• The Korea Journal of Herbology
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• v.26 no.3
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• pp.57-63
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• 2011

Objectives : Peucedani Radix ('Jeonho' in Korean) is the root of Peucedanum praeruptorum or Angelica decursiva. However, the root of Anthriscus sylvestris has usually been distributed as Jeonho. This study was performed to determine the discriminative criteria of Jeonho, focused on distribution in Korean markets. Methods : We have determined identification keys to discriminate each Jeonho samples, through observation of external morphology of original plants, and the research of external and internal morphological features of dried Jeonho herbs. Results : Because of identical to the Korean plant name 'Jeonho', Anthrisci Radix had been came into use as a substitute of Jeonho in Korea during the time of Japanese colonial rule. The original plants of Jeonho and its substitute are discriminated with shape of leaf, leaf margin and color of stem. External morphological features of the medicinal herbs of Jeonho are different in the color of cross-sections, pellucid dot, white powder. Internal morphological points, such as fiber bundle of xylem, seconadary mudullary ray and ray of xylem were also used as discriminative criteria for Jeonho. Further details(e.g. identificaion keys) are in the article. Conclusions : We think that these discriminative criteria will be meaningful in identifying the substitutes and adulterants of Jeonho.

• Korean Journal of Plant Resources
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• v.25 no.2
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• pp.257-270
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• 2012

The taxonomic relationship of 3 taxa in $Carex$ sect. $Siderostictae$ were revealed by morphological and molecular studies. The morphological characteristics of Korean $Carex$ siderosticta Hance, which is a type species of sect. $Siderostictae$, was first reviewed in comparison with previous records in order to identify the difference. Then the distribution map of this species was formulated through previous floral literatures and voucher specimens. Although the leaf shape which is represented by length/width ratio showed a great variation depending on habitat condition, the rhizome creeping patterns, the presence of hair on the surface leaf margin and perigynium were shown to be good diagnostic characters to define delimitation of species level. The results from molecular study supported $C.$ $ciliatomarginata$ Nakai should be ranked independently on species level.

• YAKHAK HOEJI
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• v.47 no.2
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• pp.57-68
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• 2003

Under the active search for biologically active novel agents for cancer prevention and treatment, some agents have been found from plants which are easily available. Our previous research on them revealed that C. annuum L. var. angulosum Mill have high antiproliferating effect on cancer cells. However, it has not been known whether the anticancer efficacy is different according to each part of C. annuum L. var. angulosum Mill or whether it can be changed by timing of harvest or solvent for extraction. Thus we compared the efficacy of each part of C. annuum L. var. angulosum Mill and assessed how much difference in the efficacy can be made according to the time of harvest or solvents for extraction. We observed the morphologic change and apoptosis 48 hr after treatment with the extract of each part of C. annuum L. var. angulosum Mill in MCF-7 mammary gland adenocarcinoma cells and human hepatoma cells. We also counted cancer cells by trypan blue method and MTT method to check the cytotoxicity. The leaf extract showed the highest anticancer effect among all the parts of C. annuum L. var. angulosum Mill; 50％ and 70％ reduction in the number of cancer cells was observed at 25 $\mu\textrm{g}$/mι and 50 $\mu\textrm{g}$/mι, respectively. It was more than 2 times as potent as 5-fluorouracil (5-FU). We found chromosomal fragmentation, clumping, and destuction by PI staining, and DNA fragmentation by electrophoresis. In conclusion, this study suggests that leaf extraction using water as solvent has the highest antiproliferative and apoptotic activity in cancer cells compared with other parts of extraction.

• Korean Journal of Environmental Biology
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• v.41 no.4
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• pp.677-682
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• 2023

Milky white spots appeared on red bean leaves in a red bean cultivation area located in Jangyeon-myeon, Goesan-gun, Chungbuk Province. After culturing the pathogen in PDA medium, their morphology was observed, and their genes were BLAST-searched in the National Center for Biotechnology Information (NCBI). The pathogen was identified as a fungus called Rhizopus arrhizus. As a result of reinoculating the isolated pathogen on red beans, the same symptoms as those in the isolated leaves occurred. Characteristic colonies of R. arrhizuson PDA medium initially showed a bright color and then changed to dark gray over time, with mostly spherical sporangia. The sporangiospores were spherical or elliptical, mostly irregular, and small in size. Therefore, based on these results, this disease has not yet been reported in red beans and was called red bean brown leaf blight caused by Rhizopus arrhizus A. Fisch (syn. R. oryzae).

• Horticultural Science & Technology
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• v.35 no.4
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• pp.402-409
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• 2017

We performed seed germination tests to investigate the effects of seed sowing orientation on germination viability on peanut (Arachis hypogaea L.) sprouts. Specifically, we assessed the influence of seed sowing orientation on germination rate, seedling weight, and seedling length, as well as the seedling vigor index. The seeds were sown in oak tree sawdust at 3.0 cm depth. Four seed orientations were tested: vertical with the hypocotyl end down, vertical with the hypocotyl end up, horizontal with the hypocotyl end down, and horizontal with the hypocotyl end up. The mean seed germination percentages of the four seed orientations were significantly different (p < 0.01) and ranged from 25 to 91.7%. The vertical orientation with hypocotyl-end-down and hypocotyl-end-up orientations showed the highest (91.7%) and lowest (25%) germination rates, respectively. The vertical orientation with the hypocotyl end down produced the heaviest (4.9 g) seedlings and the longest hypocotyls (4.65 cm). This orientation also produced the longest true leaf + epycotyl (2.15 cm) and had the highest seedling vigor index (197.1). The seedlings had a straight growth pattern, whereas seedlings from seeds sown with the hypocotyl up had an awkward plumular hook shape. Taken together, to produce peanut sprouts, we recommend placing the seeds vertically with the hypocotyl end down because this orientation leads to a high germination rate, high biomass production, and high overall seedling quality.

• Korean Journal of Microbiology
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• v.53 no.3
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• pp.193-199
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• 2017

This study was aimed to evaluate the antimicrobial activity of Penicillium purpurogenum ED76 on several clinically important microorganisms. The endophytic fungus P. purpurogenum ED76 was previously isolated from Swietenia macrophylla leaf. The antimicrobial efficacy of P. purpurogenum ED76 dichloromethane extract was determined via disc diffusion and broth microdilution assay. A kill curve study was conducted and the morphology of extract treated bacterial cells were viewed under scanning electron microscope. The dichloromethane extract showed significant inhibitory activity on 4 test bacteria and 2 test yeasts. The minimal inhibitory concentration of the extract ranged from 125 to $1,000{\mu}g/ml$, which indicates the different susceptibility levels of the test microorganisms to the fungal extract. The kill curve study has revealed a concentration-dependent inhibition for all test microorganisms. With the increase of the extract concentration, the microbial growth was significantly reduced. The scanning electron micrograph of dichloromethane extract-treated Staphylococcus aureus cells showed the total damage of the cells. The cell wall invagination of the bacterial cells also indicates the loss of cellular materials and metabolic activity. The gas chromatography mass spectrometry analysis of the extract also showed that the major compound was stigmasterol, which constitutes 45.30% of the total area. The dichloromethane extract of P. purpurogenum ED76 exhibited significant inhibitory activity on several clinically important bacteria and yeasts. The study proposed a possible mode of action that the extract cause significant damage to the morphology of S. aureus cells.

• Korean Journal of Plant Taxonomy
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• v.32 no.4
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• pp.397-416
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• 2002

A taxonomic study of Viola section Chamaemelanium in Korea, based on morphological characters, was conducted with light microscopy and scanning electron microscopy. Two species, V. orientalis W. Becker and V. brevistipulata (Fr. et Sav.) W. Becker, are recognized by rhizome and cauline habits, trichomes on the ovary and the shape and beards of stigmas. The latter can be split further into three varieties, var. brevistipulata, var. minor Nakai and var. laciniata (Boiss.) W. Becker, according to the leaf morphology in particular. Viola brevistipulata var. laciniata has the most tooth number, and its teeth are irregular erose but the other two varieties are regular sinuate or serrate. As for the size of leaves, V. brevistipular var. brevistipulata is the largest but V. brevistipulata var. minor is the smallest among the varities. Of them, V. brevistipulata var. laciniata is newly recorded in Korea. It was also revealed that both development and arrangement of the beards developed on the ovary and stigma were particularly diagnostic in the identification of the Viola section Chamaemelanium species.

• Korean journal of applied entomology
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• v.41 no.4
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• pp.255-261
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• 2002

Tomato russet mite, Aculops lycopersici Massee (Acari. Eriophyidae) was recorded in Korea and their SEM observations, distribution, host range, development on different temperature, and chemical control method were studied. Tomato russet mite is a microscopic animal (134.9 $\mu\textrm{m}$) with 2 pairs of legs which had 4 rays of feathered craw. Infected leaf turn reddish brown, stem loss hair, become shiny or surface cross-cracked, and shoot wilted or shrunk. Surface of fruit around fruit stalk appeared rusted or cross-cracked. It was found in glass greenhouse at Buyeo and Yuseong in Chungnam Province, Pyeongchang in Gangwon Province, and Chilgok and Guryongpo in Gyeongbuk Province. Convolvulus, black nightshade, chinese lanternplant, eggp1ant, morning glory, petunia, potato, pepper, tobacco and tomato were confirmed as a host plant in inoculation test. It could develop at temperature around $15-30^{\circ}C$, it took 6-11 days from eggs to adult, higher temperature shorten its life cycle. Female lived longer than male and it lived as long as 12 days at 25-28"C. Among acaricides tested, fenazaquin and cypermethrin were most effective.tive.

• Korean Journal of Plant Resources
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• v.33 no.2
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• pp.116-129
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• 2020

The genus Sedum comprises about 400 taxa with worldwide distribution, and about 20 taxa of the genus Phedimus distributed from Asia to Europe. According to some taxonomists, Phedimus and Sedum are two separate genera of the family Crassulaceae; however, in Korea Phedimus has been treated as a subgenus Aizoon within the genus Sedum. This ambiguous taxonomic treatment of the genus Phedimus needs further confirmation. In the study, we investigated morphological characteristics such as the whole plant height, leaf morphology, and seed coat features of these two genera. We believe that these characters are very important to distinguish Phedimus and Sedum. The result indicated that the seed surface pattern was the most useful diagnostic character. The Sedum had a hexagonal pattern with a single nipple protrusion, whereas the Phedimus had a rectangular pattern with papilla on both ends. The results of this study strongly support the separation of Phedimus from Sedum and thus Phedimus need to be treated as an independent genus.

• Korean Journal of Plant Taxonomy
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• v.40 no.3
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• pp.145-152
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• 2010

The external morphological characters of the Korean Oldenlandia were reviewed, and numerical analysis of 25 quantitative characters carried out to evaluate the delimitation of the taxa. Among the qualitative characters, the leaf shape, inflorescence, pilose of insde corolla, expansion of capsule apex, and seed shape were used to delimit 5 species of Korean Oldenlandia. As the result of principal component analysis of quantitative characters, O. corymbosa, O. hirsuta and O. strigulosa were evidently separated on a two-dimensional plot by PC1 and PC2, and O. brachypoda and O. diffusa slightly overlapped. However, O. brachypoda was distinctly delimited from O. diffusa by the shape of calyx lobe, the lengths of pedicel, filament and stigma. Also each of those two species was in a group of its own by cluster analysis.