• Journal of the Korean Society of Tobacco Science
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• v.18 no.2
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• pp.113-119
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• 1996

The ovipositional responses of two strains of the cigarette beetle, Lasioderma serricorne F., to thickness, of air- and flue-cured leaf tobacco were investigated. Eggs laid by the strains reared with wheatflour (95%) + yeast (5%) in the laboratory for several years were not different between air - and flue-cured tobacco leaves. There were negative correlations between grades of cured tobacco and ovipositional response, and the regression equation was Y(ovipositional response) : 58.22 - 10.15X(leaf grade). Adults of strains reared on tobacco leaves laid 161.00±80.29 eggs on the first grade of thick flue-cured leaves, and 7.67 ± 5.51 on the second grade of thin air-cured leaves. There was difference in ovipositional responses among different grades within variety, but significant differences were noted between thick and thin tobacco leaves. Key words : ovipositional response, cigarette beetle, Lasiodema sewicome F., tobacco.

• Journal of Korean Society of Forest Science
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• v.86 no.4
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• pp.407-414
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• 1997

The resistance of a non-transgenic poplar clone, 'Ogy' and three transgenic poplar lines to the cottonwood leaf beetle, Chrysomela scripta F., was evaluated by in vitro feeding. The lines were transformed with neomycin phosphotransferase II(NPT II) as a selectable marker, proteinase inhibitor II(pin2) as a resistance gene, and CaMV 35S as a promoter. An efficient method of sterilizing the beetle eggs and introducing them into plant tissue cultures was developed. The resistance of the transgenic lines was investigated in terms of effects tin leaf area consumed, insect weight, insect developmental stages, and plantlet root dry weight after feeding. Also, leaf area consumed was examined by leaf age as measured through leaf plastochron index(LPI). The leaf area consumed and insect weight were highly significant between transformants and control, and insect development in vitro was significant among the transgenic lines. Larval infestation was the most severe around LPI 4 to 5 which were young leaves. The system provided a quick, highly controlled method to screen developing transgenic plantlets directly.

• Korean journal of applied entomology
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• v.30 no.4
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• pp.258-264
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• 1991

Studies were carried out to investigate effects of temperature and photoperiod on diapause induction and of juvenile hormone III and 20-hydroxyecdysone treatment on diapausing adults of the alder leaf beetle, Agelastica coerulea Baly(Chrγsomelidae: Coleoptera). Its life cycle and ovarian development in adults were also observed. The beetle had one year life cycle with egg, larval, pupal and adult periods being 7-10, 19-21, 14-15 days and about 10 months, r respectively. All adults showed a diapause syndrome when the larvae were reared at $20^{\circ}C$ or $25^{\circ}C$ in combination of photoperiods of 16L/8D, 12L/12D, or 8L/16D. Their ovarioles did not s show any development of vitellogenesis before or during diapause and even when exposed at $15^{\circ}C$ after overwintering. When diapausing adults were treated with JH III they resumed feeding and laid several eggs and broke diapause condition temporally. But diapausing adults treated with 20-hydroxyecdysone did not show any response.

• International Journal of Industrial Entomology and Biomaterials
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• v.23 no.1
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• pp.155-166
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• 2011

The leaf beetle, $Chrysolina$ $aurichalcea$ (Coleoptera: Chysomelidae), is a pest damaging plants of Compositae. In order to understand the genetic diversity and geographic variation we sequenced a portion of mitochondrial COI gene (658 bp) and complete nuclear internal transcribed spacer 2 (ITS2) of the species collected from seven Korean localities. A total of 17 haplotypes (CACOI01~CACOI17), with the maximum sequence divergence of 3.04% (20 bp) were obtained from COI gene sequence, whereas 16 sequence types (ITS2CA01~ITS2CA16), with the maximum sequence divergence of 2.013% (9 bp) were obtained from ITS2, indicating substantially larger sequence divergence in COI gene sequence. Phylogenetically, the COI gene provided two haplotype groups with a high nodal support (${\geq}87%$), whereas ITS2 provided only one sequence type group with a high nodal support (${\geq}92%$). The result of COI gene sequence may suggest the presence of historical biogeographic barriers that bolstered genetic subdivision in the species. Different grouping pattern between COI gene and ITS2 sequences were interpreted in terms of recent dispersal, reflected in the ITS2 sequence. Finding of unique haplotypes and sequence types only from Beakryeng-Islet population was interpreted as an intact remnant of ancient polymorphism. As more samples are analyzed using further hyper-variable marker, further fruitful inference on the geographic contour of the species might be available.

• Korean journal of applied entomology
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• v.31 no.4
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• pp.496-508
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• 1992

Phytophagous insects associated with Dicotyledoneae weeds and host specificities in the field populations were investigated for the survey of biological control agents of weeds in Korea. Fifty four weed species in 39 genera were collected during the survey. The most insects were collected from Polygonales by 24 species in 22 genera and followed by Urticales and Centrospermales by 17 species of 17 genera. The insects collected in the other weed orders were ranged from 1 to 12 species. Out of 17 insect species collected in Urticales, Baris sp. damaged the leaves of Hamulus japonicus in Cannabinaceae as scattered holeshape and showed host specificity. In Polygonaceae, Rumex japonicus and R. crispus were severely damaged by Aphis rumicis and Gastrophysa atrocyanea. G. atrocyanea leaf beetle had host specificity on R. japonicus and ate all the leaves except veins. The leaf beetle, Lypesthes japonicus was a potential biological control agent by feeding leaves of Persicaria spp .. And Lixus spp. were also often collected from Persicaria spp .. Liothrips vaneeckei was first collected from weed, P. modosa. P. senticosa was damaged by unidentified geometrid moth larvae and P. perfoiliata by Miarus atricolor snout beetle. Cassida piperata damaged leaves of Chenopodium album of Centrospermales and showed host specificity. In a soybean field, C. album and Amaranthus mangostanus were severely damaged by Spodoptera litura larvae which were eating soybean leaves. This phenomenon indicates that the presence of weed in cultivated land influences the outbreak of insect pests. Altica oleracea leaf beetle was frequently collected from Oenothera spp. of Onagraceae in Myrtales. Aphis gossyphi was outbroken on Solanum nigrum and Phylliodes brettinghami leaf beetle was first recorded on the same plant. Leaf beetles, Longitarsus scutellais and Hemipyxis plagioderoides were first collected from Plantago asiatica of Plantaginaceae in Plantaginales. They showed host specificities in the fields. The hemipterans were collected from many weeds during the survey and their roles on weeds should be investigated. A tractomorpha bedeli was also collected from many kinds of weeds in forest areas.

• Korean journal of applied entomology
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• v.24 no.1 s.62
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• pp.25-28
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• 1985

Experiments were carried out to investigate the life cycle of the rice leaf beetle (RLB), (Oulema oryzae KUWAYAMA) ,yield losses by its damages, and control method in $1976{\sim}1983$ at the experimental field of Gangweon PORD. Peak occurrence of adult and larva rice beetle was late May and mid-June, respectively. Adult overwintered in the forest of mountain and move into the paddy field to lay eggs on the rice leaf from late seedling stage. A female adult laid eggs for 2 days, and egg, larval and pupal period was 9, 16, and 9 days, respectively. Leersia japonica MAKINO was damaged slightly by the beetle. The yield reduction by artifical defoliation ranged from 6 to 40% and yield reduction increased as leaf feeding delayed until mid-July. All insecicides show 100% of control value against RLB.

• Korean journal of applied entomology
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• v.58 no.2
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• pp.159-164
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• 2019

The purpose of this study was to investigate whether leaf damage in pear orchards caused by large black chafer beetles can be controlled through net traps and clove oil treatment. In June 2017, we measured large black chafer beetle population density and leaf damage rates in two orchards, located in Boseong and Hampyeong, under three treatments: net, net plus clove oil, and control. In order to evaluate the effect of net position on the adult beetles, nets were placed outside the orchard and along the upper and lower parts of a horizontal trellis. The adult beetle population density was 20.4 to 34.7% lower in the net alone treatment, and 21.1 to 38.1% lower in the combined net and clove oil treatment than in the control. The adult beetle population density was 10.9-14.4 times higher outside the orchard and 5.1 to 9.1 times higher in the upper parts of the horizontal trellis than in the lower parts of the horizontal trellis. Leaf damage under both the net only and net plus clove oil treatments was significantly lower than that in the control at both sites. Therefore, intensive net installation around orchards may be a practical organic alternative to prevent leaf damage caused by large black chafer beetles.

• Natural Product Sciences
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• v.23 no.4
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• pp.235-238
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• 2017

Colorado potato beetle is a most destructive insect pest of potato throughout the world. Although utilization of chemical insecticides is a main method for management of this pest, their negative side-effects such as threat to humans and the environmental pollution prompted researchers to search for natural alternatives. Recently plant essential oils with low or without side-effects against noun-targeted organisms and with high availability were considered as safe bio-pesticides. In the present study, toxicity of essential oil of Iranian lemongrass, Cymbopogon citratus Stapf, was evaluated against 3th instar larvae and adults of Colorado potato beetle by a leaf dipping method. Results displayed essential oil had notable toxicity against both larvae and adults after 24 and 48 h exposure times. Probit analysis revealed $LC_{50}$ values (lethal concentration to kill 50% of population) with 95% confidence limits were 10.32 (9.17 - 11.72) and 7.76 (6.80 - 8.74) ${\mu}l/ml$ for larvae and 6.27 (4.82 - 8.15) and 4.35 (3.24 - 5.62) ${\mu}l/ml$ for adults after 24 and 48 h, respectively. Based on regression analysis, a positive correlation between log concentration of essential oil and insect mortality was achieved. Results indicated C. citratus essential oil can be candidate as a natural alternative to the harmful chemical insecticides in the management of Colorado potato beetle.

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2002.11a
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• pp.78-78
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• 2002

• Journal of the Korean Society of Tobacco Science
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• v.21 no.2
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• pp.188-194
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• 1999

Cigarette beetle, Lasioderma serricorne F.(Coleoptera : Anobiidae), is one of the most serious insect pest of stored leaf tobacco in Korea. The first adults of their ecto-parasitic hymenoptera, Lariophagus ditinguendus F. (Hymenoptera: Pteromalidae), were emerged in late June in 1992, in early May in 1993 and 1995, in late May in 1994 and 1996. This parasitic hymenoptera had two peak times of adults emergence. The first one was from late June to early July and the 2nd peak time in the Suwon area occurred from late August to early September in 1993, 1994 and 1996, and late September in 1992 and 1995.