• Title/Summary/Keyword: lectin

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돼지의 체외수정시 투명대내 Lectin 결합과 수정촉진 Peptide의 영향

  • 황인선;정희태;양부근;김정익;박춘근
    • Proceedings of the KSAR Conference
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    • 2002.06a
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    • pp.13-13
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    • 2002
  • 수정촉진 peptide(Fertilization Promoting Peptide; FPP) 는 체내에서 정자-난자의 결합시 투명 대내에서 glycoprotein 과 progesterone에 의해 정자침입이 활성화 될 때까지 첨체반응을 억제함으로써 정자의 수정상태 유지를 위하여 필요한 물질로 알려져 있다. 한편, 정자내에 존재하는 lectin과 같은 단백질 및 효소 등은 투명대내에 존재하는 oligosaccharide 잔기를 합성시킨다. 본 연구는 돼지 정자-난자의 체외수정시 투명대내 FITC-labelled 처리된 lection의 결합과 FP의 영향을 검토하고자 수행되었다. (중략)

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Isolation and Carbohydrate Binding Specificity of a Lectin from the Hemolymph of Coastal Crab Macrophthalmus Japonicus (해양칠게 혈림프로부터 렉틴의 분리 및 당 결합특성)

  • Kim, Ha-Na;Chung, Woo-Hyuk;Bae, Chan-Hyung;Hwang, Kwang-Woo;Kim, Ha-Hyung
    • YAKHAK HOEJI
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    • v.50 no.3
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    • pp.166-171
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    • 2006
  • A calcium-dependent sialic acid-binding lectin has been isolated by thyroglobulin-affinity chromatography from the coastal crab Macrophthalmus Japonicus. This lectin, Macrophthalmus Japonicus lectin (MJL), was eluted with 50mM Tris-HCl, 0.3 M NaCl, 10 mM EDTA, and the recovery yield from the crude protein extract was about 5.6%. The molecular weight of MJL was estimated as 65 kDa in SDS-PAGE both under reducing and non-reducing conditions. MJL induced an agglutination reaction in rabbit, rat, and mouse erythrocytes, but not in human ABO types. This activity was effectively inhibited by sialoglycoproteins such as fetuin, bovine submaxillary mucin, and thyroglobulin.

Effect of Korean Mistletoe Lectin on Gene Expression Profile in Human T Lymphocytes: A Microarray Study

  • Lyu, Su-Yun;Park, Won-Bong
    • Biomolecules & Therapeutics
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    • v.18 no.4
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    • pp.411-419
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    • 2010
  • Korean mistletoe has a variety of biological effects, such as immunoadjuvant activities. This study investigates the effects of Korean mistletoe lectin (Viscum album L. var. coloratum agglutinin, VCA) on human T lymphocytes to determine whether VCA acts as an immunomodulator. Purified human T-lymphocytes were cultured with VCA and RNA from each point was analyzed using Affymetrix human genome chips containing 22,500 probe sets which represents more than 18,000 transcripts derived from 14,500 human genes. As a result, there was a striking upregulation of genes coding for chemokines. Seventeen genes out of 50 coding for proteins with chemokine activity were upregulated including CXCL9 and IL-8 which are related to the treatment of cancer. In addition, 28 cytokine genes were upregulated including IL-1, IL-6, IL-8, IFN-$\gamma$, and TNF-$\alpha$. Taken together, the data suggest that Korean mistletoe lectin, in parallel with European mistletoe, has an ability to modulate human T cell function.

Assay Method for Lectin-conjugated Ellagitannin Encapsulated in Liposomal Formulations (리포좀 제제 중 렉틴-엘라지탄닌 포합체의 분석법 확립)

  • Jeon, Hyun-Joo;Choi, Young-Wook
    • Journal of Pharmaceutical Investigation
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    • v.31 no.3
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    • pp.197-200
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    • 2001
  • Lectin-conjugated ellagitannin (LET), a newly introduced melanoma-specific antitumor agent which has been synthesized by conjugation of wheat germ agglutinin as a lectin with praecoxin A as an ellagitannin, was encapsulated into sterically stabilized liposomes (SSL). Modified Folin phenol method was established for the quantitation of LET contents in liposomal formulations protein employing the standard calibration curve with bovine serum albumin. After removal of phospholipid by organic solvent extraction, which interferes the specific selectivity of the Folin-Ciocalteu reagent with the protein, recovery of LET was $94.5{\pm}2.3%$ and the encapsulation efficiency was revealed as $37.8{\pm}5.9%$ for 2.5 mg/ml LET solution.

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Isolation and Characterization of Lectin from Viscum coloratum (겨우사리 중의 렉틴성분 분리 및 특성)

  • Park, Won-Bong;Kim, Hee-Sook
    • YAKHAK HOEJI
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    • v.38 no.4
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    • pp.418-424
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    • 1994
  • Lectin from mistletoe (Viscum coloratum) were obtained by salt fractionation, gel filtration and anion exchange chromatography. A molecular weight of about 60,000D has been determined by SDS-PAGE and two basic subunits which have molecular weights of 33,000D and 28,000D are linked by a disulfide bond. The partially purified mistletoe lectins agglutinated human B erythrocytes. Agglutinating activity was relatively stable at varied pH $(3.77{\sim}8.71)$ and at temperature range of $0{\sim}40^{\circ}C$ and not affected by 9 metal ions. Galactose, lactose and N-acetyl-D- galactosamine inhibited agglutinating activity of lectin. Lectin from mistletoe was more mitogenic to murine lymphocytes than concanavalin A.

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Identification of Cochlodinium polykrikoides against Gyrodinium impudicum and Gymnodinium catenatum in Field Samples using FITC Lectin Probes

  • Cho Eun Seob;Kang Dong Woo;Cho Yong Chul
    • Fisheries and Aquatic Sciences
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    • v.3 no.2
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    • pp.83-87
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    • 2000
  • We have investigated lectin binding patterns in order to apply binding records of previous laboratory experiments to field settings before the first ourbreaks of harmful algal bloom (HAB). Although cells were grown under different conditions, the binding patterns were the same as in the control. In addition, culture days was not associated with the binding patterns, when compared with the control. In nature, this results suggest that ECA, HPA and WGA lectin are able to discriminate between C. polykrikoides and G. impudicum, as well as ECA and SBA have a capability as a tool for differentiating between C. polyrikoides and G. catenatum, although these species are closely similar under the light microscope fiexed with Lugol solution.

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Identification of Species-Specific Components between Hanwoo and Holstein Meat (한우 및 홀스타인육의 품종간 특이성분의 검색에 관한 연구)

  • 황보식;이수원;임태진;정구용
    • Food Science of Animal Resources
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    • v.21 no.3
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    • pp.246-255
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    • 2001
  • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of muscles extracted with distilled water, saline solution, SDS or Trition X-100 showed simular protein patterns between Hanwoo and Holstein meat, indicating that SDS-PAGE technique may not be useful for the identification between Hanwoo and Holstein meat. Lectine blot analysis of muscle extracted with distilled water demonstrated that Hanwoo and Holstein meat had similar affinities for concanavalin A (Con A), ricinus communis agglutinin (RCA-120), ulex europaeus agglutinin (UEA-1) or peanut agglutinin (PNA) lectins. However, approximately 32.1 kDa component of Hanwoo meat showed high affinity for dolichos biflorus agglutinin (DBA) lectin. On the contrary, high molecular weight components of Holstein meat had the specific affinity for wheat germ agglutinin (WGA) lectin. Hanwoo meat-specific components were observed by lectin staining of heat-denatured meat at 100$^{\circ}C$ for 30 sec. Also, the component of heat-denatured meat at 100$^{\circ}C$ for 30 sec, which was slightly smaller than Hanwoo meat-specific component, was concentrated specifically in Holstein meat.

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Molecular Cloning and Expression of Rice Lectin in Escherichia coli (벼 렉틴 유전자의 클로닝 및 대장균에서의 발현)

  • 홍성관;전상훈;김하형;공광훈
    • YAKHAK HOEJI
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    • v.46 no.4
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    • pp.270-275
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    • 2002
  • The lectin gene from rice was amplified by the polymerase chain reaction. The amplified DNA was inserted into the expression vector pET26b and expressed it as a fusion protein with polyhistidine sequences in Escherichia coli. The recombinant protein was produced by induction with 0.4 mM isopropyl-$\beta$-D-thiogalactopyranoside at 37$^{\circ}C$ and purified by an immobilized metal affinity chromatography. The recombinant protein was found to have lectin activity by the hemagglutination inhibition assay. The hemagglutination activity of the recombinant protein was optimal at pH 4.0-7.0 and was dependent on $Ca^{2+}$ and Mn$^{2+}$.2+/.

Analysis of Erythropoietin Glycoform Produced by Recombinant CHO Cells Using the Lectin-Blotting Technique

  • Chang, Kern-Hee;Kim, Kyung-Soo;Kim, Jung-Hoe
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.3 no.1
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    • pp.40-43
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    • 1998
  • The glycosylation pattern of Erythropoietin (EPO), produced by recombinant CHO cells, was studied using the simple and rapid technique of 'Lectin-blotting'. In this experiment we used three different kinds of lectins, MAA(Maackia amurensis agglutinine), RCA(Ricinus communis agglutinine), and DSA(Datura stramonium agglutinine), which bind to the terminal sialic acid, galactose, and the N-acetyllactosamine chain respectively. The lectin-blotting technique was used to analyze the carbohydrate structure of EPO produced in the presence of two physiologically active chemical compounds, ammonium and chloroquine. The effect of the ammonium ion on the glycosylation of EPO was studied because it accumulated in the medium mainly as a by-product of glutamine matabolism. Ammonium chloride significantly inhibited the sialylation of the terminal galactose residue at concentrations of 8mM or more. Chloroquine, a potent inhibitor of glycosylation, inhibited terminal sialylation at concentrations of 100 and 200 $\mu$M, and at a concentration of 300 $\mu$M, also inhibited Nacetyllactosamine chain synthesis.

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Lectin histochemistry of lung tissues in the Streptozotocin rat fetus (Streptozotocin을 투여한 흰쥐 태자 폐조직의 렉틴 조직화학 염색성)

  • Hong, Hea-Nam;Kim, Dong-Hou
    • Applied Microscopy
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    • v.23 no.2
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    • pp.84-96
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    • 1993
  • This study was to investigate the effects of maternal diabetes on the lung tissue of the fetal rat using lectin histochemistry and electron microscope technique. Maternal diabetes was induced by intraperitoneal injection of streptozotocin (75 mg/kg the body weight) into pregnant Sprague-Dawley rats on the 7th day of gestation. Fetuses of streptozotocin induced diabetic rats exhibited delayed lung maturation and reduced air space. In lectin histochemistry, the binding of Maclura pomifera (MPA) to fetal lungs from diabetic mothers was reduced, but no significant changes in the bindings of Concanavalin A (Con A), Wheat germ agglutinin (WGA), Ricinus communis I (RCA I) and Griffonia simplicifolia (GSI-$B_4$) were noted. Because the MPA has affinity to terminal N-acetyl-D-galactosamine residues constantly linked O-glycosidically to serine or threonine, the present findings may indicates that maternal diabetes interfere with the processes of O-linked glycosylation in fetal rat lung.

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