• KSCE Journal of Civil and Environmental Engineering Research
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• v.36 no.5
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• pp.769-777
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• 2016

The objective of this study was to evaluate the stream depletion from groundwater pumping in deep aquifer using the Ward and Lough's analytical solution (2011) which considers a two-layer leaky aquifer system. The calculated results for each pumping from the 110 wells beside streams showed a wide range of non-dimensional stream depletion, that is the streamflow depletion rate divided by the groundwater pumping rate, from lower than 0.1 to more than 0.9 on average for 5 years depending on the specific properties of well location. From the comparison with Hunt's solution (1999) of a single layer aquifer, the Ward and Lough's solution showed about 50% lower than the Hunt's solution due to the difference of hydraulic properties between the first and second layers as well as the lagged effect of vertical leakance. It was also found that the groundwater pumping has a minor effect on the stream depletion if the stream depletion factor (SDF) of the 1st layer is higher than about 1,000 or the SDF of the 2nd layer is higher than about 100, or the vertical leakance is smaller than $10^{-5}s^{-1}$. Furthermore, in the present study, the variations of the stream depletion were assessed according to the magnitude of unmeasured hydraulic properties such as transmissivity and storage coefficient of the 1st layer, vertical hydraulic conductivity of the 2nd layer, the streambed hydraulic conductance.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.40 no.4
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• pp.383-391
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• 2020

In this study, Hunt's analytical solution and Ward & Lough's analytical solution for two-layered leaky aquifer system were used to estimate stream depletions due to shallow and deep groundwater pumping, and their differences were compared. Depending on the combination of the separation distance between the stream and the well, the transmissivity and the storage coefficient of the aquifer, and the leakage coefficient between the upper and lower layers, the stream depletion, which is the amount of stream water reduction compared to the amount of groundwater pumping, for each of 45,000 cases was calculated for both shallow and deep groundwater pumping, and the differences were analyzed quantitatively. When the leakage coefficient was very small, with a value of 10^-61/d, the difference in the average five-year stream depletion due to the pumping of shallow and deep groundwater showed a large deviation of up to 0.9 depending on the given hydraulic characteristics; this value exponentially decreased as the stream depletion factor (SDF) increased. This exponential relationship gradually weakened as the leakage coefficient increased due to interaction effects between layers, resulting in a small difference of up to 0.2 when the leakage coefficient reached 10^-31/d. Under the condition of greater interlayer hydraulic connectivity, there was little influence of the depth of groundwater pumping on the stream water reduction.

• Journal of Life Science
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• v.17 no.9 s.89
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• pp.1298-1302
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• 2007

A hallmark of cancers is 'leaky' tight junctions (Tjs). TJs mediated paracellular permeability is elevated and TJs maintained cell polarity is frequently lost. Concomitantly, TJs-associated proteins including members of the claudin family of proteins are dysregulated. Recent findings indicate that these TJs changes can contribute to cancer progression. In this study, we examined the effects of ${\beta}-lapachone$, a quinone compound obtained from the bark of the lapacho tree (Tabebuia avellanedae), on the Tjs-associated regulators in human hepatocarcinoma cell lines, HepG2 and Hep3B. ${\beta}-lapachone$ treatment downregulated the levels of insulin-like growth factor 1 receptor (IGF-lR) proteins in both HepG2 and Hep3B cells. But the levels of claudin-3 and -4 proteins were increased in ${\beta}-lapachone$-treated HepG2 and Hep3B cells. And also the zonnula occludens-l (la-I) and p-catenin protein levels by ${\beta}-lapachone$ were increased in a time-dependent manner. However, claudin-3 and -4 mRNA levels were uninhibited by ${\beta}-lapachone$ in HepG2 and Hep3B. The present results suggest that the upregulation of claudin-3 and -4 protein levels by ${\beta}-lapachone$ occurs by a post-transcriptional mechanism and points to a novel mechanism by ${\beta}-lapachone$.

• Korean Journal of Microbiology
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• v.44 no.3
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• pp.193-198
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• 2008

ErmSF is one of the proteins which are produced by Streptomyces fradiae to avoid suicide by its autogenous macrolide antibiotic, tylosin and one of ERM proteins which are responsible for transferring the methyl group to $A_{2058}$ (Escherichia coli coordinate) in 23S rRNA, which reduces the affinity of MLS (macrolide-lincosamide-streptogramin B) antibiotics to 23S rRNA, thereby confers the antibiotic resistance on microorganisms ranging from antibiotic producers to pathogens. ErmSF contains an extra N-terminal end region (NTER), which is unique to ErmSF and 25% of amino acids of which is arginine known well to interact with RNA. Noticeably, arginine is concentrated in $^{58}RARR^{61}$ and functional role of each arginine in this motif was investigated through deletion and site-directed mutagenesis and the activity of mutant proteins in cell R60 and R61 was found to play an important role in enzyme activity through the study with deletion mutant up to R60 and R61. With the site-directed mutagenesis using deletion mutant of 1 to 59 (R60A, R61A, and RR60, 61AA), R60 was found more important than R61 but R61 was necessary for the proper activity of R60 and vice versa. And these amino acids were presumed to assume a secondary structure of $\alpha$-helix.