• Applied Microscopy
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• v.39 no.1
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• pp.57-64
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• 2009

The trapping leaves of Drosera capture insects by secreting sticky mucilage from numerous glandular trichomes (GTs) that are developed on the leaf epidermis. The present study examines and compares the structural features of those trichomes in Drosera binata and D. pygmy with the use of light and electron microscopy. The study focuses primarily on the development and differentiation pattern of the GTs during growth. Upon examination, the upper and lower epidermis were readily distinguishable by the features of GTs in developing leaves. In particular, the GTs were dense in the upper epidermis and along the leaf margin. In D. binata, the capitate GTs with elongated stalk and sessile peltate GTs were found most commonly, whereas only capitate GTs with varying degrees of the stalk length were observed in D. pygmy. Up to ca. $2.2{\sim}3.4\;mm$ long capitate GTs were seen in the leaf margins of D. binata and ca. $3.7{\sim}4.2\;mm$ long GTs having racket-like head with adaxial hemispheric structures, otherwise known as tentacles, were noted in the leaf margin of D. pygmy. The peltate GTs were found to be distributed in the lower epidermis of D. binata. In both species, head cells were dense with cytoplasm containing high numbers of Golgi bodies, ER, mitochondria and small vesicles. Secretory materials accumulated within numerous small vacuoles, then fused together to form a single large vacuole, which serves as a secretory cavity. Flection movement of the marginal GTs and leaf blade GTs, and increased mucilage secretion from the head cells upon contact with prey during the capturing process are considered to be major factors in their active insectivorous mechanism. The findings of this study will be useful in comparisons to similar findings in other species that form adhesive trapping leaves, such as Drosophyllum or Pinguicula., further contributing a better understanding of the function and structure of the trapping leaves of carnivorous plants.

• Korean Journal of Plant Taxonomy
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• v.31 no.4
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• pp.321-341
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• 2001

Phytoliths of leaf blades of Oryza were studied by light and scanning electron microscopy in order to assign the diagnostic character and taxonomic key for the genus. Some phytoliths such as stomatal apparatus, long-cell and short-cell, existing at the same position on the abaxial side of leaf blade, were intensively investigated because of their various forms documented in a previous study. These characters have value either for testing infrageneric classification or for identifying taxa within the genus. Stomatal phytolith is formed by integration of several kinds of sources, such as the guard and subsidiary cell and the papillae. The stomatal phytolith, characterized by not only the absence and presence of phytolith originated by the papillae developed on the guard and subsidiary cell but also their pattern of arrangement, shows various morphologies, and these features have congruent with the infrageneric classification such as section and/or series. Long-cell phytolith is characterized by the absence/presence, arrangement and morphology of phytoliths originated by the papillae on the cell surface. These features may hardly have any systematic relevance within the genus, but contain some informations for identifying of species. All of short-cell phytoliths found are silica body. They form various shapes like cross, bilobate, saddlelike and trilobate, and these features are consistent with infrageneric classification such as section and/or series. Also, some characters, the absence/presence of band of short cell phytolith within costal strip, the absence/presence of saddlelike phytolith within intercostal strip and the number of band of short cell phytolith within costal strip are various according to taxa, but these features do not fall into infrageneric classification. Some taxonomic keys on the phytoliths of stomatal, long-cell and short-cell were developed by their features, and the agreement between these characters and infrageneric classifications was also discussed.

• Korean Journal of Medicinal Crop Science
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• v.13 no.3
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• pp.122-125
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• 2005

‘Johyun’ was a new adlay variety which was developed from three way crosses of (Suwon-6xOkayama)x UCN300-25 at the Northern Agricultural Research Station, Gyeonggi Province ARES. This variety had purple sheath and green leaf blade in primitive growth stage and green leaf and culm in heading stage. Colors of pistil and seed coat were milky-white and dark-brown, respectively. Plant height of Johyun was 179 cm and shortened 22 cm less than Yulmoo-1 which was tested by check variety. Johyun shortened 8 days of heading date and 15 days of ripening date compared with Yulmoo-1. Yield of Johyun was 320 kg/10a of hulled grains in three regional yield trials and 18% higher than Yulmoo-1. This variety was adaptable to single cropping in central and northern region of Korea.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.28 no.3
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• pp.379-385
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• 1983

Forage productivity of Job's tears (Coix lachryma-jobi L.) and adlay (C. lachryma-jobi var. mayuen (ROMAN.) STAPF) and a hybrid corn (Kwangog) and sorghum-sudangrass hybrid (Pioneer 988) was compared in the southern part of Korea where black streaked dwarf virus' (BSDV) is prevalent. Emergence of Job's tears and adlay was more delayed compared to a corn or sorghum-sudangrass hybrid under the drought field conditions. Early growth and regrowth of Job's tears and adlay were less than those of sorghum-sudangrass hybrid. Fresh weight, dry weight, and digestible dry matter of Job's tears and adlay cut once approximately 40 days after flowering were much higher than those of two cuts at every flowering stage. Dry weight of one-cut Job's tears and ad lay harvested 40 days after flowering was similar to the total dry weight of three-cut sorghum-sudangrass hybrid but higher than that of silage corn. Digestible dry matter production was greater in the order of sorghum-sudangrass, corn, one-cut and two-cut Job's tears and adlay. Corn was severely infected with BSDV, but digestible dry matter was relatively high due to its higher in vitro dry matter digestibility. Among the Job's tears and adlay varieties, a variety of Job's tears with globular fruit shape was best for forage production due to its taller plant height, thick stem, wider leaf blades, higher leaf blade/total dry weight ratio, and higher forage yield. However, hulled grain yield of ad lay varieties was higher than that of Job's tears due to a higher hulled/rough grain ratio although they had similar rough grain yields.

• Journal of Life Science
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• v.9 no.2
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• pp.127-135
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• 1999

Gentiana scabra Bunge var buergeri Max, can be widely used such as cut-flower, ornamental and medicinal plant among the native plants. Microscopic and Scanning electron microscopic observation plant were carried out to estimates the characteristics correlated with germinability and germination process, and the results were as follows. The seeds were germinated just after harvest, and it means that there is no or little dormancy of the seeds. Matured seeds showed higher germinability than those of immatured. As the storage period was longer, immatured seeds showed shorter longevity than those of matured. The average longevity was about 190 days. According to the observation results of morphological changes during the germination, seed coat was expanded with water absorption immediately after seeding, and radicals were observed 2 days after seeding. Root hairs were formed behind the growing tip of the root on 4 days after seeding and cotyledon were unfolded on 6 days after seeding. In the microscopic observation of leaf tissues, it could be easily distinguished between midrib, and leaf blade, and also observed upper epidermis, palisade parenchyma and spongy parenchyma consisted as a sing1e layer. Palisade parenchyma was consisted of a single layer of cell. Stomata were restricted lower surface, arranged as anamocytic type, and surrounded by guard cells. In the microscopic anatomy of stem were consisted of cortical layer, forming a single layer of epidermis and parenchyma, and vascular bundle which was consisted of continuous cylinder of vascular tissues.

• Journal of Plant Biotechnology
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• v.34 no.3
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• pp.253-261
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• 2007

This study was conducted to develop an antibiotics marker-free potato (Solanum tuberosum L., cv. Taedong valley) plant having resistance against two herbicides. Agrobacterium tumefaciens strain EHA105, harboring a binary vector plasmid pCAMBIA3300 containing bar gene under the control of a promoter CaMV35S and linked CP4-EPSPS genes driven by CaMV35S promoter, was used in the current study. The leaf segments of newly bred potato variety (cv. Taedong Valley) was co-cultured with Agrobacterium. Then, the regenerated individual shoots were excised and transferred to potato multiplication medium supplemented with 0.5 mg/L phosphinothricin. The shoots were rooted in MS medium without hormone and obtained putative transgenic plant E3-6. Integration of target genes into the E3-6 plant and their expression was confirmed by PCR, Southern analysis, and ELISA test. The tissue necrosis test on young leaf blade and shikimic acid accumulation test using the tissue of E3-6 plant were conducted to investigate the resistance to glufosinate-ammonium and glyphosate, respectively. The transgenic plants (E3-6) simultaneously showed a high resistance to both herbicides. The same results were surely obtained also in the whole plants foliar-treated with alone or mixture of two herbicides, glufosinate-ammonium and glyphosate.

• Korean Journal of Environmental Agriculture
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• v.5 no.2
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• pp.95-100
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• 1986

A pot experiment was conducted to find out As uptake and critical levels affecting yield loss of rice plant. The arsenic was added to two soils of sand loam and loam in the from of $Na_2HAsO_4,\;7H_2O$ at different As concenterations of 0, 10, 25, 50, 100, 150 ppm, respectively. Rice yields significantly decreased with increasing soil As levels and the critical As levels in soils were estimated to be 6.79 ppm for loam and 2.75 ppm for sandy loam. Yield components also decreased with higher soil As levels and the weight of 1000 grains showed the highest significant correlation with As level in soil. Most of arsenic was retained by the roots and a small amount of arsenic was translocated to the shoots. Arsenic content in plant organs was high in the order of root>stem>leaf blade>leaf sheath>brown rice. The number of sterillized grains also increased with higher As level in soil and it was much higher in sandy loam than in loam.

• Korean Journal of Agricultural Science
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• v.4 no.1
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• pp.26-33
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• 1977

We have studied the responce of nitrogen on the newly bred varieties, Yusin and Milyang #23, and the effects of defoliation at maturing stage on the rate of ripened grains under different nitrogen levels. The results obtained are summarized as follows : 1. Milyang #23 as well as Tongil was found to be highly nitrogen responsive variety and showed good grain maturing percentage. The number of grain per unit area affected the total yield under the high levels of nitrogen application. The variety, Yusin, was less responsive to nitrogen and had also less leaf area than other varieties under the high level of nitrogen application. Yusin also seemed to have low maturing percentage due to unbalanced ratio between photosynthetic area and size of storage. 2. The low internodes and leaf blade of Yusin and Milyang #15 were grown too much under high levels of nitrogen and these caused more lodging and less light penetration in pant canopy. 3. The effects of defoliation at maturing stage on yield was high under the high levels of nitrogen application, especially when defoliation was done early stage of maturing. The effects of defoliation appeared to be greates in Tongil than in Milyang #15.

• Journal of Korean Society of Forest Science
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• v.72 no.1
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• pp.9-15
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• 1986

The taxonomy of the broad-leaf evergreen trees (4 genera, 5 species) of Lauraceae was studied by the morphological characteristics of stipules. The results obtained were summarized as follows; 1) The stipules of Lauraceae were arranged alternately with 5-31 stipules of simple leaf. The venations or stipules were basal reticulate and pinnated vein. The shapes of stipule blade were oblong, oblanceolate, rhomboid and oval. The stipule apex was acuminate and obtuse, the stipule base was attenuate, and the stipule margin was entire margin. The trichomes of stipules were sericeous, tomentose and villous. The five species of Lauraceae could be identified by the morphological characteristics of stipules. 2) The morphologically changing forms of stipule vein were classified into three groups; A form - the form of pinnate vein with the distinguished main vein (lozoste lancifolia), B form - the three vein grows into the form of basal reticulate vein (Neolitsea sericea, Cinnamomum japonicum and C. camphora), C form - the parallel vein grows into the form of basal reticulate vein (Marhilus thunbergii). 3) The changing forms of stipule's width from stipule "a" to "j" were three forms; (1) a>d>g>j form: C. japonicum, C. camphora and I. lancifolia, (2) a>cj,(aj,(a

• Journal of Korean Society of Forest Science
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• v.59 no.1
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• pp.1-8
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• 1983

Classification on the group of Salix pseudo-lasiogyne growing in Korea, was conducted using cluster analysis, factor analysis, and principal component analysis. Thirty-six characters(Table 2) of the 5 basis species were measured. The phenograms and ordination plot showing the relationships between the species were made by applying the cluster analysis and principal component analysis. Five important factors, such as leaf blade width, number of right serration, angle of leaf apex, number of flowers in an ament, and the ratio of petiole length to width, were inferred from the rotated factor matrix, and their state values were presented in polygonal diagram. Salix pseduo-lasiogyne and S. babylonica were similarly correlated and linked in one group, S. dependens and S. matsudana for tortuosa were secondarily linked in the other group. S. koreensis appeared as an aliemated species from each of the two groups.