• 제목/요약/키워드: leaf and stem

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음나무 잎 및 수피의 진통소염효과 및 아주반트로 유발된 산화적 스트레스에 대한 효과 (In vivo Antinociceptive Antiinflamatory and Antioxidative Effects of the Leaf and Stem Bark of Kalopanax pictus in Rats)

  • 박희준;남정환;정현주;김원배;박광균;정원윤;최종원
    • 생약학회지
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    • 제36권4호통권143호
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    • pp.318-323
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    • 2005
  • The leaves (KPL) of Kalopanax pictus (KP) are used as a vegetable or a functional food in Korean society. The stem bark (Kalopanacis Cortex, KPS) has been traditionally used to treat neurotic pain, rheumatoid arthritis and diabetic disease. This research was undertaken to demonstrate that the leaf extract of KP (KPL) has also the antinociceptive and antiinflammatory effects like the extract (KPS) of Kalopanacis Cortex and to compare the activity levels of several extracts obtained from KP. Antinociceptive and antiinflammatory effects were measured against the extracts described as followings; KPL-1 (the MeOH extract obtained from the leaf shoot of KP collected on May), KPL-2 (the MeOH extract from KP collected on June), KPL-3 (the MeOH ectract from KP with no thorns), KPS-1 (MeOH extract from KPS of a Korean habitat), KPS-2 (MeOH extract from KPS of a Chinese habitat). The antimociceptive test undertaken by acetic acid-induced writhing, hot plate-, and tail-flick methods using mice. The anti-inflammatory test was also undertaken by measuring the edema in the carrageenan-induced test. The order of activity potency in the antinociceptive and antiinflammatory assays was commonly shown as followings: KPL-3>KPS>1>KPS-2>KPL-1>KPL-2. This order was also observed in acetic acid-induced vascular permeability test. The antiinflammatory activity in carrageenan-induced assay was also observed as the following order: KPL-3>KPS- 1>PS-2>KPL-1>KPL-2. In addition, adjuvant-induced rats were used for a model to assess the oxidative stress. Treatment of the rat with the extracts reduced serum thiobarbituric acid-reactive substances (TBARS), hydroxy radical(OH) and superoxide dismutase(SOD) activity caused by FCA together together with the inhibition of hepatic TBARS level and lipofuscin content. The above finding suggests that the leaf extract has the antinociceptive and antinflammatory activity. It is also suggested that KPL-3 with more potent activity than other tested extracts could be developed for a new available biomaterial.

와송 부위별 추출물의 항균활성 (Antimicrobial Activity of the Solvent Extract from Different Parts of Orostachys japonicus)

  • 윤소영;이소영;김꽃봉우리;송유진;김서진;이소정;이청조;안동현
    • 한국식품영양과학회지
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    • 제38권1호
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    • pp.14-18
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    • 2009
  • 본 연구에서는 약용식물인 와송의 항균활성을 조사하기 위하여 와송의 잎, 줄기 및 뿌리를 물과 에탄올의 2가지 용매로 추출한 후, paper disc법과 MIC test를 이용하여 식품의 부패 및 식중독 관련 미생물 10종에 대한 항균활성을 조사하였다. 그 결과 와송 잎, 줄기 및 뿌리의 물 추출물과 와송 줄기와 뿌리 에탄올 추출물에서는 항균효과가 나타나지 않았지만, 와송 잎 에탄올 추출물은 그람 양성균 6종에 대해서는 모두 항균활성을 나타내었으며, 그람 음성균의 경우에는 S. Typhimurium에서만 항균활성을 나타내었고, S. cerevisiae와 P. expansum에 대해 항진균 효과를 보였다. 특히, C. perfringens에 대해서 가장 높은 항균활성을 보여 0.05% 농도에서 균의 생육을 억제하였으며, S. cerevisiae에 대해서도 0.05% 농도에서 균의 생육을 억제하였다. 와송 잎 에탄올 추출물은 $121^{\circ}C$에서 15분간 가압가열처리에도 항균활성이 감소되지 않는 것으로 나타났으며, B. subtilis를 제외한 균주에 대하여 pH $2{\sim}10$까지 처리 후에도 항균활성이 감소 하지 않는 것으로 보아 와송 잎 에탄올 추출물은 열과 pH에 비교적 안정하다고 사료된다. 따라서 와송 잎 유래의 항균성 물질은 식품의 부패 및 식중독 미생물에 대한 항균활성을 가지며 열과 pH에 대해서도 안정하므로 식품의 천연보존제로서 사용이 가능하리라 사료된다.

GROWTH AND FODDER YIELD OF THE Gliricidia sepium PROVENANCES IN FENCE SYSTEM IN DRYLAND FARMING AREA IN BALI, INDONESIA

  • Sukanten, I.W.;Nitis, I.M.;Lana, K.;Uchida, S.;Suama, M.
    • Asian-Australasian Journal of Animal Sciences
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    • 제8권5호
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    • pp.515-522
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    • 1995
  • The field experiment was carried out to study the growth and fodder yield of the Gliricidia sepium provenances in fence system in dryland farming area in Bali, Indonesia for 24 months. The design of the experiment was a completely randomized block arrangement, consisted of 16 treatments (Gliricidia sepium provenances) and 12 blocks with 10 plants per provenance. Of the 16 gliricidia provenances, six were from Mexico (M), four were from Guatemala (G), and one each was from Colombia (C), Indonesia (I), Nicaragna (N), Panama (P), Costa Rica (R) and Venezuela (V). After 40 weeks establishment the gliricidia were lopped regularly 4 times a year, twice during the four month wet season and twice during the eight month dry season at 150 cm height. There were variations (p < 0.05) in stem elongation from 10 to 121 cm, leaf retained from 48 to 105%, leaf shedding from 53 to 86%, branch number from 4 to 7, fodder yield from 281 to 648 g DW/plant, and wood yield from 53 to 179 g DW/plant; and such variations were affected by the seasons. Retalhuleu provenance of Guatemala (G14), Belen provenance of Nicaragua (N14) and Bukit Bali provenance of Indonesia (I) were ranked first, second and third, respectively, measured in terms of stem elongation, leaf retention, fodder and wood yields during the wet and dry seasons.

버어리종, 황색종, 양건종, 담배의 유전분석에 관한 연구 II. $F_2$의 잡종강세, 상관 및 조합능력 (STUDIES ON THE GENERIC ANALYSIS AMONG BURLEY, FLUE-CURED AND SUN-CURED TYPE TOBACCO II. Heterosis, Correlation and Combining Ability of $F_2$ Hybrid.)

  • 한철수
    • 한국연초학회지
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    • 제7권2호
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    • pp.111-121
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    • 1985
  • The present study was carried out to obtain some basic informations of the breeding of tobacco varieties. Genetically divergent 8 varieties, 3 flue-cured, 2 burley and 3 sun-cured tobaccos, were used in half diallel cross. In order to analyze the heterosis, combining abilities, modes of inheritance and correlations for some agronomic and chemical characters, 8 parents and 28 $F_2$ were tested. The results obtained were summarized as follows: The percentages of heterosis for yield, stem diameter, internode length and total sugar content in $F_2$ hybrid were positive , where-as those for the other characters were negative. Yield had significant positive genotypic and phenotypic correlations with plant height, days to flower, leaves per plant, leaf length, leaf width and leaf shape index (Leaf length/leaf width). General (GCA) and specific combining abilities (SCA) for all characters of $F_2$ hybrid were significant. In the effect of GCA, yield was greater in combinations of Bulgaria and Xanthi, days to flower and leaves per plant were those of Burley 21 and Va 528, leaf length and width were those of Coker 139, respectively. In the effect of SCA, the major part of characters were greater than those of others in the combinations of Burley 21, Va 528, Hicks.

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A pathogen-induced osmotin-like protein gene, CAOSMl, from pepper: Differential expression and in situ localization in pepper tissues during pathogen infection and abiotic stresses

  • Hong, J.K.;Jung, H.W.;Lee, B.K.;Lee, S.C.;Hwang, B.K.
    • 한국식물병리학회:학술대회논문집
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    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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    • pp.78.1-78
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    • 2003
  • An osmotin-like protein (CAOSMl) gene was isolated from pepper leaves infected with the avirulent strain Bv5-4a of Xmthomonas campestris pv. vesicatoria. The cDNA encodes a polypeptide of 250 amino acids with a molecular mass of 27, 361 Da. Its amino acid sequence is highly homologous to various osmotin-like proteins from other plant species. The CAOSMl gene expression was organ- and tissue-specifically regulated In pepper plants. The CAOSMl mRNA was intensely localized in the endodermis area of root tissue and in the phloem cells of vascular bundles of red fruit tissue, but not in leaf, stem, and green fruit tissues of healthy pepper plants. Infection by X. c. pv vesintoria, Colletotrichum coccodes, or Phytopkhora capsici iinduced CAOSMl transcription in the leaf or stem tissues. Expression of the CAOSMl gene was somewhat higher in the incompatible than the compatible interactions of pathogens with pepper. The CAOSMl mRNA was prevalently localized in the phloem cells of the vascular bundle of leaf tissues infected by C. coccodes. The CAOSMl gene was activated in leaf tissues by treatment with ethylene, methyl jasmonate, high salinity, cold acclimation and mechanical wounding, but not by abscisic acid (ABA) and drought. These results indicate that the pepper CAOSMl protein functions in response to Pathogens and some abiotic stresses in pepper plants

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Biomass Partitioning during Early Growth Stage of Soybean in Response to Planting Time

  • Seong, Rak-Chun
    • 한국작물학회지
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    • 제47권4호
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    • pp.286-291
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    • 2002
  • Seedling establishment of soybean [Glycine max (L.) Merr.] is a critical factor in production system and cultural practices. The objective of this study was to identify the components of soybean seedling developments encompassing planting dates and cultivars that respond to emergence, early growth stage and dry matter accumulation. Three soybean cultivars, Hwangkeumkong, Shinpaldalkong 2, and Pungsannamulkong, were planted at Baegsan silty loam soil. Planting date was May 13, June 3, and June 24 in 2001. Sprinkler irrigation was accompanied with 30mm after seeding for three planting dates. Soybean seedlings were sampled at the growth stages from VE to V5. Days to emergence of soybean seedlings were taken 8 at May 13 and 5 at June 24 plantings. Emergence percentage was over 90 at three planting dates. May 13 planting took 33 days and June 24 planting was 25 days for reaching V5 growth stage. Cotyledon number was decreased after V2. Significant cultivar difference was found in cotyledon dry weight until V2 which differed in seed dry weights at the planting times. Leaf and total dry weights of soybean seedlings were not differed from V1 to V3 among planting dates and cultivars. Leaf water contents were generally ranged 78 to 85%. Branch was appeared from V4. Leaf/stem ratio among cultivars was similar at five growth stages and gradually increased from 2.1 at V1 to 2.8 at V5. The results based on this experiment indicated that seedling establishment of soybean was continued from VE to V3 growth stages affecting mainly by planting date and soil moisture.

블랙베리(Rubus fructicosus L.) 돌연변이 유전자원의 생육특성과 형태학적 변이 분석 (Growth Characteristics and Morphological Variation Analysis of Mutant Lines Derived from Gamma-ray and Chemical Mutagen Treatments in Rubus fructicosus L.)

  • 류재혁;김동섭;하보근;김진백;김상훈;정일윤;조한직;김이엽;강시용
    • 방사선산업학회지
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    • 제6권3호
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    • pp.257-265
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    • 2012
  • This study was carried out to evaluate growth, morphological characteristics, and sugar content among fifty six blackberry (Rubus fructicosus) genotypes derived from gamma-ray treatment (fifty two lines), MNU treatments (three lines), and cross breed R. fructicosus${\times}$R. parvifolius(one line). While 56 genotypes had similar stem diameter with origin variety, the most of mutants showed reduced leaf size. Also, they showed a wide range of variation in fruit size and one hundred fruit weight compared with origin variety. In fruit size and sugar content, two mutant lines showed large fruit sizes and ten mutant lines showed 20% higher sugar content than the original variety. In the correlation analysis, positive relationships were determined between sugar content and stem diameter, fruit length, leaf wide and fruit length/fruit wide ratio. However, leaf length/leaf wide ratio showed a negative correlation with sugar content. The results will be used as fundamental data for Rubus fructicosus breeding program.

중금속이 애기장대의 생장과 종자발아에 미치는 영향 (Effects of Heavy Metals on Growth and Seed Germination of Arabidopsis thaliana)

  • 박영숙;박종범
    • 한국환경과학회지
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    • 제11권4호
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    • pp.319-325
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    • 2002
  • This experiment was carried out to investigate the effects of heavy metals (copper, cadmium, lead and chrome) on the growth of plant and seed germination of Arabidopsis thaliana treated with various concentrations of heavy metals. Cadmium and chrome among the 4 heavy metals had no effect on the growth of stem even in the concentration fifty times higher than the official standard concentration of pollutant exhaust notified by the Ministry of Environment. The official standard concentration of cadmium, however, stimulated the growth of stem in general, increasing leaf size and surface area, although it had no effect on the length of stem. But the growth of stem was decreased about 18% in the official standard concentration of pollutant exhaust of lead and copper. There was no growth of root in the concentration of lead and copper ten times higher than the official standard concentration. Cadmium and chrome had no effect on the seed germination, but lead and copper decreased the rate of seed germination. Seeds were not germinated in the concentration of copper ten times higher than the official standard concentration and in the concentration of lead fifty times higher than the official standard concentration. From this research three peculiar results were obtained. Chrome in the soil did not have much effect on the plant growth and seed germination of Arabidopsis thaliana. Cadmium stimulated the stem growth in an optimum concentration. But lead and copper reduced the plant growth and seed germination even in a small concentration, especially copper had the worse effect.

Effect of Shading on Rice Growth Characteristics Under Different Temperature Conditions

  • Zun Phoo Wai;Min-Ji Lee;Woon-Ha Hwang
    • 한국작물학회지
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    • 제69권1호
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    • pp.15-24
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    • 2024
  • Environmental factors play an important role in crop growth and development. In recent years, climate change has become a challenge that limits environmental factors. Light is an important environmental factor for photosynthesis in rice. In addition, temperature is one of the most important factors for rice production; thus, a 1℃ increase in temperature because of climate change can affect rice growth and development. Therefore, we investigated the effect of shading on the growth characteristics of rice under different temperature conditions from the vegetative stage to the flowering stage. Plants were grown at three different temperatures: 26℃/16℃ for 21℃, 29℃/19℃ for 24℃, and 22℃/32℃ for 27℃ in a phytotron. A 55% shade treatment was applied after 10 days of transplanting until the flowering stage. Plant height was not affected by the shading treatment. In the maximum tiller number response to shading, a lower tiller number and growth speed of tiller was found in the 27℃ condition. Among leaf characteristics, shading increased the flag leaf area, length, width, and effective leaf area; however, it decreased the leaf number on the main stem, especially at 27℃. In terms of stem characteristics, shading affected culm wall thickness in both varieties. Finally, regarding the panicle characteristics, lower panicle numbers, spikelet numbers per panicle, primary numbers, and secondary numbers per panicle were found under the shading treatment. Most of the desirable characteristics were affected by the shading treatment at 27℃. Overall, these results indicated that shading had a greater effect on rice plant growth at high temperature.

Varietal Difference of Dry Matter Weight of Stem and Leaf in Rape

  • Kwon, Byung-Sun;Shin, Jeong-Sik;Ahn, Gae-Soo
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2003년도 제10차 국제학술회의 및 추계정기 학술발표회
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    • pp.40-42
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    • 2003
  • In order to examine the possibility that oil seed rape could be used as a forage fodder crop and to select the most suitable variety of forage rape at the southern area of Korea, two varieties of oil seed rape currently grown for oil production and six introduced varieties of forage rape with relatively high yield and high nutritional value were grown at the same condition and yield components were observed. Forage rape was superior to oil seed rape in terms of yield components, plant fresh weight and plant dry mater weight. Velox was superior to any other variety of forage rape in these characters. When plant dry matter weight of the rape was subdivided into four components such as a main stem, branch stems, main stem leaves and branch leaves, contribution of these components to plant dry matter weight was in the order of branch stems, branch leaves, the main stem and main stem leaves. Dry matter percentage of the rape ranged from 9.32 to 11.08 percent, which was somewhat low value. There was no significant difference between two groups of the rape in terms of dry matter percentage. Velox showed somewhat higher value in dry matter percentage.

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