• Journal of the Korean Society of Tobacco Science
• /
• v.11 no.1
• /
• pp.65-69
• /
• 1989

This study was conducted to obtain the breeding Information for varietal difference of leaf breakdown of flue-cured tobacco at Taegu Experiment Station, Korea Ginseng & Tobacco Research Institute In 1988. The experiment was designed in randomized block with 3 replications. And data were analyzed as spilt plot design composed with varieties for main and growth stages for sub-plots. Among the 8 varieties (NC 95, SL 72, Sleight G-28, TC 518, NC82, NC 2326, NC 567 and TC 499), first half derived from NC 95, and 4 latter varieties were used for check Plants and not derived from NC 95. Plant seedlings were transplanted in 15 April. The number of breakdown leaf were investigated twice at peak growth stage, 5 June and at early harvest stage, 30 June. NC 95 and varieties derived from NC 95 had showed significantly different on the number of breakdown leaf compared to the latter 4 ones after heavy rainfall, but there were not significantly different at the two growth stages. It was suggested that NC95 and varieties derived from NC 95 had substance to induce leaf breakdown by conditional genes after water absorption to plant tissue. Among the varieties derived from NC 95 cultivar SC 72, Speight G-28 and TC 518 had appeared significantly different on the number of breakdown leaf, respectively. These results could appreciated analogically that character of leaf breakdown were governed by heteromeric genes In NC 95.

• Journal of the Korean Society of Tobacco Science
• /
• v.14 no.2
• /
• pp.126-132
• /
• 1992

This experiment was conducted to examine estimates of heterosis, degree of dominance, heritability and phenotypic and genotypic correlation for several mutant characters in flue-cured tobacco variety. Significant heterosis was noted, recording 2.73 and 6.16% for leaf width, -6.86 and -4.72 for leaf shape index, 19.8 and 23.4% for bacterial wilt disease index in Fl an4 F2 generation, respectively, and -1.44 for days to flower in F2 generation. And also leaf width was appeared partial dominance in Fl, and leaf shape index in Fl and F2, Leaf width in F2 was appeared overdominance. Estimated heritabilities in the broad sense ranged from 0.77 to 0.87 for plant and stalk height and days to flower, while those of leaf number, leaf length and width, leaf shape index and bacterial wilt disease index ranged from 0.50 to 0.65. And the heritabilities in the narrow sense were appeared 0.64 and 0.72 for stalk and plant height, respectively, and the others were ranged from 0.32 to 0.47 Positive genotpic correlations appeared among plant height, stalk height, leaf length and leaf width, and between leaf shape index ands day to flower. And negative genotypic correlations appeared between leaf shape index and plant height and stalk height and leaf width, and between bacterial wilt disease index and leaf length.

• Journal of the Korean Society of Tobacco Science
• /
• v.33 no.1
• /
• pp.21-27
• /
• 2011

Amadori compounds(1-deoxy-1-amino-2-ketoses) are important precursors of color, flavor and aroma produced in foods. Amadori compounds occur naturally in tobacco. The contribution of amadori compounds to smoke quality has been (of) interest because of their roles of the Maillard reaction in the leaf chemistry. The amounts of these compound in tobacco are affected by the processes of aging, drying and storage conditions. In this study, eight compounds were chemically synthesized because amadori compounds (have not been sold commercially these days.) were not available for obtaining commercially. The aim of this study was to develop the analytical method of amadori compounds in tobacco leaf by the liquid chromatography mass spectrometry using triple quadrupole analyzer(LC-MS/MS). This method was simple, rapid, selective and sensitive, and eight amadori compounds were simultaneously and quantitatively analyzed within 20 minutes. This method showed excellent accuracy and precision. Recovery rates of amadori compounds ranged from 86% to 102%, with relative standard deviation(RSD) ranged from 2.6% to 5.9%. This method was applied to analysis of amadori compounds contents of tobacco leaves in different varieties. Furthermore, it was expected that the method could be extended to the analysis of other amadori compounds.

• Journal of the Korean Society of Tobacco Science
• /
• v.11 no.2
• /
• pp.127-134
• /
• 1989

Several growth characteristics of two ornamental tobacco species, Nicotiana sanderae and N. affinis, were investigated in this study. Also effect of temperature and daylength on the flowering of the tobacco plants were evaluated to obtain basic information on breeding and cultivation. 1. The plants were great in high temperature-long day at the early stage and in low temperature-short day at the late stage of plant growth, for both Nicotana species. At the early growth stage the leaf length N. sanderae was great in high temperature-long day, and that of N. affinis was great in high temperature-short day period, while at the late stage of the plant growth the leaf lengths were more significantly effected by the temperature rather than daylength. Leaf width and leaf shape index were less sensitive to the conditions. 2. For both of the species, the total number of tobacco leaves not much influenced by the temperature and daylength. 3. There were no significant differences for budding and flowering period between the two species, both of which were sensitive to temperature and daylength with more influence by daylength than temperature. 4. Number of floral stalks, number of flower and flowering period were not much influenced by temperature and daylength; however, N. affinis had 2 more floral stalks, 31 more flowers, and 6 day longer flowering period than N. sanderae.

• Journal of the Korean Society of Tobacco Science
• /
• v.6 no.2
• /
• pp.215-219
• /
• 1984

This experiment was conducted to investigate the effect of physical and chemical characteristics of lower grade tobacco with treatment of ozone. In ozonization of low grade tobacco, optimum moisture was 20 percentage, and decrease ratio of content of tobacco leaf was 30 percentage in nicotine , 15.9 percentage in total volatile base, 10.5 percentage in petroleum-ether-extract, 29.5 percentage in solanesol, and decrease ratio of cigarette smoke composition was 31 percentage in nicotine, 6.08 percentage in tar.

• Journal of the Korean Society of Tobacco Science
• /
• v.9 no.1
• /
• pp.65-71
• /
• 1987

In order to obtain the most suitable ageing term of Flue-cured tobacco in natural condition, leaf tobacco packed in hogshead and stored in leaf tobacco warehouse for 4 years, and the changes of major physico-chemical components were examined at three months intervals, As storage time goes by, except the moisture content, general chemical components of leaf tobacco were decreased until about 9-15 months of storage. Among them, decrement of total sugar was the most significant. After 18 months storage, the chlorophyll was decreased by 50% of initial stage. The content of essential oils, solanone, damascenone, megastigmatrienone I, II, III, IV, were approached the maximum between 12 months and 15 months of storage and then lessened by degrees.

• Journal of the Korean Society of Tobacco Science
• /
• v.17 no.2
• /
• pp.120-125
• /
• 1995

Varietal difference in the occurrence of grey leaves in upland diverted from paddy field were investigated with several flue-cured tobacco varieties in Korea. Grey leaves caused by Fe, Mn and Cl toxicity were occurred in all varieties used in this experiment. The variety, NC82 and KF113, however, were more sensitive than other varieties to the toxicity. It's revealed that grey leaves had little effects on the contents of total sugar, nicotine and total nitrogen. NC82 and KF113 were increased in the ratio of occurrences in the paddy soils which had a tendency to concrete with the passage of days after transplanting. NC82 showed high occurrences of grey leaves in each of variety used and it was estimated that this variety had a traits of easy occurrences of grey leaves, and it might be presumed that there would be a close connection between the ratio of occurrence and amounts of absorbed causal materials accumulated in plants and tobacco varieties on the occurrence of grey leaf tobacco.

• Journal of the Korean Society of Tobacco Science
• /
• v.28 no.2
• /
• pp.75-82
• /
• 2006

This study was carried out to investigate the changes of free sugars, non-volatile organic and fatty acids in flue-cured leaf tobacco during aging. The threshed leaf tobacco(B1O and C1L) produced in 2002 crop year was aged for 21 month in warehouse of Oc-Cheon Leaf Tobacco Processing Factory. The leaf tobacco were sampled at three month intervals for analysis of free sugars, non-volatile organic and fatty acids. The major free sugars of flue-cured were glucose and fructose regardless of tobacco grades. After aging period of 21 months, the contents of glucose and fructose showed a tendency to slightly decrease, and there was no appreciable change in the contents of sucrose in B1O and C1L grades. The major compound of non-volatile organic acid and fatty acid were malic, oxalic and citric, linolenic, linoleic and palmitic acid regardless of tobacco grade. After aging period of 21 months, the contents of malic, malonic, oxalic and fumaric acid showed a tendency to slightly decrease, whereas succinic acid was slightly increased, and maleic acid was not changed in B1O and C1L grades. The decreases in fatty acid contents in B1O and C1L grade tobacco loaves after 21 month aging were 16.5% and 9.8%, respectively. The decreases in linolenic acid contents in two grades were the highest, showing 22.1% and 12.0% reduction after 21 month aging.

• Journal of the Korean Society of Tobacco Science
• /
• v.14 no.2
• /
• pp.116-125
• /
• 1992

This experiment was conducted to examine characteristics of agronomic characters and estimate of gene effect for several mutant characters. The genetic populations were derived from cross between 83H-5 and Hicks. There were significant difference for plant height, stlk height, leaf shape and bacterial wilt disease index except leaf number, leaf length, and what is more, F3 variance is more than Bl and B2 generation from cross 83H-5 X Hicks. Gene actions for stalk height and bacterial wilt disease were estimated by 3-parameter, and by 6- parameter model for all characters except above two characters but stalk height and bacterial wilt disease index are not significant in the additive and dominance effects. Dominant$\times$dominant epitasis for plant height, dominant and dominant$\times$dominant epistasis for leaf length, additive and additive$\times$additive and dominant$\times$dominant epistasis for leaf width, and additive and additive$\times$dominant epistasis for days to flower were appeared significant in gene action.

• Journal of the Korean Society of Tobacco Science
• /
• v.24 no.2
• /
• pp.88-93
• /
• 2002

This study was carried out to investigate the changes of chromatic, chemical and organoleptic characteristics of green leaf tobaccos during storage in warehouse. Eight grades(flue-cured ; A2O, B2O, C2L and D2L, burley ; A2T, B2T, C2W and D2W) of green loaves produced in 2001 were stored during 8 months(Oct. 30, 2001 to Jun. 30, 2002) in warehouse of Kimcheon(flue-cured) and Namwon(burley) Leaf Tobacco Processing factory, respectively. Moisture contents of D2L in flue-cured and four grades in burley were significantly decreased during storage in warehouse. Redness(a) values of six grades except for D2L and D2W were significantly increased during storage. Lightness(L), yellow(b) and pH values, and the contents of nicotine, total nitrogen and total sugar were not changed during storage. Irritations of flue-cured tobacco were increased, whereas tastes were decreased during storage. Irritations and tastes of burley tobacco were little changed during storage. The result suggests that the flue-cured green leaf being processed as early as possible for minimizing the deterioration of smoke taste during storage in warehouse.