI 장비에서 동물영상을 위한 솔레노이드 RF코일 개발)

  • Lee, Hong-Seok;Woo, Dong-Cheol;Min, Kwang-Hong;Kim, Yong-Kwon;Lee, Heung-Kyu;Choe, Bo-Young
    • Investigative Magnetic Resonance Imaging
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    • v.11 no.1
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    • pp.20-26
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    • 2007
  • Purpose : The purpose of the present study was to develop and optimize solenoid coil for animal- model in 3 T MRI system and investigate and compare with the birdcage coil concerning the image quality with the various parameters such as SNR and Q-factor. Materials and Methods : Solenoid coil for animal-model was made on the acryl structure (diameter 4 cm, length 10 cm) 3 times-winding cooper tape of width 2 cm, thickness 0.05 cm and length 10 cm with 2 cm interval between winded tapes. Capacitors from 2 pF to 100 pF were used, and the solenoid coil was designed for receiver only coil. Results : SNR of the developed solenoid was 985 in CuSO4 0.7 g/L and 995 in rat experiment. Q-factor was 84-89 in unloaded condition and 203-206 in loaded condition. Conclusion : The resolution of the image obtained from solenoid was relatively higher than that of the conventional birdcage coil. In addition, the homogeneity of RF field by coil simulation was significantly excellent. The present study demonstrated that the solenoid coil could be useful to obtain small animal images with better contrast, resolution, visibility than images from birdcage.

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The Preliminary Study of the Secondary Precipitates from Samsanjeil and Sambong Mine, Goseong, Gyeongnam (경남 고성군 삼산면 삼산제일광산과 삼봉광산 주변 하천 침전물에 관한 예비 연구)

  • Cho, Hyen-Goo;Chang, Byoung-Jun;Kim, Soon-Oh;Choo, Chang-Oh
    • Journal of the Mineralogical Society of Korea
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    • v.19 no.3 s.49
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    • pp.129-138
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    • 2006
  • In this study, we identified the secondary precipitates from Samsan-jeil and Sambong mine, Goseong, Gyeongnam by means of scanning electron microscopy, electron probe microanalysis and X-ray powder diffraction analysis. Copper sulfide minerals had been produced from the mines during last few decades, however they are not worked. White and blue precipitates were found at the downstream of mine rock dump at Sambong mine and green one was at Samsan-jeil mine. The white precipitate covered the host rock surface with thickness of $30{\mu}m$, and is a kind of diatom with $10{\mu}m$ in length and $3{\mu}m$ in width. It is a species Fragilaria constuens, which is contained a order Pennales(pennate diatom) and lives in fresh water. The blue precipitate is the alteration product of chalcopyrite. It resultes in the increase in the ratio Cu:Fe from 5 to 13. The green precipitate has worm-like morphology with $10{\sim}20nm$ in diameter and $200{\sim}300nm$ in length. It is mainly composed of secondary copper sulfate such as woodwardite. However, it could be formed by the activity of microorganism, because the copper content is more than any secondary copper sulfate reported in copper sulfide mine. In order to identity the green precipitate exactly, the further research is needed.

Effect of Cutting Times according to Growth Stage in Sorghum X Sudangrass Hybrid on Frequence of Use, Growth Characteristics, Forage Production and Crude Protein Yield (생육단계별 예취 시기가 수수 X 수단그라스 교잡종의 이용회수, 생육특성, 수량 및 조단백질수량에 미치는 영향)

  • Jeon Byong Tae;Lee Sang Moo
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.25 no.1
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    • pp.33-42
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    • 2005
  • A field experiment was conducted to evaluate growth characteristics, forage production and crude protein yield according to cutting time of Soghum ${\times}$ Sudangrass Hybrid, and decide ideal harvesting time for use of soiling and silage. Experiment design was arranged with 7 different treatment T1(150 m), T2(200 cm), T3(boot), T4(heading), T5(milk), T6(dough) and T7(yellow stage), as a randomized block design. The results were as fellows : Cutting times of utilization during the course of a year was 4 times at T1 and T2, 3 times at T3 and T4, and 2 times at T5, T6 and T7. Accumulative plant length was the highest at T2(666cm), but T3 was the lowest as 402 cm. Mean Leaf length was the highest at T5(82.1 m) and lowest at T7(T1.8 m). Mean leaf width was the highest at T2 and lowest at T6. Stem diameter was orderly ranked as T3(10.7 mm)>T1(9.5)>T2, T5(9.3>T6(8.9)>T7(8.6)>T4(8.5). Stem hardness was orderly ranked as $T7(3.2 kg/cm^2$>T5, T6(2.3)>T3, T4(1.5)> T2(0.6)>T7(8.6)>T1(0.5). Mean of leaf number and leaf ratio was the highest at $T3(8.1\%)$ and $T2(45.3\%)$, respectively. The highest yield of fresh and dry matter was obtained at T4 and T6 as 113,246 and 24,249 kg/ha, respectively(P<0.05), and e lowest at T7 and T1 as 82,675 and 13,006 kg/ha, respectively(P<0.05). Crude protein yield was highest at T6(1.456 kg/ha) and lowest at T3 as 1,189 kg/ha. As mentioned above the result T1, T2 and T3 could be recommended as use of soiling, and T5, T6 and T7 as silage.

Yield and Size of Enlarged Rhizome among Different Kinds of White Lotus (백련 종류에 따른 연근 크기와 수확량)

  • Im, Myung-Hee;Park, Yong-Seo;Cho, Ja-Yong;Park, Sam-Gyun;Yun, Jae-Gill;Jang, Hong-Gi;Heo, Buk-Gu
    • Korean Journal of Plant Resources
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    • v.22 no.1
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    • pp.42-48
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    • 2009
  • This study was conducted to examine the difference in yield and size of enlarged rhizome according to the kinds of native white lotuses. Four white lotuses (Nelumbo nucifera) were collected from 4 different sites, Jeonbuk Jeonju ('Garam'), Jeonnam Muan ('Hoesan'), Chungnam Buyeo ('Ihnchuisa'), and Chungnam Cheongyang('Cheongyang'). Each enlarged rhizome was planted in an area of 3.3 $m^2$ on 10. April, 2007 and digged out on 19. February, 2008. The yield of enlarged rhizome was in the order of Hoesan (3.0 kg/$m^2$), Cheongyang (2.5 kg/$m^2$), Garam (2.0 kg/$m^2$) and Ihnchuisa (1.0 kg/$m^2$). The number of enlarged rhizomes was in the order of Ihnchuisa (4.9/$m^2$), Garam (4.3/$m^2$), Hoesan (4.2/$m^2$) and Cheongyang (4.1/$m^2$). Total number of nodes in the enlarged rhizomes were much more in the order of Hoesan (17.0/$m^2$), Ihnchuisa (16.0/$m^2$), Cheongyang (15.3/$m^2$) and Garam (14.1/$m^2$). Component ratio of enlarged rhizome length below 50cm for Ihnchuisa, Garam, Hoesan and Cheongyang were 76.3%, 53.0%, 38.2% and 40.3% respectively. Native white lotus had a longer enlarged rhizome in the first node than the other nodes, and their enlarged rhizome length and width became thin. And it became thick and short from the second node of enlarged rhizome. Accordingly the amount of enlarged rhizomes 'Hoesan' was extremely much than others.

Effect of Acoustic Emission During a Fatigue Test with Defect for Type II Gas Cylinder (피로시험시 발생하는 음향방출신호를 이용한 Type II Gas Cylinder의 손상평가)

  • Jee, Hyun-Sup;Lee, Jong-O;Ju, No-Hoe;So, Cheal-Ho;Lee, Jong-Kyu
    • Journal of the Korean Institute of Gas
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    • v.16 no.2
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    • pp.18-24
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    • 2012
  • This research seeks to evaluate damage on type II gas cylinder by an acoustic emission test when executing 20000 cycles fatigue test and thereafter burst test. Used gas cylinders in the experimental are three types as follows; one is sound cylinder, others are cylinders which contain longitudinal and transverse artificial defect. The size of artificial defect is a depth of 3 mm, width of 3 mm and length of 50 mm. In the case of the cylinder which artificial defect, unlike the expectation that it will burst in low pressure, the burst pressure of the cylinder did not differ much according to whether or not there were defects. However, when there was longitudinal defect, the location of burst was near the location of defect. This leads to the effect in which the thickness of the composite material becomes thinner according to the length of the longitudinal defect and this is judged to have an effect on the location of initiation and growth of crack in the liner. Also, for the acoustic emission signal, when there is longitudinal defect, the ratio of an event occurring at defect position among overall hits is more than 50 %, and the source location also accords very precisely with defect position.