• Journal of Forest and Environmental Science
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• v.31 no.2
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• pp.91-108
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• 2015

The study investigates herbage communities along the altitudinal gradient in northwest Himalaya and highlights the effect of trees on its understory floristic diversity and phytosociology. The study was conducted in nine forests at three elevations viz., chir pine (Pinus roxburghii), mixed and khair (Acacia catechu) forests at elevation $E_1$ (850-1150 m), chir pine, mixed and ban oak (Quercus leucotrichophora) forests at elevation $E_2$ (1151-1600 m) and ban oak, mixed and chir pine forests at elevation $E_3$ (>1600 m) in a sub-watershed located in Solan district of Himachal Pradesh, India. These were compared grasslands located adjacent to forests. In all, 20 grass, 3 sedge, 2 forb and 4 legume species were recorded in study sites. Jaccard's coefficient of herbage vegetation was highest between chir pine forests and grasslands, and lowest in khair and ban oak forests. TWINSPAN dendrogram of herbage composition exhibited three subtypes with Apluda mutica, Arundinella nepalensis and Dichanthium annulatum as indicator species. Three groups of plant communities were identified on the basis of their moisture requirement. Peak density and basal area of herbage in forests and grasslands occurred by September. Density and basal area of herbage in grasslands at different elevations ranged from 649.6 to $1347.9tillers/m^2$ and 30.0 to $65.7cm^2/m^2$, respectively, while, in forests it varied from 351.2 to $1005.3tillers/m^2$ and 14.9 to $43.9cm^2/m^2$, respectively. Density and basal area of the herbage in plant communities decreased along the elevation. Under trees in forests the density of herbage decreased up to 77% and basal area up to 62% of their respective values in grasslands.

• The Korean Journal of Ecology
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• v.19 no.2
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• pp.107-123
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• 1996

The position effects on the abortion of flowers, fruits, and ovules were examined in relation to the resource limitation hypothesis using a temperate legume species, Vicia cracca. Fifty plants were randomly sampled from a natural population located at the Belmont Conservation Site in Belmont, MA, USA. Due to indeterminant growth and acropetal flowering of this species, the position of flower or fruit on the plant was able to be coded by the combination of architectural levels such as plant half (lower and upper half) and inflorescence half (lower and upper half) within each plant half. Overall, only 17.3% of the flowers on an inflorescence began to develop into fruits, while overall 36.0% of the fruits initiated failed to mature. Consequently, 11.5% of the flowers successfully matured into fruits. The mean flower number per inflorescence differed significantly among plants as well as between plant halves. Although the probability of fruit initiation was higher in the lower halves of both plant and inflorescence than in the upper halves of the latter, fruit abortion rate differed significantly only between plant halves. The overall mean seed set was 33.6%, implying that about two out of six ovules in a fruit matured into seeds. The seed set was independent of both plant identity and architectural level unlike other reproductive characters examined. When the ovule developmental stage was examined on data pooled over all fruits, the proportion of ovules in varying developmental stages decreased in order of early abortion, seed maturation, late abortion, and seed predation (48.4, 34.9, 12.2, and 4.5%, respectively). A within-fruit ovule position was also used as a class variable for the analysis of position effects on ovule development. All architectural levels considered exerted singificant influence over the ovule development. In particular, ovule development was strongly affected by the within-fruit ovule position. Ovules in both extreme ends within a fruit tended to abort early, while those in the middle position were more likely to mature into seeds. The strong position effects detected from the flowering to seed maturation stage were interpreted as an indication of competitive interaction among reproductive characters which are largely constrained by plant architecture.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.2
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• pp.127-132
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• 1999

No comprehensive study of yield and quality losses of forage legumes harvested and cured in spring has been conducted in Korea, therefore, this experiment was carried out to gain information on yield and quality, losses of alfalfa(Medicgo sativa L.), crimson clover (Trifolium incarnatum L.), and red clover(Trifolium pratense L.) during field curing in spring(mid-May). Alfalfa was highest in dry matter content of 15% at harvest, while crimson clover and red clover had 12.4 and 11.5%, respectively. Species differences for the dry matter content were maintained and consistent trends were observed during the field curing. Alfalfa took seven days to reach dry matter content over 80%, while both crimson clover and red clover took eight days. Crimson clover and alfalfa tended to show a higher leaf-stem ratio than red clover based on dry matter, but red clover showed a higher leaf-stem ratio than alfalfa and crimson clover based on crude protein content. Losses in dry matter of alfalfa from leaf shattering were higher than crimson clover and red clover, and similar trends were detected losses in crude protein for three different legumes. Fresh legume forages averaged higher in clude protein(CP), total digestible nutrients(TDN), and relative feed value(RFV), while dry legume forages averaged lower in acid detergent fiber(ADF) and neutral detergent fiber(NDF). Results of the experiment indicate that hay curing in spring affects crude protein concentration more than ADF and NDF, and this is due, in part, to leaf shattering caused by field operations.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.1
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• pp.61-70
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• 2008

The object of this experiment was to investigate the food habits of sika deer fed with various roughage sources. The feeding trials were conducted at Chungnam National University and Unbong Animal Genetic Resources Station in 2007. The experimental roughages sources were five including 25 species in all; grass sources; 5 species(Lolium perenne, Festuca arundinacea, Poa pratensis, Agrostis alba and mixed grass), legume sources; 5 species(Trifolium pratense, Medicago sativa, Trifolium repens, Melilotus officinalis and Vicia villosa), browse sources; 5 species(Quercus serrata Thunb., Prunus jamasakura Sieb., Quercus aliena Blume, Robinia pseudoaccacia and Pinus densiflora), weed sources; 5 species(Artemisia princeps Pampanini, Erigeron canadensis, Alopecurus aegualis Sobolewski, Echinochloa crusgalli var. frumentacea(Roxb.) Wight and Rumex crispus), and native grass sources; 5 species(Zoysia japonica Steudel, Agropyron tsukushiense var. transiens(Hack) Ohwi., Arundinella hirta(Thunb.) Tanaka, Miscanthus sinensis Anderss and Phragmites comunis Trin). Five sika deer were selected which had nearly the same body weight(average 92.5kg) as experimental animal. Experimental roughages were prepared by 0.5kg(DM basis) per 1 species fed to sika deer, and the experiment was carried out from 08:00 to the next day 06:00. The chemical composition and dry matter digestibility of each roughage source and species were significantly different at the sampling area, plant species, growth stages and cutting period. The sika deer ate more roughages which had low fibrous contents, but high dry matter digestibility. Among all the 25 species of roughages, the favorite intake species ranking by sika deer was observed like this: Quercus aliena Blume, Prunus jamasakura Sieb, Quercus serrata Thunb. and Trifolium pratense, respectively, and the lower intake species was Phragmites comunis Trin. On the other hand, compared to each roughage source, total intake amount by sika deer was showed as browse(36.5%), Legumes(34.2%), grasses(14.0%), native grasses(13.5%) and weeds(1.8%), respectively. Based on the result, the food habits of sika deer seems to be closer to typically browser type.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.4
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• pp.313-322
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• 2007

The object of this experiment was to investigate the food habits of dairy goats fed with various roughage sources. The experimental trials were conducted at Chungnam National University in 2007. The experimental roughages include five sources and 25 species in all; grass sources: 5 species (Lolium perenne, Festuca arundinacea, Poa pratensis, Agrostis alba and mixed grass, legume sources: 5 species (Trifolium pratense, Medicago sativa, Trifolium repens, Melilotus officinalis and Vicia villosa), browse sources : 5 species (Quercus serrata Thunb., Prunus jamasakura Sieb., Quercus aliena Blume, Robinia pseudoaccacia and Pinus densiflora), weed sources: 5 species (Artemisia princeps Pampanini, Erigeron canadensis, Alopecurus aegualis Sobolewski, Echinochloa crusgalli var. frumentacea (Roxb.) Wight and Rumex crispus), and native grass sources: 5 species (Zoysia japonica Steudel, Agropyron tsukushiense var. transiens (Hack) Ohwi., Arundinella hirta (Thunb.) Tanaka, Miscanthus sinensis Anderss and Phragmites comunis Trin). Ten dairy goats(Saanen) were selected which had nearly the same body weight(average 31kg). Experimental roughages were prepared by 0.5kg per 1 species fed to dairy goats, and the experiment was carried out from 09:00 to 18:00. The chemical composition and dry matter digestibility of each roughage source and species were significantly different at the sampling area, plant species, growth stages and cutting period. The dairy goats ate more roughages which had low fibrous contents, but high dry matter digestibility. Among all the 25 species of roughages, the favorite intake species order by dairy goat was observed like this: perennial ryegrass, tall fescue and red clover, respectively, and the lower intake species order was Rumex crispus. On the other hand, compared to each roughage source, total intake amount by dairy goats was showed as forage grasses (59.0%) which contained grasses(33.3%) and legumes (25.7%), browse (19.6%), weeds (15.9%) and native grasses (5.5%). Based on the result, the food intake type of dairy goats seems to be closer to grazer type.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.2
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• pp.119-128
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• 2008

The object of this experiment was to investigate the food habits of Korean native goats fed with various roughage sources. The feeding trials were conducted at Chungnam National University and Unbong Animal Genetic Resources Station in 2007. The experimental roughages sources were five including 25 species in all; grass sources: 5 species (Lolium perenne, Festuca arundinacea, Poa pratensis, Agrostis alba and mixed grass, legume sources: 5 species (Trifolium pratense, Medicago sativa, Trifolium repens, Melilotus officinalis and Vicia villosa), browse sources: 5 species(Quercus serrata Thunb., Prunus jamasakura Sieb., Quercus aliena Blume, Robinia pseudoaccacia and Pinus densiflora), weed sources: 5 species(Artemisia princeps Pampanini, Erigeron canadensis, Alopecurus aegualis Sobolewski, Echinochloa crusgalli var. frumentacea (Roxb.) Wight and Rumex crispus), and native grass sources: 5 species(Zoysia japonica Steudel, Agropyron tsukushiense var. transiens(Hack) Ohwi., Arundinella hirta(Thunb.) Tanaka, Miscanthus sinensis Anderss and Phragmites comunis Trin). Ten Korean native goats were selected which had nearly the same body weight(average 30.6kg, 남) as experimental animals. Experimental roughages were prepared by 0.5kg per 1 species fed to Korean native goats, and the experiment was carried out from 08:00 to 20:00. The chemical composition and dry matter digestibility of each roughage source and species were significantly different at the sampling area, plant species, growth stages and cutting period (p<0.05). The Korean native goats ate more roughages which had low fibrous contents, but high dry matter digestibility. Among all the 25 species of roughages, the favorite intake species order by Korean native goats was observed like this: Quercus serrata Thunb., Quercus aliena Blume, Trifolium repens, and Pinus densiflora and Artemisia princeps Pampanini which was lower intake compared to other domestic herbivores. On the other hand, compared to each roughage source, total intake amount by Korean native goats was showed as browse(29.9%), legumes(23.0%), weeds(21.6%), grasses(21.5%) and native grasses(4.0%), respectively. Based on the result, the food habit of Korean native goats seems to be closer to browser type.

• Korean Journal of Plant Resources
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• v.36 no.4
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• pp.369-380
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• 2023

Developing and breeding improved legume-based food resources require collecting useful genetic traits with heritability even though requiring some time-consuming, costly, and labor intensive. We attempted to infer heritability of nine genetic traits-days to flowering, days to maturity, period from flowering to maturity, the number of seeds per pod, 100-seeds weight, and four contents such as crude protein, crude oil, crude fiber, and dietary fiber-using 455 homologous chloroplast gene sets of six species of legumes. Correlation analysis between genetic trait differences and phylogenetic distance of homologous gene sets revealed that days to flowering, the number of seeds per pod, and crude oil content were influenced by genetic factors rather than environmental factors by 62.86%, 69.45%, 57.14% of correlated genes (P-value ≤ 0.05) and days to maturity showed intermediate genetic effects by 62.42% (P-value ≤ 0.1). The period from flowering to maturity and 100-seeds weight showed different results compared to those of some previous studies, which may be attributed to highly complicated internal (epistatic or additive gene effects) and external effects (cultural environment and human behaviors). Despite being slightly unexpected, our results and method can widely contribute to analyze heritability by including genetic information on mitochondria, nuclear genome, and single nucleotide polymorphisms.

• The Korean Journal of Food And Nutrition
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• v.28 no.4
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• pp.728-736
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• 2015

Cronobacter species (Cronobacter spp.), previously known as Enterobacter sakazakii, are gram negative food borne pathogenic bacteria. They pose a very high risk of infection to neonates and immuno-compromised individuals and can affect the human central nervous system. Consequently, survivors often suffer from severe neurological impairment including hydrocephalus, quadriplegia, and developmental delays. Cronobacter spp. were not only isolated from plant food and products such as cereals, fruits, vegetables, legume products, herbs, and spices but also from animal source foods such as milk, meat, fish, and products made from these foods. Therefore, rapid detection of Cronobacter spp. is essential for food safety. Many detection methods have been developed since the Cronobacter spp. were first reported. However, the development of more rapid, sensitive, and easy-to-use detection methods for the Cronobacter spp. is required. In this review, our aim was to study and compare the available detection methods for Cronobacter spp., including culture-based, molecular biology-based, and immunology-based methods. This study will contribute to the development of new and rapid detection method for Cronobacter spp.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.1
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• pp.75-81
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• 1996

The dry matter (DM) disappearance and ruminal solubility of calcium (Ca), phosphorus (P), magnesium (Mg) and potassium (K) in eight Philippine forages were studied. The forages were: paragrass (Brachiaria mutica (Forsk.) Stapf), stargrass (Cynodon plectostachyum Pilger), napiergrass (Pennisetum purpureum Schumach), clopo (Calopogonium mucconoides Desv.), centrocema (Centrocema pubescens Benth.), gliricidia (Gliricidia sepium (Jacq.) Walp.), leucaena (Leucaena leucocephala (Lam.) de Wit.) and sesbania (Sesbania grandiflora (L.) Poir. Nylon bags with samples were incubated for 0, 3, 6, 12, 24, 48 and 72 h in rumen cannulated sheep. The 0-h bags were washed with deionized water. For the 0-h samples, 20.4, 17.2, 50.7, 52.2 and 80.1% of the DM, Ca, P, Mg and K was solubilized, respectively. At 3-h incubation period, DM disappearance was 10 percentage units higher than that of 0-h incubation whereas mineral disappearance increased by 43, 21, 30 and 13% for Ca, P, Mg and K, respectively. At 72-h incubation period, greater proportion of DM, Ca, especially in P, Mg and K was solubilized with a value of 73.8, 71.5, 85.6, 91.4 and 98.2%, respectively. The average particulate passage rate obtained in the present study was 1.9%/h where as the range of disappearance rates of various mineral elements were : 0.4 to 1.2%/h for Ca, 0.1 to 1.6%/h for P, 0.7 to 2%/h for Mg and 0.1 to 2%/h for K. The effective ruminal solubilization (ERS) of the macrominerals was calculated where particulate passage rate and disappearance rate of the various elements were included in the equation. The ERS of Ca, P, Mg and K was 50.0, 72.6, 83.9 and 94.5%, respectively. Species differences (p<0.05) on the various mineral solubilities were also observed. This study shows that ruminal solubility of macrominerals in selected Philippine forages is K > Mg > P > Ca.

• Journal of Microbiology and Biotechnology
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• v.20 no.2
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• pp.238-244
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• 2010

Rhizobia are well-known for their ability to infect and nodulate legume roots, forming a nitrogen-fixing symbiosis of agricultural importance. In addition, recent studies have shown that rhizobia can colonize roots and aerial plant tissues of rice as a model plant of the Graminaceae family. Here we show that rhizobia can invade tobacco, a model plant belonging to the Solanaceae family. Inoculation of seedling. roots with five GFP-tagged rhizobial species followed by microscopy and viable plating analyses indicated their colonization of the surface and interior of the whole vegetative plant. Blockage of ascending epiphytic migration by coating the hypocotyls with Vaseline showed that the endophytic rhizobia can exit the leaf interior through stomata and colonize the external phyllosphere habitat. These studies indicate rhizobia can colonize both below- and above-ground tissues of tobacco using a dynamic invasion process that involves both epiphytic and endophytic lifestyles.