• Journal of the Korean Professional Engineers Association
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• v.18 no.4
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• pp.12-22
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• 1985

This investigation was attempted to determine changes in chlorophyll. Pheophytin content PH and titratable acidity during young redish Kim chi fermentation. Young redishes divied in to leaf and vein and fermented in dark room for 35 days at 10$^{\circ}C$ after pickling each group. Samples were collected at 5 days interval for the chemical analysis. The following results were obtained. 1. Chlorophyll content in young redish was decreased by decreasing PH during fermentation periods. Chlorophyll content decreased from 11.60mg/g to 7.62mg/g in leaf, from 0.48mg/g to 0.33mg/g in vein respectively during 35 days fermentation periods. In contrast to chlorophyll. pheophytin content increased as chlorophyll content decreased during the period. The ratio of chlorophyll a and b content in young redish about 3 : 1 in leaf and vein and decreased nearly the same ratio during fermentation periods. In case of pheophytin a and b it was increased without any relation with the ratio chlorophyll a and b. In generally, the optimum PH for eating was known 4.3 to 5.0. Young redish Kim chi was reached the optimun PH at 15 to 25 days of fermentation at 10$^{\circ}C$. At this periods. Chlorophyll content varied from 11.32mg/g to 9.30mg/g for leaf and from 0.43mg/g to 3.37mg/g for vein, respectively. 2. Titratable acidity(as lactic acid) was increased to 0.54% in leaf at 20 days fermentation period during Kim chi fermentation. and nearly unchanged until 30days. At the end of fermentation period the titratable acidity was about 0.30％. Titratable acidity was increased as decreasing pH. and the correlation coefficients was -0.92 in leaf and -0.77 in Vien. 3. Correlation between titratable acidity and chlorophll, pheophytin content was not significant statistically.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.23-23
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• 2017

Rice (Oryza sativa L.) is the world's most important cereal crop. In crop plant, chlorophyll content and leaf senescence could affect grain filling and yield. We analyzed a QTL associated with chlorophyll content and delayed leaf senescence using high chlorophyll near isogenic line (HC-NIL). HC-NIL derived from a cross between Oryza sativa cv. Hwaseong as a recurrent parent and wild species O. grandiglumis as a donor parent showed higher chlorophyll content than Hwaseong. To identify QTL associated with chlorophyll content, 58 $F_3$ and 38 $F_4$ lines were developed from a cross between HC-NIL and Hwaseong. For QTL analysis, simple sequence repeat (SSR) markers were used for genotyping and one-way ANOVA was conducted. A QTL for chlorophyll content (qCC2) was detected in chromosome 2 and explained 24.63% of phenotypic variation. The senescence effect of the qCC2 was examined in dark-induced incubation (DII). Detached leaves from Hwaseong and HC-NIL were incubated on 3mM MES buffer (pH 5.8) at $27^{\circ}C$ under complete dark condition. After 3 days of incubation, the Hwaseong leaves turned yellow, but the HC-NIL leaves were green. HC-NIL has higher chlorophyll content with delayed senescence than Hwaseong. These results indicated that qCC2 is associated with stay-green phenotype. To know whether the qCC2 is responsible for leaf functionality, ion leakage test and Fv/Fm measurement were performed. Both experiment results showed that differences were observed between Hwaseong and HC-NIL but it was not statistically significant. These results might suggest that the qCC2 is possibly related to chlorophyll content and non-functional stay-green phenotype.

• The Korean Journal of Ecology
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• v.18 no.1
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• pp.137-146
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• 1995

Acer pseudo-sieboldianum, A. ginnala, A. negundo and A. saccharinum were selected as materials for the studies on the seasonal fluctuation of chlorophyll content and photosynthetic rates. In all the four species during the growing season except in October, the principal component that determined the total chlorophyll content was chlorophyll a. Content of chlorophyll b increased with leaf age, but that of chlorophyll a decreased. In contrast to A. saccharinum and A. pseudo-sieboldianum which showed their maximum chlorophyll content in June, A. negundo, which showed the highest chlorophyll content of the four species, exhibited its maximum chlorophyll content in July, whereas September in the case of A. ginnala. The fluctuation of chlorophyll content was similar to that of air temperature, and it increased till July. But chloprophyll content showed a significant relationship in early stage of leaf development, and there could be any interdependence between them in accordance with the seasonal change, no longer. In all four species light compensation points decreased in accordance with the seasonal change, and the maximum photosynthetic rates were obtained in August. Respiratory rates were shown no significant difference among species, and they decreased according to the adbance of season.

• Journal of the Korean Society of Tobacco Science
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• v.18 no.2
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• pp.138-144
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• 1996

This experiment was conducted to obtain the basic information on the morphological and physiological changes in tobacco leaf during the growth period by measuring the changes of chlorophyll, sugar, lipid and mineral contents in tobacco plant. Leaf length and width have been fully developed at 25 days after leaf emergence. Dry weight was rapidly increased between 10 and 15 days after leaf emergence and reached the highest at 30 days. Crude lipid content, palmitic acid, and the major saturated fatty acid were increased with progressing senescence, while unsaturated fatty acid including linolenic acid was decreased as the senescence was advanced. The total nitrogen content showed the highest value at IS days after leaf emergence. On the other hand, the total sugar content showed the highest value at 45 days after leaf emergence and glucose, fructose and sucrose were decreased with leaf development and increased at the end of senescence. The content of chlorophyll showed the highest value at 15 days after leaf emergence and began to decrease at 30 days after leaf emergence. The contents of p, Cu, Zn, and Fe in tobacco leaves were decreased by the end of senescence after leaf emergence but those of Ca, Mg, and Mn in leaves were increased. Key words : Nicotiana tabacum chlorophyll, fatty acid, senescence.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.1
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• pp.41-45
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• 2004

We have developed and tested a new method for nondestructive estimation of chlorophyll- and nitrogen-contents in rye leaf. It was found that the relation-ships among nitrogen, chlorophyll content and fresh weight were significantly positive correlated. Nitrogen and chlorophyll content were positively correlated whereas correlation coefficients among R, G, R-B and G-B on the basis of photo-numerical values were negative. We have found that R/(R-B) obtained from data of digital camera is the best criterion to estimate the chlorophyll content of leaves. The regression curves of the relation between R/(R-B) and chlorophyll content were also calculated from the data collected on cloudy days. The coefficients of determination ($\textrm{r}^2$) were ranged from 0.33 to 0.99. In this study, the accuracy in estimating chlorophyll content from the color data of digital camera image could be improved by correcting with R, G, and B values. It is suggested that, for practical purposes, the image values estimated with sufficient accuracy using a portable digital camera can be applied for determining chlorophyll content and nitrogen status in plant leaves.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.243-243
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• 2017

Leaf colors of rice can be used to identify stress level due to its adaptation to environmental change. For most leaves green-related colors are sourced from chlorophyll a and b. For most leaves green-related colors are consisted of chlorophyll a and b. Chlorophyll concentration is normally measured using a spectrophotometer in laboratory. In some remote observation fields, it is impossible to collect the leaves, preserve them, and bring them to laboratory to measure their chlorophyll content. The measurement of chlorophyll content is observed through its color. Using CIE chromaticity diagram leaf color information in RGB is transformed into wavelength (in nm). Pheophytin contents were also analyzed in 95% ethanol extracts. In the process of leaf development of rice young seedling, both pigments were compared. Leaf samples from different rice seedling bed is taken, their colors and RGB values are recorded using Photoshop Image Analysis. SPAD-502 values were also measured. The chlorophyll and Pheophytin contents were fully estimated by ${\rightthreetimes}_{avg}$ on CIE chromaticity diagram.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.5
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• pp.852-857
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• 1998

Three kinds of kimchi using Chinese cabbage, leafy radish and mustard leaf were prepared by conventional method and stored at 5$^{\circ}C$ or 2$0^{\circ}C$ for 13 dyas. During storage at both temperatures, changes of the amounts of salt and ascorbic acid, pH and total acidity were determined, and the relationship of the decomposition of chlorophylls with the production of their derivaties was studied. At both storage temperatures, salt concentration of Chinese cabbage kimchi(3.7%), leafy radish kimchi(3.6%), mustard leaf kimchi(3.5%) was relatively constant during the entire storage period. However, pH and total acidity wre fluctuating with the remarkable changes during 3 days of storage. Ascorbic acid content was slowly decreased during the storage period and the decompositin rate of ascorbic and was greater at 2$0^{\circ}C$ than 5$^{\circ}C$. Among the kinds of kimchi tested, mustard leaf kimchi with the slow decomposition rate of ascorbic acid contained relatively high ascorbic acid content, while leafy radish kimchi contained the lowest content. At both storage temperatures, the production of pheophytin and pheophorbide from decomposition of chlorophyll was least in mustard leaf kimchi, but similar production rates in leafy radish and Chinese cabbage kimchi were observed.

• The Korean Journal of Ecology
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• v.10 no.3
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• pp.119-127
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• 1987

On the east slope of the front ranges of the Colorado Rockies, USA, three species of Populus are distributed at different elevations from the Great Plains to the timberline. At elevations 1, 720m (P. sargentii), 2, 335m (P.angustifolia) and 3, 190m (P.tremuloides) chlorophyll cotents of leaves of thses dioecious trees were determined. Total chlorophyll content was in the range of 0.86-1.56mg/g leaf, increasing with elevation as a second-degree polynomial, and the peak content was estimated to be reached at about 2, 800m. Total chlorophyll content was greater in female than in male trees in the two higher elevation species, but not in P. sargentii, the low elevation species. Chlorophyll b content was greater than chlorophyll a content in the same two higher elevation species. The increase of chlorophyll b than to increase of chlorophyll a content. These phenomena appear to be the result of adaptation of these species to different environmental conditional at different elevation. Sequential distribution of plant populations on environmental gradients in such a way that variation in given plant characters paralell the environmental gradient must occur not only in ecotypes of a species, but also in different but closely related species.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.3
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• pp.377-381
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• 2001

Chlorophyll a and b, pheophytin a and b and $\beta$-carotene, crude chlorophylls and carotenoids (CCC) extracts of mustard leaf kimchi were isolated by DEAE-sepharose CL-6B and Sepharose CL-6B colume and TLC. The effects of chlorophyll a and b, pheophytin a and b and $\beta$-carotene on linoleic acid autoxidation were examined by the determination of peroxide value and conjugated dienoic acid content. Among them, chlorophyll a showed greater antioxidative activity than others, followed by chlorophyll b, pheophytin a, pheophytin b and $\beta$-carotene. Degradation of pheophytin b was observed to be slower than others and $\beta$-carotene showed highest degradation level during the autoxidation reaction of linoleic acid.

• Journal of Bio-Environment Control
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• v.14 no.3
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• pp.144-148
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• 2005

Kale leaf had similar contents of vitamin C, $NO_3$, p, Ca, Mg, and Fe with reported values. Among these internal quality factors, $NO_3$ content which has been a concern recently, ranged from 139 to 429 mg in 100 g fresh kale leaf, Kale showed high vitamin C content ranging from 106 to 203 mg in 100 g fresh leaf. The relative concentration of chlorophyll and b value color had a high correlation coefficient (r) with vitamin C, $NO_3$, Mg and Fe content. The relationship between $NO_3$ content and the relative concentration of chlorophyll was given by the following linear equation: $NO_3$ content : 21.55 + (5.907 ${\times}$ the relative concentration of chlorophyll) with an r of $0.910^{{\ast}{\ast}}$. Correlation between $NO_3$ content and b value was also very dependable (r = $-0.901^{{\ast}{\ast}}$). Vitamin C content and the relative concentration of chlorophyll showed a high correlation, r = $-0.858^{{\ast}{\ast}}$. Among internal quality factors, vitamin C content increased with decreasing $NO_3$ content and their correlation coefficient was high (r = $-0.795^{{\ast}{\ast}}$). Consequently, $NO_3$ content of kale leaf could be inferred from an external nondestructive method, such as the relative concentration of chlorophyll. We may be able to produce high quality kale leaves containing high amount of vitamin C and low content of $NO_3$ using this method.