• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.4
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• pp.349-353
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• 2004

Mature embryo and leaf base segment of Korean oat were used as materials in an experiment to check plant regeneration efficiency. MS media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin, and picloram were used for callus induction from mature embryos and leaf base segments. Three mg/l of 2,4­D and 3 mg/l of picloram in callus induction medium showed high frequency for plant regeneration from mature embryos. Leaf base segments were transferred to callus induction medium and incubated at $25^{\circ}C$ in 16/8 hr light/dark cycle for 3 weeks. Callus induction from leaf base segments of Malgwiri showed high efficiency in medium containing 3 mg/l of 2,4-D and 1 mg/l of kinetin $(91.8\%)$. In case of Samhangwiri, the combinations of phytohormones did not show significant difference. Regeneration from leaf base segments showed high frequency in shoot medium containing 1 mg/l of antiauxin, tri-iodobenzoic acid (TIBA) and 1 mg/l of 6-benzyladenine (BA). Calli induced from leaf base segments of Samhangwiri and Malgwiri in media containing 3 mg/l of 2,4-D and 3 mg/l of picloram showed high regeneration frequency. It appears that the callus initiation medium may be an important factor for subsequent plant regeneration.

• Plant Resources
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• v.4 no.1
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• pp.53-58
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• 2001

The effect of carbenicillin on the dedifferentiation and the regeneration efficiency of plant tissues of horseradish(Armoracia rusticana) was evaluated, Inhibition effect for callus initiation was observed when leaf blade, root and petiole segments were grown on MS medium containing 500 mg/L to 2000 mg/L carbenicillin and 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D). The regeneration of horseradish shoots from leaf blade, root and petiole explants were decreased as the addition of carbenicillin increased from 1000 mg/L to 2000 mg/L in MS medium containing 0.5 mg/L of 6-benzylaminopurine (BAP) or kinetin. Especially, 500 mg/L carbenicillin treatment significantly inhibited shoot induction when leaf blade explants were grown on hormone-free MS medium. It was suggested that the toxic effects of combinations of carbenicillin and 2,4-D may be due to high auxin activity levels.

• Journal of Broadcast Engineering
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• v.27 no.6
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• pp.923-935
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• 2022

This paper deals with detection and classification of leaf diseases for phenomics systems. As the smart farm systems of plants are increased, It is important to determine quickly the abnormal growth of plants without supervisors. This paper considers the color distribution and shape information of leaf diseases, and designs two deep leaning networks in training the leaf diseases. In the first step, color distribution of input image is analyzed for possible diseases. In the second step, the image is first partitioned into small segments using mean shift clustering, and the color information of each segment is inspected by the proposed Color Network. When a segment is determined as disease, the shape parameters of the segment are extracted and inspected by proposed Shape Network to classify the leaf disease types in the third step. According to the experiments with two types of diseases (frogeye/rust and tipburn) for apple leaves and iceberg, the leaf diseases are detected with 92.3% recall for a segment and with 99.3% recall for an input image where there are usually more than two disease segments. The proposed method is useful for detecting leaf diseases quickly in the smart farm environment, and is extendible to various types of new plants and leaf diseases without additional learning.

• Journal of Plant Biotechnology
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• v.31 no.1
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• pp.49-53
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• 2004

Shoot formation was investigated from in vitro cultivation of exotic hybrid poplar (Populus koreana ${\times}$ P. trichocarpa) with a specific stomatal character occurring both upper and lower surface of leaves. Two different explants (stem and leaf segment) of Peace poplar were cultured on half strength Murashige and Skoog (MS) basal medium supplemented with the various concentrations of thidiazuron as a plant growth regulator. Most adventitious shoots were produced from excised ends of stem or mid-veins of leaf segments. The highest average numbers of shoots were 7.1 and 5.3 with the treatments of 0.02mg/L TDZ in both explants of stem and leaf segment. The highest shooting rates were achieved to 83.3％ and 47.6％ with the concentrations of 0.01mg/L and 0.02mg/L TDZ by axillary bud and leaf cultures, respectively.

• Journal of Plant Biotechnology
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• v.6 no.2
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• pp.113-118
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• 2004

Somatic embryos were formed from calli obtained from axillary shoots (raised from nodal segments of glasshouse-grown plants under aseptic conditions), internodal segments (from in vitro-raised plants), and root and coty-ledonary leaf segments (from in vitro-raised seedlings) after 8 weeks of initial culture. Embryo formation was the highest (97.33%) from cotyledonary leaf callus on Mura-shige and Skoog's (MS) medium containing kinetin (KN) (3 mg/L). Somatic embryo induction was lesser with different combinations of auxins while it increased to 100% in internodal segment and cotyledonary leaf calli with 6-benzyladenine (BA) (2mg/L) along with 2,3,5-triiodobenzoic acid (TIBA) (2mg/L). The shoots were induced from somatic embryos raised from root, coty-ledonary leaf and internodal segment calli grown on MS medium containing BA in combination with indole-3-acetic acid (IAA). Maximum of 66.67% cultures formed shoots on MS medium containing BA (1mg/L) in combination with IAA (2mg/L). The shoots raised from somatic embryos were rooted on MS medium supplemented with indole-3-butyric acid (IBA) (2mg/L). The plantlets transferred to the field showed 70% survival rate after one year.

• Journal of Plant Biotechnology
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• v.30 no.2
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• pp.207-214
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• 2003

This study was carried out to establish a novel bioassay system for screening of compounds affecting aromatic amino acid or anthocyanin biosynthesis through investigating a degree of sucrose-induced anthocyanin formation such as size of plant material, buffer conditions, light intensity and irradiated duration, incubation temp., etc were determined and standard procedure (suitable experimental condition) was set up as follows. The second leaf blade of white corn seeding induced by fluridone treatment were segmented into a size of 5${\times}$5 min. The segments were floated on the solution of 1% sucrose in 1.0mM MES buffer (pH6.0∼6.5) and incubated at 26$^{\circ}C$ for 2days under the continuous light condition(70∼100$\mu$mol m$^{-2}$ s$^{-1}$ ). Anthocyanin in the purpled tissues was extracted with methanol containing 1% HCl and the optical density of the clear supematants was determined at 528mm. Influences of some chemicals were tested using this system. Glyphosate, 5-enolpyruvylshikimate 3-phosphate synthase inhibitor, showed most sensitive response with I$_{50}$ value at 3.3$\mu$M. Dicyclohexylcarbodiimide(DCCD) and parachloromercuribenzenesulfonic acid(PCMBS) had a relatively strong ingibition with I50 value at 7.1$\mu$M and 10.2$\mu$M, respectively. These results show that sucrose-induced anthocyanin formation in white com leaf segment provide a very simple and rapid system for searching new compounds affecting aromatic amino acid or anteocyanin biosynthesis by screening at less than 10$\mu$M.

• Korean Journal of Plant Tissue Culture
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• v.26 no.4
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• pp.245-250
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• 1999

Plantlets derived from leaf blade-segment culture of a variegated tobacco (Nicotiana tabacum L. cv. BY-4) that was induced by a heavy-ion ($^{14}N$) beam irradiation to proembryos, were characterized. When explants from both white and green sections of leaves of the variegated plant were cultured on MS medium containing 0.1 mg/L NAA and 1.0 mg/L BAP, the white sections yielded only white shoots, whereas the green sections generated approximately 47.2% green, 37.4% white and 15.4% variegated shoots. In the F1 generation of a green tobacco derived from the leaf blade-segment culture, the segregation ratio of green to white was 1,651:54. Furthermore, reciprocal crosses showed that all of the progenies was green, indicating that the variegation is not maternally inherited. When the signal intensity of photosynthesis genes was determined by DNA gel blot analysis using the variegated leaves derived from green sections of variegated leaves, there were more of the rbcL, psbA, 16S rDNA and 23S rDNA chloroplast genes in the white sections than the chloroplast genes in wild type and green sections of the variegated plants.

• Korean Journal of Plant Resources
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• v.17 no.1
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• pp.43-47
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• 2004

The effect of plant growth regulators and gelling agents for plant regeneration from leaf segment of Chrysanthemum zawadskii ssp. coreanum was investigated. NAA was more effective than BA for plant regeneration. MS medium supplemented with NAA 1 mg/L was the most effective in plant regeneration. The effect of agar and gelite as gelling agent was compared. Agar(0.8%) was more effective than gelite(0.2%) in plant regeneration. Regenerated shoots was successfully increased by shoot grafting in MS medium supplemented with NAA 0.1 mg/L in vitro, and hardened by shoot grafting in artificial soil mix(Peatmoss : Perlite = 1 : 1).

• Horticultural Science & Technology
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• v.33 no.1
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• pp.18-23
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• 2015

Time-specific responses of flower bud differentiation were investigated in 'Shinko' (Pyrus pyrifolia Nakai) pear grown at different altitudes from July through December 2013 to determine their suitability as scions in a top-grafting system. Flower bud initiation and bud necrosis were monitored on each of three sections of one-year-old shoots: terminal, middle, and basal. Flower bud differentiation s tarted in September in the highlands of the Lishan area, and in J uly in the lowlands of the Zhoulan area. In Lishan, flower bud differentiation was higher in the middle and basal segments; during leaf fall, however, flower bud differentiation occurred rapidly in the terminal segment. In Zhoulan, flower buds began to differentiate from the terminal section of the shoot, and severe flower bud necrosis was noted. In July, flower buds developed normally; however, in early August, some of the buds at the basal segment showed browning. During leaf fall, some flower buds showed symptoms of necrosis with rapid and complete browning. Flower bud necrosis began at the basal segment and progressed rapidly towards middle and terminal sections. Before leaf fall, flower buds fell off when scales swelled. The terminal and middle parts of the current-year shoots, with some flower buds, collected in October or later from the Lishan area could be used as scions for top-grafting of 'Shinko' pear. Each grafting scion was a 3-5 cm shoot with one flower bud. These results suggest that scions from the terminal and middle segments of stems of 'Shinko' pear from the Lishan area can be used as scions whereas those from Zhoulan area show necrosis and might not be suitable as scions.

• Horticultural Science & Technology
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• v.17 no.6
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• pp.765-767
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• 1999

Experiments were conducted to find out the optimum cultural conditions for adventitious shoot regeneration from leaf segments of Gypsophila paniculata L. Thidiazuron (TDZ) was remarkably effective for the regeneration of leaf segment in Gypsophila paniculata compared with BA and kinetin. TDZ showed the highest rate of regeneration at $3.0mg{\cdot}L^{-1}$, while kinetin did not affect the regeneration. BA in the medium increased vitrification. Shoot formation efficiency was much higher on $0.3mg{\cdot}L^{-1}$ of IAA-containing media than NAA-containing media. Regeneration of leaf segments was induced with the agar concentrations of 1.0, 1.2 and 1.6%. Dark treatment at the initial stage of the culture increased the rate of regeneration up to 75%. The leaf explants from the 3rd subcultured stock plants after meristem culture, showed the highest adventitious shoot regeneration efficiency.