• KSBB Journal
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• v.17 no.4
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• pp.357-364
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• 2002

For the effecient extraction methods of essential oil, pretreatment of leaves, ratios of water and leaves, extraction time, and collection season from the Chamaecyparis obtusa and Chamaecyparis pisifera leaves were studied. The chemical composition of essential oil was analyzed by GC-MS. The yield of essential oil from ground leaves was higher than that of chopped leaves. The yield of essential oil was not affected much by mixing ratios of water and leaves. The yield of essential oil reached maximum after 5 hours. The content of essential oil of C. obtusa leaves collected during winter was 4.5%, whereas the content of essential oil of C. pisifera collected during fall was 5.3%. The composition of essential oils extracted form C. obrusa and C. pisifera was different. The major constituents in the essential oil of C. obtusa were monoterpene as limonene, terpinene-4-ol, ${\gamma}$-selinene, and a-cedrene, and those of C. pisifera was monoterpens as ${\alpha}$-pinene, myrcene, limonene, bornyl acetate, ${\beta}$- caryophyllene, longifolene, and ${\beta}$-cedrene.

• Korean Journal of Agricultural Science
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• v.42 no.2
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• pp.111-116
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• 2015

Combined applications of rice bran with pine leaves were tested to examine the inhibitory effects to paddy weeds and increased yield of rice for developing techniques of environment-friendly weed management in paddy rice fields. Weed control efficacy at 60 days after treatment was improved to 88.8% by combined application of rice bran with pine leaves, while weed control efficacy by single application of rice bran showed 67.5%. The other weed control efficacy combined with chestnut leaves and barley straws were 76.3% and 69.9% respectively. Combined application of rice bran with pine leaves was more effective to broadleaf weeds such as Monochoria vaginalis. Weed control efficacy by combined application with pine leaves was 100% until 70 days after rice transplanting and maintained weed control effect up to 90 days after transplanting. Rice yield by combined application of rice bran with pine leaves was 526 kg/10 a, which was higher yield than by combined application with barley straws, single application of rice bran, natural snail application and hand weeding.

• Korean Journal of Agricultural Science
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• v.41 no.3
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• pp.169-175
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• 2014

Objective of this study was to investigate effects of pre-processing method and number of sampling leaves on stability of the reflectance measurement for Chinese cabbage and kale leaves. Chinese cabbage and kale were transplanted and cultivated in a plant factory. Leaf samples of the kale and cabbage were collected at 4 weeks after transplanting of the seedlings. Spectra data were collected with an UV/VIS/NIR spectrometer in the wavelength region from 190 to 1130 nm. All leaves (mature and young leaves) were measured on 9 and 12 points in the blade part in the upper area for kale and cabbage leaves, respectively. To reduce the spectral noise, the raw spectral data were preprocessed by different methods: i) moving average, ii) Savitzky-Golay filter, iii) local regression using weighted linear least squares and a $1^{st}$ degree polynomial model (lowess), iv) local regression using weighted linear least squares and a $2^{nd}$ degree polynomial model (loess), v) a robust version of 'lowess', vi) a robust version of 'loess', with 7, 11, 15 smoothing points. Effects of number of sampling leaves were investigated by reflectance difference (RD) and cross-correlation (CC) methods. Results indicated that the contribution of the spectral data collected at 4 sampling leaves were good for both of the crops for reflectance measurement that does not change stability of measurement much. Furthermore, moving average method with 11 smoothing points was believed to provide reliable pre-processed data for further analysis.

• Korean Journal of Medicinal Crop Science
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• v.11 no.1
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• pp.40-45
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• 2003

Volatile components in the leaves and fruits of Z. schinjfolium and Z. piperitum were analyzed by Headspace SPME(Solid phase Microextraction). Fifty two and 48 components in the leaves and fruits, repectively, were identified in Z. schinifolium. (E)-2-hexenal, ${\alpha}-pinene$, (Z)-ocimene+limonene, estragole, germacrene-d were detected at common components in the leaves and estragole in the fruits of Z. schinjfolium. Regardless of collection sites hexanal, (Z)-3-hexenol, (E)-2-hexenal, n-hexanol were appeared in the leaves while undecanone in the fruits. Thirty and 27 components in the leaves and fruits, respectively, were identified in Z. piperitum. ${\alpha}-pinene,\;{\beta}-phellandrene$, 1,8-cineole, citronellal and myrcene, (Z)-ocimene+limonene, ${\beta}-phellandrene$ were appeared as common components in the leaves and fruits collected from Baeck-yang-sa and Nae-jang-sa. (Z)-3-hexenol, (E)-2-hexenal, ${\alpha}-pinene\;myrcene\;and\;{\beta}-phellandrene$, citronellal, geranyl acetate were major components in the leaves and fruits from Tong-do-sa.

• Journal of Life Science
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• v.23 no.11
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• pp.1381-1387
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• 2013

The leaves and roots of Ligularia stenocephala, which are widely used as a food in Korea, were investigated for their antioxidant activities and cytotoxicity in vitro, and their hepatoprotective effect, alcohol detoxicant efficacy, and memory-enhancing property were investigated in vivo. The unique odor of the leaves was analyzed by GC-MS. Lipid peroxidation, superoxide anion formation, and DPPH radicals were inhibited remarkably by the extracts of the leaves and roots. The leaves of this edible plant significantly protected the hepatotoxicity induced by carbon tetrachloride and further diminished the blood alcohol content in mice. While the roots of this plant exhibited adequate cytotoxicity against four human tumor cell lines, especially against melanoma, the leaves revealed relatively weak activity. Both the leaves and the roots exerted an excellent ameliorating property on scopolamine-induced memory impairment in the passive avoidance task using an animal model. The hexane fraction of the leaves was analyzed by GC-MS, suggesting that a series of terpenoids may be odorous compounds in this plant.

• Journal of The Korean Society of Grassland and Forage Science
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• v.34 no.1
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• pp.15-20
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• 2014

To investigate lignification process and its physiological significance under water-deficit condition, the responses of peroxidases, polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL) in relation to leaf water status to the short term of water deficit treatment in the leaves with different maturities in forage rape were measured. The significant decrease in relative water content (RWC) and leaf osmotic potential (${\Psi}{\pi}$) were apparent after 5 d of water-deficit treatment. The activity of guaiacol peroxidase (GPOD), ascorbate peroxidase (APOD), coniferyl alcohol peroxidase (CPOD), and syringaldazine peroxidase (SPOD) was depressed especially in middle and old leaves when compared with that of control leaves. On the other hand, in young leaves, a significant increase in CPOD (+34%) and SPOD (+24%) activity as affected by water-deficit treatment was apparent. The activation of PAL and PPO was observed in middle and old leaves for PAL and in young and middle leaves for PPO. These results suggest that peroxidases in middle and old leaves did not involve in lignification under mild water-deficit stress, whereas CPOD and SPOD in young leaves participate in lignification by a coordination with PAL and PPO to incorporate phenol and lignin into the cell walls.

• The Korean Journal of Food And Nutrition
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• v.28 no.2
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• pp.295-301
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• 2015

Biological compounds such as crude saponin and total polyphenol were determined in Allium hookeri roots and leaves. In addition, activities of DPPH radical scavenging activity, hydroxyl radical scavenging activity, ${\alpha}$-amylase inhibition activity and nitrite scavenging were detected in concentrates of 70% ethanol extracts of Allium hookeri roots and leaves. The crude saponin content of the roots and leaves were 4.28 mg/g, and 4.17 mg/g, respectively. Total polyphenol and total flavonoid content was higher in leaves than roots. Furthermore the DPPH radical scavenging activity and hydroxyl radical scavenging activities of leaves was higher than roots. For ${\alpha}$-glucosidase inhibitory activity, 70% ethanol extracts of Allium hookeri roots and leaves showed 60% activity inhibition at a 10 mg/mL concentration. Nitrite scavenging abilities under acidic conditions were most effective for Allium hookeri leaves extracts. These results suggested that Allium hookeri leaves might be used as the components of health functional food.

• The Korea Journal of Herbology
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• v.31 no.6
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• pp.21-28
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• 2016

Objectives : This study was performed to investigate the antioxidant activity of water extracts from Lycopus lucidus Turcz. ex Benth. leaves, stems and roots at the $100{\mu}g/m{\ell}$ concentration. Methods : The different part of Lycopus lucidus Turcz. ex Benth. extract was prepared using water. The antioxidant activities of polyphenol contents, total flavonoid contents, DPPH(1,1-diphenyl-2-picrylhydrazyl) free-radical scavenging activity, SOD like activity, hydroxyl radical, ABTS(2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), $Fe^{2+}$ chelating, and nitrite scavenging activity. Results : The total polyphenol and total flavonoid content of leaves were the highest at $221.85{\mu}g/mg$ and $794.13{\mu}g/mg$, respectively. Electron donating ability was the 79.68% in the water extract from leaves. The ABTS radical scavenging activity of the hot extracts, leaves ${\gg}$ roots > stems was higher in the order. It was shown the highest at 94.53% in the water extract from leaves, which showed a value equal to 94.7% of ascorbic acid. The hydroxyl radical scavenging activity was the highest at 8.07% in the water extract from leaves. SOD like activity and $Fe^{2+}$chelating activity were leaves of 12.3% and 27%, respectively, which were much higher than those of any other parts. The nitrite scavenging ability of extracts was increased at pH 2.5, and those was the highest in leaves of 83.03%. Its were more than twice the 41.61% of BHT. Conclusion : The results suggest that Lycopus lucidus Turcz. ex Benth. can be used as nutraceutical foods and natural antioxidant.

• The Korea Journal of Herbology
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• v.31 no.6
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• pp.45-52
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• 2016

Objectives : This study was performed to investigate the antioxidant activity of water and ethanol extracts from Sasa borealis leaves, stems and roots. Methods : Sasa borealis leaves, stems and roots extract were prepared using water and ethanol. The total polyphenol and total flavonoid contents were analyzed. 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical scavenging activity, SOD like activity, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)(ABTS), hydroxyl radical scavenging activity, and Nitrite scavenging activity assays were carried out to determine the antioxidant activities. Results : The antioxidant activities of the Sasa borealis appeared higher in ethanol extract than water extracts. Total polyphenol and total flavonoids contents in ethanol extracts of leaves were $24.6mg/m{\ell}$ and $14.3mg/m{\ell}$, respectively, which were much higher than those of any other parts. SOD like activity was 70% ethanol extract of the leaves was highest with 15.68%. Electron donating ability was 70% ethanol extract of the leaves had the highest 59.07%. It exhibited high electron donating ability than BHT(45.68%). Nitrite scavenging activity of 70% ethanol extract was higher than the water extract at pH 2.5 and pH 4.2. Nitrite scavenging activity was 70% ethanol extract of the leaves was the highest 75.2%. Hydroxyl radical scavenging activity was 70% ethanol extract of the leaves was highest with 16.16%, showed very low activity than BHT(61.56%). Conclusions : These results suggest that 70% ethanol extracts from Sasa borealis leaves, exhibited higher antioxidant activities than those of root and stem, and can be potentially used as proper natural antioxidants.

• Advances in environmental research
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• v.12 no.1
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• pp.51-64
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• 2023

The sustainable management of rice straw is essential for protection of human health and environment. This study assesses the impact of stock density of earthworm (Eisenia fetida) and Neem leaves (Azadirachta indica) on the quality of the final vermicompost. The vermicompost is produced using different combinations of rice straw, Neem leaves, and cow dung (bulking agent) by varying stock density of earthworms. The vermicomposting experiments are performed in plastic containers (32 cm × 28 cm × 28 cm) in open for 90 days under laboratory conditions. The stock density of the earthworm is found to be an important factor to influence nutritional quality of the final vermicompost. There is observed significant improvement in the total nitrogen (91.8%), phosphate (73.4%), potassium (38.8%), and calcium (59.05%) content of the vermicompost produced with the highest stock density of the earthworms. All the treatments showed decrease in TOC and C:N content after 90 days of vermicomposting. The treatment with Neem leaves showed maximum growth of earthworms (2.65 fold). Neem leaves brought positive changes in the quality of final vermicompost by enhancing the growth and reproduction of the earthworms. The calcium content increased by 39% in the final vermicompost with the addition of Neem leaves at the same stock density of the earthworms. The stock density of the earthworms and Neem leaves are found to significantly improve quality of the final vermicompost as compared with the compost (control). The surface morphology in SEM images showed high degree of fragmentation in the vermicompost as compared with the compost. The combined action of microbes and earthworms resulted in high degree of disintegration in the vermicompost.