• Applied Biological Chemistry
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• v.45 no.4
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• pp.212-217
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• 2002

We purified polyphenols from persimmon leaf and tested their biological activity. The 60% acetone extract was lyophilized and applied to test enzyme inhibition of glucosyltransferase and tyrosinase. GTase was 82.4% inhibited at $1.8{\times}10^{-1}$ mg/ml and tyrosinase 21.7% inhibited at 0.8 mg/ml. The acetone extract was fractionated into F-1, 2, 3, 4, 5 by Sephadex Q-50 gel filtration and the fraction-1 and 2 showed higher enzyme inhibition activity than the other fractions. To the Proteinase K treatment and autoclaving of the two fractions had no effect on the enzyme activity, but these results suggested that active fraction was not protein but phenol ring completed compounds. By Sephadex LH-20, MCI-gel and Bondapak $C_{18}$ column chromatographies, compouds 1, 2, 3 and 4 from F-1 fraction, compounds 5 and 6 from F-2 fraction and compounds 7 , 8 from F-3 fraction were purified and re-crystallized. The purified compounds was assumed to be condensed tannins of frame flavan-3-ol frame on the basis of color reagent reaction and to be a mixture of monomer, dimer and trimer according to TLC analysis.

• Journal of agriculture & life science
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• v.50 no.5
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• pp.51-60
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• 2016

This study was selected drought tolerant plants, by observing the physiological characteristics and biochemical materials from the 9 kinds of the Compositae plants. After selecting plants of the similar size, and then drought stress was induced by the irrigation stopping. Survival rates, chlorophyll values, relative water content(RWC), excised-leaf water loss(ELWL), proline, reducing sugar were measured after 30 days of stopping irrigation. The species that had high rates of survival were Ainsliaea acerifolia Sch. Bip, Aster koraiensis, Aster scaber, Dendranthema zawadskii(S), however other 5 species were dead. The remaining factors have been determined based on plant species showed a higher survival rate. However, chlorophyll content showed high values in A. acerifolia, A.altaicus var. uchiyamae, A. koraiensis, and will have been determined that has no correlation with survival rates, except for A. acerifolia and A. koraiensis. On the other hand, A. scaber, A. acerifolia, A. koraiensis were determined to be relatively high drought tolerant plants in RWC, ELWL, proline, reducing sugar, it showed a similar correlation with survival rate. As a result of 9 kinds of the Compositae plants A. scaber, A. acerifolia, A. koraiensis were considered relatively higher drought tolerant plants.

• International Journal of Industrial Entomology and Biomaterials
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• v.2 no.1
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• pp.27-35
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• 2001

This study was carried out to investigate the mineral content in the regional, varietal and seasonal mulberry leaves. On average, mulberry leaf samples contained minerals in the order of potassiumi phosphorus, calcium, magnesium, iron and so on (2.494 g/100 g, 2.255 g/100 g, 1.835 g/100 g, 0.627 g/100 g, 0.0245 g/100 g DW, respectively). Calcium content was 19-fold and 4-fold higher than that of green tea and spinach, respectively, suggesting that mulberry loaves appear to be a calcium-rich food source. In the comparison of geographic samples of Cheongilppong variety, calcium bevel was highest in Youngchun sample (2.477 g/100 g) and highest potassium level in Suwon sample (2.962 g/100 g). In the geographic samples of YK209 variety, Jinju-City sample was highest in calcium content (1.509 g/100 g). Among wild mulberry loaves collected from Cheju Island and Tsushima, potassium level was highest in Bongge-dong, Cheiu City (3.865 g/100 g) and calcium level in Mitshshima Town, Tsushima (2.948 g/100 g). In the comparison of varietal samples collected in Suwon at the mulberry field of Dept. of Sericulture & entomology, Shinkwangppong variety was highest in the potassium levels although Keryangppong and Shinkwangppong were higher in calcium revel. In the comparison of seasonal samples of Cheongilppong, there was a rough trend of increase in some minerals up to July (e.g., calcium rind )potassium). Finding of the highest calcium and potassium contents in the wild mulberry (3.865 g/100 g and 2.948 g/100 g, respectively) rather than in the cultivated ones warrant that more focus should be paid to wild mulberry leaves to utilize their minerals effciently.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2011.10a
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• pp.13-13
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• 2011

보이차의 주요산지는 중국 운남성이며 특히 란창강 유역이 그 중심지이다. 천연 사포닌과 미네랄류를 풍부하게 포함하고, 지방의 용해, 다이어트 효과, 소화 촉진, 정장 작용, 숙취 해소, 위가 더부룩할 증상 개선, 혈당치 상승 억제, 혈액 순환 촉진에 효과가 있는 것으로 알려져 있다．또한, 면역력 강화 효과와 노화예방, 암 예방효과도 있는 것으로 알려져 있다. 보이차의 기능성 물질은 일반적으로 메탄올 또는 에탄올 용매에 의하여 추출한다. 용매에 의하여 추출하기 전에 메카노케미스트리 분쇄기술을 적용하여 전처리하면 재료의 표면적을 증가시키고 부분적으로 화학적 성분을 변화시켜 기능성 물질의 추출효율을 증가시킬 수 있다. 본 연구에서는 메카노케미스트리 분쇄기술을 적용하여 보이차를 전처리한 다음 메탄올과 에탄올 용매에 의하여 기능성 물질을 추출하여 메카노케미스트리 전처리가 항산화 물질의 추출효율에 미친 효과를 분석하였다. 보이차는 메탄올과 에탄올 추출 전에 유성밀에 의하여 분쇄되었으며 분쇄 후의 형상을 SEM 현미경으로 분석하였다. 아질산성질소 소거능은 pH 1.2에서 메탄올 용매추출을 했을 때 전처리하지 않는 경우 63.0-83.0%로부터 메카노케미스트리 분쇄기술을 적용한 전처리를 한 경우에 74.0-92.0%로 증가하였다. 전자공여능은 DPPH(1,1-diphenyl-2-pycrylhydrazyl)를 이용한 방법으로 측정하였으며, 메탄올 용매추출의 경우에 13.12-49.29%로부터 메카노케미스트리 전처리 후에 15.12-64.29%로 증가하였다. DPPH radical 50% 소거능을 나타내는 $IC_{50}$은 전처리하지 않는 경우 164 ug/mL로부터 전처리한 경우에 151ug/mL로 감소하였다.

• Journal of Life Science
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• v.22 no.12
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• pp.1595-1599
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• 2012

Cell cycle activation and progression in vascular proliferative disease represent potent therapeutic targets. Luteolin, which occurs as glycosylated forms in celery, green pepper, perilla leaf, and camomile tea, has demonstrated antimutagenic, antitumorigenic, antioxidant, and antiinflammatory properties. In this study, we investigated the effect of luteolin on the proliferation of primary cultured rat aortic vascular smooth muscle cells induced by 5% fetal bovine serum. Luteolin at concentrations of 5, 20, and $50{\mu}M$ significantly inhibited this proliferation by 29.6, 50.8, and 83.1%, respectively. The incorporation of $[^3H]$-thymidine into DNA was also inhibited by 25.8, 57.6, and 81.0%, respectively. Flow cytometry analysis of DNA content revealed that FBS-inducible cell cycle progression was blocked by luteolin. Luteolin showed no cytotoxicity in VSMCs in this experimental condition according to WST-1 assays. Luteolin may represent a potential anti-proliferative agent for treatment of angioplasty restenosis and atherosclerosis.

• Korean Journal of Medicinal Crop Science
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• v.25 no.5
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• pp.290-295
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• 2017

Background: Senna tora is a flowering plant in the legume family Fabaceae. Its seeds are roasted and consumed as tea in Asia, to reduce inflammation in the liver and improve eyesight. Thus, it has been considered as an important medicinal crops in Asia. However, breeding trials to improve its genetic properties are rare. Mutation breeding by gamma ray is known to be an effective and highly successful approach for the generation of agronomically useful cultivars. Here we analyzed the effects of several dosages of gamma ray on the biological conditions of Senna tora seeds. Methods and Results: The germination rate and growth patterns of Senna tora were examined following irradiation with gamma ray at 100, 200, 300 and 400 Gy. The total phenolic compound contents and antioxidant activities of Senna tora were analyzed. Germination increased at 100 and 200 Gy in the M1 and M2 generations compared with that of the control (M0). The total phenolic compound contents and antioxidant activity of the seeds significantly decreased as the radiation dosage increased above 100 Gy in the M1 generation. Conclusions: Senna tora, irradiated with gamma ray at dosages 100, 200, 300, and 400 Gy, showed maximum germination rate at 200 Gy in the M2 generation. Plant height and leaf size gradually decreased with increasing gamma ray intensity in the M2 generation. The total phenolic compound contents decreased significantly at 400 Gy, and the related antioxidant activity was also decreased as the radiation dosage increased.

• Journal of Forest and Environmental Science
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• v.37 no.3
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• pp.226-234
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• 2021

Two species, P. pyrifolia and P. ussuriensis, of the genus Pyrus native to Korea, are valuable genetic resources that can be used for food, dietary supplement, pharmaceutical, and cosmetics. Bioactive compounds of the plant leaves are the main components that are used for the products. Farmers had cultivated a few individuals from the wild to produce fruits or leaves for traditional remedy or tea; however, bioactive components of their leaves are not tested. We selected some trees from the natural stand that have distinct traits for the improvement program. We investigated the bioassay on the extracts' bioactive compounds and antioxidant capacity from the selected accessions and other accessions, including newly developed cultivars. The contents of the phenolic compounds and flavonoids from the leaf extracts of the selected accessions were higher than the commonly cultivated trees in both species but lower than 'Sanhyang' in P. ussuriensis. The antioxidant capacity was measured using two assay methods, DPPH and ABST. The selected cultivars also had higher inhibitory activity than common trees. The selected accession 'Cultivar 3' in P. pyrifolia had the highest radical scavenging activity than others. Although leaves of the accessions were used only in this study, all three selected individuals have the potential for cultivar in containing high bioactive compounds and antioxidant capacity.

• The Korean Journal of Pesticide Science
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• v.14 no.1
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• pp.54-64
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• 2010

Effects of thirteen essential oils (anise oil, clove oil, marigold, mustard oil, neem oil, quassia, quilaja, rosemary oil, rotenone, tea tree extract, thyme oil, wintergreen oil, and yucca) and caffeine on typical industrial insect, silkworm (Bombyx mori) and two entomopathogenic nematodes, Steinernema carpocapsae GSN-1 strain (Sc) and Heterorhabditis sp. Gyeongsan strain (Hg) were investigated in the laboratory. When 1,000 ppm of each essential oils was treated, neem oil showed the highest insecticidal activity against silkworm. Mortality of silkworm fed on neem oil treated mulberry leaf was 55.3 and 100% 5 and 10 days after treatment, respectively. The silkworm fed on neem oil treated mulberry leaf did not make cocoon and pupa. Weight of cocoon and pupa was low in rotenone treatment showing 0.27 g and 1.01 g, respectively. Mustard oil had the highest nematicidal activity against entomopathogenic nematodes. 20 ppm of mustard oil resulted in 69.0% and 100% mortality of Sc and Hg 3 days after treatment, but 4% and 36% at 5 ppm in X-plate, respectively. Mortality of baited Galleria mellonella larva by Sc was not different from control at the concentration of 100 ppm of mustard oil while 30% lower in Hg in sand barrier. Mean numbers of established infective juveniles of Hg in Galleria larva were lower than Sc in sand barrier. Survival rate of Sc was similar to control at the concentration of <200 ppm of mustard oil in sand barrier.

• Journal of Practical Agriculture & Fisheries Research
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• v.22 no.1
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• pp.65-77
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• 2020

In order to industrialize of Dendranthema indicum (L.) DesMoul., which is a lot of commercially available and is synonymous with chrysanthemum tea, in the autumn of 2018, Dendranthema indicum (L.) DesMoul. seeds were collected from its own native region, and the seeds were germinated after refrigerated storage. Young seedlings were subjected to experiments in February, March, and April in the open field to examine the effects on the harvesting of leaves by distance and the growth of leaves and stems. The results of analyzing the components by collecting the leaves+stem after collecting the flower of Dendranthema indicum (L.) DesMoul. are as follows. 1. When D. indicum (L.) DesMoul. seedlings were planted according to the transplanting date, the number of flowers was 17.1 in the transplanting date in April. The diameter of the flower was 2.9cm, 16ea, 6.5~6.6g in the fresh weight, and the dry weight of the case was 1.1~1.2g. The leaves were 46~47ea in March and April in the planted area, 5.2~5.3cm in leaf length and 3.5~3.6cm in leaf width. 2. When planted D. indicum (L.) DesMoul. seedlings according to transplanting distance, the number of flowers was 16.2 when planted at 20×20cm intervals and, 16.8~17.1 at 30×30~50×50cm intervals. The diameter of the flower was 2.7~2.8cm, the number of petals was 8, the length of the petal was 0.8 cm, and fresh weight was 6.5~6.6g per flower. Leaves had the largest number of 47 of 30×30cm and 40×40cm, and leaf length appeared at the longest 6.2cm in the 50×50cm treatment area, but 5.2cm in the other treatment areas. 3. The extraction yield of D. indicum (L.) DesMoul. leaves+stems was 7.93%, and the extraction solvent colors were light green at 50, 60% and green at 70, 80, 90, 100%. The extraction yield of D. indicum (L.) DesMoul. flowers was 7.58%, the color of the extraction solvent was light yellow at 50, 60 and 70%, yellow at 80 and 90%, and dark yellow at 100%. 4. We confirmed 11 kinds of ingredients such as in D. indicum (L.) DesMoul. flowers are gallic acid, 4-hydroxy benzoic acid, methyl gallate, 4-hydroxy-3-methoxy benzoic, caffeic acid, salicylic acid, p-coumaric acid, sinapic acid, naringin, 4-melthoxyben, flavone. The content was 29.200-36.900ppm. 5. The components contained in the D. indicum (L.) DesMoul. leaf+stem, salicylic acid appeared at 6,129.526ppm, and the next 4-methoxyben was 1,966.714ppm. It was methyl gallate 8.197ppm, 4-hydroxy-3-methoxy benzoic 6.994ppm, caffeic acid 5.566ppm, flavone 4.522ppm, p-coumaric acid 3.787ppm, gallic acid 1.893ppm that appeared in the content below 10ppm.

• Korean Journal of Food Science and Technology
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• v.41 no.5
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• pp.483-489
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• 2009

Herein, we present the results of our investigation of 61 pesticide residues in 100 samples of green teas obtained from Korean markets. Bifenthrin, chlorpyrifos, dicofol, EPN, chlorfenapyr, tebuconazole, cyhalothrin, difenoconazole, and tebufenpyrad were detected in 22 of the 100 green tea samples. The quantity of pesticide residues for bifenthrin was 0.12 ppm (maximum residue limits (MRLs): 0.3 ppm) in one sample, chlorpyrifos was 0.24-0.78 ppm (MRLs: 2.0 ppm) in three samples, dicofol was 1.64-4.19 ppm (MRLs: 50.0 ppm) in two samples, EPN was 0.13 ppm (MRLs: 0.05 ppm) in one sample, chlorfenapyr was 0.01-1.23 ppm (MRLs: 3.0 ppm) in 16 samples, tebuconazole was 0.71 ppm (MRLs: 5.0 ppm) in one sample, cyhalothrin was 0.05-0.3 ppm (MRLs: 2.0 ppm) in five samples, difenoconazole was 0.23 ppm (MRLs: 2.0 ppm) in one sample, and tebufenpyrad was 0.06-0.07 ppm (MRLs: 2.0 ppm) in two samples. More than two pesticide residues were detected in seven samples. All detected pesticide residues (with the exception of EPN) were within the MRLs.