• 한국작물학회지
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• 제49권4호
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• pp.331-336
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• 2004

Changes in the level of metabolites in leaves and pods were examined with respect to the seed chemical composition in black soybean. There was no further increase in pod length after 42 days after flowering (DAF). Pod weight, however, persistently increase until 73 DAF, thereafter the weight was slightly lowered. The seed storage protein, however, increased drastically as the increasing rate of pod weight was lessened at 61 DAF. The accumulation of seed storage proteins was occurred conspicuously as the increasing rate of pod weight was slowed down. The chlorophyll content both in leaves and pods was drastically decreased after 50 DAF. The beginning of drastic reduction in chlorophyll content was occurred concomitantly with the reduction of soluble protein content in leaves. The sugar content in leaves showed similar tendency with chlorophyll and soluble protein content. The starch level in leaves, however, showed different changing pattern during seed development. The starch content in leaves was increased persistently until 66 DAF, thereafter the content was decreased drastically to about $55\%$ of maximal value at 66 DAF. Total phenolics content in leaves and the anthocyanins content in seeds were stable without noticeable increase until 66 DAF. The contents were increased dramatically after 66 DAF showing the synchronized pattern with the decrease in starch level in leaves. The levels of the selected metabolites in leaf and seed suggested that the accumulation of chemical components of black soybean seed is launched actively at 66 DAF. The profile of storage proteins was nearly completed at 61 DAF because there was no large difference in densitometric intensity among protein subunits after 61 DAF. In soybean, chemical maturation of seed begins around 61 to 66 DAF at which most metabolites in vegetative parts are decreased and remobilized into maturing seeds.

• 원예과학기술지
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• 제31권2호
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• pp.231-238
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• 2013

Perilla frutescens, which comprises var. frutescens and var. crispa, has been cultivated traditionally in Asian countries as an edible oil, leaf vegetable, and medicinal crop. To evaluate the lipophilic phytonutrient properties of P. frutescens, we selected 54 Perilla accessions [19 landraces of var. frutescens (FL), 22 weedy type var. frutescens (FW), 9 weedy type var. crispa (CW), 2 cultivars of var. frutescens widely cultivated for seed oil (FCS), and 2 cultivars of var. frutescens cultivated as a leaf vegetable (FCL)] and analyzed their seeds and leaves for vitamin E, squalene, and phytosterols. Among the four vitamin E isomers analyzed, ${\gamma}$-tocopherol was the major form of vitamin E in seeds, whereas ${\alpha}$-tocopherol was the major form in leaves of all types of P. frutescens. The highest total vitamin E content in seeds was present in FL ($170.0mg{\cdot}kg^{-1}$), whereas that in leaves was highest in FCL ($358.1mg{\cdot}kg^{-1}$). The highest levels of squalene in seeds and leaves were in FL ($65.5mg{\cdot}kg^{-1}$) and CW ($719.3mg{\cdot}kg^{-1}$), respectively. Among the three phytosterols, ${\beta}$-sitosterol occurred in the highest amount in both leaves and seeds of all of the crop types. Phytonutrient contents were comparatively higher in leaves than in seeds of all crop types. All of these results suggest that the consumption of leaves and seeds of Perilla crops could be beneficial to human health, as Perilla possesses considerable amounts of various lipophilic compounds.

• The Plant Pathology Journal
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• 제21권3호
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• pp.237-243
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• 2005

Resistance inductions on the leaves of cucumber plant by an arbuscular mycorrhiza Glomus intraradices were investigated. In addition, the infection structures were observed at the penetration sites on the leaves of plant inoculated with Colletotrichum orbiculare using a fluorescence microscope. The severity of anthracnose disease caused by Colletotrichum orbiculare was significantly decreased on the leaves of cucumber plant colonized with G intraradices compared with those of non-treated control plants. As a positive control, pre-treatment with DL-3-aminobutyric acid (BABA) caused a remarkable reduction of the disease severity on the pathogen-inoculated leaves. There were no significant differences in the frequency of either germination or appressorium formation of the plant pathogen between mycorrhiza colonized and non-treated plants. It was also the same on the BABA pre-treated plants. However, the frequency of callose formation was significantly high on the leaves of G intraradices colonized plants compared to those of non-treated control plants at 5 days after challenge inoculation. On the leaves of BABA treated plants callose formation was not significantly high than those of non-treated, although the disease severity was more strongly suppressed. It was suggested that the resistance induced by colonization with G. intraradices might be related to the enhancement of callose formation at the penetrate sites on the leaves invaded by the pathogen, whereas resistance by BABA did not.

• Asian-Australasian Journal of Animal Sciences
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• 제24권8호
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• pp.1157-1163
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• 2011

This study was conducted to determine the effect of the inclusion of different levels of ensiled cassava leaves (variety KM94) in the diets on performance and carcass characteristics of growing pigs in Vietnam. A total of 40 crossbreds pigs (Large White${\times}$Mong Cai, 20 males and 20 females) with an initial live weight of 23.5 kg (SD = 0.86) were randomly allocated to one of the four pens across 5 units. Four experimental diets were formulated for two growth periods, period 1 (60 days) for 20 to 50 kg and period 2 lasted 30 days, from 50 kg until slaughter. Four diets were formulated containing inclusion levels of ensiled cassava KM94 leaves diet of 0, 10, 15 and 20% in the DM. Diets were formulated based on previously determined ileal amino acid digestibility values of the KM94 products and were isonitrogenous and isocaloric on a metabolizable energy basis. Each pen of pigs was randomly assigned to one of the four dietary treatments. Dry matter intake and final weight tended to decreased with increasing levels of ensiled cassava KM94 leaves in the diet while there was a significant (p = 0.022) decrease in average daily gain. Protein depositions of the F1 pigs tended (p = 0.093) to decrease with increasing inclusion levels of ensiled cassava KM94 leaves. There was no significant difference in feed conversion ratio, carcass quality and fat gain between the groups of pigs. There were clear differences in feed costs among the experimental diets (p = 0.001) with increasing levels of ensiled cassava KM 94 leaves in the diet reducing feed costs. It was concluded that, in diets for growing pig, inclusion of ensiled cassava leaves reduces growth rate of pigs in Vietnam but increases profitability as measured by feed costs.

• Mycobiology
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• 제29권1호
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• pp.19-26
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• 2001

Defense mechanisms against anthracnose disease caused by Colletotrichum orbiculare on the leaf surface of cucumber plants after pre-treatment with plant growth promoting rhizobacteria(PGPR), amino salicylic acid(ASA) or C. orbiculare were compared using a fluorescence microscope. Induced systemic resistance was mediated by the pre-inoculation in the root system with PGPR strain Bacillus amylolquefaciens EXTN-1 that showed direct antifungal activity to C. gloeosporioides and C. orbiculare. Also, systemic acquired resistance was triggered by the pre-treatments on the bottom leaves with amino salicylic acid or conidial suspension of C. orbiculare. The protection values on the leaves expressing SAR were higher compared to those expressing ISR. After pre-inoculation with PGPR strains no change of the plants was found in phenotype, while necrosis or hypersensitive reaction(HR) was observed on the leaves of plants pre-treated with ASA or the pathogen. After challenge inoculation, inhibition of fungal growth was observed on the leaves expressing both ISR and SAR. HR was frequently observed at the penetration sites of both resistance-expressing leaves. Appressorium formation was dramatically reduced on the leaves of plants pre-treated with ASA, whereas EXTN-1 did not suppress the appressorium formation. ASA also more strongly inhibited the conidial germination than EXTN-1. Conversely, EXTN-1 significantly increased the frequency of callose formation at the penetration sites, but ASA did not. The defense mechanisms induced by C. orbiculare were similar to those by ASA. Based on these results it is suggested that resistance mechanisms on the leaf surface was different between on the cucumber leaves expressing ISR and SAR, resulting in the different protection values.

• 한국연초학회지
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• 제17권2호
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• pp.126-138
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• 1995

To investigate the changes of physicochemical properties of leaf tobacco during storage, cured leaves (both flue-cured(KF109) and burley(Burley 21) : 4 grades of leaves) were stored under the natural warehouse condition(room temperature) and processed leaves(both Rue-cured(NC82) and burly(Burley 21) : 6 grades of leaves) were stored at storerooms in tobacco processing plants(flue-cured Cheongju plant ; 2nd, 4th and 5th floor, burley : Kwangju plant ; 2nd floor, Chonju plant : 3rd and 5th floor). Tobacco leaves were sampled and analyzed every 3 months. Total sugar content of flue-cured leaf decreased slightly and the redness degree of leaf increased after 15 months' storage under the natural warehouse conditions. The pH of cured leaves were lowered both flue-cured and burley, and the decreasing rate of pH was large in flue-cured(0.24) as compared with burley(0.14). There was no significant differences of physicochemical properties of processed leaf among storerooms during 15 months' storage. The decreasing rate of processed leaf pH was somewhat large in flue-cured(0.26) as compared with burly (0.20), and in thick leaves as compared with thin leaves. The redness degree of flue-cured leaf increased slightly, while the degree of lightness and yellowness lowered slightly during storage. The lightness degree of burley leaf lowered slightly, too. The ageing process of cured leaf was similar to that of processed leaf, it is considered that the passing days after curing will be more reasonable than the passing days after processing for the establishment of proper ageing period.

• 한국화재소방학회논문지
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• 제22권1호
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• pp.84-92
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• 2008

본 연구에서는 영동지역의 대표 수종인 소나무와 떡갈나무를 대상으로 주요 부위별 열적특성을 고찰하고자 콘칼로리미터 시험을 수행하여 중량변화, 착화시간, 화염유지시간, 발열량 그리고 CO와 $CO_2$ 배출농도를 비교 분석하였다. 소나무와 떡갈나무 모두 가지의 총중량감소가 가장 크게 나타났으며, 낙엽의 착화시간이 9 s 정도로서 가장 빠르게 나타났다. 낙엽과 수피부위의 화염유지시간은 $640{\sim}1,016s$ 정도였으며, 총방출열량은 낙엽비 생엽의 2배 정도인 60.1 $MJ/m^2$로 가장 높게 나타났다. 또한, CO 및 $CO_2$의 최대 배출농도는 떡갈나무의 가지부위가 소나무의 가지보다 2.82배 이상 높게 나타남으로써 소나무와 떡갈나무의 열적특성은 부위별로 많은 차이가 있음을 확인하였다.

• 한국약용작물학회지
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• 제22권1호
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• pp.8-16
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• 2014

The present study was carried out to evaluate the antioxidant and anti-inflammatory activities of three extracts (hot water, 50% ethanol and mixed solvent;water, ethanol, butylene glycol, propylene glycol) of dried chestnut, chestnut shell, chestnut leaves and dried chestnut leaves obtained from Castanea crenata tree. When conducted DPPH assay, radical scavenging activity of ethanol extract of chestnut shell was the highest with $IC_{50}$ $10.8{\mu}g/mL$ among four extracts from these parts (p < 0.05). In additional results by the xanthine oxidase assay, antioxidant activity showed that water extract of chestnut leaves showed the highest xanthine oxidase inhibitory activity in the tested extracts (p < 0.05). Futhermore, extracts of chestnut shell and leaves exhibited no cytotoxicity in RAW 264.7 cells (p < 0.05). Also, anti-inflammatory activity by NO assay showed LPS-induced NO was significantly inhibited following treatment with extracts of chestnut shell and leaves of 3mg/mL (p < 0.05). These data suggest that extract of chestnut shell have antioxidant and anti-inflamantory activity including chestnut leaves. Therefore, it is considered that Castanea crenata research range and selection of functional material can broaden chestnut shell to other fractions such as chestnut and chestnut leaves.

• 한국작물학회지
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• 제40권3호
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• pp.359-364
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• 1995

논담배 품질저하 요인으로 알려져 있는 grey엽발생원인 구명의 기초자료로 이용하기 위하여 논토양에서 자연발생된 grey엽을 채취, 피해정도별로 색채와 화학성분적 특성을 조사 분석한 결과는 다음과 같다. 1. Grey엽의 발생엽수와 발생율은 하위엽에서 가장 많았고 중 > 본 > 상엽 순이었으며 엽중성분함량은 정상엽에 비하여 Fe, Mn, Cl함량이 놓았고 피해증상이 심할수록 높았다. 2. 동일엽내에서 grey피해부위가 정상부위에 비하여 Fe, Mn, Cl함량이 높고 전질소, 에테르추출물은 낮았으며, 니코틴, 전당, Ca함량에서는 차이가 없었다. 3. Grey엽의 내용성분간에는 전질소와 Cl, Fe와 Mn간에 정상관이, 전질소와 전당 및 Mn, Cl과 전당간에는 각각 부의 상관관계가 있었다. 4. 색상은 정상엽에 비하여 증상이 심할수록 L치가 크게 감소하고 b치, a치 순으로 감소하였으며, L치와 a치는 일정한 경향이 없었으나 b치는 현저히 낮아 grey엽의 특성으로 나타났다.

• 한국농림기상학회지
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• 제7권4호
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• pp.250-257
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• 2005

본 연구는 동일 포장 내 부유와 서촌조생 품종의 잎 내 무기성분의 시기별 함량 변화와 기상요인을 조사하였다. '부유'와 '서초조생' 잎의 N, P, K, 및 Cu 함량은 후기로 갈수록 감소되었으나, Ca, Mg, 및 Mn 함량은 증가되었다. 그리고 '부유' 잎의 K함량은 서촌조생' 보다 높았으나, '서촌조생' 잎의 P와 Ca 함량은 '부유' 보다 높았다. 평균기온과 K 함량은 정의 상관이 인정되었다. 강우량은 Ca 함량과는 정의 상관이, N과 Cu 함량과는 부의 상관이 인정되었다. 그리고 일조량은 N과 Cu 함량과는 정의 상관이, Ca, Mg 및 Mn과는 부의 상관이 인정되었다.