• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.52-52
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• 2018

Development of herbicide resistant transgenic legume plants through Agrobacterium-mediated transformation has been worked in many previous studied. Plant regeneration after infection is the important step to obtain successful transgenic plants. Many attempts try to find the optimum media condition for plant regeneration after infection. However, the transformation efficiency of legume plants is still low. In this study, regeneration of some Korean legume species including two soybean cultivars (Dawon and Pungsan) and pea have been done with organogenesis which is used various kind of explants such as cotyledonary-nodes in soybean and bud-containing tissue in pea. We developed the optimum media condition for plant regeneration regulators under Agrobacterium-mediated transformation using different kind and various concentration of plant growth. As the results, B5 medium containing 2 mg/L of 6-benzylaminopurine was selected in this study for the optimum plant regeneration media. The segments were inoculated with Agrobacterium suspension harbored an IG2 vector containing bar gene which confers resistance to phosphinotricin (PPT) in 3, 5 and 7 days. The transformation efficiency was achieved in Dawon 3.03 % and pea 1.46 % with co-cultivation period of 7 days which is showed a high number of GUS positive expression period.

• Proceedings of the Zoological Society Korea Conference
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• 1996.10a
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• pp.106.1-106
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• 1996

No Abstract, See Full Text

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.10
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• pp.1429-1437
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• 2003

Browse tree legume leaves from Acacia spp (A. nilotica, A. tortilis, A. polyacantha), Dichrostachys sp, Flagea villosa, Piliostigma thonningii, Harrisonia sp were evaluated for nutritive potential (chemical compositions and degradability characteristics) compared to Gliricidia sepium. Effect of tannins anti-nutritive activity on digestibility was also assessed by polyethylene glycol (PEG) tannin bioassay. Crude protein (CP), ash, neutral detergent fiber (NDF), acid detergent fiber (ADF) and acid detergent lignin (ADL) differed (p<0.05) between legume foliages. Mean CP, ash, NDF, ADF and ADL for fodder species tested were 158, 92, 385, 145, and 100 g/kg DM, respectively. CP ranged from 115 (P. thonningii) to 205 g/kg DM (G. sepium). Acacia spp had moderate CP values (g/kg DM) of 144 (A. nilotica), to high CP in A. tortilis (188) and A. polyacantha (194) comparable to G. sepium. The forages had relatively lower fiber compositions. A. nilotica had (p<0.05) lowest NDF, ADF and ADL (182, 68 and 44) compared to P. thonningii (619, 196 and 130) g/kg DM, respectively. Except G. sepium, all fodder species had detectable high phenolic and tannin contents greater than 5% DM, an upper beneficial level in animal feeding and nutrition. Mean total phenolics (TP), total tannins (TT) and condensed tannins (CT) (or proanthocyanidins) for fodder species tested were 139, 113 and 43 mg/g DM, respectively. F. villosa had (p<0.05) lowest TP and TT of 65 and 56 mg/g DM, respectively, compared to A. nilotica (237 and 236 mg/g DM, respectively). The CT varied (p<0.05) from 6 (F. villosa) to 74 mg/g DM (Dichrostachys sp). In vitro organic matter (OM) degradability (OMD) differed (p<0.05) between fodder species. G. sepium had (p<0.05) high degradability potential compared to A. polyacantha that had (p<0.05) the lowest OMD values. Forage degradability ranked: G. sepium>A. nilotica>P. thonningi>F. villosa>Dichrostachys sp>A. tortilis>A. polyacantha. Addition of PEG resulted to (p<0.05) improvement in in vitro OM digestibility (IVD). Increase in IVD was mainly due to binding action of PEG on tannins; and represents potential nutritive values previously depressed by tannins anti-nutritive activity. Browse fodder has potential as sources of ruminal nitrogen especially for ruminants consuming low quality roughages due to high protein, lower fiber compositions and high potential digestibility. However, utilization of browse supplements in ruminants is hampered by high phenolic and tannin contents. Deactivation of tannin anti-nutritive activity, possibly by feeding tanniniferous browse with other readily available nitrogen sources to dilute tannin anti-nutritive activity could improve utilization of browse fodder supplements. Further studies are needed to assess browse fodder palatability and intake, and their effect on growth performance in ruminants.

• The Korean Journal of Mycology
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• v.20 no.3
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• pp.204-215
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• 1992

From 12 August to 21 November in 1991, 65 soil specimens were collected from nineteen leguminous plant roots throughout nine locations of four provinces. They were sieved to collect and identify the arbuscular mycorrhizal spores (four genera, 21 species). The species of Glomus were most commonly (47.6% of all observations) found, but the species of Gigaspora (about 4.8% of all observations) occurred infrequently or rarely rather than the species of the other genera. The most common arbuscular mycorrhizal species at nine locations were Acaulospora laevis and Glomus laminated spores cf. macrocarpus var. macrocarpus. All of nineteen legume plant species collected were found to be associated with the arbuscular mycorrhizal fungi . Cassia mimosoides var. nomame and Kummerowia striata in legume plants had the great frequency of arbuscular mycorrhizal fungi associated with their roots. The number of isolated spores were ranged from 0.3 to 10.0 spores per 10g of soil. The species diversity of arbuscular mycorrhizal spores at the disturbed areas were calculated to be bigger than those in the natural vegetation areas.

• Journal of the Korean earth science society
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• v.31 no.7
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• pp.691-697
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• 2010

Three legume fossils collected from the Miocene Duho Formation of Yeonil Group in the northern Pohang Beach, Gyeongsangbug-do, Korea were described as Albizia miokalkora Hu and Chaney. This species has only appearing in the Miocene floras of Korea, China and Japan until present. It has wide distribution from warm temperate to subtropical-tropical regions but, the diversity of the species of Albizia is not so high. It is considered that the fossil Albizia miokalkora Hu and Chaney might have adapted to the warm climate such as warm temperate climate and subtropical to tropical climate.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.5
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• pp.651-654
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• 2006

An experiment was conducted to investigate amino acid composition of three tropical legumes (Cooper (Glycine wightii), Tinaroo (Neonotonia wightii) and Siratro (Macroptilium atropurpureum)) at two different stages (pre-blooming and blooming stages). Chemical composition and totally 16 amino acids of these plants were analysed for comparison of their composition among species at different growing stages and characterizing the amino acid pattern of these legumes. Crude protein content of the plants ranged from 16% to 27% on a dry matter basis. The total amount of 16 amino acids analyzed in this experiment was highest at 89.7 mg/16 g N in Cooper at pre-blooming and lowest at 80.9 mg/16 g N in Glycine at blooming stage. Total amount of amino acids in each legume species tended to slightly decrease with their maturity but no statistical difference was found. The percentage of aspartic acid, glutamic acid and proline in the total amount of amino acids was dominant at 9% to 13%, and that of methionine was less than 1.6%. In this experiment it was concluded that three tropical legumes were rich in crude protein content and characterized by 16 different amino acids with lower sulfur-containing amino acid as methionine.

• The Plant Pathology Journal
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• v.30 no.2
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• pp.159-167
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• 2014

Molecular and biological characteristics of an isolate of Cucumber mosaic virus (CMV) from Glycine soja (wild soybean), named as CMV-209, was examined in this study. Comparison of nucleotide sequences and phylogenetic analyses of CMV-209 with the other CMV strains revealed that CMV-209 belonged to CMV subgroup I. However, CMV-209 showed some genetic distance from the CMV strains assigned to subgroup IA or subgroup IB. Infectious full-genome cDNA clones of CMV-209 were generated under the control of the Cauliflower mosaic virus 35S promoter. Infectivity of the CMV-209 clones was evaluated in Nicotiana benthamiana and various legume species. Our assays revealed that CMV-209 could systemically infect Glycine soja (wild soybean) and Pisum sativum (pea) as well as N. benthamiana, but not the other legume species.

• The Korean Journal of Ecology
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• v.15 no.3
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• pp.209-220
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• 1992

We investigated major plant communities and seed dispersal patterns in areas a(alt. 2,263 ~2,350m),b(alt. 2,350~2,420m), c(alt. 2,420m) with levels of a crater on mt. paektu. Thirty two species distributed around chunji consisted of 24 perennial herbs, 1 biennial herb and 7 alpine dwarf shurbs. Their fruit types were quite diverse; capsule(11), achene(10), grain(4), legume(2), follicle(2), schizocarp(2) and drupe(1). in area a, adjancent to the chunji(lake) water surface, gramineae sp., cyperaceae sp., chrysanthemum zawadskii and bistorta ochotensis were occured as members of the major plant communities. Species of gramineae and cyperaceae seem to have become dominant because their seeds float well. Astragalus membranaceus is legume and endures the winter cold and summer drought. in area c, alpine dwarf shrubs (e. g.rhododendron redowskianum, rhododendron aureum, empetrum nigrum) were dominant. In area c, compared to a and b, the weather is drier because of strong wind and lower water holding capacity of the soil. Therefore the pattern of plants distributing around chunji is different from that in western slope on mt.paektu. environmental factor such as wind, temperature and water may contribute to the pattern.

• 한국생태학회:학술대회논문집
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• 2002.08a
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• pp.93-100
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• 2002

Dynamics of certain $N_2$fixing bacteria such as Rhizobium, Azospirillum and Azotobactor, nodule number in dominant legume, Atylosia trinervia, P-solubilizing bacteria, actinomycetes and fungi were studied in unburned and burned site of natural grassland, southern India. Population of $N_2$- fixing bacteria, P-solubilizing bacteria, fungi and nodule number in legume increased significantly in burned sites. On the other hand, the actinomycetes population remained unchanged. Thirty six species of fungi with tricalcium phosphate solubilizing ability were recorded. The most efficient P-solubilizing fungi recognised in the soils of the study sites are Absidia ramosa, Gongronella butlerii, Mortieralla spinosa, Mucor racemosus, Rhizopus nigricans, R. stolonifer, R. oryzae, Aspergillus fumigatus, A. nidulans, A. niger Theilavia terricola and Cheatomium lunasporium.

• The Korean Journal of Ecology
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• v.25 no.4
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• pp.227-234
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• 2002

Dynamics of certain $N_2$ fixing bacteria such as Rhizobium, Azospirillum and Azotobactor, nodule number in dominant legume, Atylosia trinervia, P-solubilizing bacteria, actinomycetes and fungi were studied in unburned and burned site of natural grassland, southern India. Population of $N_2$ - fixing bacteria, P-solubilizing bacteria, fungi and nodule number in legume increased significantly in burned sites. On the other hand, the actino-mycetes population remained unchanged. Thirty six species of fungi with tricalcium phosphate solubilizing ability were recorded. The most efficient P-solubilizing fungi recognised in the soils of the study sites are Absidia ramosa, Gongronella butlerii, Mortieralla spinosa, Mucor racemosus, Rhizopus nigricans, R. stolonifer, R. oryzae, Aspergillus fumigatus, A. nidulans, A. niger, Theilavia terricola and Cheatomium lunasporium.